LOCUS       NZ_CP082013            11959 bp    DNA     circular CON 10-JAN-2024
DEFINITION  Klebsiella pneumoniae strain KP18-238 plasmid pKP18-238-3, complete
            sequence.
ACCESSION   NZ_CP082013
VERSION     NZ_CP082013.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN20928632
            Assembly: GCF_019823855.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 11959)
  AUTHORS   Qin,S. and Dong,H.
  TITLE     Klebsiella pneumoniae strains
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11959)
  AUTHORS   Qin,S. and Dong,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2021) Institute of Drug Discovery & Development,
            Zhengzhou University, 100 Science Avenue, Zhengzhou City, Zhengzhou,
            Henan 450001, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: unicycler v. v.0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 12x
            Sequencing Technology             :: Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/10/2024 03:26:04
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,882
            CDSs (total)                      :: 5,759
            Genes (coding)                    :: 5,600
            CDSs (with protein)               :: 5,600
            Genes (RNA)                       :: 123
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 61 of 159
            Pseudo Genes (incomplete)         :: 106 of 159
            Pseudo Genes (internal stop)      :: 14 of 159
            Pseudo Genes (multiple problems)  :: 22 of 159
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP082013.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..11959
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="KP18-238"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pKP18-238-3"
                     /geo_loc_name="China"
                     /lat_lon="34.73 N 113.70 E"
                     /collection_date="2018-10-04"
                     /collected_by="Zhengzhou University"
     gene            55..333
                     /locus_tag="K6162_RS00005"
     CDS             55..333
                     /locus_tag="K6162_RS00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014542884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family ribbon-helix-helix protein"
                     /protein_id="WP_000093040.1"
                     /translation="MSSATSIRLDEELKDRLKTLADDRHRSAHALMLEAITEYIDREEK
                     RSQYLRDGQAAWQHYQETGLHLTAEEAEAWISTWGTENEQDAPPCHR"
                     /name="CopG family ribbon-helix-helix protein"
     gene            321..635
                     /locus_tag="K6162_RS00010"
     CDS             321..635
                     /locus_tag="K6162_RS00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001136729.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]; GO:0009432 - SOS response [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_071177730.1"
                     /translation="MPQVTISALAQRDLQRLQDFLKTKNRLAARKGGEVIVRAIQQLKT
                     LPDIGRPVPFLPLEYKELVIGFGDSGYVMLYRHDREMDQIVIVTVRHQKESGYPGADSL
                     "
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            799..1227
                     /locus_tag="K6162_RS00015"
     CDS             799..1227
                     /locus_tag="K6162_RS00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001140599.1"
                     /translation="MAKTLLNLSQAAQAAGITRRTLYNHVKQGKVTVSRDGKNNPVVDV
                     SELIRVYGNVNIPEKQIPGISHRENTQKNFPQEQLLAMQKELADLRQAVTLMLEDKTSR
                     EEERRQHDDERRKLQAEVDRLTTELTQKKKRFWSGWFS"
                     /name="hypothetical protein"
     gene            1253..1432
                     /locus_tag="K6162_RS00020"
     CDS             1253..1432
                     /locus_tag="K6162_RS00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165970.1"
                     /translation="MTKQEKTALNMAKFIKDQSLLLLEKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(1459..1989)
                     /locus_tag="K6162_RS00025"
     CDS             complement(1459..1989)
                     /locus_tag="K6162_RS00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_071177729.1"
                     /translation="MALYMVFIEDVEDKDGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGENPQWERDKPTGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQINAMIEVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(1996..2727)
                     /gene="mobC"
                     /locus_tag="K6162_RS00030"
     CDS             complement(1996..2727)
                     /gene="mobC"
                     /locus_tag="K6162_RS00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WP_071177728.1"
                     /translation="MTLEQYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRAKYPVDGRQVSVWGLTPHGVAFSFDEDEPLTDVIPFQP
                     SRVSAAQLPHRLAVQSLRLSMEGRGATGWRYLHRMALKGMKVPDALAELDGRTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRRANGIDEIWYICPDRPTLVRVQRAILAVDEIVNPQTGEA
                     RKTVELDRERLFACFKFMTTE"
                     /name="mobC"
     gene            complement(2727..4691)
                     /locus_tag="K6162_RS00035"
     CDS             complement(2727..4691)
                     /locus_tag="K6162_RS00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_071177727.1"
                     /translation="MFNTDSLAWQWHIPELARQIYPWVSWLYREYYSLAGFFLVIGLFF
                     GLAELWAGRMRFVGLSHKARREYADRYKRDNNRTVPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLV
                     AGVLLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSTEQARKQQAVFLGKDEQGQPVLVPRDTWRKTNIQILGLPGSGKSAMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLV
                     AGFNLSETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     KLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFRRWPHASIMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPADFV
                     KTTVLDNTPIRWFYRAASQESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARLAFSSPLRVERREISLKTFPVLAKTDPLAEY
                     LPPADGGGPKADDDFAGLY"
                     /name="TraM recognition domain-containing protein"
     gene            5823..5981
                     /locus_tag="K6162_RS29555"
     CDS             5823..5981
                     /locus_tag="K6162_RS29555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071596352.1"
                     /translation="MHEPPVQPDCCALSGNYHLESNPDGHAKTPLAAAIGNWIYTREIL
                     RGLEVGA"
                     /name="replication initiation protein"
     gene            complement(6164..6313)
                     /locus_tag="K6162_RS00040"
     CDS             complement(6164..6313)
                     /locus_tag="K6162_RS00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_032440454.1"
                     /translation="MKKVKTIFLFILIVAGFLLAACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     gene            complement(6398..6655)
                     /locus_tag="K6162_RS00045"
     CDS             complement(6398..6655)
                     /locus_tag="K6162_RS00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /GO_function="GO:0015643 - toxic substance binding
                     [Evidence IEA]"
                     /GO_process="GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E3-like toxin immunity protein"
                     /protein_id="WP_032440455.1"
                     /translation="MGLKLRLTWFDKKTEEFKGEEYSKDFGDDGSVIESLGMPLKDNIN
                     NGEFDLKKEWVPLLQPFFKNQIKPDVDNCYIAFDYRDGNW"
                     /name="colicin E3-like toxin immunity protein"
     gene            complement(6665..8350)
                     /locus_tag="K6162_RS00050"
     CDS             complement(6665..8350)
                     /locus_tag="K6162_RS00050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase domain-containing
                     protein"
                     /protein_id="WP_032440457.1"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSVSGDALSAAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPKMMSK
                     IMTSLPADAVTETPASTLPLDQATVRVRQRVVDVVKDERQHIAVVAGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPSLQVSVPKGVPTAKAPPKGIVAEKGDSRPAGFTAGGNSREAVIR
                     FPKETGQKPVYVSVTDVLTPAQVKQRQEEEKRRQQAWDAAHPEEGLKRDYDKAKAELDA
                     EDKNIATLNSRIASTEKALPGARAAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNEKLKAAQASLNAMNESLSRDKAALTGAMESRKQKEKKAKDAENKLNE
                     EKNKPRKGTKDYGHDYHPVPKTEDIKGLGELKRGDPKTPKQGGGGKRARWYGDKKRKIY
                     EWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPKRNIKKYL"
                     /name="colicin-like bacteriocin tRNase domain-containing
                     protein"
     gene            complement(8455..9942)
                     /gene="ltrA"
                     /locus_tag="K6162_RS00055"
     CDS             complement(8455..9942)
                     /gene="ltrA"
                     /locus_tag="K6162_RS00055"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226014.1"
                     /GO_function="GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="WP_004178082.1"
                     /translation="MMYGEEKSDSLIVAAKQANNPKGAESVERRSGAKGNAEQPHMRRT
                     QSRESMSQRLSRVREAAKQRKKERFTALFHLLTVEALEAAFLSLSRKAAAGVDGIRWMD
                     YAGNMKNNITDLHRRLHQGSYRAQPGRRHYIPKADGKQRPLGIASLEDKIVQYALVKIL
                     NAVYENDFMGFSYGFRPGRSQHDALDALATGLVRTNVNWVLDADISQFFDRVSHEWLIR
                     FTEHRIGDRRVIRLIRKWLTAGTSEEGQWRATEEGTPQGAVISPLLANIYLHYVFDLWA
                     HQWRRRYATGNVVMVRYADDIVIGFDKRYDARRFRIAMQRRLREFGLTVHPEKTRLMEF
                     GRFAAENRAIRGKGKPETFNFLGFTHISGKDRNGRFMLIRKTRRDRMTATLKAIKDGLR
                     RRWHYSIPEQGKWLRRVVQGYLNYHSVPGNFPTMQKFRTHVTNLWRRALRRRSQKDDTT
                     WTKANKLAAAWLPRVRVLHPWPVERFTARHPRQEPGA"
                     /name="ltrA"
     gene            10587..10832
                     /locus_tag="K6162_RS00060"
     CDS             10587..10832
                     /locus_tag="K6162_RS00060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032440458.1"
                     /translation="MTHLKLTPAEEKRLADAFVAQALAMGLKGNKTLPKAVKQQVRERV
                     LRAIQDEREARRAQEMRREKYEATETTFTWQPQRRR"
                     /name="hypothetical protein"
     gene            11106..11477
                     /gene="tolA"
                     /locus_tag="K6162_RS00065"
     CDS             11106..11477
                     /gene="tolA"
                     /locus_tag="K6162_RS00065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237044.1"
                     /GO_component="GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA]"
                     /GO_function="GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="WP_001237044.1"
                     /translation="MRLIPFILLALCSPLLLTACKHSTTQPPKIYTLDKSSKISSYAAA
                     IKTAVEMQMFEPEAYAGKMCTVRISIQRDGTLNSAIAEGGDPKLCKAAISAVTHAKIPP
                     APDENTWQIFKNAPLDFRP"
                     /name="tolA"
     gene            11474..11839
                     /locus_tag="K6162_RS29175"
     CDS             11474..11839
                     /locus_tag="K6162_RS29175"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /GO_function="GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="WP_072354022.1"
                     /translation="MKAFTLTVLAAMFLTPVHAIPLKPVTTLQPTYPVQAAAKRIEGQV
                     TATFDILDDGRVDNVSLTGCHLFFRETLTAMRHWLYPAGHPAMRQVINFEFSLKNDGLQ
                     DPRPVTTQKLNDDGCRQ"
                     /name="TonB family protein"
     BGC             1665..11959
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          5494..6061
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="88.795"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(2000..2727)
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.416"
                     /coverage="100.0"
                     /evalue="1.35e-140"
ORIGIN
        1 ccgccacgga ttaaagtagt catgtgtaat acatatgact acaaaggggc ttttatgtca
       61 tcagcaacat ccatcaggct cgatgaagaa cttaaggacc ggctgaaaac gctggccgat
      121 gaccggcacc gctcagcgca cgcgctaatg ctggaggcaa tcaccgaata catcgaccgg
      181 gaagagaagc gcagccagta cctgcgggac ggacaggccg catggcagca ctatcaggaa
      241 acgggtctgc accttactgc ggaagaagcg gaagcctgga taagtacctg gggaactgag
      301 aacgagcagg atgctccgcc atgccacagg taacgatttc cgcgctggca cagcgcgatt
      361 tacaacgcct tcaggacttt ctcaaaacca aaaatcggct ggcagccaga aaaggaggtg
      421 aggtgatcgt ccgggctatc cagcaactga aaacattgcc agacattggc cgcccggtgc
      481 cgtttctgcc gctggaatat aaggaactgg tgatcgggtt tggcgacagt ggctatgtga
      541 tgctctaccg ccacgaccgg gaaatggacc agattgtgat cgtcaccgtc aggcatcaga
      601 aagaatccgg gtatccgggg gcagacagcc tctaataacc gatgacgggg gcgcaattca
      661 ttgcgtggcc gtcgccggtt tgacaagcgc agcgcgtcag aaaaccacat cctgaacgga
      721 aaaacgggta aaattcagcg atacatttac cgtgagaaat tacacacctt tcacacccga
      781 taaaatcagg ggtatacagt ggcgaaaaca ttgctcaacc tttcacaggc agcacaagcc
      841 gcgggcatta ccagaagaac gctatacaat catgtaaaac agggaaaggt taccgtgtca
      901 cgtgacggga aaaacaaccc tgtcgtggat gtgtcagaac tgatccgtgt atacggaaat
      961 gtgaatatcc ctgagaaaca aattcccggc atttcacacc gtgaaaatac acaaaaaaat
     1021 ttcccacagg aacagcttct ggcaatgcaa aaggaactgg ccgatctccg gcaggctgtc
     1081 accctgatgc tggaagataa aacatcccgg gaagaagaac gccgccagca cgatgatgag
     1141 cgccggaaac tccaggccga agtggacaga ttgacaacag aactgacaca gaagaaaaaa
     1201 cgcttctggt ccggctggtt tagctgagaa cttaccgaag ggaatggaga acatgacgaa
     1261 gcaggaaaaa actgccctca atatggctaa attcattaag gatcagtcgc tgctgttgct
     1321 ggaaaagctg aacgagctgg acatggatac cccggccgac atgtgcgaac ggcttcacga
     1381 ggatgcggaa acactctggc aggtgatgcg ggataagctc ggggaagaat aacagcaccg
     1441 gccatgccgg tgctggcgtt attttttgcc gaacggccac caccggcggg cgggtttgtc
     1501 ctccggcagt tcaggcaccg gcacgttaaa atagcggctg ttatattccc gcatcagctc
     1561 cgctttcagc ttctcgttca ccgcgctttc cccgtgccgc tcgctgacca cctcaatcat
     1621 cgcgttaatc tgctccggtg agcacttccc ggcatcaacc acctgcacac cgttctcgat
     1681 ggcgaaccgc tccacctttc gatagaggta atcacacagc ttcattcctg tgggtttgtc
     1741 ccgttcccat tgcggatttt caccggtgaa aaagtacgtg gctttttcgt cgctgttgag
     1801 ccgctcgtca tacatcccga tggatttcat catcgccaga tgctgggccc ggtcgacaaa
     1861 acagaaaaca tacgccccgg tctgccaggc gagcaccacc atgtcttcca gatgctcgct
     1921 ggttggcata atgcagcgct cgccgtcttt gtcttccacg tcctcaataa ataccatgta
     1981 cagcgccata atccctcact ccgttgtcat aaatttaaag cacgcgaaca gcctctcccg
     2041 gtccagctcc accgttttgc gggcctcgcc ggtctgtgga ttcacgattt catccaccgc
     2101 cagaatcgcc cgctgtaccc ggacaagcgt cggccggtca gggcagatgt accagatttc
     2161 atcgatgccg ttcgcccggc ggttgaacag atacccggac accacttcct gataccgcct
     2221 gcggctcttg acggtgcgct ctacctcaaa tgccaccgtt ctgccgtcca gctccgccag
     2281 cgcgtccggc actttcatcc ctttcagggc catacggtgc aggtaccgcc agccggtggc
     2341 cccccggccc tccatggaga ggcgcaggga ctgcacggcc agccggtgcg gcagctgcgc
     2401 cgccgacacc cttgacggct gaaacggaat aacgtccgtc agcggctcgt cctcgtcgaa
     2461 cgagaacgcc accccgtgcg gcgtcagccc ccacacgctg acctgacggc cgtcaaccgg
     2521 gtatttcgcc cggaccagca gccctttcgc caccagactg tccaggaagc ggtaaaccgg
     2581 ctgtaccgcc gcaatcccca gccgtcgcgc aaccagctcc gccgtggaac acagctcttc
     2641 ccgcagccag cgcagggtca gccgtgtttt ctccgcgttc cgcgcctgcc gcttcgcgtg
     2701 gctgacgttg tactgctcca gtgtcatcag taaagccccg caaaatcatc atccgctttt
     2761 ggtccgccgc cgtcggccgg aggcaggtac tccgccagcg ggtccgtttt cgccagcacc
     2821 gggaaggttt tcagcgaaat ttcccggcgc tcaacccgca gcgggctgct gaacgccagc
     2881 cgcgccacgc ccagcccggt catcaccgcg aacccgtcag gcaggtgttg cagggtgttc
     2941 acgtcaaaca gcggccgcgc ctccttctgg atgaagctgt cgccgctgat gtgctccacg
     3001 ttccccgcct cccggctggc ccggcggcgc tccacgtcca cccggatctc cccggtctgg
     3061 cccgccgccc actgcgcgga ctcctggctg gccgcgcgat agaaccagcg gatcggcgtg
     3121 ttatccagta ccgtggtttt cacaaaatca gcgggcagat cctgcccgca ctgaccgaag
     3181 tcgcccagcg actggtgcgc gagcagcagg ttgcagtccc ggctgcgcag cgtccccagc
     3241 gcgttcagca cgtacttcga cagcagatat ttaatctcat cgagcatgat gctcgcgtgc
     3301 ggccacctcc ggaactcatc ccgcgcaatg atgatttgcg cgcagcgggc aaacagcatc
     3361 ttctgcaccc ggatcaccgc ctcatcgtcc atcgagccga tgacgtacag gcagccgccg
     3421 ccgttgatta tccccgccac atcgataccg ctgtccgtct gcaggacgct caggtcggcc
     3481 acgttctcca gcttggtgat cagccccttc acgtcctttt tcagggcctc cggcaacgcg
     3541 tacgccgccg ccagcacctg ctgaatgttt gccccctgcg ggaactgctc cgcaatcagc
     3601 ttcgccgctt tctgctcgct gatgcggtag tggtccgcca cgtcgccggt ctcactcagg
     3661 ttaaacccgg caaccagcag attcttcagg gcgtactggt cgagatcgcg gaacagattc
     3721 agctgcgccg gtttccccgg ccgcagatcc agcagggtga agtccgggca gtgcgcccgg
     3781 aagacgtgcg gcgcccacgc gtcccctttg gggtcgaagt acaccacagc gtccccgaaa
     3841 tcgcgtacgc agcgaatgag cgcgttggtg gccatcgcgc tcttgccgct cccgggcagg
     3901 ccgaggatct gaatattggt cttgcgccag gtatcacgag ggaccagcac cggctggccc
     3961 tgctcatcct tacccagaaa caccgcctgc tgtttccggg cctgctccgt ggaaaaatcg
     4021 gccagcgcgt taaccggtac cggctccccg aaatgcaggt ggcggacgtc tgtcatttct
     4081 ccggtccgct ggctgctgcg ccggctctcc tgattcagtt tcgcgttcac cgcctcgtca
     4141 gcggccgcaa aatgaccatc aagcagcagg aacgccagca gccgcgccag aaggacgccg
     4201 gcgacaagcc ctccggcaca ccaggctatc ccgaacaggt tagccggctg cagcaggtaa
     4261 taatctgcgt tcgcatagag gtccgggcgc aggacggttt ctccccagct ctcgataaac
     4321 acggcgacaa ccagcccccc cagaagggcc agaccggcag cgccggtcag ccagtacgcg
     4381 tagttccaga catgtcgccg tgccctgacc atccgcccgg gtgaatccag cgaggccagc
     4441 accgaaaatg gcacagtccg gttattatcg cgcttatagc gatcggcata ctcccgccgc
     4501 gccttatggc tcagccccac aaaacgcatt cggccggccc agagttcagc cagaccgaaa
     4561 aacaggccga taaccagaaa aaaaccggcc agactgtaat actcccggta cagccagctg
     4621 acccacgggt aaatctgccg ggccagctcc gggatatgcc actgccaggc aagcgaatcc
     4681 gtattaaaca tgcaatctct ctccttcaga cgacgggttt accggcgtga tgcgcagccc
     4741 ccgctgcaca agccgcggac atgccccaag gcatggcggc ggcggtcacg tccgtggtga
     4801 ttgcgttcag ctttcaccgc ggaggccagc ctctttccgc cccatgcgga aaaggcgcag
     4861 ccccggagga acaacgagca cgccccagcg tgtgagagga aaaggcggca ccccagtgcg
     4921 gtcttttccg gtgttccggc ccccgtcaga gttcgccata gcgagcggct gaccacccgc
     4981 aaaatcagga gcagctttgc tgcttcgtca ggtgcgtcca gcaccgtgga tttttgtggg
     5041 tggtcaggcg tgagcctgac gggggccggg tgaatttctg gcgtggccgg cgtccagccg
     5101 gacagagcca gaaagagggg aaaagaccgt gcgtcggtgg cgtccagcca gcgtaagcaa
     5161 ggtcttttcc cctgaagtca gccacgcccc agcggggatg acttgcattt ctccgcaagg
     5221 agaaaaaagc aacagagata ggttaacgta aaatgttcag cgaaaaaccg acatggttca
     5281 cctatcctga taattgatcg tcagggaata gaaagactta atcaggggac aatatcccac
     5341 atcagcgcgt ccggtgaccg tcttgcaggt gccggggaaa ttgcgcctcc tcgctcactc
     5401 ggtcgctacg ctccgggcgt gagactgcgg cgggcgctgc ggacacatac aaagttaccc
     5461 acagattccg tggataagca ggggactaac atgtgaggca aaacggcagg gccgcgccgg
     5521 tggcgttttt ccataggctc cgccctcctg ccagagttca cataaacaga cgctttttcg
     5581 gtgcatctgt gggagccgtg aggctcaacc atgaatctga cagtacgggc gaaacccgac
     5641 aggacttaaa gatccccacc gtttccggta ggtcgctccc tcttgcgctc tcctgttccg
     5701 accctgccgt ttaccggata cctgttccgc ctttttccct ttcgggaagc gtggcgcttt
     5761 ctcatagctc acgctgctgg tatctcggct cggtttaggt cgttcgctcc aagctgggct
     5821 gtatgcacga accccccgtt cagcccgact gctgcgccct ttccggtaac tatcatcttg
     5881 agtccaaccc ggacggacac gcaaaaacgc cactggcagc agccattggt aactggatat
     5941 atacgagaga gatattgaga ggtcttgaag tgggtgccta actgcggcta cactgaaaga
     6001 acagttttgg tatctgcggt ctcgtatacc cagttacccg gttaagcagt tccccaactg
     6061 acttaacctt cgatcaaacc gcctccccag gcggtttttt cgtttacagt gcaagagatt
     6121 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctactga accgcgatcc
     6181 ccgtcagttc agaggaggac gatggtgcca ccgttccgcc ctgaacatcc cggatataat
     6241 ttgcctgaca tgcggccaga agaaagccag caacaatcaa tataaaaaga aaaatagtct
     6301 ttaccttttt catatacttt ccctgtattt aaagactccg gcattcatcc ctgaacaccg
     6361 gaagtcagta tttcaaatca tccttgataa ttttttgtta ccagttaccg tcccgataat
     6421 caaaagctat ataacaatta tccacatcag gttttatttg atttttaaaa aatggctgta
     6481 gtaaaggaac ccattctttc ttcaaatcaa actcaccatt attaatatta tcctttaaag
     6541 gcattcccaa gctttcaatg acagaaccat cgtcaccaaa gtctttagaa tactcttccc
     6601 ctttaaactc ttcagttttc ttatcaaacc atgtcaaacg taatttaagc cccatattta
     6661 cctctcaaag atatttttta atgttccgtt ttggatctgg ccctttaacc tgcttacccg
     6721 ttttgggatc aaatgcgcct aggtgttcgc catcactggc acgataccct tcaagctcac
     6781 cgtgctggga atcccattca taaattttac gttttttatc gccataccat cgggcccgct
     6841 taccaccacc accttgttta ggtgttttag gatcacctcg ctttaactct ccaagtccct
     6901 ttatgtcttc tgttttaggt actggatgat aatcatggcc gtagtcttta gtccccttgc
     6961 gaggcttgtt cttttcctca tttaacttat tttctgcatc cttcgctttt ttctcctttt
     7021 gtttccggct ctccatcgcc ccggtaagtg cagccttatc tctggataag gattcattca
     7081 ttgcgtttaa agatgcctga gccgctttta atttctcatt ctgattctga atatccttat
     7141 caagatagcg aacctgatcc tgccacccgg agccatattc atgaggcggg ttataagtca
     7201 caaaatcatc tttatttgcc tctgcctctt ttaccttttt atcggcttcc tgaacagcag
     7261 cccttgcacc ggggagcgcc ttctctgtcg atgcaatgcg gctgtttaag gtcgcaatat
     7321 ttttatcttc ggcatccagc tcagctttcg ctttatcata gtctcttttc agcccctctt
     7381 ccggatgagc ggcgtcccat gcctgctggc gacgcttttc ttcctcctga cgctgtttta
     7441 cctgtgccgg ggtaagaaca tctgtcaccg acacataaac cggcttctgt ccggtctctt
     7501 tcgggaaacg aataacggcc tcacgggagt taccaccggc tgtaaaacca gccggacgtg
     7561 aatcaccttt ttcagcaaca atgccttttg gcggggcttt cgctgtcgga acacctttcg
     7621 gtacgctcac ctgcagagac gggagacccg gaatcgacac actgaatacc cccggacgtt
     7681 ttgtcggttt cgcatccacc acagggacac tcattggccg acctgcgaca accgcaatat
     7741 gctgccgctc atccttcacc acatccacaa cccgttgtct gacacgaacc gtcgcctggt
     7801 ccagtggtaa agtacttgcc ggagtctccg tcaccgcatc ggccggtaat gacgtcataa
     7861 tttttgacat cattttcgga tcatcttttg caatctcaga aggcagcaca ccgtagatcg
     7921 caatccccca cagaccaaac ttaaacggcc ccttcagggc agccagcaca tcagcaacgg
     7981 cggccgacaa cgcatcgccg gaaactgata aagccagtcc ttcagccccc ggagtagcca
     8041 gggcaggcag accgaaggcc atcgcagtgg ccgaagactg tccgccacca gagctaccgc
     8101 tatgattgcc gctgttacca ccattaccac cgggtttact cccgccgtta ccaaactccc
     8161 ccggattata gttatgaata tctccattcg gcgtatgcgt cgtaccccag cccggcccgc
     8221 tgtttggatc cgtaccacca cctgatgaag ggccaccttt accagacgta ccgttcacat
     8281 tcccactggc ctgaccacca ttatgtccca gacctgaatt acccggacct cgaccgtctc
     8341 caccactcat accgttttcc tctttgacat ttaaaacaat aaattaaagc caacatgtcg
     8401 cggtagggat acccgttacc ggataccccc cgcacagatc ccggcgtgcg cgatttacgc
     8461 accgggctcc tgcctcgggt gtctggcggt gaaccgctcc acaggccatg gatgaagaac
     8521 ccgaaccctt ggtagccatg cggctgccag tttgtttgct ttcgtccagg tcgtatcatc
     8581 cttctggctc ctgcgcctga gcgcccggcg ccagaggttt gttacgtgtg tcctgaactt
     8641 ctgcatggtg gggaagttgc ccggtaccga gtgatagttc aggtatccct gaaccactct
     8701 cctgagccat tttccctgtt cggggattga gtaatgccag cgccttcgca gaccgtcttt
     8761 gatggctttc agagttgccg tcatccgatc ccggcgggtc tttcgtatca gcatgaacct
     8821 gccgttgcga tctttcccgc tgatgtgcgt gaacccgagg aagttgaacg tttctggttt
     8881 gccttttccc ctgatggcac ggttttcggc agcgaagcgg ccgaactcca tcagacgggt
     8941 tttctccggg tgaaccgtga gtccgaactc cctcagtctg cgctgcatgg ctatacggaa
     9001 gcgccgggca tcgtatcgtt tgtcgaaccc gatgacgatg tcatcggcgt atctgaccat
     9061 taccacattg cctgtggcat agcgacgtcg ccactgatgc gcccacagat cgaagacgta
     9121 gtggaggtat atgtttgcca gcagcggtga gatgaccgca ccctgtgggg tgccttcctc
     9181 cgttgctcgc cattgaccct cctccgacgt cccggctgtg agccacttac gtatgagcct
     9241 gattaccctc cggtcgccga tccgatgctc tgtgaacctg atcagccatt cgtggctcac
     9301 cctgtcgaag aactgactga tgtcggcatc cagtacccag tttacgttag tgcgtaccag
     9361 ccctgtggcc agtgcgtcca gtgcatcgtg ctggcttcgc ccgggtctga acccgtatga
     9421 gaaccccata aagtcgtttt catagactgc gttcaggatt ttcaccagcg catactggac
     9481 gatcttgtcc tccagcgagg cgatgccgag cgggcgttgt tttccatccg cttttgggat
     9541 gtagtgacgc ctgccgggct gcgccctgta gctgccctga tgtagcctcc ggtgcagatc
     9601 tgttatgttg ttcttcatgt ttccggcgta gtccatccac ctgatgccat ccactccggc
     9661 ggccgctttc ctgctcaggg agaggaatgc ggcttccagt gcttcgactg tcagcaggtg
     9721 gaacaatgct gtaaaccgtt ctttcttccg ctgcttcgca gcttcccgca cgcgtgacag
     9781 cctctgtgac atgctttccc ggctctgtgt ccggcgcatg tgtggctgtt ccgcgttccc
     9841 cttggccccg ctccttcgct ccactgactc cgctcctttc gggttgttcg cctgcttcgc
     9901 cgctactatg agcgagtccg acttctcctc tccgtacatc accggctatg actcctcgtc
     9961 ttcccggtgc gggccatctc cgacactggc agatggtcag aggggagatc tcccggttcc
    10021 cgcgtagaga tcgtattgac atgccagggt ctcagacccc gccgggtcca tgtggcactc
    10081 gcagtatcgc accctatgat gttgccttcc gttaacagta caacgtcggc acccggtaat
    10141 ttaatataca tttcgtggct caatggctgg cctgtcaaca cccctgtcaa cgcttcgccc
    10201 catacctcgc ggtatgcaac gcatgactcg gggaccttgt ggattgctgg tccttcaatg
    10261 gtcggggact ttcacccctt gatctctaac cggtctcccg gcgcacactg tacatataca
    10321 cagtataatt attaccttac tcctcccgtt ttcaggtgtc aatatatgag attagttctc
    10381 atcttcaggc acaaaaaagc gcccggagcc acctgtaagc agatcgcgtc ttttcccgaa
    10441 aatttctcat ggcaacaaat tttattattt aagacattca tcacacttac atttcagatg
    10501 gtatagacac atctgaaagc taactggcat gaggagagga cctgcggtta aactgttcgg
    10561 gaaacctgag acgcccggag gccactatga cgcacctgaa actgactccc gctgaagaaa
    10621 agcgcctggc ggacgccttt gtcgctcagg cactggccat gggcctgaaa gggaacaaaa
    10681 cgctgccaaa ggcggtaaaa caacaggtgc gggaaagggt gcttcgtgcc attcaggacg
    10741 aacgtgaagc caggcgggcg caggaaatgc gtcgggagaa atacgaggct acggaaacca
    10801 ccttcacctg gcagccacag cgccgccggt aaggggtatt taccataacg tcgcattgcc
    10861 cgcaccgctc agacggtctg agggcgtttc cggcggctac gggataaaac aggcaacaaa
    10921 ccacccggaa aaccgccgga aacgcgcccg gtaaattgta acccgctgca tgcctatgca
    10981 cgcaactgca tggcgggccg tttggtgtaa aaacgatacg ataagccgtg tttatgtaaa
    11041 tgaacgtggc cgtaaagagt gacaaatata catgtaacat tttttactga caggtcatca
    11101 acccgatgcg tttaataccc tttatcctgc tggcattgtg cagcccctta ctcctgacgg
    11161 cctgtaagca ttccaccacc cagccgccga aaatttatac tctggataag tcatctaaaa
    11221 tcagtagcta cgctgcggcc atcaagaccg ctgttgaaat gcagatgttt gaaccagagg
    11281 cctatgcggg aaaaatgtgc accgtgcgga tatccataca acgtgatggc actctgaaca
    11341 gcgccattgc tgaaggagga gatccgaaac tctgtaaagc agcaatatca gccgttaccc
    11401 acgccaaaat tccgccggca ccggatgaaa atacctggca aatattcaaa aacgcccctc
    11461 tggatttcag accatgaaag cttttactct gacagtgctg gcggccatgt tcctcactcc
    11521 ggtacatgcg ataccactaa aacccgtaac cacactgcag ccaacctacc cggtacaggc
    11581 cgcagcaaaa cgcatcgaag gtcaggtgac cgccacgttt gatattctgg acgatggacg
    11641 agtggacaac gtgtcgctta ccggctgtca cctttttttt cgggaaacgc tgacggcaat
    11701 gaggcactgg ctatatcccg cagggcaccc ggcaatgcga caggttataa attttgaatt
    11761 cagtctgaaa aatgacggct tacaggatcc gcgaccagtc accacccaaa agctcaatga
    11821 tgatggttgc cgccagtaac aggtccttta acccgcaggc tgcaatagtc ctggatgatg
    11881 agtgagcctg tagcagccct gattagtgga agcgaagctg aaaaatgggc agcaaaagcc
    11941 gtatttctgg caaatagcg
//
