LOCUS       NZ_CP082017            11192 bp    DNA     circular CON 10-JAN-2024
DEFINITION  Klebsiella pneumoniae strain KP18-238 plasmid pKP18-238-4, complete
            sequence.
ACCESSION   NZ_CP082017
VERSION     NZ_CP082017.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN20928632
            Assembly: GCF_019823855.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 11192)
  AUTHORS   Qin,S. and Dong,H.
  TITLE     Klebsiella pneumoniae strains
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11192)
  AUTHORS   Qin,S. and Dong,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2021) Institute of Drug Discovery & Development,
            Zhengzhou University, 100 Science Avenue, Zhengzhou City, Zhengzhou,
            Henan 450001, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: unicycler v. v.0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 12x
            Sequencing Technology             :: Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/10/2024 03:26:04
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,882
            CDSs (total)                      :: 5,759
            Genes (coding)                    :: 5,600
            CDSs (with protein)               :: 5,600
            Genes (RNA)                       :: 123
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 61 of 159
            Pseudo Genes (incomplete)         :: 106 of 159
            Pseudo Genes (internal stop)      :: 14 of 159
            Pseudo Genes (multiple problems)  :: 22 of 159
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP082017.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..11192
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="KP18-238"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pKP18-238-4"
                     /geo_loc_name="China"
                     /lat_lon="34.73 N 113.70 E"
                     /collection_date="2018-10-04"
                     /collected_by="Zhengzhou University"
     gene            complement(263..421)
                     /locus_tag="K6162_RS29680"
     CDS             complement(263..421)
                     /locus_tag="K6162_RS29680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071596352.1"
                     /translation="MHEPPVQPDCCALSGNYHLESNPDGHAKTPLAAAIGNWIYTREIL
                     RGLEVGA"
                     /name="replication initiation protein"
     gene            1061..1237
                     /locus_tag="K6162_RS29075"
     CDS             1061..1237
                     /locus_tag="K6162_RS29075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004197757.1"
                     /translation="MQKPKKKPLVVKKRSWHPAVMANLMRQAQEEDYRREQMAELERSA
                     RSHFADIGGEDGG"
                     /name="hypothetical protein"
     gene            1227..1571
                     /locus_tag="K6162_RS29080"
     CDS             1227..1571
                     /locus_tag="K6162_RS29080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006122514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_062955149.1"
                     /translation="MAVKRTRFLGIRVTDGEYQQLLERCNGRQLAVWMRETCLDTRPAR
                     SLRLPSIDPVLLRQLAGMGNNLNQIARKINGGQWSGADRVQVVAALMAIDAELERLRHA
                     VLEKGADDDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            1780..>2214
                     /locus_tag="K6162_RS29535"
                     /pseudo=""
     CDS             1780..>2214
                     /locus_tag="K6162_RS29535"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            2249..2758
                     /locus_tag="K6162_RS29090"
     CDS             2249..2758
                     /locus_tag="K6162_RS29090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_032433972.1"
                     /translation="MNSLLTLAKDLEQKSKAQQQSTGEMLKAAFSEHEKSVRAELSESG
                     KRISAAIRAHEQGMSAAMQSNRLSVMRMVGRTWLTITMVSGLLFASLSGVLWYQGSLIA
                     SNLAEIDRQNAALSKLNAKTWGVTYLEDSNGRFLVLPKGTAVDRTQSWTVGNGRSKQNA
                     LRLVKE"
                     /name="MbeB family mobilization protein"
     gene            2765..2977
                     /locus_tag="K6162_RS29095"
     CDS             2765..2977
                     /locus_tag="K6162_RS29095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_021527181.1"
                     /translation="MTELEKQLLSALEQLQQDYSKRLDEWESAFGEWRKMSGLMQRENA
                     ALSERVTRLSQQVERLSGQLQRLSR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(3353..3481)
                     /locus_tag="K6162_RS29540"
     CDS             complement(3353..3481)
                     /locus_tag="K6162_RS29540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000523570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000523570.1"
                     /translation="MGLKPGPKRIADSTGEPDKRQRDNKKTPGNTDKLKPSKSSKK"
                     /name="hypothetical protein"
     gene            3644..3868
                     /locus_tag="K6162_RS29100"
     CDS             3644..3868
                     /locus_tag="K6162_RS29100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6290 family protein"
                     /protein_id="WP_000879771.1"
                     /translation="MLAIRLSDEIESRLDSLAKQTGRTKTFYAREAILAHLEDLEDYYL
                     SAETAARVRRGDEAVHSSEDVRKSLGLDD"
                     /name="DUF6290 family protein"
     gene            3852..4118
                     /locus_tag="K6162_RS29105"
     CDS             3852..4118
                     /locus_tag="K6162_RS29105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262326.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_004197762.1"
                     /translation="MVWTINYSDRALKSLRKMDKQNARRIVDFMSLRIAVAADPRQSGK
                     PLKGELGEFWRYRVGDYRVLCEIRDDELVILAATIGHRREVYD"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(4188..4538)
                     /locus_tag="K6162_RS29110"
     CDS             complement(4188..4538)
                     /locus_tag="K6162_RS29110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004197756.1"
                     /translation="MYWLIAFLAFIFPGRSFADGYTAYYRCNGTQEASLTVKDNKLSMV
                     VGLSKFNRQLDTYRFKEKGIEVVMQRAASDDNSEAIMSFIMPDVNNMTLLVKNNGYIIV
                     KTTCWLTKTDKQ"
                     /name="hypothetical protein"
     gene            complement(4604..5149)
                     /locus_tag="K6162_RS29115"
     CDS             complement(4604..5149)
                     /locus_tag="K6162_RS29115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_023317533.1"
                     /translation="MFFKRYLTEKEIYKAALPIVRLQGRVNTSLRFLSKYSDVNKNGLI
                     ALHLNLLHFISYNIHGVESLKITQAGINKAVQQSPHADEIKNIAQAIGRKGPEYIEGLI
                     RNPWGKKNDNPLIFIMSIEESIKETLNSGYMLYRVAPITEGEAAAFMDVLKQLLLFEDN
                     FRLFGRDLVYKIEKLKIR"
                     /name="hypothetical protein"
     gene            complement(5175..5501)
                     /locus_tag="K6162_RS29120"
     CDS             complement(5175..5501)
                     /locus_tag="K6162_RS29120"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_050516389.1"
                     /translation="MRERTNIMLDIGLKQSLQRLSNKTKMSLSDLINQRLSGSLSVEER
                     IGEKPEWMRNLDPYLANHFSNIELPLPSPLKEYQEKMDEIDSIINDMMVLKEYFRAKFY
                     DEIK"
                     /name="hypothetical protein"
     gene            complement(5859..6017)
                     /locus_tag="K6162_RS29685"
     CDS             complement(5859..6017)
                     /locus_tag="K6162_RS29685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071596352.1"
                     /translation="MHEPPVQPDCCALSGNYHLESNPDGHAKTPLAAAIGNWIYTREIL
                     RGLEVGA"
                     /name="replication initiation protein"
     gene            6657..6833
                     /locus_tag="K6162_RS29125"
     CDS             6657..6833
                     /locus_tag="K6162_RS29125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004197757.1"
                     /translation="MQKPKKKPLVVKKRSWHPAVMANLMRQAQEEDYRREQMAELERSA
                     RSHFADIGGEDGG"
                     /name="hypothetical protein"
     gene            6823..7167
                     /locus_tag="K6162_RS29130"
     CDS             6823..7167
                     /locus_tag="K6162_RS29130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006122514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_062955149.1"
                     /translation="MAVKRTRFLGIRVTDGEYQQLLERCNGRQLAVWMRETCLDTRPAR
                     SLRLPSIDPVLLRQLAGMGNNLNQIARKINGGQWSGADRVQVVAALMAIDAELERLRHA
                     VLEKGADDDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            7376..>7810
                     /locus_tag="K6162_RS29545"
                     /pseudo=""
     CDS             7376..>7810
                     /locus_tag="K6162_RS29545"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            7845..8354
                     /locus_tag="K6162_RS29140"
     CDS             7845..8354
                     /locus_tag="K6162_RS29140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_032433972.1"
                     /translation="MNSLLTLAKDLEQKSKAQQQSTGEMLKAAFSEHEKSVRAELSESG
                     KRISAAIRAHEQGMSAAMQSNRLSVMRMVGRTWLTITMVSGLLFASLSGVLWYQGSLIA
                     SNLAEIDRQNAALSKLNAKTWGVTYLEDSNGRFLVLPKGTAVDRTQSWTVGNGRSKQNA
                     LRLVKE"
                     /name="MbeB family mobilization protein"
     gene            8361..8573
                     /locus_tag="K6162_RS29145"
     CDS             8361..8573
                     /locus_tag="K6162_RS29145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_021527181.1"
                     /translation="MTELEKQLLSALEQLQQDYSKRLDEWESAFGEWRKMSGLMQRENA
                     ALSERVTRLSQQVERLSGQLQRLSR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(8949..9077)
                     /locus_tag="K6162_RS29550"
     CDS             complement(8949..9077)
                     /locus_tag="K6162_RS29550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000523570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000523570.1"
                     /translation="MGLKPGPKRIADSTGEPDKRQRDNKKTPGNTDKLKPSKSSKK"
                     /name="hypothetical protein"
     gene            9240..9464
                     /locus_tag="K6162_RS29150"
     CDS             9240..9464
                     /locus_tag="K6162_RS29150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6290 family protein"
                     /protein_id="WP_000879771.1"
                     /translation="MLAIRLSDEIESRLDSLAKQTGRTKTFYAREAILAHLEDLEDYYL
                     SAETAARVRRGDEAVHSSEDVRKSLGLDD"
                     /name="DUF6290 family protein"
     gene            9448..9714
                     /locus_tag="K6162_RS29155"
     CDS             9448..9714
                     /locus_tag="K6162_RS29155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262326.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_004197762.1"
                     /translation="MVWTINYSDRALKSLRKMDKQNARRIVDFMSLRIAVAADPRQSGK
                     PLKGELGEFWRYRVGDYRVLCEIRDDELVILAATIGHRREVYD"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(9784..10134)
                     /locus_tag="K6162_RS29160"
     CDS             complement(9784..10134)
                     /locus_tag="K6162_RS29160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004197756.1"
                     /translation="MYWLIAFLAFIFPGRSFADGYTAYYRCNGTQEASLTVKDNKLSMV
                     VGLSKFNRQLDTYRFKEKGIEVVMQRAASDDNSEAIMSFIMPDVNNMTLLVKNNGYIIV
                     KTTCWLTKTDKQ"
                     /name="hypothetical protein"
     gene            complement(10200..10745)
                     /locus_tag="K6162_RS29165"
     CDS             complement(10200..10745)
                     /locus_tag="K6162_RS29165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_023317533.1"
                     /translation="MFFKRYLTEKEIYKAALPIVRLQGRVNTSLRFLSKYSDVNKNGLI
                     ALHLNLLHFISYNIHGVESLKITQAGINKAVQQSPHADEIKNIAQAIGRKGPEYIEGLI
                     RNPWGKKNDNPLIFIMSIEESIKETLNSGYMLYRVAPITEGEAAAFMDVLKQLLLFEDN
                     FRLFGRDLVYKIEKLKIR"
                     /name="hypothetical protein"
     gene            complement(10771..11097)
                     /locus_tag="K6162_RS29170"
     CDS             complement(10771..11097)
                     /locus_tag="K6162_RS29170"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_050516389.1"
                     /translation="MRERTNIMLDIGLKQSLQRLSNKTKMSLSDLINQRLSGSLSVEER
                     IGEKPEWMRNLDPYLANHFSNIELPLPSPLKEYQEKMDEIDSIINDMMVLKEYFRAKFY
                     DEIK"
                     /name="hypothetical protein"
     Typing          896..975
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="90.361"
                     /coverage="97.0"
                     /evalue="1.1100000000000001e-21"
     Typing          6492..6571
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="90.361"
                     /coverage="97.0"
                     /evalue="1.1100000000000001e-21"
     Typing          complement(313..710)
                     /name="rep_cluster_2370"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="89.526"
                     /coverage="91.0"
                     /evalue="3.03e-139"
     Typing          complement(5909..6306)
                     /name="rep_cluster_2370"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="89.526"
                     /coverage="91.0"
                     /evalue="3.03e-139"
     Typing          1562..3093
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.804"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          7158..8689
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.804"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 tgatgtgcag tgcgctgagg gcatcagtgg cgggtgtttt tccgtgggat ggatggagct
       61 ccggggataa cagaactgac gatcatttaa aggatcttct tgagatcctt tttttctgcg
      121 cgtaatctct tgcactgtaa acgaaaaaac cgcctgggga ggcggtttga tcgaaggtta
      181 agtcagttgg ggaactgctt aaccgggtaa ctgggtatac gagaccgcag ataccaaaac
      241 tgttctttca gtgtagccgc agttaggcac ccacttcaag acctctcaat atctctctcg
      301 tatatatcca gttaccaatg gctgctgcca gtggcgtttt tgcgtgtccg tccgggttgg
      361 actcaagatg atagttaccg gaaagggcgc agcagtcggg ctgaacgggg ggttcgtgca
      421 tacagcccag cttggagcga acgacctaaa ccgagccgag ataccagcag cgtgagctat
      481 gagaaagcgc cacgcttccc gaaagggaaa aaggcggaac aggtatccgg taaacggcag
      541 ggtcggaaca ggagagcgca cgagggagcc gccggagaga aatcaccggc atctttaagt
      601 cctgtcgggt ttcgtcctcc tctgacttga gcgtccattt ctgtgatgct cgtcagggga
      661 ggcggagcct atggaaaaac ggcaacgccg catccttttc tccggccctt tctgatttga
      721 gcgcctttgc tttatcccgt tatccgtgag tcggtcacgc ccgccgcagc cgaacggcag
      781 gagcgcagct accgagtgag cgaggaggcg tcatttcatc tgtccgtgca tggtattgcg
      841 gacgctcctg agccacatat cacacctgcc acatgaggca ctttcatgaa tcgtgctctc
      901 atgccaacat agtaagccag tatacacagc gtaaacgctg tgactggttc agggctgcgc
      961 cccgaaaccc gctaacacca ctgacgcgct ggcgcttgtc caacgccgga gcgacccgga
     1021 aaacttttcc gtgccgtccg gcgtttgcga aggagggata atgcagaagc cgaagaagaa
     1081 gccgctcgtc gtaaaaaaaa gatcctggca tccggcagtg atggcaaatc tgatgcgtca
     1141 ggcacaggag gaagattacc gccgggagca gatggcagag cttgagcgtt cagcccggtc
     1201 acattttgcg gatatcggag gagaggatgg cggttaagcg tacccgtttt ttaggtatcc
     1261 gggtgaccga tggggagtac cagcagctgc tggagcgctg caatggaagg caactggcgg
     1321 tgtggatgcg ggaaacctgt ctcgatacgc gcccggcgcg gtcgttgcgg ctgccgtcca
     1381 tcgatccggt tttgctgcgc cagcttgccg ggatggggaa caacctcaac cagatcgccc
     1441 ggaaaattaa cggcggccag tggtcaggcg ctgaccgggt tcaggtggtg gccgcgctga
     1501 tggccattga tgccgaactc gagcggctgc ggcatgccgt gctggaaaag ggggcagacg
     1561 atgatcgtta agtttcatcc gcgagggcga ggcggcggtg ccgggccggt ggattatctg
     1621 ctggggaaag accgccagcg cgacggggcc atcgtcctgc agggaaagcc ggaggaggtc
     1681 cgggagctta tcgacgcctc gccctacgcc aaaaagtaca cctccggcgt cctgtctttt
     1741 gctgaacagg atttaccgcc cggccagcgt gaaaagctga tggcgagctt cgagcgggtt
     1801 ctgatgcccg gactcgataa agaccagtac agcgtgctgt gggttgaaca ccgggacaag
     1861 gggcggctgg aactgaattt tctggtaccg aacacggaac tgctgaccgg caggcgtctc
     1921 cagccgtact acgaccgggc agaccgtccg cgcatcgatg cgtggcagac cgtggtgaac
     1981 gggcgactgg ggctgcacga cccgaacgca ccggagaacc ggcgcgcgct ggtgacgcca
     2041 tcttcgttac ccaaaacgaa gcaggaagcc gcagaggcta ttacgcgggg cttactggcc
     2101 ctcgcctcgt caggggagct taaaacgcgt caggacgtca ctgaggcgct ggaaagcgca
     2161 ggttttgagg tcgtgcgcac cacaaagagc agcatcagca ttgccgaccc ggacggaggg
     2221 cgaaacatcc gacttaaggg agccatctat gaacagtctt ttaacgctgg cgaaggactt
     2281 agagcagaaa tcgaaagcgc agcagcagag taccggcgaa atgctgaaag ccgcattcag
     2341 cgagcacgag aagtctgtca gagcggaact gagcgaaagc gggaagagaa tcagcgccgc
     2401 catccgcgcc cacgagcagg gtatgagcgc ggccatgcag tcgaaccgcc tgagcgtgat
     2461 gcgtatggtc gggcggacat ggctgaccat cacgatggtg tcaggactgc tgttcgccag
     2521 cctgagcggg gtgttgtggt atcaggggag cttgatagcg tcaaatctgg ccgaaatcga
     2581 ccggcagaac gcagcgctgt cgaagctgaa cgcgaagacc tggggcgtga cgtacctgga
     2641 agacagcaac ggacgttttc tggtgctgcc gaaggggacg gccgtagaca ggacgcagag
     2701 ctggacggtc gggaacgggc ggtcgaagca gaacgcgctg agactggtga aggagtaagt
     2761 gccgatgacc gagctggaaa aacagttgct gagcgcatta gagcagctac agcaggacta
     2821 ctcgaaaagg ctggacgagt gggagagcgc cttcggggaa tggcggaaaa tgtcagggct
     2881 tatgcaacgg gagaacgcgg cgctgagcga gcgcgtgacg cgcttgagtc agcaggtgga
     2941 acgcttgagc gggcaactgc agcgtttgag ccggtagtgc agcgtcatga gatggccgtg
     3001 gccgcagaac gggcgcacga gcaacggcag cacgaaaagg aactgacgga agcgcgttcc
     3061 cggcagcgaa gctatgacgg tccgtcgctg ggctgaaatg caggaaaaca gggttctcat
     3121 cgggcattta cgctgaaacc ggatgtcatt cacctggctg catggctatg cagccaggta
     3181 aaatttatct gtcgcgaacg gggcggttta tcgggtggtt tgttgccggt tttacctgac
     3241 tgccaccccg ttcgcggcga acccgtccgg ggcggtgcgg gcaacgggtg ttatgtttaa
     3301 tcagcgtgaa cggcagatac aaaaaagccc cggagggctt ttaactactg acttattttt
     3361 ttgaagattt gctgggtttc aatttgtcag tatttcccgg cgttttttta ttgtcacgct
     3421 ggcgtttatc aggttcccct gttgaatcgg caattcgttt cggacccggc ttaagaccca
     3481 taatcgttat cctttcaata gttaagagga aagccctcaa gctagatata ttgaccgcgt
     3541 tacggattat caagaggggc taacaagatt attttttgtt cttttaggtt gattgtggta
     3601 ctgttgatat acatgtatag caaatggtgt tcgggaggtt tatatgcttg ctatccggtt
     3661 atctgatgag attgagtccc gtctggactc gctggcgaag caaaccggca gaacaaagac
     3721 gttttatgcg cgggaagcaa tactggcgca tctggaagac ctggaggatt attatctttc
     3781 agcagaaact gctgcacgcg ttcgccgtgg tgatgaagca gtgcattcgt ctgaagacgt
     3841 gaggaagtca cttggtctgg acgattaact attccgatcg ggcgctcaaa tcgttacgca
     3901 agatggacaa acagaacgca cgacggattg tggattttat gagtttacgc attgcagttg
     3961 ctgccgatcc tcgccagtca gggaagccgc tcaaaggtga gctgggcgag ttctggcgct
     4021 atcgggtggg agattatcgc gttctgtgtg agatccgaga tgacgagctt gttatccttg
     4081 ccgccacgat tggacatcgc cgcgaagttt acgactgaag cccgctatgc gggctttttt
     4141 gatgccgaca acaggtatta tggtaaatac tcgactaact acgtgttcta ctgtttgtcg
     4201 gttttagtta accagcaggt tgtcttaacg ataatgtagc cgttattttt aactaataag
     4261 gtcatattgt taacgtcagg cataataaaa gacattatgg cctcagagtt atcatcagaa
     4321 gcagctctct gcattactac ttcgattccc ttttctttga atctataagt atccagttgc
     4381 ctattaaact tagaaagacc tacaaccata cttagtttgt tatctttaac agtaagagat
     4441 gcttcctgag taccattaca tcggtagtaa gcggtgtaac cgtcagcaaa agatcgaccg
     4501 gggaatataa atgctaggaa cgcaattaac caatacatag ttgagaatct tggtattaca
     4561 catttcacta tcctctcttt catttttcgt tcaccatact ttgttatctg atttttaatt
     4621 tttctatttt ataaaccaag tctctaccaa aaaggcgaaa attatcctca aataataata
     4681 attgtttaag aacatccatg aaagcagctg cttcaccctc agttatggga gctactctat
     4741 ataacatata acctgagtta agggtttctt ttatactttc ttcaatgctc attataaata
     4801 ttagtggatt atcatttttc ttcccccatg ggtttctaat taatccttcg atgtactcag
     4861 gtcctttgcg tcctattgcc tgcgcaatat tttttatttc atctgcgtga gggctttgtt
     4921 gtacagcttt gtttatgcct gcttgagtga ttttcagaga ctctacaccg tgtatgttgt
     4981 atgagataaa atgtaataaa ttaagatgca atgcaattaa tccatttttg tttacatctg
     5041 agtatttgga taaaaaacgt aatgaagtgt taactctccc ttgaagccta acaattggga
     5101 gtgctgcctt gtatatttcc ttctccgtta aataccgttt aaaaaacata attctccgcc
     5161 tccgtatagc gtgcctattt tatttcgtcg taaaacttag ctctgaaata ttcctttaga
     5221 accatcatgt cattaataat tgaatctatc tcatccattt tttcttggta ttcttttaat
     5281 ggtgaaggga gcggtaactc aatatttgaa aagtgattgg cgagataggg atcaaggttt
     5341 ctcatccatt caggcttttc gccaatgcgt tcttcaactg aaagtgaacc tgataatctc
     5401 tggttaataa gatcacttag actcattttg gttttattcg aaagccgttg taaggattgc
     5461 tttaatccta tatctagcat gatatttgtt ctttctctca ttaccccccc ttgctattgc
     5521 gttttttgtt gcgcacaaag ctaccattgc gcattaagaa acgcaataaa aaaggaggga
     5581 gggagtggtt tgtacatgat gtgcagtgcg ctgagggcat cagtggcggg tgtttttccg
     5641 tgggatggat ggagctccgg ggataacaga actgacgatc atttaaagga tcttcttgag
     5701 atcctttttt tctgcgcgta atctcttgca ctgtaaacga aaaaaccgcc tggggaggcg
     5761 gtttgatcga aggttaagtc agttggggaa ctgcttaacc gggtaactgg gtatacgaga
     5821 ccgcagatac caaaactgtt ctttcagtgt agccgcagtt aggcacccac ttcaagacct
     5881 ctcaatatct ctctcgtata tatccagtta ccaatggctg ctgccagtgg cgtttttgcg
     5941 tgtccgtccg ggttggactc aagatgatag ttaccggaaa gggcgcagca gtcgggctga
     6001 acggggggtt cgtgcataca gcccagcttg gagcgaacga cctaaaccga gccgagatac
     6061 cagcagcgtg agctatgaga aagcgccacg cttcccgaaa gggaaaaagg cggaacaggt
     6121 atccggtaaa cggcagggtc ggaacaggag agcgcacgag ggagccgccg gagagaaatc
     6181 accggcatct ttaagtcctg tcgggtttcg tcctcctctg acttgagcgt ccatttctgt
     6241 gatgctcgtc aggggaggcg gagcctatgg aaaaacggca acgccgcatc cttttctccg
     6301 gccctttctg atttgagcgc ctttgcttta tcccgttatc cgtgagtcgg tcacgcccgc
     6361 cgcagccgaa cggcaggagc gcagctaccg agtgagcgag gaggcgtcat ttcatctgtc
     6421 cgtgcatggt attgcggacg ctcctgagcc acatatcaca cctgccacat gaggcacttt
     6481 catgaatcgt gctctcatgc caacatagta agccagtata cacagcgtaa acgctgtgac
     6541 tggttcaggg ctgcgccccg aaacccgcta acaccactga cgcgctggcg cttgtccaac
     6601 gccggagcga cccggaaaac ttttccgtgc cgtccggcgt ttgcgaagga gggataatgc
     6661 agaagccgaa gaagaagccg ctcgtcgtaa aaaaaagatc ctggcatccg gcagtgatgg
     6721 caaatctgat gcgtcaggca caggaggaag attaccgccg ggagcagatg gcagagcttg
     6781 agcgttcagc ccggtcacat tttgcggata tcggaggaga ggatggcggt taagcgtacc
     6841 cgttttttag gtatccgggt gaccgatggg gagtaccagc agctgctgga gcgctgcaat
     6901 ggaaggcaac tggcggtgtg gatgcgggaa acctgtctcg atacgcgccc ggcgcggtcg
     6961 ttgcggctgc cgtccatcga tccggttttg ctgcgccagc ttgccgggat ggggaacaac
     7021 ctcaaccaga tcgcccggaa aattaacggc ggccagtggt caggcgctga ccgggttcag
     7081 gtggtggccg cgctgatggc cattgatgcc gaactcgagc ggctgcggca tgccgtgctg
     7141 gaaaaggggg cagacgatga tcgttaagtt tcatccgcga gggcgaggcg gcggtgccgg
     7201 gccggtggat tatctgctgg ggaaagaccg ccagcgcgac ggggccatcg tcctgcaggg
     7261 aaagccggag gaggtccggg agcttatcga cgcctcgccc tacgccaaaa agtacacctc
     7321 cggcgtcctg tcttttgctg aacaggattt accgcccggc cagcgtgaaa agctgatggc
     7381 gagcttcgag cgggttctga tgcccggact cgataaagac cagtacagcg tgctgtgggt
     7441 tgaacaccgg gacaaggggc ggctggaact gaattttctg gtaccgaaca cggaactgct
     7501 gaccggcagg cgtctccagc cgtactacga ccgggcagac cgtccgcgca tcgatgcgtg
     7561 gcagaccgtg gtgaacgggc gactggggct gcacgacccg aacgcaccgg agaaccggcg
     7621 cgcgctggtg acgccatctt cgttacccaa aacgaagcag gaagccgcag aggctattac
     7681 gcggggctta ctggccctcg cctcgtcagg ggagcttaaa acgcgtcagg acgtcactga
     7741 ggcgctggaa agcgcaggtt ttgaggtcgt gcgcaccaca aagagcagca tcagcattgc
     7801 cgacccggac ggagggcgaa acatccgact taagggagcc atctatgaac agtcttttaa
     7861 cgctggcgaa ggacttagag cagaaatcga aagcgcagca gcagagtacc ggcgaaatgc
     7921 tgaaagccgc attcagcgag cacgagaagt ctgtcagagc ggaactgagc gaaagcggga
     7981 agagaatcag cgccgccatc cgcgcccacg agcagggtat gagcgcggcc atgcagtcga
     8041 accgcctgag cgtgatgcgt atggtcgggc ggacatggct gaccatcacg atggtgtcag
     8101 gactgctgtt cgccagcctg agcggggtgt tgtggtatca ggggagcttg atagcgtcaa
     8161 atctggccga aatcgaccgg cagaacgcag cgctgtcgaa gctgaacgcg aagacctggg
     8221 gcgtgacgta cctggaagac agcaacggac gttttctggt gctgccgaag gggacggccg
     8281 tagacaggac gcagagctgg acggtcggga acgggcggtc gaagcagaac gcgctgagac
     8341 tggtgaagga gtaagtgccg atgaccgagc tggaaaaaca gttgctgagc gcattagagc
     8401 agctacagca ggactactcg aaaaggctgg acgagtggga gagcgccttc ggggaatggc
     8461 ggaaaatgtc agggcttatg caacgggaga acgcggcgct gagcgagcgc gtgacgcgct
     8521 tgagtcagca ggtggaacgc ttgagcgggc aactgcagcg tttgagccgg tagtgcagcg
     8581 tcatgagatg gccgtggccg cagaacgggc gcacgagcaa cggcagcacg aaaaggaact
     8641 gacggaagcg cgttcccggc agcgaagcta tgacggtccg tcgctgggct gaaatgcagg
     8701 aaaacagggt tctcatcggg catttacgct gaaaccggat gtcattcacc tggctgcatg
     8761 gctatgcagc caggtaaaat ttatctgtcg cgaacggggc ggtttatcgg gtggtttgtt
     8821 gccggtttta cctgactgcc accccgttcg cggcgaaccc gtccggggcg gtgcgggcaa
     8881 cgggtgttat gtttaatcag cgtgaacggc agatacaaaa aagccccgga gggcttttaa
     8941 ctactgactt atttttttga agatttgctg ggtttcaatt tgtcagtatt tcccggcgtt
     9001 tttttattgt cacgctggcg tttatcaggt tcccctgttg aatcggcaat tcgtttcgga
     9061 cccggcttaa gacccataat cgttatcctt tcaatagtta agaggaaagc cctcaagcta
     9121 gatatattga ccgcgttacg gattatcaag aggggctaac aagattattt tttgttcttt
     9181 taggttgatt gtggtactgt tgatatacat gtatagcaaa tggtgttcgg gaggtttata
     9241 tgcttgctat ccggttatct gatgagattg agtcccgtct ggactcgctg gcgaagcaaa
     9301 ccggcagaac aaagacgttt tatgcgcggg aagcaatact ggcgcatctg gaagacctgg
     9361 aggattatta tctttcagca gaaactgctg cacgcgttcg ccgtggtgat gaagcagtgc
     9421 attcgtctga agacgtgagg aagtcacttg gtctggacga ttaactattc cgatcgggcg
     9481 ctcaaatcgt tacgcaagat ggacaaacag aacgcacgac ggattgtgga ttttatgagt
     9541 ttacgcattg cagttgctgc cgatcctcgc cagtcaggga agccgctcaa aggtgagctg
     9601 ggcgagttct ggcgctatcg ggtgggagat tatcgcgttc tgtgtgagat ccgagatgac
     9661 gagcttgtta tccttgccgc cacgattgga catcgccgcg aagtttacga ctgaagcccg
     9721 ctatgcgggc ttttttgatg ccgacaacag gtattatggt aaatactcga ctaactacgt
     9781 gttctactgt ttgtcggttt tagttaacca gcaggttgtc ttaacgataa tgtagccgtt
     9841 atttttaact aataaggtca tattgttaac gtcaggcata ataaaagaca ttatggcctc
     9901 agagttatca tcagaagcag ctctctgcat tactacttcg attccctttt ctttgaatct
     9961 ataagtatcc agttgcctat taaacttaga aagacctaca accatactta gtttgttatc
    10021 tttaacagta agagatgctt cctgagtacc attacatcgg tagtaagcgg tgtaaccgtc
    10081 agcaaaagat cgaccgggga atataaatgc taggaacgca attaaccaat acatagttga
    10141 gaatcttggt attacacatt tcactatcct ctctttcatt tttcgttcac catactttgt
    10201 tatctgattt ttaatttttc tattttataa accaagtctc taccaaaaag gcgaaaatta
    10261 tcctcaaata ataataattg tttaagaaca tccatgaaag cagctgcttc accctcagtt
    10321 atgggagcta ctctatataa catataacct gagttaaggg tttcttttat actttcttca
    10381 atgctcatta taaatattag tggattatca tttttcttcc cccatgggtt tctaattaat
    10441 ccttcgatgt actcaggtcc tttgcgtcct attgcctgcg caatattttt tatttcatct
    10501 gcgtgagggc tttgttgtac agctttgttt atgcctgctt gagtgatttt cagagactct
    10561 acaccgtgta tgttgtatga gataaaatgt aataaattaa gatgcaatgc aattaatcca
    10621 tttttgttta catctgagta tttggataaa aaacgtaatg aagtgttaac tctcccttga
    10681 agcctaacaa ttgggagtgc tgccttgtat atttccttct ccgttaaata ccgtttaaaa
    10741 aacataattc tccgcctccg tatagcgtgc ctattttatt tcgtcgtaaa acttagctct
    10801 gaaatattcc tttagaacca tcatgtcatt aataattgaa tctatctcat ccattttttc
    10861 ttggtattct tttaatggtg aagggagcgg taactcaata tttgaaaagt gattggcgag
    10921 atagggatca aggtttctca tccattcagg cttttcgcca atgcgttctt caactgaaag
    10981 tgaacctgat aatctctggt taataagatc acttagactc attttggttt tattcgaaag
    11041 ccgttgtaag gattgcttta atcctatatc tagcatgata tttgttcttt ctctcattac
    11101 ccccccttgc tattgcgttt tttgttgcgc acaaagctac cattgcgcat taagaaacgc
    11161 aataaaaaag gagggaggga gtggtttgta ca
//
