LOCUS       NZ_CP082009             5596 bp    DNA     circular CON 10-JAN-2024
DEFINITION  Klebsiella pneumoniae strain KP18-41 plasmid pKP18-41-3, complete
            sequence.
ACCESSION   NZ_CP082009
VERSION     NZ_CP082009.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN20928631
            Assembly: GCF_019823805.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 5596)
  AUTHORS   Qin,S. and Dong,H.
  TITLE     Klebsiella pneumoniae KP18-41
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5596)
  AUTHORS   Qin,S. and Dong,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2021) Institute of Drug Discovery & Development,
            Zhengzhou University, 100 Science Avenue, Zhengzhou City, Zhengzhou,
            Henan 450001, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: unicycler v. v.0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 12.0x
            Sequencing Technology             :: Illumina; Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/10/2024 03:31:36
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,694
            Genes (coding)                    :: 5,534
            CDSs (with protein)               :: 5,534
            Genes (RNA)                       :: 122
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 59 of 160
            Pseudo Genes (incomplete)         :: 108 of 160
            Pseudo Genes (internal stop)      :: 15 of 160
            Pseudo Genes (multiple problems)  :: 22 of 160
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP082009.1.
            Bacteria and source DNA available from Patient.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5596
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="KP18-41"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pKP18-41-3"
                     /geo_loc_name="China"
                     /lat_lon="34.73 N 113.70 E"
                     /collection_date="2018-06-21"
                     /collected_by="Zhengzhou University"
     gene            join(5387..5596,1..141)
                     /locus_tag="K6147_RS00005"
     CDS             join(5387..5596,1..141)
                     /locus_tag="K6147_RS00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004197756.1"
                     /translation="MYWLIAFLAFIFPGRSFADGYTAYYRCNGTQEASLTVKDNKLSMV
                     VGLSKFNRQLDTYRFKEKGIEVVMQRAASDDNSEAIMSFIMPDVNNMTLLVKNNGYIIV
                     KTTCWLTKTDKQ"
                     /name="hypothetical protein"
     gene            complement(211..477)
                     /locus_tag="K6147_RS00010"
     CDS             complement(211..477)
                     /locus_tag="K6147_RS00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262326.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_004197762.1"
                     /translation="MVWTINYSDRALKSLRKMDKQNARRIVDFMSLRIAVAADPRQSGK
                     PLKGELGEFWRYRVGDYRVLCEIRDDELVILAATIGHRREVYD"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(461..685)
                     /locus_tag="K6147_RS00015"
     CDS             complement(461..685)
                     /locus_tag="K6147_RS00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6290 family protein"
                     /protein_id="WP_000879771.1"
                     /translation="MLAIRLSDEIESRLDSLAKQTGRTKTFYAREAILAHLEDLEDYYL
                     SAETAARVRRGDEAVHSSEDVRKSLGLDD"
                     /name="DUF6290 family protein"
     gene            848..976
                     /locus_tag="K6147_RS29120"
     CDS             848..976
                     /locus_tag="K6147_RS29120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000523570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000523570.1"
                     /translation="MGLKPGPKRIADSTGEPDKRQRDNKKTPGNTDKLKPSKSSKK"
                     /name="hypothetical protein"
     gene            complement(1352..1564)
                     /locus_tag="K6147_RS00020"
     CDS             complement(1352..1564)
                     /locus_tag="K6147_RS00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_021527181.1"
                     /translation="MTELEKQLLSALEQLQQDYSKRLDEWESAFGEWRKMSGLMQRENA
                     ALSERVTRLSQQVERLSGQLQRLSR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(1571..2080)
                     /locus_tag="K6147_RS00025"
     CDS             complement(1571..2080)
                     /locus_tag="K6147_RS00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_032433972.1"
                     /translation="MNSLLTLAKDLEQKSKAQQQSTGEMLKAAFSEHEKSVRAELSESG
                     KRISAAIRAHEQGMSAAMQSNRLSVMRMVGRTWLTITMVSGLLFASLSGVLWYQGSLIA
                     SNLAEIDRQNAALSKLNAKTWGVTYLEDSNGRFLVLPKGTAVDRTQSWTVGNGRSKQNA
                     LRLVKE"
                     /name="MbeB family mobilization protein"
     gene            complement(<2115..2819)
                     /locus_tag="K6147_RS29195"
                     /pseudo=""
     CDS             complement(<2115..2819)
                     /locus_tag="K6147_RS29195"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(2758..3102)
                     /locus_tag="K6147_RS00035"
     CDS             complement(2758..3102)
                     /locus_tag="K6147_RS00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006122514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_062955149.1"
                     /translation="MAVKRTRFLGIRVTDGEYQQLLERCNGRQLAVWMRETCLDTRPAR
                     SLRLPSIDPVLLRQLAGMGNNLNQIARKINGGQWSGADRVQVVAALMAIDAELERLRHA
                     VLEKGADDDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            complement(3092..3268)
                     /locus_tag="K6147_RS00040"
     CDS             complement(3092..3268)
                     /locus_tag="K6147_RS00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004197757.1"
                     /translation="MQKPKKKPLVVKKRSWHPAVMANLMRQAQEEDYRREQMAELERSA
                     RSHFADIGGEDGG"
                     /name="hypothetical protein"
     gene            3908..4066
                     /locus_tag="K6147_RS29200"
     CDS             3908..4066
                     /locus_tag="K6147_RS29200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071596352.1"
                     /translation="MHEPPVQPDCCALSGNYHLESNPDGHAKTPLAAAIGNWIYTREIL
                     RGLEVGA"
                     /name="replication initiation protein"
     gene            4424..4750
                     /locus_tag="K6147_RS00045"
     CDS             4424..4750
                     /locus_tag="K6147_RS00045"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_050516389.1"
                     /translation="MRERTNIMLDIGLKQSLQRLSNKTKMSLSDLINQRLSGSLSVEER
                     IGEKPEWMRNLDPYLANHFSNIELPLPSPLKEYQEKMDEIDSIINDMMVLKEYFRAKFY
                     DEIK"
                     /name="hypothetical protein"
     gene            4776..5321
                     /locus_tag="K6147_RS00050"
     CDS             4776..5321
                     /locus_tag="K6147_RS00050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_023317533.1"
                     /translation="MFFKRYLTEKEIYKAALPIVRLQGRVNTSLRFLSKYSDVNKNGLI
                     ALHLNLLHFISYNIHGVESLKITQAGINKAVQQSPHADEIKNIAQAIGRKGPEYIEGLI
                     RNPWGKKNDNPLIFIMSIEESIKETLNSGYMLYRVAPITEGEAAAFMDVLKQLLLFEDN
                     FRLFGRDLVYKIEKLKIR"
                     /name="hypothetical protein"
     Typing          complement(3355..3431)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="91.25"
                     /coverage="98.0"
                     /evalue="1.04e-21"
     Typing          3620..4017
                     /name="rep_cluster_2370"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="89.526"
                     /coverage="91.0"
                     /evalue="3.03e-139"
     Typing          complement(1237..2768)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.804"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 agagctgctt ctgatgataa ctctgaggcc ataatgtctt ttattatgcc tgacgttaac
       61 aatatgacct tattagttaa aaataacggc tacattatcg ttaagacaac ctgctggtta
      121 actaaaaccg acaaacagta gaacacgtag ttagtcgagt atttaccata atacctgttg
      181 tcggcatcaa aaaagcccgc atagcgggct tcagtcgtaa acttcgcggc gatgtccaat
      241 cgtggcggca aggataacaa gctcgtcatc tcggatctca cacagaacgc gataatctcc
      301 cacccgatag cgccagaact cgcccagctc acctttgagc ggcttccctg actggcgagg
      361 atcggcagca actgcaatgc gtaaactcat aaaatccaca atccgtcgtg cgttctgttt
      421 gtccatcttg cgtaacgatt tgagcgcccg atcggaatag ttaatcgtcc agaccaagtg
      481 acttcctcac gtcttcagac gaatgcactg cttcatcacc acggcgaacg cgtgcagcag
      541 tttctgctga aagataataa tcctccaggt cttccagatg cgccagtatt gcttcccgcg
      601 cataaaacgt ctttgttctg ccggtttgct tcgccagcga gtccagacgg gactcaatct
      661 catcagataa ccggatagca agcatataaa cctcccgaac accatttgct atacatgtat
      721 atcaacagta ccacaatcaa cctaaaagaa caaaaaataa tcttgttagc ccctcttgat
      781 aatccgtaac gcggtcaata tatctagctt gagggctttc ctcttaacta ttgaaaggat
      841 aacgattatg ggtcttaagc cgggtccgaa acgaattgcc gattcaacag gggaacctga
      901 taaacgccag cgtgacaata aaaaaacgcc gggaaatact gacaaattga aacccagcaa
      961 atcttcaaaa aaataagtca gtagttaaaa gccctccggg gcttttttgt atctgccgtt
     1021 cacgctgatt aaacataaca cccgttgccc gcaccgcccc ggacgggttc gccgcgaacg
     1081 gggtggcagt caggtaaaac cggcaacaaa ccacccgata aaccgccccg ttcgcgacag
     1141 ataaatttta cctggctgca tagccatgca gccaggtgaa tgacatccgg tttcagcgta
     1201 aatgcccgat gagaaccctg ttttcctgca tttcagccca gcgacggacc gtcatagctt
     1261 cgctgccggg aacgcgcttc cgtcagttcc ttttcgtgct gccgttgctc gtgcgcccgt
     1321 tctgcggcca cggccatctc atgacgctgc actaccggct caaacgctgc agttgcccgc
     1381 tcaagcgttc cacctgctga ctcaagcgcg tcacgcgctc gctcagcgcc gcgttctccc
     1441 gttgcataag ccctgacatt ttccgccatt ccccgaaggc gctctcccac tcgtccagcc
     1501 ttttcgagta gtcctgctgt agctgctcta atgcgctcag caactgtttt tccagctcgg
     1561 tcatcggcac ttactccttc accagtctca gcgcgttctg cttcgaccgc ccgttcccga
     1621 ccgtccagct ctgcgtcctg tctacggccg tccccttcgg cagcaccaga aaacgtccgt
     1681 tgctgtcttc caggtacgtc acgccccagg tcttcgcgtt cagcttcgac agcgctgcgt
     1741 tctgccggtc gatttcggcc agatttgacg ctatcaagct cccctgatac cacaacaccc
     1801 cgctcaggct ggcgaacagc agtcctgaca ccatcgtgat ggtcagccat gtccgcccga
     1861 ccatacgcat cacgctcagg cggttcgact gcatggccgc gctcataccc tgctcgtggg
     1921 cgcggatggc ggcgctgatt ctcttcccgc tttcgctcag ttccgctctg acagacttct
     1981 cgtgctcgct gaatgcggct ttcagcattt cgccggtact ctgctgctgc gctttcgatt
     2041 tctgctctaa gtccttcgcc agcgttaaaa gactgttcat agatggctcc cttaagtcgg
     2101 atgtttcgcc ctccgtccgg gtcggcaatg ctgatgctgc tctttgtggt gcgcacgacc
     2161 tcaaaacctg cgctttccag cgcctcagtg acgtcctgac gcgttttaag ctcccctgac
     2221 gaggcgaggg ccagtaagcc ccgcgtaata gcctctgcgg cttcctgctt cgttttgggt
     2281 aacgaagatg gcgtcaccag cgcgcgccgg ttctccggtg cgttcgggtc gtgcagcccc
     2341 agtcgcccgt tcaccacggt ctgccacgca tcgatgcgcg gacggtctgc ccggtcgtag
     2401 tacggctgga gacgcctgcc ggtcagcagt tccgtgttcg gtaccagaaa attcagttcc
     2461 agccgcccct tgtcccggtg ttcaacccac agcacgctgt actggtcttt atcgagtccg
     2521 ggcatcagaa cccgctcgaa gctcgccatc agcttttcac gctggccggg cggtaaatcc
     2581 tgttcagcaa aagacaggac gccggaggtg tactttttgg cgtagggcga ggcgtcgata
     2641 agctcccgga cctcctccgg ctttccctgc aggacgatgg ccccgtcgcg ctggcggtct
     2701 ttccccagca gataatccac cggcccggca ccgccgcctc gccctcgcgg atgaaactta
     2761 acgatcatcg tctgccccct tttccagcac ggcatgccgc agccgctcga gttcggcatc
     2821 aatggccatc agcgcggcca ccacctgaac ccggtcagcg cctgaccact ggccgccgtt
     2881 aattttccgg gcgatctggt tgaggttgtt ccccatcccg gcaagctggc gcagcaaaac
     2941 cggatcgatg gacggcagcc gcaacgaccg cgccgggcgc gtatcgagac aggtttcccg
     3001 catccacacc gccagttgcc ttccattgca gcgctccagc agctgctggt actccccatc
     3061 ggtcacccgg atacctaaaa aacgggtacg cttaaccgcc atcctctcct ccgatatccg
     3121 caaaatgtga ccgggctgaa cgctcaagct ctgccatctg ctcccggcgg taatcttcct
     3181 cctgtgcctg acgcatcaga tttgccatca ctgccggatg ccaggatctt ttttttacga
     3241 cgagcggctt cttcttcggc ttctgcatta tccctccttc gcaaacgccg gacggcacgg
     3301 aaaagttttc cgggtcgctc cggcgttgga caagcgccag cgcgtcagtg gtgttagcgg
     3361 gtttcggggc gcagccctga accagtcaca gcgtttacgc tgtgtatact ggcttactat
     3421 gttggcatga gagcacgatt catgaaagtg cctcatgtgg caggtgtgat atgtggctca
     3481 ggagcgtccg caataccatg cacggacaga tgaaatgacg cctcctcgct cactcggtag
     3541 ctgcgctcct gccgttcggc tgcggcgggc gtgaccgact cacggataac gggataaagc
     3601 aaaggcgctc aaatcagaaa gggccggaga aaaggatgcg gcgttgccgt ttttccatag
     3661 gctccgcctc ccctgacgag catcacagaa atggacgctc aagtcagagg aggacgaaac
     3721 ccgacaggac ttaaagatgc cggtgatttc tctccggcgg ctccctcgtg cgctctcctg
     3781 ttccgaccct gccgtttacc ggatacctgt tccgcctttt tccctttcgg gaagcgtggc
     3841 gctttctcat agctcacgct gctggtatct cggctcggtt taggtcgttc gctccaagct
     3901 gggctgtatg cacgaacccc ccgttcagcc cgactgctgc gccctttccg gtaactatca
     3961 tcttgagtcc aacccggacg gacacgcaaa aacgccactg gcagcagcca ttggtaactg
     4021 gatatatacg agagagatat tgagaggtct tgaagtgggt gcctaactgc ggctacactg
     4081 aaagaacagt tttggtatct gcggtctcgt atacccagtt acccggttaa gcagttcccc
     4141 aactgactta accttcgatc aaaccgcctc cccaggcggt tttttcgttt acagtgcaag
     4201 agattacgcg cagaaaaaaa ggatctcaag aagatccttt aaatgatcgt cagttctgtt
     4261 atccccggag ctccatccat cccacggaaa aacacccgcc actgatgccc tcagcgcact
     4321 gcacatcatg tacaaaccac tccctccctc cttttttatt gcgtttctta atgcgcaatg
     4381 gtagctttgt gcgcaacaaa aaacgcaata gcaagggggg gtaatgagag aaagaacaaa
     4441 tatcatgcta gatataggat taaagcaatc cttacaacgg ctttcgaata aaaccaaaat
     4501 gagtctaagt gatcttatta accagagatt atcaggttca ctttcagttg aagaacgcat
     4561 tggcgaaaag cctgaatgga tgagaaacct tgatccctat ctcgccaatc acttttcaaa
     4621 tattgagtta ccgctccctt caccattaaa agaataccaa gaaaaaatgg atgagataga
     4681 ttcaattatt aatgacatga tggttctaaa ggaatatttc agagctaagt tttacgacga
     4741 aataaaatag gcacgctata cggaggcgga gaattatgtt ttttaaacgg tatttaacgg
     4801 agaaggaaat atacaaggca gcactcccaa ttgttaggct tcaagggaga gttaacactt
     4861 cattacgttt tttatccaaa tactcagatg taaacaaaaa tggattaatt gcattgcatc
     4921 ttaatttatt acattttatc tcatacaaca tacacggtgt agagtctctg aaaatcactc
     4981 aagcaggcat aaacaaagct gtacaacaaa gccctcacgc agatgaaata aaaaatattg
     5041 cgcaggcaat aggacgcaaa ggacctgagt acatcgaagg attaattaga aacccatggg
     5101 ggaagaaaaa tgataatcca ctaatattta taatgagcat tgaagaaagt ataaaagaaa
     5161 cccttaactc aggttatatg ttatatagag tagctcccat aactgagggt gaagcagctg
     5221 ctttcatgga tgttcttaaa caattattat tatttgagga taattttcgc ctttttggta
     5281 gagacttggt ttataaaata gaaaaattaa aaatcagata acaaagtatg gtgaacgaaa
     5341 aatgaaagag aggatagtga aatgtgtaat accaagattc tcaactatgt attggttaat
     5401 tgcgttccta gcatttatat tccccggtcg atcttttgct gacggttaca ccgcttacta
     5461 ccgatgtaat ggtactcagg aagcatctct tactgttaaa gataacaaac taagtatggt
     5521 tgtaggtctt tctaagttta ataggcaact ggatacttat agattcaaag aaaagggaat
     5581 cgaagtagta atgcag
//
