LOCUS       NZ_CP082027            10049 bp    DNA     circular CON 10-JAN-2024
DEFINITION  Klebsiella pneumoniae strain KP18-2073 plasmid pKP18-2073-3,
            complete sequence.
ACCESSION   NZ_CP082027
VERSION     NZ_CP082027.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN20928635
            Assembly: GCF_019823935.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 10049)
  AUTHORS   Qin,S. and Dong,H.
  TITLE     Klebsiella pneumoniae strains
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10049)
  AUTHORS   Qin,S. and Dong,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2021) Institute of Drug Discovery & Development,
            Zhengzhou University, 100 Science Avenue, Zhengzhou City, Zhengzhou,
            Henan 450001, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: unicycler v. v.0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 12x
            Sequencing Technology             :: Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/10/2024 03:40:39
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,047
            CDSs (total)                      :: 5,925
            Genes (coding)                    :: 5,706
            CDSs (with protein)               :: 5,706
            Genes (RNA)                       :: 122
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 77 of 219
            Pseudo Genes (incomplete)         :: 157 of 219
            Pseudo Genes (internal stop)      :: 29 of 219
            Pseudo Genes (multiple problems)  :: 40 of 219
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP082027.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..10049
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="KP18-2073"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pKP18-2073-3"
                     /geo_loc_name="China"
                     /lat_lon="34.73 N 113.70 E"
                     /collection_date="2018-11-15"
                     /collected_by="Zhengzhou University"
     gene            join(8746..10049,1..382)
                     /locus_tag="K6166_RS29925"
     CDS             join(8746..10049,1..382)
                     /locus_tag="K6166_RS29925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase domain-containing
                     protein"
                     /protein_id="WP_032440457.1"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSVSGDALSAAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPKMMSK
                     IMTSLPADAVTETPASTLPLDQATVRVRQRVVDVVKDERQHIAVVAGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPSLQVSVPKGVPTAKAPPKGIVAEKGDSRPAGFTAGGNSREAVIR
                     FPKETGQKPVYVSVTDVLTPAQVKQRQEEEKRRQQAWDAAHPEEGLKRDYDKAKAELDA
                     EDKNIATLNSRIASTEKALPGARAAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNEKLKAAQASLNAMNESLSRDKAALTGAMESRKQKEKKAKDAENKLNE
                     EKNKPRKGTKDYGHDYHPVPKTEDIKGLGELKRGDPKTPKQGGGGKRARWYGDKKRKIY
                     EWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPKRNIKKYL"
                     /name="colicin-like bacteriocin tRNase domain-containing
                     protein"
     gene            392..649
                     /locus_tag="K6166_RS29930"
     CDS             392..649
                     /locus_tag="K6166_RS29930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /GO_function="GO:0015643 - toxic substance binding
                     [Evidence IEA]"
                     /GO_process="GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E3-like toxin immunity protein"
                     /protein_id="WP_032440455.1"
                     /translation="MGLKLRLTWFDKKTEEFKGEEYSKDFGDDGSVIESLGMPLKDNIN
                     NGEFDLKKEWVPLLQPFFKNQIKPDVDNCYIAFDYRDGNW"
                     /name="colicin E3-like toxin immunity protein"
     gene            734..883
                     /locus_tag="K6166_RS29935"
     CDS             734..883
                     /locus_tag="K6166_RS29935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_032440454.1"
                     /translation="MKKVKTIFLFILIVAGFLLAACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     gene            complement(1066..1224)
                     /locus_tag="K6166_RS30545"
     CDS             complement(1066..1224)
                     /locus_tag="K6166_RS30545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071596352.1"
                     /translation="MHEPPVQPDCCALSGNYHLESNPDGHAKTPLAAAIGNWIYTREIL
                     RGLEVGA"
                     /name="replication initiation protein"
     gene            2356..4320
                     /locus_tag="K6166_RS29940"
     CDS             2356..4320
                     /locus_tag="K6166_RS29940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_071177727.1"
                     /translation="MFNTDSLAWQWHIPELARQIYPWVSWLYREYYSLAGFFLVIGLFF
                     GLAELWAGRMRFVGLSHKARREYADRYKRDNNRTVPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLV
                     AGVLLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSTEQARKQQAVFLGKDEQGQPVLVPRDTWRKTNIQILGLPGSGKSAMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLV
                     AGFNLSETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     KLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFRRWPHASIMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPADFV
                     KTTVLDNTPIRWFYRAASQESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARLAFSSPLRVERREISLKTFPVLAKTDPLAEY
                     LPPADGGGPKADDDFAGLY"
                     /name="TraM recognition domain-containing protein"
     gene            4320..5051
                     /gene="mobC"
                     /locus_tag="K6166_RS29945"
     CDS             4320..5051
                     /gene="mobC"
                     /locus_tag="K6166_RS29945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WP_071177728.1"
                     /translation="MTLEQYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRAKYPVDGRQVSVWGLTPHGVAFSFDEDEPLTDVIPFQP
                     SRVSAAQLPHRLAVQSLRLSMEGRGATGWRYLHRMALKGMKVPDALAELDGRTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRRANGIDEIWYICPDRPTLVRVQRAILAVDEIVNPQTGEA
                     RKTVELDRERLFACFKFMTTE"
                     /name="mobC"
     gene            5058..5588
                     /locus_tag="K6166_RS29950"
     CDS             5058..5588
                     /locus_tag="K6166_RS29950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_071177729.1"
                     /translation="MALYMVFIEDVEDKDGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGENPQWERDKPTGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQINAMIEVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(5615..5794)
                     /locus_tag="K6166_RS29955"
     CDS             complement(5615..5794)
                     /locus_tag="K6166_RS29955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165970.1"
                     /translation="MTKQEKTALNMAKFIKDQSLLLLEKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(5820..6248)
                     /locus_tag="K6166_RS29960"
     CDS             complement(5820..6248)
                     /locus_tag="K6166_RS29960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001140599.1"
                     /translation="MAKTLLNLSQAAQAAGITRRTLYNHVKQGKVTVSRDGKNNPVVDV
                     SELIRVYGNVNIPEKQIPGISHRENTQKNFPQEQLLAMQKELADLRQAVTLMLEDKTSR
                     EEERRQHDDERRKLQAEVDRLTTELTQKKKRFWSGWFS"
                     /name="hypothetical protein"
     gene            complement(6412..6726)
                     /locus_tag="K6166_RS29965"
     CDS             complement(6412..6726)
                     /locus_tag="K6166_RS29965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001136729.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]; GO:0009432 - SOS response [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_071177730.1"
                     /translation="MPQVTISALAQRDLQRLQDFLKTKNRLAARKGGEVIVRAIQQLKT
                     LPDIGRPVPFLPLEYKELVIGFGDSGYVMLYRHDREMDQIVIVTVRHQKESGYPGADSL
                     "
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(6714..6992)
                     /locus_tag="K6166_RS29970"
     CDS             complement(6714..6992)
                     /locus_tag="K6166_RS29970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014542884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family ribbon-helix-helix protein"
                     /protein_id="WP_000093040.1"
                     /translation="MSSATSIRLDEELKDRLKTLADDRHRSAHALMLEAITEYIDREEK
                     RSQYLRDGQAAWQHYQETGLHLTAEEAEAWISTWGTENEQDAPPCHR"
                     /name="CopG family ribbon-helix-helix protein"
     gene            complement(7167..7532)
                     /locus_tag="K6166_RS30290"
     CDS             complement(7167..7532)
                     /locus_tag="K6166_RS30290"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /GO_function="GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="WP_072354022.1"
                     /translation="MKAFTLTVLAAMFLTPVHAIPLKPVTTLQPTYPVQAAAKRIEGQV
                     TATFDILDDGRVDNVSLTGCHLFFRETLTAMRHWLYPAGHPAMRQVINFEFSLKNDGLQ
                     DPRPVTTQKLNDDGCRQ"
                     /name="TonB family protein"
     gene            complement(7529..7900)
                     /gene="tolA"
                     /locus_tag="K6166_RS29980"
     CDS             complement(7529..7900)
                     /gene="tolA"
                     /locus_tag="K6166_RS29980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237044.1"
                     /GO_component="GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA]"
                     /GO_function="GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="WP_001237044.1"
                     /translation="MRLIPFILLALCSPLLLTACKHSTTQPPKIYTLDKSSKISSYAAA
                     IKTAVEMQMFEPEAYAGKMCTVRISIQRDGTLNSAIAEGGDPKLCKAAISAVTHAKIPP
                     APDENTWQIFKNAPLDFRP"
                     /name="tolA"
     gene            complement(8174..8419)
                     /locus_tag="K6166_RS29985"
     CDS             complement(8174..8419)
                     /locus_tag="K6166_RS29985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032440458.1"
                     /translation="MTHLKLTPAEEKRLADAFVAQALAMGLKGNKTLPKAVKQQVRERV
                     LRAIQDEREARRAQEMRREKYEATETTFTWQPQRRR"
                     /name="hypothetical protein"
     BGC             3746..10049
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          complement(987..1554)
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="88.795"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          4321..5048
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.416"
                     /coverage="100.0"
                     /evalue="1.34e-140"
ORIGIN
        1 taccggggcg atggagagcc ggaaacaaaa ggagaaaaaa gcgaaggatg cagaaaataa
       61 gttaaatgag gaaaagaaca agcctcgcaa ggggactaaa gactacggcc atgattatca
      121 tccagtacct aaaacagaag acataaaggg acttggagag ttaaagcgag gtgatcctaa
      181 aacacctaaa caaggtggtg gtggtaagcg ggcccgatgg tatggcgata aaaaacgtaa
      241 aatttatgaa tgggattccc agcacggtga gcttgaaggg tatcgtgcca gtgatggcga
      301 acacctaggc gcatttgatc ccaaaacggg taagcaggtt aaagggccag atccaaaacg
      361 gaacattaaa aaatatcttt gagaggtaaa tatggggctt aaattacgtt tgacatggtt
      421 tgataagaaa actgaagagt ttaaagggga agagtattct aaagactttg gtgacgatgg
      481 ttctgtcatt gaaagcttgg gaatgccttt aaaggataat attaataatg gtgagtttga
      541 tttgaagaaa gaatgggttc ctttactaca gccatttttt aaaaatcaaa taaaacctga
      601 tgtggataat tgttatatag cttttgatta tcgggacggt aactggtaac aaaaaattat
      661 caaggatgat ttgaaatact gacttccggt gttcagggat gaatgccgga gtctttaaat
      721 acagggaaag tatatgaaaa aggtaaagac tatttttctt tttatattga ttgttgctgg
      781 ctttcttctg gccgcatgtc aggcaaatta tatccgggat gttcagggcg gaacggtggc
      841 accatcgtcc tcctctgaac tgacggggat cgcggttcag tagaaaagat caaaggatct
      901 tcttgagatc ctttttttct gcgcgtaatc tcttgcactg taaacgaaaa aaccgcctgg
      961 ggaggcggtt tgatcgaagg ttaagtcagt tggggaactg cttaaccggg taactgggta
     1021 tacgagaccg cagataccaa aactgttctt tcagtgtagc cgcagttagg cacccacttc
     1081 aagacctctc aatatctctc tcgtatatat ccagttacca atggctgctg ccagtggcgt
     1141 ttttgcgtgt ccgtccgggt tggactcaag atgatagtta ccggaaaggg cgcagcagtc
     1201 gggctgaacg gggggttcgt gcatacagcc cagcttggag cgaacgacct aaaccgagcc
     1261 gagataccag cagcgtgagc tatgagaaag cgccacgctt cccgaaaggg aaaaaggcgg
     1321 aacaggtatc cggtaaacgg cagggtcgga acaggagagc gcaagaggga gcgacctacc
     1381 ggaaacggtg gggatcttta agtcctgtcg ggtttcgccc gtactgtcag attcatggtt
     1441 gagcctcacg gctcccacag atgcaccgaa aaagcgtctg tttatgtgaa ctctggcagg
     1501 agggcggagc ctatggaaaa acgccaccgg cgcggccctg ccgttttgcc tcacatgtta
     1561 gtcccctgct tatccacgga atctgtgggt aactttgtat gtgtccgcag cgcccgccgc
     1621 agtctcacgc ccggagcgta gcgaccgagt gagcgaggag gcgcaatttc cccggcacct
     1681 gcaagacggt caccggacgc gctgatgtgg gatattgtcc cctgattaag tctttctatt
     1741 ccctgacgat caattatcag gataggtgaa ccatgtcggt ttttcgctga acattttacg
     1801 ttaacctatc tctgttgctt ttttctcctt gcggagaaat gcaagtcatc cccgctgggg
     1861 cgtggctgac ttcaggggaa aagaccttgc ttacgctggc tggacgccac cgacgcacgg
     1921 tcttttcccc tctttctggc tctgtccggc tggacgccgg ccacgccaga aattcacccg
     1981 gcccccgtca ggctcacgcc tgaccaccca caaaaatcca cggtgctgga cgcacctgac
     2041 gaagcagcaa agctgctcct gattttgcgg gtggtcagcc gctcgctatg gcgaactctg
     2101 acgggggccg gaacaccgga aaagaccgca ctggggtgcc gccttttcct ctcacacgct
     2161 ggggcgtgct cgttgttcct ccggggctgc gccttttccg catggggcgg aaagaggctg
     2221 gcctccgcgg tgaaagctga acgcaatcac cacggacgtg accgccgccg ccatgccttg
     2281 gggcatgtcc gcggcttgtg cagcgggggc tgcgcatcac gccggtaaac ccgtcgtctg
     2341 aaggagagag attgcatgtt taatacggat tcgcttgcct ggcagtggca tatcccggag
     2401 ctggcccggc agatttaccc gtgggtcagc tggctgtacc gggagtatta cagtctggcc
     2461 ggtttttttc tggttatcgg cctgtttttc ggtctggctg aactctgggc cggccgaatg
     2521 cgttttgtgg ggctgagcca taaggcgcgg cgggagtatg ccgatcgcta taagcgcgat
     2581 aataaccgga ctgtgccatt ttcggtgctg gcctcgctgg attcacccgg gcggatggtc
     2641 agggcacggc gacatgtctg gaactacgcg tactggctga ccggcgctgc cggtctggcc
     2701 cttctggggg ggctggttgt cgccgtgttt atcgagagct ggggagaaac cgtcctgcgc
     2761 ccggacctct atgcgaacgc agattattac ctgctgcagc cggctaacct gttcgggata
     2821 gcctggtgtg ccggagggct tgtcgccggc gtccttctgg cgcggctgct ggcgttcctg
     2881 ctgcttgatg gtcattttgc ggccgctgac gaggcggtga acgcgaaact gaatcaggag
     2941 agccggcgca gcagccagcg gaccggagaa atgacagacg tccgccacct gcatttcggg
     3001 gagccggtac cggttaacgc gctggccgat ttttccacgg agcaggcccg gaaacagcag
     3061 gcggtgtttc tgggtaagga tgagcagggc cagccggtgc tggtccctcg tgatacctgg
     3121 cgcaagacca atattcagat cctcggcctg cccgggagcg gcaagagcgc gatggccacc
     3181 aacgcgctca ttcgctgcgt acgcgatttc ggggacgctg tggtgtactt cgaccccaaa
     3241 ggggacgcgt gggcgccgca cgtcttccgg gcgcactgcc cggacttcac cctgctggat
     3301 ctgcggccgg ggaaaccggc gcagctgaat ctgttccgcg atctcgacca gtacgccctg
     3361 aagaatctgc tggttgccgg gtttaacctg agtgagaccg gcgacgtggc ggaccactac
     3421 cgcatcagcg agcagaaagc ggcgaagctg attgcggagc agttcccgca gggggcaaac
     3481 attcagcagg tgctggcggc ggcgtacgcg ttgccggagg ccctgaaaaa ggacgtgaag
     3541 gggctgatca ccaagctgga gaacgtggcc gacctgagcg tcctgcagac ggacagcggt
     3601 atcgatgtgg cggggataat caacggcggc ggctgcctgt acgtcatcgg ctcgatggac
     3661 gatgaggcgg tgatccgggt gcagaagatg ctgtttgccc gctgcgcgca aatcatcatt
     3721 gcgcgggatg agttccggag gtggccgcac gcgagcatca tgctcgatga gattaaatat
     3781 ctgctgtcga agtacgtgct gaacgcgctg gggacgctgc gcagccggga ctgcaacctg
     3841 ctgctcgcgc accagtcgct gggcgacttc ggtcagtgcg ggcaggatct gcccgctgat
     3901 tttgtgaaaa ccacggtact ggataacacg ccgatccgct ggttctatcg cgcggccagc
     3961 caggagtccg cgcagtgggc ggcgggccag accggggaga tccgggtgga cgtggagcgc
     4021 cgccgggcca gccgggaggc ggggaacgtg gagcacatca gcggcgacag cttcatccag
     4081 aaggaggcgc ggccgctgtt tgacgtgaac accctgcaac acctgcctga cgggttcgcg
     4141 gtgatgaccg ggctgggcgt ggcgcggctg gcgttcagca gcccgctgcg ggttgagcgc
     4201 cgggaaattt cgctgaaaac cttcccggtg ctggcgaaaa cggacccgct ggcggagtac
     4261 ctgcctccgg ccgacggcgg cggaccaaaa gcggatgatg attttgcggg gctttactga
     4321 tgacactgga gcagtacaac gtcagccacg cgaagcggca ggcgcggaac gcggagaaaa
     4381 cacggctgac cctgcgctgg ctgcgggaag agctgtgttc cacggcggag ctggttgcgc
     4441 gacggctggg gattgcggcg gtacagccgg tttaccgctt cctggacagt ctggtggcga
     4501 aagggctgct ggtccgggcg aaatacccgg ttgacggccg tcaggtcagc gtgtgggggc
     4561 tgacgccgca cggggtggcg ttctcgttcg acgaggacga gccgctgacg gacgttattc
     4621 cgtttcagcc gtcaagggtg tcggcggcgc agctgccgca ccggctggcc gtgcagtccc
     4681 tgcgcctctc catggagggc cggggggcca ccggctggcg gtacctgcac cgtatggccc
     4741 tgaaagggat gaaagtgccg gacgcgctgg cggagctgga cggcagaacg gtggcatttg
     4801 aggtagagcg caccgtcaag agccgcaggc ggtatcagga agtggtgtcc gggtatctgt
     4861 tcaaccgccg ggcgaacggc atcgatgaaa tctggtacat ctgccctgac cggccgacgc
     4921 ttgtccgggt acagcgggcg attctggcgg tggatgaaat cgtgaatcca cagaccggcg
     4981 aggcccgcaa aacggtggag ctggaccggg agaggctgtt cgcgtgcttt aaatttatga
     5041 caacggagtg agggattatg gcgctgtaca tggtatttat tgaggacgtg gaagacaaag
     5101 acggcgagcg ctgcattatg ccaaccagcg agcatctgga agacatggtg gtgctcgcct
     5161 ggcagaccgg ggcgtatgtt ttctgttttg tcgaccgggc ccagcatctg gcgatgatga
     5221 aatccatcgg gatgtatgac gagcggctca acagcgacga aaaagccacg tactttttca
     5281 ccggtgaaaa tccgcaatgg gaacgggaca aacccacagg aatgaagctg tgtgattacc
     5341 tctatcgaaa ggtggagcgg ttcgccatcg agaacggtgt gcaggtggtt gatgccggga
     5401 agtgctcacc ggagcagatt aacgcgatga ttgaggtggt cagcgagcgg cacggggaaa
     5461 gcgcggtgaa cgagaagctg aaagcggagc tgatgcggga atataacagc cgctatttta
     5521 acgtgccggt gcctgaactg ccggaggaca aacccgcccg ccggtggtgg ccgttcggca
     5581 aaaaataacg ccagcaccgg catggccggt gctgttattc ttccccgagc ttatcccgca
     5641 tcacctgcca gagtgtttcc gcatcctcgt gaagccgttc gcacatgtcg gccggggtat
     5701 ccatgtccag ctcgttcagc ttttccagca acagcagcga ctgatcctta atgaatttag
     5761 ccatattgag ggcagttttt tcctgcttcg tcatgttctc cattcccttc ggtaagttct
     5821 cagctaaacc agccggacca gaagcgtttt ttcttctgtg tcagttctgt tgtcaatctg
     5881 tccacttcgg cctggagttt ccggcgctca tcatcgtgct ggcggcgttc ttcttcccgg
     5941 gatgttttat cttccagcat cagggtgaca gcctgccgga gatcggccag ttccttttgc
     6001 attgccagaa gctgttcctg tgggaaattt ttttgtgtat tttcacggtg tgaaatgccg
     6061 ggaatttgtt tctcagggat attcacattt ccgtatacac ggatcagttc tgacacatcc
     6121 acgacagggt tgtttttccc gtcacgtgac acggtaacct ttccctgttt tacatgattg
     6181 tatagcgttc ttctggtaat gcccgcggct tgtgctgcct gtgaaaggtt gagcaatgtt
     6241 ttcgccactg tatacccctg attttatcgg gtgtgaaagg tgtgtaattt ctcacggtaa
     6301 atgtatcgct gaattttacc cgtttttccg ttcaggatgt ggttttctga cgcgctgcgc
     6361 ttgtcaaacc ggcgacggcc acgcaatgaa ttgcgccccc gtcatcggtt attagaggct
     6421 gtctgccccc ggatacccgg attctttctg atgcctgacg gtgacgatca caatctggtc
     6481 catttcccgg tcgtggcggt agagcatcac atagccactg tcgccaaacc cgatcaccag
     6541 ttccttatat tccagcggca gaaacggcac cgggcggcca atgtctggca atgttttcag
     6601 ttgctggata gcccggacga tcacctcacc tccttttctg gctgccagcc gatttttggt
     6661 tttgagaaag tcctgaaggc gttgtaaatc gcgctgtgcc agcgcggaaa tcgttacctg
     6721 tggcatggcg gagcatcctg ctcgttctca gttccccagg tacttatcca ggcttccgct
     6781 tcttccgcag taaggtgcag acccgtttcc tgatagtgct gccatgcggc ctgtccgtcc
     6841 cgcaggtact ggctgcgctt ctcttcccgg tcgatgtatt cggtgattgc ctccagcatt
     6901 agcgcgtgcg ctgagcggtg ccggtcatcg gccagcgttt tcagccggtc cttaagttct
     6961 tcatcgagcc tgatggatgt tgctgatgac ataaaagccc ctttgtagtc atatgtatta
     7021 cacatgacta ctttaatccg tggcggcgct atttgccaga aatacggctt ttgctgccca
     7081 tttttcagct tcgcttccac taatcagggc tgctacaggc tcactcatca tccaggacta
     7141 ttgcagcctg cgggttaaag gacctgttac tggcggcaac catcatcatt gagcttttgg
     7201 gtggtgactg gtcgcggatc ctgtaagccg tcatttttca gactgaattc aaaatttata
     7261 acctgtcgca ttgccgggtg ccctgcggga tatagccagt gcctcattgc cgtcagcgtt
     7321 tcccgaaaaa aaaggtgaca gccggtaagc gacacgttgt ccactcgtcc atcgtccaga
     7381 atatcaaacg tggcggtcac ctgaccttcg atgcgttttg ctgcggcctg taccgggtag
     7441 gttggctgca gtgtggttac gggttttagt ggtatcgcat gtaccggagt gaggaacatg
     7501 gccgccagca ctgtcagagt aaaagctttc atggtctgaa atccagaggg gcgtttttga
     7561 atatttgcca ggtattttca tccggtgccg gcggaatttt ggcgtgggta acggctgata
     7621 ttgctgcttt acagagtttc ggatctcctc cttcagcaat ggcgctgttc agagtgccat
     7681 cacgttgtat ggatatccgc acggtgcaca tttttcccgc ataggcctct ggttcaaaca
     7741 tctgcatttc aacagcggtc ttgatggccg cagcgtagct actgatttta gatgacttat
     7801 ccagagtata aattttcggc ggctgggtgg tggaatgctt acaggccgtc aggagtaagg
     7861 ggctgcacaa tgccagcagg ataaagggta ttaaacgcat cgggttgatg acctgtcagt
     7921 aaaaaatgtt acatgtatat ttgtcactct ttacggccac gttcatttac ataaacacgg
     7981 cttatcgtat cgtttttaca ccaaacggcc cgccatgcag ttgcgtgcat aggcatgcag
     8041 cgggttacaa tttaccgggc gcgtttccgg cggttttccg ggtggtttgt tgcctgtttt
     8101 atcccgtagc cgccggaaac gccctcagac cgtctgagcg gtgcgggcaa tgcgacgtta
     8161 tggtaaatac cccttaccgg cggcgctgtg gctgccaggt gaaggtggtt tccgtagcct
     8221 cgtatttctc ccgacgcatt tcctgcgccc gcctggcttc acgttcgtcc tgaatggcac
     8281 gaagcaccct ttcccgcacc tgttgtttta ccgcctttgg cagcgttttg ttccctttca
     8341 ggcccatggc cagtgcctga gcgacaaagg cgtccgccag gcgcttttct tcagcgggag
     8401 tcagtttcag gtgcgtcata gtggcctccg ggcgtctcag gtttcccgaa cagtttaacc
     8461 gcaggtcctc tcctcatgcc agttagcttt cagatgtgtc tataccatct gaaatgtaag
     8521 tgtgatgaat gtcttaaata ataaaatttg ttgccatgag aaattttcgg gaaaagacgc
     8581 gatctgctta caggtggctc cgggcgcttt tttgtgcctg aagatgagaa ctaatctcat
     8641 atattgacac ctgaaaacgg gaggagtaag gtaataatta tactgtgtat atgtacagta
     8701 tgttggcttt aatttattgt tttaaatgtc aaagaggaaa acggtatgag tggtggagac
     8761 ggtcgaggtc cgggtaattc aggtctggga cataatggtg gtcaggccag tgggaatgtg
     8821 aacggtacgt ctggtaaagg tggcccttca tcaggtggtg gtacggatcc aaacagcggg
     8881 ccgggctggg gtacgacgca tacgccgaat ggagatattc ataactataa tccgggggag
     8941 tttggtaacg gcgggagtaa acccggtggt aatggtggta acagcggcaa tcatagcggt
     9001 agctctggtg gcggacagtc ttcggccact gcgatggcct tcggtctgcc tgccctggct
     9061 actccggggg ctgaaggact ggctttatca gtttccggcg atgcgttgtc ggccgccgtt
     9121 gctgatgtgc tggctgccct gaaggggccg tttaagtttg gtctgtgggg gattgcgatc
     9181 tacggtgtgc tgccttctga gattgcaaaa gatgatccga aaatgatgtc aaaaattatg
     9241 acgtcattac cggccgatgc ggtgacggag actccggcaa gtactttacc actggaccag
     9301 gcgacggttc gtgtcagaca acgggttgtg gatgtggtga aggatgagcg gcagcatatt
     9361 gcggttgtcg caggtcggcc aatgagtgtc cctgtggtgg atgcgaaacc gacaaaacgt
     9421 ccgggggtat tcagtgtgtc gattccgggt ctcccgtctc tgcaggtgag cgtaccgaaa
     9481 ggtgttccga cagcgaaagc cccgccaaaa ggcattgttg ctgaaaaagg tgattcacgt
     9541 ccggctggtt ttacagccgg tggtaactcc cgtgaggccg ttattcgttt cccgaaagag
     9601 accggacaga agccggttta tgtgtcggtg acagatgttc ttaccccggc acaggtaaaa
     9661 cagcgtcagg aggaagaaaa gcgtcgccag caggcatggg acgccgctca tccggaagag
     9721 gggctgaaaa gagactatga taaagcgaaa gctgagctgg atgccgaaga taaaaatatt
     9781 gcgaccttaa acagccgcat tgcatcgaca gagaaggcgc tccccggtgc aagggctgct
     9841 gttcaggaag ccgataaaaa ggtaaaagag gcagaggcaa ataaagatga ttttgtgact
     9901 tataacccgc ctcatgaata tggctccggg tggcaggatc aggttcgcta tcttgataag
     9961 gatattcaga atcagaatga gaaattaaaa gcggctcagg catctttaaa cgcaatgaat
    10021 gaatccttat ccagagataa ggctgcact
//
