LOCUS       NZ_CP082022            10049 bp    DNA     circular CON 10-JAN-2024
DEFINITION  Klebsiella pneumoniae strain KP18-2050 plasmid pKP18-2050-3,
            complete sequence.
ACCESSION   NZ_CP082022
VERSION     NZ_CP082022.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN20928633
            Assembly: GCF_019823835.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 10049)
  AUTHORS   Qin,S. and Dong,H.
  TITLE     Klebsiella pneumoniae strains
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10049)
  AUTHORS   Qin,S. and Dong,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2021) Institute of Drug Discovery & Development,
            Zhengzhou University, 100 Science Avenue, Zhengzhou City, Zhengzhou,
            Henan 450001, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: unicycler v. v.0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 12x
            Sequencing Technology             :: Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/10/2024 03:38:20
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,056
            CDSs (total)                      :: 5,934
            Genes (coding)                    :: 5,704
            CDSs (with protein)               :: 5,704
            Genes (RNA)                       :: 122
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 82 of 230
            Pseudo Genes (incomplete)         :: 163 of 230
            Pseudo Genes (internal stop)      :: 29 of 230
            Pseudo Genes (multiple problems)  :: 40 of 230
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP082022.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..10049
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="KP18-2050"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pKP18-2050-3"
                     /geo_loc_name="China"
                     /lat_lon="34.73 N 113.70 E"
                     /collection_date="2018-11-13"
                     /collected_by="Zhengzhou University"
     gene            1026..1184
                     /locus_tag="K6159_RS30585"
     CDS             1026..1184
                     /locus_tag="K6159_RS30585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071596352.1"
                     /translation="MHEPPVQPDCCALSGNYHLESNPDGHAKTPLAAAIGNWIYTREIL
                     RGLEVGA"
                     /name="replication initiation protein"
     gene            complement(1367..1516)
                     /locus_tag="K6159_RS29970"
     CDS             complement(1367..1516)
                     /locus_tag="K6159_RS29970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_032440454.1"
                     /translation="MKKVKTIFLFILIVAGFLLAACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     gene            complement(1601..1858)
                     /locus_tag="K6159_RS29975"
     CDS             complement(1601..1858)
                     /locus_tag="K6159_RS29975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /GO_function="GO:0015643 - toxic substance binding
                     [Evidence IEA]"
                     /GO_process="GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E3-like toxin immunity protein"
                     /protein_id="WP_032440455.1"
                     /translation="MGLKLRLTWFDKKTEEFKGEEYSKDFGDDGSVIESLGMPLKDNIN
                     NGEFDLKKEWVPLLQPFFKNQIKPDVDNCYIAFDYRDGNW"
                     /name="colicin E3-like toxin immunity protein"
     gene            complement(1868..3553)
                     /locus_tag="K6159_RS29980"
     CDS             complement(1868..3553)
                     /locus_tag="K6159_RS29980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase domain-containing
                     protein"
                     /protein_id="WP_032440457.1"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSVSGDALSAAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPKMMSK
                     IMTSLPADAVTETPASTLPLDQATVRVRQRVVDVVKDERQHIAVVAGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPSLQVSVPKGVPTAKAPPKGIVAEKGDSRPAGFTAGGNSREAVIR
                     FPKETGQKPVYVSVTDVLTPAQVKQRQEEEKRRQQAWDAAHPEEGLKRDYDKAKAELDA
                     EDKNIATLNSRIASTEKALPGARAAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNEKLKAAQASLNAMNESLSRDKAALTGAMESRKQKEKKAKDAENKLNE
                     EKNKPRKGTKDYGHDYHPVPKTEDIKGLGELKRGDPKTPKQGGGGKRARWYGDKKRKIY
                     EWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPKRNIKKYL"
                     /name="colicin-like bacteriocin tRNase domain-containing
                     protein"
     gene            3880..4125
                     /locus_tag="K6159_RS29985"
     CDS             3880..4125
                     /locus_tag="K6159_RS29985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032440458.1"
                     /translation="MTHLKLTPAEEKRLADAFVAQALAMGLKGNKTLPKAVKQQVRERV
                     LRAIQDEREARRAQEMRREKYEATETTFTWQPQRRR"
                     /name="hypothetical protein"
     gene            4399..4770
                     /gene="tolA"
                     /locus_tag="K6159_RS29990"
     CDS             4399..4770
                     /gene="tolA"
                     /locus_tag="K6159_RS29990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237044.1"
                     /GO_component="GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA]"
                     /GO_function="GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="WP_001237044.1"
                     /translation="MRLIPFILLALCSPLLLTACKHSTTQPPKIYTLDKSSKISSYAAA
                     IKTAVEMQMFEPEAYAGKMCTVRISIQRDGTLNSAIAEGGDPKLCKAAISAVTHAKIPP
                     APDENTWQIFKNAPLDFRP"
                     /name="tolA"
     gene            4767..5132
                     /locus_tag="K6159_RS30340"
     CDS             4767..5132
                     /locus_tag="K6159_RS30340"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /GO_function="GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="WP_072354022.1"
                     /translation="MKAFTLTVLAAMFLTPVHAIPLKPVTTLQPTYPVQAAAKRIEGQV
                     TATFDILDDGRVDNVSLTGCHLFFRETLTAMRHWLYPAGHPAMRQVINFEFSLKNDGLQ
                     DPRPVTTQKLNDDGCRQ"
                     /name="TonB family protein"
     gene            5307..5585
                     /locus_tag="K6159_RS30000"
     CDS             5307..5585
                     /locus_tag="K6159_RS30000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014542884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family ribbon-helix-helix protein"
                     /protein_id="WP_000093040.1"
                     /translation="MSSATSIRLDEELKDRLKTLADDRHRSAHALMLEAITEYIDREEK
                     RSQYLRDGQAAWQHYQETGLHLTAEEAEAWISTWGTENEQDAPPCHR"
                     /name="CopG family ribbon-helix-helix protein"
     gene            5573..5887
                     /locus_tag="K6159_RS30005"
     CDS             5573..5887
                     /locus_tag="K6159_RS30005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001136729.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]; GO:0009432 - SOS response [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_071177730.1"
                     /translation="MPQVTISALAQRDLQRLQDFLKTKNRLAARKGGEVIVRAIQQLKT
                     LPDIGRPVPFLPLEYKELVIGFGDSGYVMLYRHDREMDQIVIVTVRHQKESGYPGADSL
                     "
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            6051..6479
                     /locus_tag="K6159_RS30010"
     CDS             6051..6479
                     /locus_tag="K6159_RS30010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001140599.1"
                     /translation="MAKTLLNLSQAAQAAGITRRTLYNHVKQGKVTVSRDGKNNPVVDV
                     SELIRVYGNVNIPEKQIPGISHRENTQKNFPQEQLLAMQKELADLRQAVTLMLEDKTSR
                     EEERRQHDDERRKLQAEVDRLTTELTQKKKRFWSGWFS"
                     /name="hypothetical protein"
     gene            6505..6684
                     /locus_tag="K6159_RS30015"
     CDS             6505..6684
                     /locus_tag="K6159_RS30015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165970.1"
                     /translation="MTKQEKTALNMAKFIKDQSLLLLEKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(6711..7241)
                     /locus_tag="K6159_RS30020"
     CDS             complement(6711..7241)
                     /locus_tag="K6159_RS30020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_071177729.1"
                     /translation="MALYMVFIEDVEDKDGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGENPQWERDKPTGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQINAMIEVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(7248..7979)
                     /gene="mobC"
                     /locus_tag="K6159_RS30025"
     CDS             complement(7248..7979)
                     /gene="mobC"
                     /locus_tag="K6159_RS30025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WP_071177728.1"
                     /translation="MTLEQYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRAKYPVDGRQVSVWGLTPHGVAFSFDEDEPLTDVIPFQP
                     SRVSAAQLPHRLAVQSLRLSMEGRGATGWRYLHRMALKGMKVPDALAELDGRTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRRANGIDEIWYICPDRPTLVRVQRAILAVDEIVNPQTGEA
                     RKTVELDRERLFACFKFMTTE"
                     /name="mobC"
     gene            complement(7979..9943)
                     /locus_tag="K6159_RS30030"
     CDS             complement(7979..9943)
                     /locus_tag="K6159_RS30030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_071177727.1"
                     /translation="MFNTDSLAWQWHIPELARQIYPWVSWLYREYYSLAGFFLVIGLFF
                     GLAELWAGRMRFVGLSHKARREYADRYKRDNNRTVPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLV
                     AGVLLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSTEQARKQQAVFLGKDEQGQPVLVPRDTWRKTNIQILGLPGSGKSAMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLV
                     AGFNLSETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     KLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFRRWPHASIMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPADFV
                     KTTVLDNTPIRWFYRAASQESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARLAFSSPLRVERREISLKTFPVLAKTDPLAEY
                     LPPADGGGPKADDDFAGLY"
                     /name="TraM recognition domain-containing protein"
     BGC             1..8553
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          697..1264
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="88.795"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(7252..7979)
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.416"
                     /coverage="100.0"
                     /evalue="1.34e-140"
ORIGIN
        1 tgattgcgtt cagctttcac cgcggaggcc agcctctttc cgccccatgc ggaaaaggcg
       61 cagccccgga ggaacaacga gcacgcccca gcgtgtgaga ggaaaaggcg gcaccccagt
      121 gcggtctttt ccggtgttcc ggcccccgtc agagttcgcc atagcgagcg gctgaccacc
      181 cgcaaaatca ggagcagctt tgctgcttcg tcaggtgcgt ccagcaccgt ggatttttgt
      241 gggtggtcag gcgtgagcct gacgggggcc gggtgaattt ctggcgtggc cggcgtccag
      301 ccggacagag ccagaaagag gggaaaagac cgtgcgtcgg tggcgtccag ccagcgtaag
      361 caaggtcttt tcccctgaag tcagccacgc cccagcgggg atgacttgca tttctccgca
      421 aggagaaaaa agcaacagag ataggttaac gtaaaatgtt cagcgaaaaa ccgacatggt
      481 tcacctatcc tgataattga tcgtcaggga atagaaagac ttaatcaggg gacaatatcc
      541 cacatcagcg cgtccggtga ccgtcttgca ggtgccgggg aaattgcgcc tcctcgctca
      601 ctcggtcgct acgctccggg cgtgagactg cggcgggcgc tgcggacaca tacaaagtta
      661 cccacagatt ccgtggataa gcaggggact aacatgtgag gcaaaacggc agggccgcgc
      721 cggtggcgtt tttccatagg ctccgccctc ctgccagagt tcacataaac agacgctttt
      781 tcggtgcatc tgtgggagcc gtgaggctca accatgaatc tgacagtacg ggcgaaaccc
      841 gacaggactt aaagatcccc accgtttccg gtaggtcgct ccctcttgcg ctctcctgtt
      901 ccgaccctgc cgtttaccgg atacctgttc cgcctttttc cctttcggga agcgtggcgc
      961 tttctcatag ctcacgctgc tggtatctcg gctcggttta ggtcgttcgc tccaagctgg
     1021 gctgtatgca cgaacccccc gttcagcccg actgctgcgc cctttccggt aactatcatc
     1081 ttgagtccaa cccggacgga cacgcaaaaa cgccactggc agcagccatt ggtaactgga
     1141 tatatacgag agagatattg agaggtcttg aagtgggtgc ctaactgcgg ctacactgaa
     1201 agaacagttt tggtatctgc ggtctcgtat acccagttac ccggttaagc agttccccaa
     1261 ctgacttaac cttcgatcaa accgcctccc caggcggttt tttcgtttac agtgcaagag
     1321 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac tgaaccgcga
     1381 tccccgtcag ttcagaggag gacgatggtg ccaccgttcc gccctgaaca tcccggatat
     1441 aatttgcctg acatgcggcc agaagaaagc cagcaacaat caatataaaa agaaaaatag
     1501 tctttacctt tttcatatac tttccctgta tttaaagact ccggcattca tccctgaaca
     1561 ccggaagtca gtatttcaaa tcatccttga taattttttg ttaccagtta ccgtcccgat
     1621 aatcaaaagc tatataacaa ttatccacat caggttttat ttgattttta aaaaatggct
     1681 gtagtaaagg aacccattct ttcttcaaat caaactcacc attattaata ttatccttta
     1741 aaggcattcc caagctttca atgacagaac catcgtcacc aaagtcttta gaatactctt
     1801 cccctttaaa ctcttcagtt ttcttatcaa accatgtcaa acgtaattta agccccatat
     1861 ttacctctca aagatatttt ttaatgttcc gttttggatc tggcccttta acctgcttac
     1921 ccgttttggg atcaaatgcg cctaggtgtt cgccatcact ggcacgatac ccttcaagct
     1981 caccgtgctg ggaatcccat tcataaattt tacgtttttt atcgccatac catcgggccc
     2041 gcttaccacc accaccttgt ttaggtgttt taggatcacc tcgctttaac tctccaagtc
     2101 cctttatgtc ttctgtttta ggtactggat gataatcatg gccgtagtct ttagtcccct
     2161 tgcgaggctt gttcttttcc tcatttaact tattttctgc atccttcgct tttttctcct
     2221 tttgtttccg gctctccatc gccccggtaa gtgcagcctt atctctggat aaggattcat
     2281 tcattgcgtt taaagatgcc tgagccgctt ttaatttctc attctgattc tgaatatcct
     2341 tatcaagata gcgaacctga tcctgccacc cggagccata ttcatgaggc gggttataag
     2401 tcacaaaatc atctttattt gcctctgcct cttttacctt tttatcggct tcctgaacag
     2461 cagcccttgc accggggagc gccttctctg tcgatgcaat gcggctgttt aaggtcgcaa
     2521 tatttttatc ttcggcatcc agctcagctt tcgctttatc atagtctctt ttcagcccct
     2581 cttccggatg agcggcgtcc catgcctgct ggcgacgctt ttcttcctcc tgacgctgtt
     2641 ttacctgtgc cggggtaaga acatctgtca ccgacacata aaccggcttc tgtccggtct
     2701 ctttcgggaa acgaataacg gcctcacggg agttaccacc ggctgtaaaa ccagccggac
     2761 gtgaatcacc tttttcagca acaatgcctt ttggcggggc tttcgctgtc ggaacacctt
     2821 tcggtacgct cacctgcaga gacgggagac ccggaatcga cacactgaat acccccggac
     2881 gttttgtcgg tttcgcatcc accacaggga cactcattgg ccgacctgcg acaaccgcaa
     2941 tatgctgccg ctcatccttc accacatcca caacccgttg tctgacacga accgtcgcct
     3001 ggtccagtgg taaagtactt gccggagtct ccgtcaccgc atcggccggt aatgacgtca
     3061 taatttttga catcattttc ggatcatctt ttgcaatctc agaaggcagc acaccgtaga
     3121 tcgcaatccc ccacagacca aacttaaacg gccccttcag ggcagccagc acatcagcaa
     3181 cggcggccga caacgcatcg ccggaaactg ataaagccag tccttcagcc cccggagtag
     3241 ccagggcagg cagaccgaag gccatcgcag tggccgaaga ctgtccgcca ccagagctac
     3301 cgctatgatt gccgctgtta ccaccattac caccgggttt actcccgccg ttaccaaact
     3361 cccccggatt atagttatga atatctccat tcggcgtatg cgtcgtaccc cagcccggcc
     3421 cgctgtttgg atccgtacca ccacctgatg aagggccacc tttaccagac gtaccgttca
     3481 cattcccact ggcctgacca ccattatgtc ccagacctga attacccgga cctcgaccgt
     3541 ctccaccact cataccgttt tcctctttga catttaaaac aataaattaa agccaacata
     3601 ctgtacatat acacagtata attattacct tactcctccc gttttcaggt gtcaatatat
     3661 gagattagtt ctcatcttca ggcacaaaaa agcgcccgga gccacctgta agcagatcgc
     3721 gtcttttccc gaaaatttct catggcaaca aattttatta tttaagacat tcatcacact
     3781 tacatttcag atggtataga cacatctgaa agctaactgg catgaggaga ggacctgcgg
     3841 ttaaactgtt cgggaaacct gagacgcccg gaggccacta tgacgcacct gaaactgact
     3901 cccgctgaag aaaagcgcct ggcggacgcc tttgtcgctc aggcactggc catgggcctg
     3961 aaagggaaca aaacgctgcc aaaggcggta aaacaacagg tgcgggaaag ggtgcttcgt
     4021 gccattcagg acgaacgtga agccaggcgg gcgcaggaaa tgcgtcggga gaaatacgag
     4081 gctacggaaa ccaccttcac ctggcagcca cagcgccgcc ggtaaggggt atttaccata
     4141 acgtcgcatt gcccgcaccg ctcagacggt ctgagggcgt ttccggcggc tacgggataa
     4201 aacaggcaac aaaccacccg gaaaaccgcc ggaaacgcgc ccggtaaatt gtaacccgct
     4261 gcatgcctat gcacgcaact gcatggcggg ccgtttggtg taaaaacgat acgataagcc
     4321 gtgtttatgt aaatgaacgt ggccgtaaag agtgacaaat atacatgtaa cattttttac
     4381 tgacaggtca tcaacccgat gcgtttaata ccctttatcc tgctggcatt gtgcagcccc
     4441 ttactcctga cggcctgtaa gcattccacc acccagccgc cgaaaattta tactctggat
     4501 aagtcatcta aaatcagtag ctacgctgcg gccatcaaga ccgctgttga aatgcagatg
     4561 tttgaaccag aggcctatgc gggaaaaatg tgcaccgtgc ggatatccat acaacgtgat
     4621 ggcactctga acagcgccat tgctgaagga ggagatccga aactctgtaa agcagcaata
     4681 tcagccgtta cccacgccaa aattccgccg gcaccggatg aaaatacctg gcaaatattc
     4741 aaaaacgccc ctctggattt cagaccatga aagcttttac tctgacagtg ctggcggcca
     4801 tgttcctcac tccggtacat gcgataccac taaaacccgt aaccacactg cagccaacct
     4861 acccggtaca ggccgcagca aaacgcatcg aaggtcaggt gaccgccacg tttgatattc
     4921 tggacgatgg acgagtggac aacgtgtcgc ttaccggctg tcaccttttt tttcgggaaa
     4981 cgctgacggc aatgaggcac tggctatatc ccgcagggca cccggcaatg cgacaggtta
     5041 taaattttga attcagtctg aaaaatgacg gcttacagga tccgcgacca gtcaccaccc
     5101 aaaagctcaa tgatgatggt tgccgccagt aacaggtcct ttaacccgca ggctgcaata
     5161 gtcctggatg atgagtgagc ctgtagcagc cctgattagt ggaagcgaag ctgaaaaatg
     5221 ggcagcaaaa gccgtatttc tggcaaatag cgccgccacg gattaaagta gtcatgtgta
     5281 atacatatga ctacaaaggg gcttttatgt catcagcaac atccatcagg ctcgatgaag
     5341 aacttaagga ccggctgaaa acgctggccg atgaccggca ccgctcagcg cacgcgctaa
     5401 tgctggaggc aatcaccgaa tacatcgacc gggaagagaa gcgcagccag tacctgcggg
     5461 acggacaggc cgcatggcag cactatcagg aaacgggtct gcaccttact gcggaagaag
     5521 cggaagcctg gataagtacc tggggaactg agaacgagca ggatgctccg ccatgccaca
     5581 ggtaacgatt tccgcgctgg cacagcgcga tttacaacgc cttcaggact ttctcaaaac
     5641 caaaaatcgg ctggcagcca gaaaaggagg tgaggtgatc gtccgggcta tccagcaact
     5701 gaaaacattg ccagacattg gccgcccggt gccgtttctg ccgctggaat ataaggaact
     5761 ggtgatcggg tttggcgaca gtggctatgt gatgctctac cgccacgacc gggaaatgga
     5821 ccagattgtg atcgtcaccg tcaggcatca gaaagaatcc gggtatccgg gggcagacag
     5881 cctctaataa ccgatgacgg gggcgcaatt cattgcgtgg ccgtcgccgg tttgacaagc
     5941 gcagcgcgtc agaaaaccac atcctgaacg gaaaaacggg taaaattcag cgatacattt
     6001 accgtgagaa attacacacc tttcacaccc gataaaatca ggggtataca gtggcgaaaa
     6061 cattgctcaa cctttcacag gcagcacaag ccgcgggcat taccagaaga acgctataca
     6121 atcatgtaaa acagggaaag gttaccgtgt cacgtgacgg gaaaaacaac cctgtcgtgg
     6181 atgtgtcaga actgatccgt gtatacggaa atgtgaatat ccctgagaaa caaattcccg
     6241 gcatttcaca ccgtgaaaat acacaaaaaa atttcccaca ggaacagctt ctggcaatgc
     6301 aaaaggaact ggccgatctc cggcaggctg tcaccctgat gctggaagat aaaacatccc
     6361 gggaagaaga acgccgccag cacgatgatg agcgccggaa actccaggcc gaagtggaca
     6421 gattgacaac agaactgaca cagaagaaaa aacgcttctg gtccggctgg tttagctgag
     6481 aacttaccga agggaatgga gaacatgacg aagcaggaaa aaactgccct caatatggct
     6541 aaattcatta aggatcagtc gctgctgttg ctggaaaagc tgaacgagct ggacatggat
     6601 accccggccg acatgtgcga acggcttcac gaggatgcgg aaacactctg gcaggtgatg
     6661 cgggataagc tcggggaaga ataacagcac cggccatgcc ggtgctggcg ttattttttg
     6721 ccgaacggcc accaccggcg ggcgggtttg tcctccggca gttcaggcac cggcacgtta
     6781 aaatagcggc tgttatattc ccgcatcagc tccgctttca gcttctcgtt caccgcgctt
     6841 tccccgtgcc gctcgctgac cacctcaatc atcgcgttaa tctgctccgg tgagcacttc
     6901 ccggcatcaa ccacctgcac accgttctcg atggcgaacc gctccacctt tcgatagagg
     6961 taatcacaca gcttcattcc tgtgggtttg tcccgttccc attgcggatt ttcaccggtg
     7021 aaaaagtacg tggctttttc gtcgctgttg agccgctcgt catacatccc gatggatttc
     7081 atcatcgcca gatgctgggc ccggtcgaca aaacagaaaa catacgcccc ggtctgccag
     7141 gcgagcacca ccatgtcttc cagatgctcg ctggttggca taatgcagcg ctcgccgtct
     7201 ttgtcttcca cgtcctcaat aaataccatg tacagcgcca taatccctca ctccgttgtc
     7261 ataaatttaa agcacgcgaa cagcctctcc cggtccagct ccaccgtttt gcgggcctcg
     7321 ccggtctgtg gattcacgat ttcatccacc gccagaatcg cccgctgtac ccggacaagc
     7381 gtcggccggt cagggcagat gtaccagatt tcatcgatgc cgttcgcccg gcggttgaac
     7441 agatacccgg acaccacttc ctgataccgc ctgcggctct tgacggtgcg ctctacctca
     7501 aatgccaccg ttctgccgtc cagctccgcc agcgcgtccg gcactttcat ccctttcagg
     7561 gccatacggt gcaggtaccg ccagccggtg gccccccggc cctccatgga gaggcgcagg
     7621 gactgcacgg ccagccggtg cggcagctgc gccgccgaca cccttgacgg ctgaaacgga
     7681 ataacgtccg tcagcggctc gtcctcgtcg aacgagaacg ccaccccgtg cggcgtcagc
     7741 ccccacacgc tgacctgacg gccgtcaacc gggtatttcg cccggaccag cagccctttc
     7801 gccaccagac tgtccaggaa gcggtaaacc ggctgtaccg ccgcaatccc cagccgtcgc
     7861 gcaaccagct ccgccgtgga acacagctct tcccgcagcc agcgcagggt cagccgtgtt
     7921 ttctccgcgt tccgcgcctg ccgcttcgcg tggctgacgt tgtactgctc cagtgtcatc
     7981 agtaaagccc cgcaaaatca tcatccgctt ttggtccgcc gccgtcggcc ggaggcaggt
     8041 actccgccag cgggtccgtt ttcgccagca ccgggaaggt tttcagcgaa atttcccggc
     8101 gctcaacccg cagcgggctg ctgaacgcca gccgcgccac gcccagcccg gtcatcaccg
     8161 cgaacccgtc aggcaggtgt tgcagggtgt tcacgtcaaa cagcggccgc gcctccttct
     8221 ggatgaagct gtcgccgctg atgtgctcca cgttccccgc ctcccggctg gcccggcggc
     8281 gctccacgtc cacccggatc tccccggtct ggcccgccgc ccactgcgcg gactcctggc
     8341 tggccgcgcg atagaaccag cggatcggcg tgttatccag taccgtggtt ttcacaaaat
     8401 cagcgggcag atcctgcccg cactgaccga agtcgcccag cgactggtgc gcgagcagca
     8461 ggttgcagtc ccggctgcgc agcgtcccca gcgcgttcag cacgtacttc gacagcagat
     8521 atttaatctc atcgagcatg atgctcgcgt gcggccacct ccggaactca tcccgcgcaa
     8581 tgatgatttg cgcgcagcgg gcaaacagca tcttctgcac ccggatcacc gcctcatcgt
     8641 ccatcgagcc gatgacgtac aggcagccgc cgccgttgat tatccccgcc acatcgatac
     8701 cgctgtccgt ctgcaggacg ctcaggtcgg ccacgttctc cagcttggtg atcagcccct
     8761 tcacgtcctt tttcagggcc tccggcaacg cgtacgccgc cgccagcacc tgctgaatgt
     8821 ttgccccctg cgggaactgc tccgcaatca gcttcgccgc tttctgctcg ctgatgcggt
     8881 agtggtccgc cacgtcgccg gtctcactca ggttaaaccc ggcaaccagc agattcttca
     8941 gggcgtactg gtcgagatcg cggaacagat tcagctgcgc cggtttcccc ggccgcagat
     9001 ccagcagggt gaagtccggg cagtgcgccc ggaagacgtg cggcgcccac gcgtcccctt
     9061 tggggtcgaa gtacaccaca gcgtccccga aatcgcgtac gcagcgaatg agcgcgttgg
     9121 tggccatcgc gctcttgccg ctcccgggca ggccgaggat ctgaatattg gtcttgcgcc
     9181 aggtatcacg agggaccagc accggctggc cctgctcatc cttacccaga aacaccgcct
     9241 gctgtttccg ggcctgctcc gtggaaaaat cggccagcgc gttaaccggt accggctccc
     9301 cgaaatgcag gtggcggacg tctgtcattt ctccggtccg ctggctgctg cgccggctct
     9361 cctgattcag tttcgcgttc accgcctcgt cagcggccgc aaaatgacca tcaagcagca
     9421 ggaacgccag cagccgcgcc agaaggacgc cggcgacaag ccctccggca caccaggcta
     9481 tcccgaacag gttagccggc tgcagcaggt aataatctgc gttcgcatag aggtccgggc
     9541 gcaggacggt ttctccccag ctctcgataa acacggcgac aaccagcccc cccagaaggg
     9601 ccagaccggc agcgccggtc agccagtacg cgtagttcca gacatgtcgc cgtgccctga
     9661 ccatccgccc gggtgaatcc agcgaggcca gcaccgaaaa tggcacagtc cggttattat
     9721 cgcgcttata gcgatcggca tactcccgcc gcgccttatg gctcagcccc acaaaacgca
     9781 ttcggccggc ccagagttca gccagaccga aaaacaggcc gataaccaga aaaaaaccgg
     9841 ccagactgta atactcccgg tacagccagc tgacccacgg gtaaatctgc cgggccagct
     9901 ccgggatatg ccactgccag gcaagcgaat ccgtattaaa catgcaatct ctctccttca
     9961 gacgacgggt ttaccggcgt gatgcgcagc ccccgctgca caagccgcgg acatgcccca
    10021 aggcatggcg gcggcggtca cgtccgtgg
//
