LOCUS       NZ_CP082028            11969 bp    DNA     circular CON 10-JAN-2024
DEFINITION  Klebsiella pneumoniae strain KP18-2113 plasmid pKP18-2113-3,
            complete sequence.
ACCESSION   NZ_CP082028
VERSION     NZ_CP082028.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN20928636
            Assembly: GCF_019823895.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 11969)
  AUTHORS   Qin,S. and Dong,H.
  TITLE     Klebsiella pneumoniae strains
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11969)
  AUTHORS   Qin,S. and Dong,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2021) Institute of Drug Discovery & Development,
            Zhengzhou University, 100 Science Avenue, Zhengzhou City, Zhengzhou,
            Henan 450001, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: unicycler v. v.0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 12x
            Sequencing Technology             :: Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/10/2024 03:35:23
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,660
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,368
            CDSs (with protein)               :: 5,368
            Genes (RNA)                       :: 122
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 170
            CDSs (without protein)            :: 170
            Pseudo Genes (ambiguous residues) :: 0 of 170
            Pseudo Genes (frameshifted)       :: 68 of 170
            Pseudo Genes (incomplete)         :: 107 of 170
            Pseudo Genes (internal stop)      :: 25 of 170
            Pseudo Genes (multiple problems)  :: 27 of 170
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP082028.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..11969
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="KP18-2113"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pKP18-2113-3"
                     /geo_loc_name="China"
                     /lat_lon="34.73 N 113.70 E"
                     /collection_date="2018-12-02"
                     /collected_by="Zhengzhou University"
     gene            815..2779
                     /locus_tag="K6164_RS00005"
     CDS             815..2779
                     /locus_tag="K6164_RS00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_071177727.1"
                     /translation="MFNTDSLAWQWHIPELARQIYPWVSWLYREYYSLAGFFLVIGLFF
                     GLAELWAGRMRFVGLSHKARREYADRYKRDNNRTVPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLV
                     AGVLLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSTEQARKQQAVFLGKDEQGQPVLVPRDTWRKTNIQILGLPGSGKSAMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLV
                     AGFNLSETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     KLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFRRWPHASIMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPADFV
                     KTTVLDNTPIRWFYRAASQESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARLAFSSPLRVERREISLKTFPVLAKTDPLAEY
                     LPPADGGGPKADDDFAGLY"
                     /name="TraM recognition domain-containing protein"
     gene            2779..3510
                     /gene="mobC"
                     /locus_tag="K6164_RS00010"
     CDS             2779..3510
                     /gene="mobC"
                     /locus_tag="K6164_RS00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WP_071177728.1"
                     /translation="MTLEQYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRAKYPVDGRQVSVWGLTPHGVAFSFDEDEPLTDVIPFQP
                     SRVSAAQLPHRLAVQSLRLSMEGRGATGWRYLHRMALKGMKVPDALAELDGRTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRRANGIDEIWYICPDRPTLVRVQRAILAVDEIVNPQTGEA
                     RKTVELDRERLFACFKFMTTE"
                     /name="mobC"
     gene            3517..4047
                     /locus_tag="K6164_RS00015"
     CDS             3517..4047
                     /locus_tag="K6164_RS00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_071177729.1"
                     /translation="MALYMVFIEDVEDKDGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGENPQWERDKPTGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQINAMIEVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(4074..4253)
                     /locus_tag="K6164_RS00020"
     CDS             complement(4074..4253)
                     /locus_tag="K6164_RS00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165970.1"
                     /translation="MTKQEKTALNMAKFIKDQSLLLLEKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(4279..4707)
                     /locus_tag="K6164_RS00025"
     CDS             complement(4279..4707)
                     /locus_tag="K6164_RS00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001140599.1"
                     /translation="MAKTLLNLSQAAQAAGITRRTLYNHVKQGKVTVSRDGKNNPVVDV
                     SELIRVYGNVNIPEKQIPGISHRENTQKNFPQEQLLAMQKELADLRQAVTLMLEDKTSR
                     EEERRQHDDERRKLQAEVDRLTTELTQKKKRFWSGWFS"
                     /name="hypothetical protein"
     gene            complement(4871..5185)
                     /locus_tag="K6164_RS00030"
     CDS             complement(4871..5185)
                     /locus_tag="K6164_RS00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001136729.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]; GO:0009432 - SOS response [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_071177730.1"
                     /translation="MPQVTISALAQRDLQRLQDFLKTKNRLAARKGGEVIVRAIQQLKT
                     LPDIGRPVPFLPLEYKELVIGFGDSGYVMLYRHDREMDQIVIVTVRHQKESGYPGADSL
                     "
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(5173..5451)
                     /locus_tag="K6164_RS00035"
     CDS             complement(5173..5451)
                     /locus_tag="K6164_RS00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014542884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family ribbon-helix-helix protein"
                     /protein_id="WP_000093040.1"
                     /translation="MSSATSIRLDEELKDRLKTLADDRHRSAHALMLEAITEYIDREEK
                     RSQYLRDGQAAWQHYQETGLHLTAEEAEAWISTWGTENEQDAPPCHR"
                     /name="CopG family ribbon-helix-helix protein"
     gene            complement(5626..5991)
                     /locus_tag="K6164_RS28215"
     CDS             complement(5626..5991)
                     /locus_tag="K6164_RS28215"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /GO_function="GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="WP_072354022.1"
                     /translation="MKAFTLTVLAAMFLTPVHAIPLKPVTTLQPTYPVQAAAKRIEGQV
                     TATFDILDDGRVDNVSLTGCHLFFRETLTAMRHWLYPAGHPAMRQVINFEFSLKNDGLQ
                     DPRPVTTQKLNDDGCRQ"
                     /name="TonB family protein"
     gene            complement(5988..6359)
                     /gene="tolA"
                     /locus_tag="K6164_RS00045"
     CDS             complement(5988..6359)
                     /gene="tolA"
                     /locus_tag="K6164_RS00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237044.1"
                     /GO_component="GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA]"
                     /GO_function="GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="WP_001237044.1"
                     /translation="MRLIPFILLALCSPLLLTACKHSTTQPPKIYTLDKSSKISSYAAA
                     IKTAVEMQMFEPEAYAGKMCTVRISIQRDGTLNSAIAEGGDPKLCKAAISAVTHAKIPP
                     APDENTWQIFKNAPLDFRP"
                     /name="tolA"
     gene            complement(6633..6878)
                     /locus_tag="K6164_RS00050"
     CDS             complement(6633..6878)
                     /locus_tag="K6164_RS00050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032440458.1"
                     /translation="MTHLKLTPAEEKRLADAFVAQALAMGLKGNKTLPKAVKQQVRERV
                     LRAIQDEREARRAQEMRREKYEATETTFTWQPQRRR"
                     /name="hypothetical protein"
     gene            7522..9009
                     /gene="ltrA"
                     /locus_tag="K6164_RS00055"
     CDS             7522..9009
                     /gene="ltrA"
                     /locus_tag="K6164_RS00055"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226014.1"
                     /GO_function="GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="WP_004178082.1"
                     /translation="MMYGEEKSDSLIVAAKQANNPKGAESVERRSGAKGNAEQPHMRRT
                     QSRESMSQRLSRVREAAKQRKKERFTALFHLLTVEALEAAFLSLSRKAAAGVDGIRWMD
                     YAGNMKNNITDLHRRLHQGSYRAQPGRRHYIPKADGKQRPLGIASLEDKIVQYALVKIL
                     NAVYENDFMGFSYGFRPGRSQHDALDALATGLVRTNVNWVLDADISQFFDRVSHEWLIR
                     FTEHRIGDRRVIRLIRKWLTAGTSEEGQWRATEEGTPQGAVISPLLANIYLHYVFDLWA
                     HQWRRRYATGNVVMVRYADDIVIGFDKRYDARRFRIAMQRRLREFGLTVHPEKTRLMEF
                     GRFAAENRAIRGKGKPETFNFLGFTHISGKDRNGRFMLIRKTRRDRMTATLKAIKDGLR
                     RRWHYSIPEQGKWLRRVVQGYLNYHSVPGNFPTMQKFRTHVTNLWRRALRRRSQKDDTT
                     WTKANKLAAAWLPRVRVLHPWPVERFTARHPRQEPGA"
                     /name="ltrA"
     gene            9114..10799
                     /locus_tag="K6164_RS00060"
     CDS             9114..10799
                     /locus_tag="K6164_RS00060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase domain-containing
                     protein"
                     /protein_id="WP_032440457.1"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSVSGDALSAAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPKMMSK
                     IMTSLPADAVTETPASTLPLDQATVRVRQRVVDVVKDERQHIAVVAGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPSLQVSVPKGVPTAKAPPKGIVAEKGDSRPAGFTAGGNSREAVIR
                     FPKETGQKPVYVSVTDVLTPAQVKQRQEEEKRRQQAWDAAHPEEGLKRDYDKAKAELDA
                     EDKNIATLNSRIASTEKALPGARAAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNEKLKAAQASLNAMNESLSRDKAALTGAMESRKQKEKKAKDAENKLNE
                     EKNKPRKGTKDYGHDYHPVPKTEDIKGLGELKRGDPKTPKQGGGGKRARWYGDKKRKIY
                     EWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPKRNIKKYL"
                     /name="colicin-like bacteriocin tRNase domain-containing
                     protein"
     gene            10809..11066
                     /locus_tag="K6164_RS00065"
     CDS             10809..11066
                     /locus_tag="K6164_RS00065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /GO_function="GO:0015643 - toxic substance binding
                     [Evidence IEA]"
                     /GO_process="GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E3-like toxin immunity protein"
                     /protein_id="WP_032440455.1"
                     /translation="MGLKLRLTWFDKKTEEFKGEEYSKDFGDDGSVIESLGMPLKDNIN
                     NGEFDLKKEWVPLLQPFFKNQIKPDVDNCYIAFDYRDGNW"
                     /name="colicin E3-like toxin immunity protein"
     gene            11151..11300
                     /locus_tag="K6164_RS00070"
     CDS             11151..11300
                     /locus_tag="K6164_RS00070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_032440454.1"
                     /translation="MKKVKTIFLFILIVAGFLLAACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     BGC             4114..11969
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          complement(11404..11969)
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.418"
                     /coverage="98.0"
                     /evalue="0.0"
     Typing          2780..3507
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.416"
                     /coverage="100.0"
                     /evalue="1.35e-140"
ORIGIN
        1 cgttttgcct cacatgttag tcccctgctt atccacggaa tctgtgggta actttgtatg
       61 tgtccgcagc gcccgccgca gtctcacgcc cggagcgtag cgaccgagtg agcgaggagg
      121 cgcaatttcc ccggcacctg caagacggtc accggacgcg ctgatgtggg atattgtccc
      181 ctgattaagt ctttctattc cctgacgatc aattatcagg ataggtgaac catgtcggtt
      241 tttcgctgaa cattttacgt taacctatct ctgttgcttt tttctccttg cggagaaatg
      301 caagtcatcc ccgctggggc gtggctgact tcaggggaaa agaccttgct tacgctggct
      361 ggacgccacc gacgcacggt cttttcccct ctttctggct ctgtccggct ggacgccggc
      421 cacgccagaa attcacccgg cccccgtcag gctcacgcct gaccacccac aaaaatccac
      481 ggtgctggac gcacctgacg aagcagcaaa gctgctcctg attttgcggg tggtcagccg
      541 ctcgctatgg cgaactctga cgggggccgg aacaccggaa aagaccgcac tggggtgccg
      601 ccttttcctc tcacacgctg gggcgtgctc gttgttcctc cggggctgcg ccttttccgc
      661 atggggcgga aagaggctgg cctccgcggt gaaagctgaa cgcaatcacc acggacgtga
      721 ccgccgccgc catgccttgg ggcatgtccg cggcttgtgc agcgggggct gcgcatcacg
      781 ccggtaaacc cgtcgtctga aggagagaga ttgcatgttt aatacggatt cgcttgcctg
      841 gcagtggcat atcccggagc tggcccggca gatttacccg tgggtcagct ggctgtaccg
      901 ggagtattac agtctggccg gtttttttct ggttatcggc ctgtttttcg gtctggctga
      961 actctgggcc ggccgaatgc gttttgtggg gctgagccat aaggcgcggc gggagtatgc
     1021 cgatcgctat aagcgcgata ataaccggac tgtgccattt tcggtgctgg cctcgctgga
     1081 ttcacccggg cggatggtca gggcacggcg acatgtctgg aactacgcgt actggctgac
     1141 cggcgctgcc ggtctggccc ttctgggggg gctggttgtc gccgtgttta tcgagagctg
     1201 gggagaaacc gtcctgcgcc cggacctcta tgcgaacgca gattattacc tgctgcagcc
     1261 ggctaacctg ttcgggatag cctggtgtgc cggagggctt gtcgccggcg tccttctggc
     1321 gcggctgctg gcgttcctgc tgcttgatgg tcattttgcg gccgctgacg aggcggtgaa
     1381 cgcgaaactg aatcaggaga gccggcgcag cagccagcgg accggagaaa tgacagacgt
     1441 ccgccacctg catttcgggg agccggtacc ggttaacgcg ctggccgatt tttccacgga
     1501 gcaggcccgg aaacagcagg cggtgtttct gggtaaggat gagcagggcc agccggtgct
     1561 ggtccctcgt gatacctggc gcaagaccaa tattcagatc ctcggcctgc ccgggagcgg
     1621 caagagcgcg atggccacca acgcgctcat tcgctgcgta cgcgatttcg gggacgctgt
     1681 ggtgtacttc gaccccaaag gggacgcgtg ggcgccgcac gtcttccggg cgcactgccc
     1741 ggacttcacc ctgctggatc tgcggccggg gaaaccggcg cagctgaatc tgttccgcga
     1801 tctcgaccag tacgccctga agaatctgct ggttgccggg tttaacctga gtgagaccgg
     1861 cgacgtggcg gaccactacc gcatcagcga gcagaaagcg gcgaagctga ttgcggagca
     1921 gttcccgcag ggggcaaaca ttcagcaggt gctggcggcg gcgtacgcgt tgccggaggc
     1981 cctgaaaaag gacgtgaagg ggctgatcac caagctggag aacgtggccg acctgagcgt
     2041 cctgcagacg gacagcggta tcgatgtggc ggggataatc aacggcggcg gctgcctgta
     2101 cgtcatcggc tcgatggacg atgaggcggt gatccgggtg cagaagatgc tgtttgcccg
     2161 ctgcgcgcaa atcatcattg cgcgggatga gttccggagg tggccgcacg cgagcatcat
     2221 gctcgatgag attaaatatc tgctgtcgaa gtacgtgctg aacgcgctgg ggacgctgcg
     2281 cagccgggac tgcaacctgc tgctcgcgca ccagtcgctg ggcgacttcg gtcagtgcgg
     2341 gcaggatctg cccgctgatt ttgtgaaaac cacggtactg gataacacgc cgatccgctg
     2401 gttctatcgc gcggccagcc aggagtccgc gcagtgggcg gcgggccaga ccggggagat
     2461 ccgggtggac gtggagcgcc gccgggccag ccgggaggcg gggaacgtgg agcacatcag
     2521 cggcgacagc ttcatccaga aggaggcgcg gccgctgttt gacgtgaaca ccctgcaaca
     2581 cctgcctgac gggttcgcgg tgatgaccgg gctgggcgtg gcgcggctgg cgttcagcag
     2641 cccgctgcgg gttgagcgcc gggaaatttc gctgaaaacc ttcccggtgc tggcgaaaac
     2701 ggacccgctg gcggagtacc tgcctccggc cgacggcggc ggaccaaaag cggatgatga
     2761 ttttgcgggg ctttactgat gacactggag cagtacaacg tcagccacgc gaagcggcag
     2821 gcgcggaacg cggagaaaac acggctgacc ctgcgctggc tgcgggaaga gctgtgttcc
     2881 acggcggagc tggttgcgcg acggctgggg attgcggcgg tacagccggt ttaccgcttc
     2941 ctggacagtc tggtggcgaa agggctgctg gtccgggcga aatacccggt tgacggccgt
     3001 caggtcagcg tgtgggggct gacgccgcac ggggtggcgt tctcgttcga cgaggacgag
     3061 ccgctgacgg acgttattcc gtttcagccg tcaagggtgt cggcggcgca gctgccgcac
     3121 cggctggccg tgcagtccct gcgcctctcc atggagggcc ggggggccac cggctggcgg
     3181 tacctgcacc gtatggccct gaaagggatg aaagtgccgg acgcgctggc ggagctggac
     3241 ggcagaacgg tggcatttga ggtagagcgc accgtcaaga gccgcaggcg gtatcaggaa
     3301 gtggtgtccg ggtatctgtt caaccgccgg gcgaacggca tcgatgaaat ctggtacatc
     3361 tgccctgacc ggccgacgct tgtccgggta cagcgggcga ttctggcggt ggatgaaatc
     3421 gtgaatccac agaccggcga ggcccgcaaa acggtggagc tggaccggga gaggctgttc
     3481 gcgtgcttta aatttatgac aacggagtga gggattatgg cgctgtacat ggtatttatt
     3541 gaggacgtgg aagacaaaga cggcgagcgc tgcattatgc caaccagcga gcatctggaa
     3601 gacatggtgg tgctcgcctg gcagaccggg gcgtatgttt tctgttttgt cgaccgggcc
     3661 cagcatctgg cgatgatgaa atccatcggg atgtatgacg agcggctcaa cagcgacgaa
     3721 aaagccacgt actttttcac cggtgaaaat ccgcaatggg aacgggacaa acccacagga
     3781 atgaagctgt gtgattacct ctatcgaaag gtggagcggt tcgccatcga gaacggtgtg
     3841 caggtggttg atgccgggaa gtgctcaccg gagcagatta acgcgatgat tgaggtggtc
     3901 agcgagcggc acggggaaag cgcggtgaac gagaagctga aagcggagct gatgcgggaa
     3961 tataacagcc gctattttaa cgtgccggtg cctgaactgc cggaggacaa acccgcccgc
     4021 cggtggtggc cgttcggcaa aaaataacgc cagcaccggc atggccggtg ctgttattct
     4081 tccccgagct tatcccgcat cacctgccag agtgtttccg catcctcgtg aagccgttcg
     4141 cacatgtcgg ccggggtatc catgtccagc tcgttcagct tttccagcaa cagcagcgac
     4201 tgatccttaa tgaatttagc catattgagg gcagtttttt cctgcttcgt catgttctcc
     4261 attcccttcg gtaagttctc agctaaacca gccggaccag aagcgttttt tcttctgtgt
     4321 cagttctgtt gtcaatctgt ccacttcggc ctggagtttc cggcgctcat catcgtgctg
     4381 gcggcgttct tcttcccggg atgttttatc ttccagcatc agggtgacag cctgccggag
     4441 atcggccagt tccttttgca ttgccagaag ctgttcctgt gggaaatttt tttgtgtatt
     4501 ttcacggtgt gaaatgccgg gaatttgttt ctcagggata ttcacatttc cgtatacacg
     4561 gatcagttct gacacatcca cgacagggtt gtttttcccg tcacgtgaca cggtaacctt
     4621 tccctgtttt acatgattgt atagcgttct tctggtaatg cccgcggctt gtgctgcctg
     4681 tgaaaggttg agcaatgttt tcgccactgt atacccctga ttttatcggg tgtgaaaggt
     4741 gtgtaatttc tcacggtaaa tgtatcgctg aattttaccc gtttttccgt tcaggatgtg
     4801 gttttctgac gcgctgcgct tgtcaaaccg gcgacggcca cgcaatgaat tgcgcccccg
     4861 tcatcggtta ttagaggctg tctgcccccg gatacccgga ttctttctga tgcctgacgg
     4921 tgacgatcac aatctggtcc atttcccggt cgtggcggta gagcatcaca tagccactgt
     4981 cgccaaaccc gatcaccagt tccttatatt ccagcggcag aaacggcacc gggcggccaa
     5041 tgtctggcaa tgttttcagt tgctggatag cccggacgat cacctcacct ccttttctgg
     5101 ctgccagccg atttttggtt ttgagaaagt cctgaaggcg ttgtaaatcg cgctgtgcca
     5161 gcgcggaaat cgttacctgt ggcatggcgg agcatcctgc tcgttctcag ttccccaggt
     5221 acttatccag gcttccgctt cttccgcagt aaggtgcaga cccgtttcct gatagtgctg
     5281 ccatgcggcc tgtccgtccc gcaggtactg gctgcgcttc tcttcccggt cgatgtattc
     5341 ggtgattgcc tccagcatta gcgcgtgcgc tgagcggtgc cggtcatcgg ccagcgtttt
     5401 cagccggtcc ttaagttctt catcgagcct gatggatgtt gctgatgaca taaaagcccc
     5461 tttgtagtca tatgtattac acatgactac tttaatccgt ggcggcgcta tttgccagaa
     5521 atacggcttt tgctgcccat ttttcagctt cgcttccact aatcagggct gctacaggct
     5581 cactcatcat ccaggactat tgcagcctgc gggttaaagg acctgttact ggcggcaacc
     5641 atcatcattg agcttttggg tggtgactgg tcgcggatcc tgtaagccgt catttttcag
     5701 actgaattca aaatttataa cctgtcgcat tgccgggtgc cctgcgggat atagccagtg
     5761 cctcattgcc gtcagcgttt cccgaaaaaa aaggtgacag ccggtaagcg acacgttgtc
     5821 cactcgtcca tcgtccagaa tatcaaacgt ggcggtcacc tgaccttcga tgcgttttgc
     5881 tgcggcctgt accgggtagg ttggctgcag tgtggttacg ggttttagtg gtatcgcatg
     5941 taccggagtg aggaacatgg ccgccagcac tgtcagagta aaagctttca tggtctgaaa
     6001 tccagagggg cgtttttgaa tatttgccag gtattttcat ccggtgccgg cggaattttg
     6061 gcgtgggtaa cggctgatat tgctgcttta cagagtttcg gatctcctcc ttcagcaatg
     6121 gcgctgttca gagtgccatc acgttgtatg gatatccgca cggtgcacat ttttcccgca
     6181 taggcctctg gttcaaacat ctgcatttca acagcggtct tgatggccgc agcgtagcta
     6241 ctgattttag atgacttatc cagagtataa attttcggcg gctgggtggt ggaatgctta
     6301 caggccgtca ggagtaaggg gctgcacaat gccagcagga taaagggtat taaacgcatc
     6361 gggttgatga cctgtcagta aaaaatgtta catgtatatt tgtcactctt tacggccacg
     6421 ttcatttaca taaacacggc ttatcgtatc gtttttacac caaacggccc gccatgcagt
     6481 tgcgtgcata ggcatgcagc gggttacaat ttaccgggcg cgtttccggc ggttttccgg
     6541 gtggtttgtt gcctgtttta tcccgtagcc gccggaaacg ccctcagacc gtctgagcgg
     6601 tgcgggcaat gcgacgttat ggtaaatacc ccttaccggc ggcgctgtgg ctgccaggtg
     6661 aaggtggttt ccgtagcctc gtatttctcc cgacgcattt cctgcgcccg cctggcttca
     6721 cgttcgtcct gaatggcacg aagcaccctt tcccgcacct gttgttttac cgcctttggc
     6781 agcgttttgt tccctttcag gcccatggcc agtgcctgag cgacaaaggc gtccgccagg
     6841 cgcttttctt cagcgggagt cagtttcagg tgcgtcatag tggcctccgg gcgtctcagg
     6901 tttcccgaac agtttaaccg caggtcctct cctcatgcca gttagctttc agatgtgtct
     6961 ataccatctg aaatgtaagt gtgatgaatg tcttaaataa taaaatttgt tgccatgaga
     7021 aattttcggg aaaagacgcg atctgcttac aggtggctcc gggcgctttt ttgtgcctga
     7081 agatgagaac taatctcata tattgacacc tgaaaacggg aggagtaagg taataattat
     7141 actgtgtata tgtacagtgt gcgccgggag accggtagag atcaaggggt gaaagtcccc
     7201 gaccattgaa ggaccagcaa tccacaaggt ccccgagtca tgcgttgcat accgcgaggt
     7261 atggggcgaa gcgttgacag gggtgttgac aggccagcca ttgagccacg aaatgtatat
     7321 taaattaccg ggtgccgacg ttgtactgtt aacggaaggc aacatcatag ggtgcgatac
     7381 tgcgagtgcc acatggaccc ggcggggtct gagaccctgg catgtcaata cgatctctac
     7441 gcgggaaccg ggagatctcc cctctgacca tctgccagtg tcggagatgg cccgcaccgg
     7501 gaagacgagg agtcatagcc ggtgatgtac ggagaggaga agtcggactc gctcatagta
     7561 gcggcgaagc aggcgaacaa cccgaaagga gcggagtcag tggagcgaag gagcggggcc
     7621 aaggggaacg cggaacagcc acacatgcgc cggacacaga gccgggaaag catgtcacag
     7681 aggctgtcac gcgtgcggga agctgcgaag cagcggaaga aagaacggtt tacagcattg
     7741 ttccacctgc tgacagtcga agcactggaa gccgcattcc tctccctgag caggaaagcg
     7801 gccgccggag tggatggcat caggtggatg gactacgccg gaaacatgaa gaacaacata
     7861 acagatctgc accggaggct acatcagggc agctacaggg cgcagcccgg caggcgtcac
     7921 tacatcccaa aagcggatgg aaaacaacgc ccgctcggca tcgcctcgct ggaggacaag
     7981 atcgtccagt atgcgctggt gaaaatcctg aacgcagtct atgaaaacga ctttatgggg
     8041 ttctcatacg ggttcagacc cgggcgaagc cagcacgatg cactggacgc actggccaca
     8101 gggctggtac gcactaacgt aaactgggta ctggatgccg acatcagtca gttcttcgac
     8161 agggtgagcc acgaatggct gatcaggttc acagagcatc ggatcggcga ccggagggta
     8221 atcaggctca tacgtaagtg gctcacagcc gggacgtcgg aggagggtca atggcgagca
     8281 acggaggaag gcaccccaca gggtgcggtc atctcaccgc tgctggcaaa catatacctc
     8341 cactacgtat tcgatctgtg ggcgcatcag tggcgacgtc gctatgccac aggcaatgtg
     8401 gtaatggtca gatacgccga tgacatcgtc atcgggttcg acaaacgata cgatgcccgg
     8461 cgcttccgta tagccatgca gcgcagactg agggagttcg gactcacggt tcacccggag
     8521 aaaacccgtc tgatggagtt cggccgcttc gctgccgaaa accgtgccat caggggaaaa
     8581 ggcaaaccag aaacgttcaa cttcctcggg ttcacgcaca tcagcgggaa agatcgcaac
     8641 ggcaggttca tgctgatacg aaagacccgc cgggatcgga tgacggcaac tctgaaagcc
     8701 atcaaagacg ggctgcgaag gcgctggcat tactcaatcc ccgaacaggg aaaatggctc
     8761 aggagagtgg ttcagggata cctgaactat cactcggtac cgggcaactt ccccaccatg
     8821 cagaagttca ggacacacgt aacaaacctc tggcgccggg cgctcaggcg caggagccag
     8881 aaggatgata cgacctggac gaaagcaaac aaactggcag ccgcatggct accaagggtt
     8941 cgggttcttc atccatggcc tgtggagcgg ttcaccgcca gacacccgag gcaggagccc
     9001 ggtgcgtaaa tcgcgcacgc cgggatctgt gcggggggta tccggtaacg ggtatcccta
     9061 ccgcgacatg ttggctttaa tttattgttt taaatgtcaa agaggaaaac ggtatgagtg
     9121 gtggagacgg tcgaggtccg ggtaattcag gtctgggaca taatggtggt caggccagtg
     9181 ggaatgtgaa cggtacgtct ggtaaaggtg gcccttcatc aggtggtggt acggatccaa
     9241 acagcgggcc gggctggggt acgacgcata cgccgaatgg agatattcat aactataatc
     9301 cgggggagtt tggtaacggc gggagtaaac ccggtggtaa tggtggtaac agcggcaatc
     9361 atagcggtag ctctggtggc ggacagtctt cggccactgc gatggccttc ggtctgcctg
     9421 ccctggctac tccgggggct gaaggactgg ctttatcagt ttccggcgat gcgttgtcgg
     9481 ccgccgttgc tgatgtgctg gctgccctga aggggccgtt taagtttggt ctgtggggga
     9541 ttgcgatcta cggtgtgctg ccttctgaga ttgcaaaaga tgatccgaaa atgatgtcaa
     9601 aaattatgac gtcattaccg gccgatgcgg tgacggagac tccggcaagt actttaccac
     9661 tggaccaggc gacggttcgt gtcagacaac gggttgtgga tgtggtgaag gatgagcggc
     9721 agcatattgc ggttgtcgca ggtcggccaa tgagtgtccc tgtggtggat gcgaaaccga
     9781 caaaacgtcc gggggtattc agtgtgtcga ttccgggtct cccgtctctg caggtgagcg
     9841 taccgaaagg tgttccgaca gcgaaagccc cgccaaaagg cattgttgct gaaaaaggtg
     9901 attcacgtcc ggctggtttt acagccggtg gtaactcccg tgaggccgtt attcgtttcc
     9961 cgaaagagac cggacagaag ccggtttatg tgtcggtgac agatgttctt accccggcac
    10021 aggtaaaaca gcgtcaggag gaagaaaagc gtcgccagca ggcatgggac gccgctcatc
    10081 cggaagaggg gctgaaaaga gactatgata aagcgaaagc tgagctggat gccgaagata
    10141 aaaatattgc gaccttaaac agccgcattg catcgacaga gaaggcgctc cccggtgcaa
    10201 gggctgctgt tcaggaagcc gataaaaagg taaaagaggc agaggcaaat aaagatgatt
    10261 ttgtgactta taacccgcct catgaatatg gctccgggtg gcaggatcag gttcgctatc
    10321 ttgataagga tattcagaat cagaatgaga aattaaaagc ggctcaggca tctttaaacg
    10381 caatgaatga atccttatcc agagataagg ctgcacttac cggggcgatg gagagccgga
    10441 aacaaaagga gaaaaaagcg aaggatgcag aaaataagtt aaatgaggaa aagaacaagc
    10501 ctcgcaaggg gactaaagac tacggccatg attatcatcc agtacctaaa acagaagaca
    10561 taaagggact tggagagtta aagcgaggtg atcctaaaac acctaaacaa ggtggtggtg
    10621 gtaagcgggc ccgatggtat ggcgataaaa aacgtaaaat ttatgaatgg gattcccagc
    10681 acggtgagct tgaagggtat cgtgccagtg atggcgaaca cctaggcgca tttgatccca
    10741 aaacgggtaa gcaggttaaa gggccagatc caaaacggaa cattaaaaaa tatctttgag
    10801 aggtaaatat ggggcttaaa ttacgtttga catggtttga taagaaaact gaagagttta
    10861 aaggggaaga gtattctaaa gactttggtg acgatggttc tgtcattgaa agcttgggaa
    10921 tgcctttaaa ggataatatt aataatggtg agtttgattt gaagaaagaa tgggttcctt
    10981 tactacagcc attttttaaa aatcaaataa aacctgatgt ggataattgt tatatagctt
    11041 ttgattatcg ggacggtaac tggtaacaaa aaattatcaa ggatgatttg aaatactgac
    11101 ttccggtgtt cagggatgaa tgccggagtc tttaaataca gggaaagtat atgaaaaagg
    11161 taaagactat ttttcttttt atattgattg ttgctggctt tcttctggcc gcatgtcagg
    11221 caaattatat ccgggatgtt cagggcggaa cggtggcacc atcgtcctcc tctgaactga
    11281 cggggatcgc ggttcagtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
    11341 cgtaatcttt tgccctgtaa acgaaaaaac cacctgggga ggtggtttga tcgaaggtta
    11401 agtcagttgg ggaactgctt aaccgtggta actggctttc gcagagcaca gcaaccaaat
    11461 ctgtccttcc agtgtagccg gactttggcg cacacttcaa gagcaaccgc gtgtttagct
    11521 aaacaaatcc tctgcgaact cccagttacc aatggctgct gccagtggcg ttttgcgtgc
    11581 tttcccgggt tggactcaag tgaacagtta ccggataagg cgcagcagtc gggctgaacg
    11641 gggagttctt gcttacagcc cagcttggag cgaacgacct acaccgagcc gagataccag
    11701 tgtgtgagct atgagaaagc gccacacttc ccgcaaggga gaacggcgga caggtatccg
    11761 gtaaacggca gggtcggaac aggagagcgc aagagggagc gacctaccgg aaacggtggg
    11821 gatctttaag tcctgtcggg tttcgcccgt actgtcagat tcatggttga gcctcacggc
    11881 tcccacagat gcaccgaaaa agcgtctgtt tatgtgaact ctggcaggag ggcggagcct
    11941 atggaaaaac gccaccggcg cggccctgc
//
