LOCUS       NZ_CP082023             5596 bp    DNA     circular CON 10-JAN-2024
DEFINITION  Klebsiella pneumoniae strain KP18-2073 plasmid pKP18-2073-4,
            complete sequence.
ACCESSION   NZ_CP082023
VERSION     NZ_CP082023.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN20928635
            Assembly: GCF_019823935.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 5596)
  AUTHORS   Qin,S. and Dong,H.
  TITLE     Klebsiella pneumoniae strains
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5596)
  AUTHORS   Qin,S. and Dong,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2021) Institute of Drug Discovery & Development,
            Zhengzhou University, 100 Science Avenue, Zhengzhou City, Zhengzhou,
            Henan 450001, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: unicycler v. v.0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 12x
            Sequencing Technology             :: Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/10/2024 03:40:39
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,047
            CDSs (total)                      :: 5,925
            Genes (coding)                    :: 5,706
            CDSs (with protein)               :: 5,706
            Genes (RNA)                       :: 122
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 77 of 219
            Pseudo Genes (incomplete)         :: 157 of 219
            Pseudo Genes (internal stop)      :: 29 of 219
            Pseudo Genes (multiple problems)  :: 40 of 219
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP082023.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5596
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="KP18-2073"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pKP18-2073-4"
                     /geo_loc_name="China"
                     /lat_lon="34.73 N 113.70 E"
                     /collection_date="2018-11-15"
                     /collected_by="Zhengzhou University"
     gene            join(5388..5596,1..142)
                     /locus_tag="K6166_RS00005"
     CDS             join(5388..5596,1..142)
                     /locus_tag="K6166_RS00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004197756.1"
                     /translation="MYWLIAFLAFIFPGRSFADGYTAYYRCNGTQEASLTVKDNKLSMV
                     VGLSKFNRQLDTYRFKEKGIEVVMQRAASDDNSEAIMSFIMPDVNNMTLLVKNNGYIIV
                     KTTCWLTKTDKQ"
                     /name="hypothetical protein"
     gene            complement(212..478)
                     /locus_tag="K6166_RS00010"
     CDS             complement(212..478)
                     /locus_tag="K6166_RS00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262326.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_004197762.1"
                     /translation="MVWTINYSDRALKSLRKMDKQNARRIVDFMSLRIAVAADPRQSGK
                     PLKGELGEFWRYRVGDYRVLCEIRDDELVILAATIGHRREVYD"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(462..686)
                     /locus_tag="K6166_RS00015"
     CDS             complement(462..686)
                     /locus_tag="K6166_RS00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6290 family protein"
                     /protein_id="WP_000879771.1"
                     /translation="MLAIRLSDEIESRLDSLAKQTGRTKTFYAREAILAHLEDLEDYYL
                     SAETAARVRRGDEAVHSSEDVRKSLGLDD"
                     /name="DUF6290 family protein"
     gene            849..977
                     /locus_tag="K6166_RS30295"
     CDS             849..977
                     /locus_tag="K6166_RS30295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000523570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000523570.1"
                     /translation="MGLKPGPKRIADSTGEPDKRQRDNKKTPGNTDKLKPSKSSKK"
                     /name="hypothetical protein"
     gene            complement(1353..1565)
                     /locus_tag="K6166_RS00020"
     CDS             complement(1353..1565)
                     /locus_tag="K6166_RS00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_021527181.1"
                     /translation="MTELEKQLLSALEQLQQDYSKRLDEWESAFGEWRKMSGLMQRENA
                     ALSERVTRLSQQVERLSGQLQRLSR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(1572..2081)
                     /locus_tag="K6166_RS00025"
     CDS             complement(1572..2081)
                     /locus_tag="K6166_RS00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_032433972.1"
                     /translation="MNSLLTLAKDLEQKSKAQQQSTGEMLKAAFSEHEKSVRAELSESG
                     KRISAAIRAHEQGMSAAMQSNRLSVMRMVGRTWLTITMVSGLLFASLSGVLWYQGSLIA
                     SNLAEIDRQNAALSKLNAKTWGVTYLEDSNGRFLVLPKGTAVDRTQSWTVGNGRSKQNA
                     LRLVKE"
                     /name="MbeB family mobilization protein"
     gene            complement(<2116..2820)
                     /locus_tag="K6166_RS30385"
                     /pseudo=""
     CDS             complement(<2116..2820)
                     /locus_tag="K6166_RS30385"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(2759..3103)
                     /locus_tag="K6166_RS00035"
     CDS             complement(2759..3103)
                     /locus_tag="K6166_RS00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006122514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_062955149.1"
                     /translation="MAVKRTRFLGIRVTDGEYQQLLERCNGRQLAVWMRETCLDTRPAR
                     SLRLPSIDPVLLRQLAGMGNNLNQIARKINGGQWSGADRVQVVAALMAIDAELERLRHA
                     VLEKGADDDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            complement(3093..3269)
                     /locus_tag="K6166_RS00040"
     CDS             complement(3093..3269)
                     /locus_tag="K6166_RS00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004197757.1"
                     /translation="MQKPKKKPLVVKKRSWHPAVMANLMRQAQEEDYRREQMAELERSA
                     RSHFADIGGEDGG"
                     /name="hypothetical protein"
     gene            3909..4067
                     /locus_tag="K6166_RS30390"
     CDS             3909..4067
                     /locus_tag="K6166_RS30390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071596352.1"
                     /translation="MHEPPVQPDCCALSGNYHLESNPDGHAKTPLAAAIGNWIYTREIL
                     RGLEVGA"
                     /name="replication initiation protein"
     gene            4425..4751
                     /locus_tag="K6166_RS00045"
     CDS             4425..4751
                     /locus_tag="K6166_RS00045"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_050516389.1"
                     /translation="MRERTNIMLDIGLKQSLQRLSNKTKMSLSDLINQRLSGSLSVEER
                     IGEKPEWMRNLDPYLANHFSNIELPLPSPLKEYQEKMDEIDSIINDMMVLKEYFRAKFY
                     DEIK"
                     /name="hypothetical protein"
     gene            4777..5322
                     /locus_tag="K6166_RS00050"
     CDS             4777..5322
                     /locus_tag="K6166_RS00050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_023317533.1"
                     /translation="MFFKRYLTEKEIYKAALPIVRLQGRVNTSLRFLSKYSDVNKNGLI
                     ALHLNLLHFISYNIHGVESLKITQAGINKAVQQSPHADEIKNIAQAIGRKGPEYIEGLI
                     RNPWGKKNDNPLIFIMSIEESIKETLNSGYMLYRVAPITEGEAAAFMDVLKQLLLFEDN
                     FRLFGRDLVYKIEKLKIR"
                     /name="hypothetical protein"
     Typing          complement(3356..3432)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="91.25"
                     /coverage="98.0"
                     /evalue="1.04e-21"
     Typing          3621..4018
                     /name="rep_cluster_2370"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="89.526"
                     /coverage="91.0"
                     /evalue="3.03e-139"
     Typing          complement(1238..2769)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.804"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 gagagctgct tctgatgata actctgaggc cataatgtct tttattatgc ctgacgttaa
       61 caatatgacc ttattagtta aaaataacgg ctacattatc gttaagacaa cctgctggtt
      121 aactaaaacc gacaaacagt agaacacgta gttagtcgag tatttaccat aatacctgtt
      181 gtcggcatca aaaaagcccg catagcgggc ttcagtcgta aacttcgcgg cgatgtccaa
      241 tcgtggcggc aaggataaca agctcgtcat ctcggatctc acacagaacg cgataatctc
      301 ccacccgata gcgccagaac tcgcccagct cacctttgag cggcttccct gactggcgag
      361 gatcggcagc aactgcaatg cgtaaactca taaaatccac aatccgtcgt gcgttctgtt
      421 tgtccatctt gcgtaacgat ttgagcgccc gatcggaata gttaatcgtc cagaccaagt
      481 gacttcctca cgtcttcaga cgaatgcact gcttcatcac cacggcgaac gcgtgcagca
      541 gtttctgctg aaagataata atcctccagg tcttccagat gcgccagtat tgcttcccgc
      601 gcataaaacg tctttgttct gccggtttgc ttcgccagcg agtccagacg ggactcaatc
      661 tcatcagata accggatagc aagcatataa acctcccgaa caccatttgc tatacatgta
      721 tatcaacagt accacaatca acctaaaaga acaaaaaata atcttgttag cccctcttga
      781 taatccgtaa cgcggtcaat atatctagct tgagggcttt cctcttaact attgaaagga
      841 taacgattat gggtcttaag ccgggtccga aacgaattgc cgattcaaca ggggaacctg
      901 ataaacgcca gcgtgacaat aaaaaaacgc cgggaaatac tgacaaattg aaacccagca
      961 aatcttcaaa aaaataagtc agtagttaaa agccctccgg ggcttttttg tatctgccgt
     1021 tcacgctgat taaacataac acccgttgcc cgcaccgccc cggacgggtt cgccgcgaac
     1081 ggggtggcag tcaggtaaaa ccggcaacaa accacccgat aaaccgcccc gttcgcgaca
     1141 gataaatttt acctggctgc atagccatgc agccaggtga atgacatccg gtttcagcgt
     1201 aaatgcccga tgagaaccct gttttcctgc atttcagccc agcgacggac cgtcatagct
     1261 tcgctgccgg gaacgcgctt ccgtcagttc cttttcgtgc tgccgttgct cgtgcgcccg
     1321 ttctgcggcc acggccatct catgacgctg cactaccggc tcaaacgctg cagttgcccg
     1381 ctcaagcgtt ccacctgctg actcaagcgc gtcacgcgct cgctcagcgc cgcgttctcc
     1441 cgttgcataa gccctgacat tttccgccat tccccgaagg cgctctccca ctcgtccagc
     1501 cttttcgagt agtcctgctg tagctgctct aatgcgctca gcaactgttt ttccagctcg
     1561 gtcatcggca cttactcctt caccagtctc agcgcgttct gcttcgaccg cccgttcccg
     1621 accgtccagc tctgcgtcct gtctacggcc gtccccttcg gcagcaccag aaaacgtccg
     1681 ttgctgtctt ccaggtacgt cacgccccag gtcttcgcgt tcagcttcga cagcgctgcg
     1741 ttctgccggt cgatttcggc cagatttgac gctatcaagc tcccctgata ccacaacacc
     1801 ccgctcaggc tggcgaacag cagtcctgac accatcgtga tggtcagcca tgtccgcccg
     1861 accatacgca tcacgctcag gcggttcgac tgcatggccg cgctcatacc ctgctcgtgg
     1921 gcgcggatgg cggcgctgat tctcttcccg ctttcgctca gttccgctct gacagacttc
     1981 tcgtgctcgc tgaatgcggc tttcagcatt tcgccggtac tctgctgctg cgctttcgat
     2041 ttctgctcta agtccttcgc cagcgttaaa agactgttca tagatggctc ccttaagtcg
     2101 gatgtttcgc cctccgtccg ggtcggcaat gctgatgctg ctctttgtgg tgcgcacgac
     2161 ctcaaaacct gcgctttcca gcgcctcagt gacgtcctga cgcgttttaa gctcccctga
     2221 cgaggcgagg gccagtaagc cccgcgtaat agcctctgcg gcttcctgct tcgttttggg
     2281 taacgaagat ggcgtcacca gcgcgcgccg gttctccggt gcgttcgggt cgtgcagccc
     2341 cagtcgcccg ttcaccacgg tctgccacgc atcgatgcgc ggacggtctg cccggtcgta
     2401 gtacggctgg agacgcctgc cggtcagcag ttccgtgttc ggtaccagaa aattcagttc
     2461 cagccgcccc ttgtcccggt gttcaaccca cagcacgctg tactggtctt tatcgagtcc
     2521 gggcatcaga acccgctcga agctcgccat cagcttttca cgctggccgg gcggtaaatc
     2581 ctgttcagca aaagacagga cgccggaggt gtactttttg gcgtagggcg aggcgtcgat
     2641 aagctcccgg acctcctccg gctttccctg caggacgatg gccccgtcgc gctggcggtc
     2701 tttccccagc agataatcca ccggcccggc accgccgcct cgccctcgcg gatgaaactt
     2761 aacgatcatc gtctgccccc ttttccagca cggcatgccg cagccgctcg agttcggcat
     2821 caatggccat cagcgcggcc accacctgaa cccggtcagc gcctgaccac tggccgccgt
     2881 taattttccg ggcgatctgg ttgaggttgt tccccatccc ggcaagctgg cgcagcaaaa
     2941 ccggatcgat ggacggcagc cgcaacgacc gcgccgggcg cgtatcgaga caggtttccc
     3001 gcatccacac cgccagttgc cttccattgc agcgctccag cagctgctgg tactccccat
     3061 cggtcacccg gatacctaaa aaacgggtac gcttaaccgc catcctctcc tccgatatcc
     3121 gcaaaatgtg accgggctga acgctcaagc tctgccatct gctcccggcg gtaatcttcc
     3181 tcctgtgcct gacgcatcag atttgccatc actgccggat gccaggatct tttttttacg
     3241 acgagcggct tcttcttcgg cttctgcatt atccctcctt cgcaaacgcc ggacggcacg
     3301 gaaaagtttt ccgggtcgct ccggcgttgg acaagcgcca gcgcgtcagt ggtgttagcg
     3361 ggtttcgggg cgcagccctg aaccagtcac agcgtttacg ctgtgtatac tggcttacta
     3421 tgttggcatg agagcacgat tcatgaaagt gcctcatgtg gcaggtgtga tatgtggctc
     3481 aggagcgtcc gcaataccat gcacggacag atgaaatgac gcctcctcgc tcactcggta
     3541 gctgcgctcc tgccgttcgg ctgcggcggg cgtgaccgac tcacggataa cgggataaag
     3601 caaaggcgct caaatcagaa agggccggag aaaaggatgc ggcgttgccg tttttccata
     3661 ggctccgcct cccctgacga gcatcacaga aatggacgct caagtcagag gaggacgaaa
     3721 cccgacagga cttaaagatg ccggtgattt ctctccggcg gctccctcgt gcgctctcct
     3781 gttccgaccc tgccgtttac cggatacctg ttccgccttt ttccctttcg ggaagcgtgg
     3841 cgctttctca tagctcacgc tgctggtatc tcggctcggt ttaggtcgtt cgctccaagc
     3901 tgggctgtat gcacgaaccc cccgttcagc ccgactgctg cgccctttcc ggtaactatc
     3961 atcttgagtc caacccggac ggacacgcaa aaacgccact ggcagcagcc attggtaact
     4021 ggatatatac gagagagata ttgagaggtc ttgaagtggg tgcctaactg cggctacact
     4081 gaaagaacag ttttggtatc tgcggtctcg tatacccagt tacccggtta agcagttccc
     4141 caactgactt aaccttcgat caaaccgcct ccccaggcgg ttttttcgtt tacagtgcaa
     4201 gagattacgc gcagaaaaaa aggatctcaa gaagatcctt taaatgatcg tcagttctgt
     4261 tatccccgga gctccatcca tcccacggaa aaacacccgc cactgatgcc ctcagcgcac
     4321 tgcacatcat gtacaaacca ctccctccct ccttttttat tgcgtttctt aatgcgcaat
     4381 ggtagctttg tgcgcaacaa aaaacgcaat agcaaggggg ggtaatgaga gaaagaacaa
     4441 atatcatgct agatatagga ttaaagcaat ccttacaacg gctttcgaat aaaaccaaaa
     4501 tgagtctaag tgatcttatt aaccagagat tatcaggttc actttcagtt gaagaacgca
     4561 ttggcgaaaa gcctgaatgg atgagaaacc ttgatcccta tctcgccaat cacttttcaa
     4621 atattgagtt accgctccct tcaccattaa aagaatacca agaaaaaatg gatgagatag
     4681 attcaattat taatgacatg atggttctaa aggaatattt cagagctaag ttttacgacg
     4741 aaataaaata ggcacgctat acggaggcgg agaattatgt tttttaaacg gtatttaacg
     4801 gagaaggaaa tatacaaggc agcactccca attgttaggc ttcaagggag agttaacact
     4861 tcattacgtt ttttatccaa atactcagat gtaaacaaaa atggattaat tgcattgcat
     4921 cttaatttat tacattttat ctcatacaac atacacggtg tagagtctct gaaaatcact
     4981 caagcaggca taaacaaagc tgtacaacaa agccctcacg cagatgaaat aaaaaatatt
     5041 gcgcaggcaa taggacgcaa aggacctgag tacatcgaag gattaattag aaacccatgg
     5101 gggaagaaaa atgataatcc actaatattt ataatgagca ttgaagaaag tataaaagaa
     5161 acccttaact caggttatat gttatataga gtagctccca taactgaggg tgaagcagct
     5221 gctttcatgg atgttcttaa acaattatta ttatttgagg ataattttcg cctttttggt
     5281 agagacttgg tttataaaat agaaaaatta aaaatcagat aacaaagtat ggtgaacgaa
     5341 aaatgaaaga gaggatagtg aaatgtgtaa taccaagatt ctcaactatg tattggttaa
     5401 ttgcgttcct agcatttata ttccccggtc gatcttttgc tgacggttac accgcttact
     5461 accgatgtaa tggtactcag gaagcatctc ttactgttaa agataacaaa ctaagtatgg
     5521 ttgtaggtct ttctaagttt aataggcaac tggatactta tagattcaaa gaaaagggaa
     5581 tcgaagtagt aatgca
//
