LOCUS       NZ_CP082018             5596 bp    DNA     circular CON 10-JAN-2024
DEFINITION  Klebsiella pneumoniae strain KP18-2050 plasmid pKP18-2050-4,
            complete sequence.
ACCESSION   NZ_CP082018
VERSION     NZ_CP082018.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN20928633
            Assembly: GCF_019823835.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 5596)
  AUTHORS   Qin,S. and Dong,H.
  TITLE     Klebsiella pneumoniae strains
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5596)
  AUTHORS   Qin,S. and Dong,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2021) Institute of Drug Discovery & Development,
            Zhengzhou University, 100 Science Avenue, Zhengzhou City, Zhengzhou,
            Henan 450001, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: unicycler v. v.0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 12x
            Sequencing Technology             :: Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/10/2024 03:38:20
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,056
            CDSs (total)                      :: 5,934
            Genes (coding)                    :: 5,704
            CDSs (with protein)               :: 5,704
            Genes (RNA)                       :: 122
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 82 of 230
            Pseudo Genes (incomplete)         :: 163 of 230
            Pseudo Genes (internal stop)      :: 29 of 230
            Pseudo Genes (multiple problems)  :: 40 of 230
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP082018.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5596
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="KP18-2050"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pKP18-2050-4"
                     /geo_loc_name="China"
                     /lat_lon="34.73 N 113.70 E"
                     /collection_date="2018-11-13"
                     /collected_by="Zhengzhou University"
     gene            9..518
                     /locus_tag="K6159_RS00005"
     CDS             9..518
                     /locus_tag="K6159_RS00005"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_032433972.1"
                     /translation="MNSLLTLAKDLEQKSKAQQQSTGEMLKAAFSEHEKSVRAELSESG
                     KRISAAIRAHEQGMSAAMQSNRLSVMRMVGRTWLTITMVSGLLFASLSGVLWYQGSLIA
                     SNLAEIDRQNAALSKLNAKTWGVTYLEDSNGRFLVLPKGTAVDRTQSWTVGNGRSKQNA
                     LRLVKE"
                     /name="MbeB family mobilization protein"
     gene            525..737
                     /locus_tag="K6159_RS00010"
     CDS             525..737
                     /locus_tag="K6159_RS00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_021527181.1"
                     /translation="MTELEKQLLSALEQLQQDYSKRLDEWESAFGEWRKMSGLMQRENA
                     ALSERVTRLSQQVERLSGQLQRLSR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(1113..1241)
                     /locus_tag="K6159_RS30345"
     CDS             complement(1113..1241)
                     /locus_tag="K6159_RS30345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000523570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000523570.1"
                     /translation="MGLKPGPKRIADSTGEPDKRQRDNKKTPGNTDKLKPSKSSKK"
                     /name="hypothetical protein"
     gene            1404..1628
                     /locus_tag="K6159_RS00015"
     CDS             1404..1628
                     /locus_tag="K6159_RS00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6290 family protein"
                     /protein_id="WP_000879771.1"
                     /translation="MLAIRLSDEIESRLDSLAKQTGRTKTFYAREAILAHLEDLEDYYL
                     SAETAARVRRGDEAVHSSEDVRKSLGLDD"
                     /name="DUF6290 family protein"
     gene            1612..1878
                     /locus_tag="K6159_RS00020"
     CDS             1612..1878
                     /locus_tag="K6159_RS00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262326.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_004197762.1"
                     /translation="MVWTINYSDRALKSLRKMDKQNARRIVDFMSLRIAVAADPRQSGK
                     PLKGELGEFWRYRVGDYRVLCEIRDDELVILAATIGHRREVYD"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(1948..2298)
                     /locus_tag="K6159_RS00025"
     CDS             complement(1948..2298)
                     /locus_tag="K6159_RS00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004197756.1"
                     /translation="MYWLIAFLAFIFPGRSFADGYTAYYRCNGTQEASLTVKDNKLSMV
                     VGLSKFNRQLDTYRFKEKGIEVVMQRAASDDNSEAIMSFIMPDVNNMTLLVKNNGYIIV
                     KTTCWLTKTDKQ"
                     /name="hypothetical protein"
     gene            complement(2364..2909)
                     /locus_tag="K6159_RS00030"
     CDS             complement(2364..2909)
                     /locus_tag="K6159_RS00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_023317533.1"
                     /translation="MFFKRYLTEKEIYKAALPIVRLQGRVNTSLRFLSKYSDVNKNGLI
                     ALHLNLLHFISYNIHGVESLKITQAGINKAVQQSPHADEIKNIAQAIGRKGPEYIEGLI
                     RNPWGKKNDNPLIFIMSIEESIKETLNSGYMLYRVAPITEGEAAAFMDVLKQLLLFEDN
                     FRLFGRDLVYKIEKLKIR"
                     /name="hypothetical protein"
     gene            complement(2935..3261)
                     /locus_tag="K6159_RS00035"
     CDS             complement(2935..3261)
                     /locus_tag="K6159_RS00035"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_050516389.1"
                     /translation="MRERTNIMLDIGLKQSLQRLSNKTKMSLSDLINQRLSGSLSVEER
                     IGEKPEWMRNLDPYLANHFSNIELPLPSPLKEYQEKMDEIDSIINDMMVLKEYFRAKFY
                     DEIK"
                     /name="hypothetical protein"
     gene            complement(3619..3777)
                     /locus_tag="K6159_RS30430"
     CDS             complement(3619..3777)
                     /locus_tag="K6159_RS30430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071596352.1"
                     /translation="MHEPPVQPDCCALSGNYHLESNPDGHAKTPLAAAIGNWIYTREIL
                     RGLEVGA"
                     /name="replication initiation protein"
     gene            4417..4593
                     /locus_tag="K6159_RS00040"
     CDS             4417..4593
                     /locus_tag="K6159_RS00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004197757.1"
                     /translation="MQKPKKKPLVVKKRSWHPAVMANLMRQAQEEDYRREQMAELERSA
                     RSHFADIGGEDGG"
                     /name="hypothetical protein"
     gene            4583..4927
                     /locus_tag="K6159_RS00045"
     CDS             4583..4927
                     /locus_tag="K6159_RS00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006122514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_062955149.1"
                     /translation="MAVKRTRFLGIRVTDGEYQQLLERCNGRQLAVWMRETCLDTRPAR
                     SLRLPSIDPVLLRQLAGMGNNLNQIARKINGGQWSGADRVQVVAALMAIDAELERLRHA
                     VLEKGADDDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            complement(5109..5354)
                     /locus_tag="K6159_RS30035"
     CDS             complement(5109..5354)
                     /locus_tag="K6159_RS30035"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032433971.1"
                     /translation="MQPQSPVHHGLPRIDARTVCPVVVRLETPAGQQFRVRYQKIQFQP
                     PLVPVFNPQHAVLVFIESGHQNPLEARHQLFTLAGR"
                     /name="hypothetical protein"
     Typing          4252..4331
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="90.361"
                     /coverage="97.0"
                     /evalue="1.1100000000000001e-21"
     Typing          complement(3669..4066)
                     /name="rep_cluster_2370"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="89.526"
                     /coverage="91.0"
                     /evalue="3.03e-139"
     Typing          3..853
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.648"
                     /coverage="99.0"
                     /evalue="1.23e-161"
     Typing          4918..5594
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="99.0"
                     /evalue="3.3000000000000005e-145"
ORIGIN
        1 agccatctat gaacagtctt ttaacgctgg cgaaggactt agagcagaaa tcgaaagcgc
       61 agcagcagag taccggcgaa atgctgaaag ccgcattcag cgagcacgag aagtctgtca
      121 gagcggaact gagcgaaagc gggaagagaa tcagcgccgc catccgcgcc cacgagcagg
      181 gtatgagcgc ggccatgcag tcgaaccgcc tgagcgtgat gcgtatggtc gggcggacat
      241 ggctgaccat cacgatggtg tcaggactgc tgttcgccag cctgagcggg gtgttgtggt
      301 atcaggggag cttgatagcg tcaaatctgg ccgaaatcga ccggcagaac gcagcgctgt
      361 cgaagctgaa cgcgaagacc tggggcgtga cgtacctgga agacagcaac ggacgttttc
      421 tggtgctgcc gaaggggacg gccgtagaca ggacgcagag ctggacggtc gggaacgggc
      481 ggtcgaagca gaacgcgctg agactggtga aggagtaagt gccgatgacc gagctggaaa
      541 aacagttgct gagcgcatta gagcagctac agcaggacta ctcgaaaagg ctggacgagt
      601 gggagagcgc cttcggggaa tggcggaaaa tgtcagggct tatgcaacgg gagaacgcgg
      661 cgctgagcga gcgcgtgacg cgcttgagtc agcaggtgga acgcttgagc gggcaactgc
      721 agcgtttgag ccggtagtgc agcgtcatga gatggccgtg gccgcagaac gggcgcacga
      781 gcaacggcag cacgaaaagg aactgacgga agcgcgttcc cggcagcgaa gctatgacgg
      841 tccgtcgctg ggctgaaatg caggaaaaca gggttctcat cgggcattta cgctgaaacc
      901 ggatgtcatt cacctggctg catggctatg cagccaggta aaatttatct gtcgcgaacg
      961 gggcggttta tcgggtggtt tgttgccggt tttacctgac tgccaccccg ttcgcggcga
     1021 acccgtccgg ggcggtgcgg gcaacgggtg ttatgtttaa tcagcgtgaa cggcagatac
     1081 aaaaaagccc cggagggctt ttaactactg acttattttt ttgaagattt gctgggtttc
     1141 aatttgtcag tatttcccgg cgttttttta ttgtcacgct ggcgtttatc aggttcccct
     1201 gttgaatcgg caattcgttt cggacccggc ttaagaccca taatcgttat cctttcaata
     1261 gttaagagga aagccctcaa gctagatata ttgaccgcgt tacggattat caagaggggc
     1321 taacaagatt attttttgtt cttttaggtt gattgtggta ctgttgatat acatgtatag
     1381 caaatggtgt tcgggaggtt tatatgcttg ctatccggtt atctgatgag attgagtccc
     1441 gtctggactc gctggcgaag caaaccggca gaacaaagac gttttatgcg cgggaagcaa
     1501 tactggcgca tctggaagac ctggaggatt attatctttc agcagaaact gctgcacgcg
     1561 ttcgccgtgg tgatgaagca gtgcattcgt ctgaagacgt gaggaagtca cttggtctgg
     1621 acgattaact attccgatcg ggcgctcaaa tcgttacgca agatggacaa acagaacgca
     1681 cgacggattg tggattttat gagtttacgc attgcagttg ctgccgatcc tcgccagtca
     1741 gggaagccgc tcaaaggtga gctgggcgag ttctggcgct atcgggtggg agattatcgc
     1801 gttctgtgtg agatccgaga tgacgagctt gttatccttg ccgccacgat tggacatcgc
     1861 cgcgaagttt acgactgaag cccgctatgc gggctttttt gatgccgaca acaggtatta
     1921 tggtaaatac tcgactaact acgtgttcta ctgtttgtcg gttttagtta accagcaggt
     1981 tgtcttaacg ataatgtagc cgttattttt aactaataag gtcatattgt taacgtcagg
     2041 cataataaaa gacattatgg cctcagagtt atcatcagaa gcagctctct gcattactac
     2101 ttcgattccc ttttctttga atctataagt atccagttgc ctattaaact tagaaagacc
     2161 tacaaccata cttagtttgt tatctttaac agtaagagat gcttcctgag taccattaca
     2221 tcggtagtaa gcggtgtaac cgtcagcaaa agatcgaccg gggaatataa atgctaggaa
     2281 cgcaattaac caatacatag ttgagaatct tggtattaca catttcacta tcctctcttt
     2341 catttttcgt tcaccatact ttgttatctg atttttaatt tttctatttt ataaaccaag
     2401 tctctaccaa aaaggcgaaa attatcctca aataataata attgtttaag aacatccatg
     2461 aaagcagctg cttcaccctc agttatggga gctactctat ataacatata acctgagtta
     2521 agggtttctt ttatactttc ttcaatgctc attataaata ttagtggatt atcatttttc
     2581 ttcccccatg ggtttctaat taatccttcg atgtactcag gtcctttgcg tcctattgcc
     2641 tgcgcaatat tttttatttc atctgcgtga gggctttgtt gtacagcttt gtttatgcct
     2701 gcttgagtga ttttcagaga ctctacaccg tgtatgttgt atgagataaa atgtaataaa
     2761 ttaagatgca atgcaattaa tccatttttg tttacatctg agtatttgga taaaaaacgt
     2821 aatgaagtgt taactctccc ttgaagccta acaattggga gtgctgcctt gtatatttcc
     2881 ttctccgtta aataccgttt aaaaaacata attctccgcc tccgtatagc gtgcctattt
     2941 tatttcgtcg taaaacttag ctctgaaata ttcctttaga accatcatgt cattaataat
     3001 tgaatctatc tcatccattt tttcttggta ttcttttaat ggtgaaggga gcggtaactc
     3061 aatatttgaa aagtgattgg cgagataggg atcaaggttt ctcatccatt caggcttttc
     3121 gccaatgcgt tcttcaactg aaagtgaacc tgataatctc tggttaataa gatcacttag
     3181 actcattttg gttttattcg aaagccgttg taaggattgc tttaatccta tatctagcat
     3241 gatatttgtt ctttctctca ttaccccccc ttgctattgc gttttttgtt gcgcacaaag
     3301 ctaccattgc gcattaagaa acgcaataaa aaaggaggga gggagtggtt tgtacatgat
     3361 gtgcagtgcg ctgagggcat cagtggcggg tgtttttccg tgggatggat ggagctccgg
     3421 ggataacaga actgacgatc atttaaagga tcttcttgag atcctttttt tctgcgcgta
     3481 atctcttgca ctgtaaacga aaaaaccgcc tggggaggcg gtttgatcga aggttaagtc
     3541 agttggggaa ctgcttaacc gggtaactgg gtatacgaga ccgcagatac caaaactgtt
     3601 ctttcagtgt agccgcagtt aggcacccac ttcaagacct ctcaatatct ctctcgtata
     3661 tatccagtta ccaatggctg ctgccagtgg cgtttttgcg tgtccgtccg ggttggactc
     3721 aagatgatag ttaccggaaa gggcgcagca gtcgggctga acggggggtt cgtgcataca
     3781 gcccagcttg gagcgaacga cctaaaccga gccgagatac cagcagcgtg agctatgaga
     3841 aagcgccacg cttcccgaaa gggaaaaagg cggaacaggt atccggtaaa cggcagggtc
     3901 ggaacaggag agcgcacgag ggagccgccg gagagaaatc accggcatct ttaagtcctg
     3961 tcgggtttcg tcctcctctg acttgagcgt ccatttctgt gatgctcgtc aggggaggcg
     4021 gagcctatgg aaaaacggca acgccgcatc cttttctccg gccctttctg atttgagcgc
     4081 ctttgcttta tcccgttatc cgtgagtcgg tcacgcccgc cgcagccgaa cggcaggagc
     4141 gcagctaccg agtgagcgag gaggcgtcat ttcatctgtc cgtgcatggt attgcggacg
     4201 ctcctgagcc acatatcaca cctgccacat gaggcacttt catgaatcgt gctctcatgc
     4261 caacatagta agccagtata cacagcgtaa acgctgtgac tggttcaggg ctgcgccccg
     4321 aaacccgcta acaccactga cgcgctggcg cttgtccaac gccggagcga cccggaaaac
     4381 ttttccgtgc cgtccggcgt ttgcgaagga gggataatgc agaagccgaa gaagaagccg
     4441 ctcgtcgtaa aaaaaagatc ctggcatccg gcagtgatgg caaatctgat gcgtcaggca
     4501 caggaggaag attaccgccg ggagcagatg gcagagcttg agcgttcagc ccggtcacat
     4561 tttgcggata tcggaggaga ggatggcggt taagcgtacc cgttttttag gtatccgggt
     4621 gaccgatggg gagtaccagc agctgctgga gcgctgcaat ggaaggcaac tggcggtgtg
     4681 gatgcgggaa acctgtctcg atacgcgccc ggcgcggtcg ttgcggctgc cgtccatcga
     4741 tccggttttg ctgcgccagc ttgccgggat ggggaacaac ctcaaccaga tcgcccggaa
     4801 aattaacggc ggccagtggt caggcgctga ccgggttcag gtggtggccg cgctgatggc
     4861 cattgatgcc gaactcgagc ggctgcggca tgccgtgctg gaaaaggggg cagacgatga
     4921 tcgttaagtt tcatccgcga gggcgaggcg gcggtgccgg gccggtggat tatctgctgg
     4981 ggaaagaccg ccagcgcgac ggggccatcg tcctgcaggg aaagccggag gaggtccggg
     5041 agcttatcga cgcctcgccc tacgccaaaa agtacacctc cggcgtcctg tcttttgctg
     5101 aacaggattt accgcccggc cagcgtgaaa agctgatggc gagcttcgag cgggttctga
     5161 tgcccggact cgataaagac cagtacagcg tgctgtgggt tgaacaccgg gacaaggggc
     5221 ggctggaact gaattttctg gtaccgaaca cggaactgct gaccggcagg cgtctccagc
     5281 cgtactacga ccgggcagac cgtccgcgca tcgatgcgtg gcagaccgtg gtgaacgggc
     5341 gactggggct gcacgacccg aacgcaccgg agaaccggcg cgcgctggtg acgccatctt
     5401 cgttacccaa aacgaagcag gaagccgcag aggctattac gcggggctta ctggccctcg
     5461 cctcgtcagg ggagcttaaa acgcgtcagg acgtcactga ggcgctggaa agcgcaggtt
     5521 ttgaggtcgt gcgcaccaca aagagcagca tcagcattgc cgacccggac ggagggcgaa
     5581 acatccgact taaggg
//
