LOCUS       NZ_CP082008            10060 bp    DNA     circular CON 10-JAN-2024
DEFINITION  Klebsiella pneumoniae strain KP18-8 plasmid pKP18-8-4, complete
            sequence.
ACCESSION   NZ_CP082008
VERSION     NZ_CP082008.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN20928630
            Assembly: GCF_019823785.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 10060)
  AUTHORS   Qin,S. and Dong,H.
  TITLE     Klebsiella pneumoniae KP18-8
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10060)
  AUTHORS   Qin,S. and Dong,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2021) Institute of Drug Discovery & Development,
            Zhengzhou University, 100 Science Avenue, Zhengzhou City, Zhengzhou,
            Henan 450001, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: unicycler v. v.0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 12.0x
            Sequencing Technology             :: Illumina; Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/10/2024 03:26:22
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,567
            Genes (coding)                    :: 5,391
            CDSs (with protein)               :: 5,391
            Genes (RNA)                       :: 126
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 176
            CDSs (without protein)            :: 176
            Pseudo Genes (ambiguous residues) :: 0 of 176
            Pseudo Genes (frameshifted)       :: 67 of 176
            Pseudo Genes (incomplete)         :: 113 of 176
            Pseudo Genes (internal stop)      :: 25 of 176
            Pseudo Genes (multiple problems)  :: 26 of 176
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP082008.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..10060
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="KP18-8"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pKP18-8-4"
                     /geo_loc_name="China"
                     /lat_lon="34.73 N 113.70 E"
                     /collection_date="2018-06-11"
                     /collected_by="Zhengzhou University"
     gene            complement(join(8609..10060,1..234))
                     /locus_tag="K6148_RS28320"
     CDS             complement(join(8609..10060,1..234))
                     /locus_tag="K6148_RS28320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase domain-containing
                     protein"
                     /protein_id="WP_032440457.1"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSVSGDALSAAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPKMMSK
                     IMTSLPADAVTETPASTLPLDQATVRVRQRVVDVVKDERQHIAVVAGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPSLQVSVPKGVPTAKAPPKGIVAEKGDSRPAGFTAGGNSREAVIR
                     FPKETGQKPVYVSVTDVLTPAQVKQRQEEEKRRQQAWDAAHPEEGLKRDYDKAKAELDA
                     EDKNIATLNSRIASTEKALPGARAAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNEKLKAAQASLNAMNESLSRDKAALTGAMESRKQKEKKAKDAENKLNE
                     EKNKPRKGTKDYGHDYHPVPKTEDIKGLGELKRGDPKTPKQGGGGKRARWYGDKKRKIY
                     EWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPKRNIKKYL"
                     /name="colicin-like bacteriocin tRNase domain-containing
                     protein"
     gene            561..806
                     /locus_tag="K6148_RS28325"
     CDS             561..806
                     /locus_tag="K6148_RS28325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032440458.1"
                     /translation="MTHLKLTPAEEKRLADAFVAQALAMGLKGNKTLPKAVKQQVRERV
                     LRAIQDEREARRAQEMRREKYEATETTFTWQPQRRR"
                     /name="hypothetical protein"
     gene            1080..1451
                     /gene="tolA"
                     /locus_tag="K6148_RS28330"
     CDS             1080..1451
                     /gene="tolA"
                     /locus_tag="K6148_RS28330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237044.1"
                     /GO_component="GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA]"
                     /GO_function="GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="WP_001237044.1"
                     /translation="MRLIPFILLALCSPLLLTACKHSTTQPPKIYTLDKSSKISSYAAA
                     IKTAVEMQMFEPEAYAGKMCTVRISIQRDGTLNSAIAEGGDPKLCKAAISAVTHAKIPP
                     APDENTWQIFKNAPLDFRP"
                     /name="tolA"
     gene            1448..1813
                     /locus_tag="K6148_RS28570"
     CDS             1448..1813
                     /locus_tag="K6148_RS28570"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /GO_function="GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="WP_072354022.1"
                     /translation="MKAFTLTVLAAMFLTPVHAIPLKPVTTLQPTYPVQAAAKRIEGQV
                     TATFDILDDGRVDNVSLTGCHLFFRETLTAMRHWLYPAGHPAMRQVINFEFSLKNDGLQ
                     DPRPVTTQKLNDDGCRQ"
                     /name="TonB family protein"
     gene            1988..2266
                     /locus_tag="K6148_RS28340"
     CDS             1988..2266
                     /locus_tag="K6148_RS28340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014542884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family ribbon-helix-helix protein"
                     /protein_id="WP_000093040.1"
                     /translation="MSSATSIRLDEELKDRLKTLADDRHRSAHALMLEAITEYIDREEK
                     RSQYLRDGQAAWQHYQETGLHLTAEEAEAWISTWGTENEQDAPPCHR"
                     /name="CopG family ribbon-helix-helix protein"
     gene            2254..2568
                     /locus_tag="K6148_RS28345"
     CDS             2254..2568
                     /locus_tag="K6148_RS28345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001136729.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]; GO:0009432 - SOS response [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_071177730.1"
                     /translation="MPQVTISALAQRDLQRLQDFLKTKNRLAARKGGEVIVRAIQQLKT
                     LPDIGRPVPFLPLEYKELVIGFGDSGYVMLYRHDREMDQIVIVTVRHQKESGYPGADSL
                     "
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            2732..3160
                     /locus_tag="K6148_RS28350"
     CDS             2732..3160
                     /locus_tag="K6148_RS28350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001140599.1"
                     /translation="MAKTLLNLSQAAQAAGITRRTLYNHVKQGKVTVSRDGKNNPVVDV
                     SELIRVYGNVNIPEKQIPGISHRENTQKNFPQEQLLAMQKELADLRQAVTLMLEDKTSR
                     EEERRQHDDERRKLQAEVDRLTTELTQKKKRFWSGWFS"
                     /name="hypothetical protein"
     gene            3186..3365
                     /locus_tag="K6148_RS28355"
     CDS             3186..3365
                     /locus_tag="K6148_RS28355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165970.1"
                     /translation="MTKQEKTALNMAKFIKDQSLLLLEKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(3392..3922)
                     /locus_tag="K6148_RS28360"
     CDS             complement(3392..3922)
                     /locus_tag="K6148_RS28360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_071177729.1"
                     /translation="MALYMVFIEDVEDKDGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGENPQWERDKPTGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQINAMIEVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(3929..4660)
                     /gene="mobC"
                     /locus_tag="K6148_RS28365"
     CDS             complement(3929..4660)
                     /gene="mobC"
                     /locus_tag="K6148_RS28365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WP_071177728.1"
                     /translation="MTLEQYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRAKYPVDGRQVSVWGLTPHGVAFSFDEDEPLTDVIPFQP
                     SRVSAAQLPHRLAVQSLRLSMEGRGATGWRYLHRMALKGMKVPDALAELDGRTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRRANGIDEIWYICPDRPTLVRVQRAILAVDEIVNPQTGEA
                     RKTVELDRERLFACFKFMTTE"
                     /name="mobC"
     gene            complement(4660..6624)
                     /locus_tag="K6148_RS28370"
     CDS             complement(4660..6624)
                     /locus_tag="K6148_RS28370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_222698189.1"
                     /translation="MFNTDSLAWQWHIPELARQIYPWVSWLYREYYSLAGFFLVIGLFF
                     GLAELWAGRMRFVGLSHKARREYADRYKRDNNRTVPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLV
                     AGVLLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSTEQARKQQAVFLGKDEQGQPVLVPRDTWRKTNIQILGLPGSGKSAMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLV
                     AGFNLSETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     KLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFRRWPHASIMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPADFV
                     KTTVLDNTPNRWFYRAASQESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARLAFSSPLRVERREISLKTFPVLAKTDPLAEY
                     LPPADGGGPKADDDFAGLY"
                     /name="TraM recognition domain-containing protein"
     gene            complement(8108..8257)
                     /locus_tag="K6148_RS28375"
     CDS             complement(8108..8257)
                     /locus_tag="K6148_RS28375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_032440454.1"
                     /translation="MKKVKTIFLFILIVAGFLLAACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     gene            complement(8342..8599)
                     /locus_tag="K6148_RS28380"
     CDS             complement(8342..8599)
                     /locus_tag="K6148_RS28380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /GO_function="GO:0015643 - toxic substance binding
                     [Evidence IEA]"
                     /GO_process="GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E3-like toxin immunity protein"
                     /protein_id="WP_032440455.1"
                     /translation="MGLKLRLTWFDKKTEEFKGEEYSKDFGDDGSVIESLGMPLKDNIN
                     NGEFDLKKEWVPLLQPFFKNQIKPDVDNCYIAFDYRDGNW"
                     /name="colicin E3-like toxin immunity protein"
     BGC             3609..10060
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          7427..8005
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.282"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(3933..4660)
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.416"
                     /coverage="100.0"
                     /evalue="1.34e-140"
ORIGIN
        1 accaccatta ccaccgggtt tactcccgcc gttaccaaac tcccccggat tatagttatg
       61 aatatctcca ttcggcgtat gcgtcgtacc ccagcccggc ccgctgtttg gatccgtacc
      121 accacctgat gaagggccac ctttaccaga cgtaccgttc acattcccac tggcctgacc
      181 accattatgt cccagacctg aattacccgg acctcgaccg tctccaccac tcataccgtt
      241 ttcctctttg acatttaaaa caataaatta aagccaacat actgtacata tacacagtat
      301 aattattacc ttactcctcc cgttttcagg tgtcaatata tgagattagt tctcatcttc
      361 aggcacaaaa aagcgcccgg agccacctgt aagcagatcg cgtcttttcc cgaaaatttc
      421 tcatggcaac aaattttatt atttaagaca ttcatcacac ttacatttca gatggtatag
      481 acacatctga aagctaactg gcatgaggag aggacctgcg gttaaactgt tcgggaaacc
      541 tgagacgccc ggaggccact atgacgcacc tgaaactgac tcccgctgaa gaaaagcgcc
      601 tggcggacgc ctttgtcgct caggcactgg ccatgggcct gaaagggaac aaaacgctgc
      661 caaaggcggt aaaacaacag gtgcgggaaa gggtgcttcg tgccattcag gacgaacgtg
      721 aagccaggcg ggcgcaggaa atgcgtcggg agaaatacga ggctacggaa accaccttca
      781 cctggcagcc acagcgccgc cggtaagggg tatttaccat aacgtcgcat tgcccgcacc
      841 gctcagacgg tctgagggcg tttccggcgg ctacgggata aaacaggcaa caaaccaccc
      901 ggaaaaccgc cggaaacgcg cccggtaaat tgtaacccgc tgcatgccta tgcacgcaac
      961 tgcatggcgg gccgtttggt gtaaaaacga tacgataagc cgtgtttatg taaatgaacg
     1021 tggccgtaaa gagtgacaaa tatacatgta acatttttta ctgacaggtc atcaacccga
     1081 tgcgtttaat accctttatc ctgctggcat tgtgcagccc cttactcctg acggcctgta
     1141 agcattccac cacccagccg ccgaaaattt atactctgga taagtcatct aaaatcagta
     1201 gctacgctgc ggccatcaag accgctgttg aaatgcagat gtttgaacca gaggcctatg
     1261 cgggaaaaat gtgcaccgtg cggatatcca tacaacgtga tggcactctg aacagcgcca
     1321 ttgctgaagg aggagatccg aaactctgta aagcagcaat atcagccgtt acccacgcca
     1381 aaattccgcc ggcaccggat gaaaatacct ggcaaatatt caaaaacgcc cctctggatt
     1441 tcagaccatg aaagctttta ctctgacagt gctggcggcc atgttcctca ctccggtaca
     1501 tgcgatacca ctaaaacccg taaccacact gcagccaacc tacccggtac aggccgcagc
     1561 aaaacgcatc gaaggtcagg tgaccgccac gtttgatatt ctggacgatg gacgagtgga
     1621 caacgtgtcg cttaccggct gtcacctttt ttttcgggaa acgctgacgg caatgaggca
     1681 ctggctatat cccgcagggc acccggcaat gcgacaggtt ataaattttg aattcagtct
     1741 gaaaaatgac ggcttacagg atccgcgacc agtcaccacc caaaagctca atgatgatgg
     1801 ttgccgccag taacaggtcc tttaacccgc aggctgcaat agtcctggat gatgagtgag
     1861 cctgtagcag ccctgattag tggaagcgaa gctgaaaaat gggcagcaaa agccgtattt
     1921 ctggcaaata gcgccgccac ggattaaagt agtcatgtgt aatacatatg actacaaagg
     1981 ggcttttatg tcatcagcaa catccatcag gctcgatgaa gaacttaagg accggctgaa
     2041 aacgctggcc gatgaccggc accgctcagc gcacgcgcta atgctggagg caatcaccga
     2101 atacatcgac cgggaagaga agcgcagcca gtacctgcgg gacggacagg ccgcatggca
     2161 gcactatcag gaaacgggtc tgcaccttac tgcggaagaa gcggaagcct ggataagtac
     2221 ctggggaact gagaacgagc aggatgctcc gccatgccac aggtaacgat ttccgcgctg
     2281 gcacagcgcg atttacaacg ccttcaggac tttctcaaaa ccaaaaatcg gctggcagcc
     2341 agaaaaggag gtgaggtgat cgtccgggct atccagcaac tgaaaacatt gccagacatt
     2401 ggccgcccgg tgccgtttct gccgctggaa tataaggaac tggtgatcgg gtttggcgac
     2461 agtggctatg tgatgctcta ccgccacgac cgggaaatgg accagattgt gatcgtcacc
     2521 gtcaggcatc agaaagaatc cgggtatccg ggggcagaca gcctctaata accgatgacg
     2581 ggggcgcaat tcattgcgtg gccgtcgccg gtttgacaag cgcagcgcgt cagaaaacca
     2641 catcctgaac ggaaaaacgg gtaaaattca gcgatacatt taccgtgaga aattacacac
     2701 ctttcacacc cgataaaatc aggggtatac agtggcgaaa acattgctca acctttcaca
     2761 ggcagcacaa gccgcgggca ttaccagaag aacgctatac aatcatgtaa aacagggaaa
     2821 ggttaccgtg tcacgtgacg ggaaaaacaa ccctgtcgtg gatgtgtcag aactgatccg
     2881 tgtatacgga aatgtgaata tccctgagaa acaaattccc ggcatttcac accgtgaaaa
     2941 tacacaaaaa aatttcccac aggaacagct tctggcaatg caaaaggaac tggccgatct
     3001 ccggcaggct gtcaccctga tgctggaaga taaaacatcc cgggaagaag aacgccgcca
     3061 gcacgatgat gagcgccgga aactccaggc cgaagtggac agattgacaa cagaactgac
     3121 acagaagaaa aaacgcttct ggtccggctg gtttagctga gaacttaccg aagggaatgg
     3181 agaacatgac gaagcaggaa aaaactgccc tcaatatggc taaattcatt aaggatcagt
     3241 cgctgctgtt gctggaaaag ctgaacgagc tggacatgga taccccggcc gacatgtgcg
     3301 aacggcttca cgaggatgcg gaaacactct ggcaggtgat gcgggataag ctcggggaag
     3361 aataacagca ccggccatgc cggtgctggc gttatttttt gccgaacggc caccaccggc
     3421 gggcgggttt gtcctccggc agttcaggca ccggcacgtt aaaatagcgg ctgttatatt
     3481 cccgcatcag ctccgctttc agcttctcgt tcaccgcgct ttccccgtgc cgctcgctga
     3541 ccacctcaat catcgcgtta atctgctccg gtgagcactt cccggcatca accacctgca
     3601 caccgttctc gatggcgaac cgctccacct ttcgatagag gtaatcacac agcttcattc
     3661 ctgtgggttt gtcccgttcc cattgcggat tttcaccggt gaaaaagtac gtggcttttt
     3721 cgtcgctgtt gagccgctcg tcatacatcc cgatggattt catcatcgcc agatgctggg
     3781 cccggtcgac aaaacagaaa acatacgccc cggtctgcca ggcgagcacc accatgtctt
     3841 ccagatgctc gctggttggc ataatgcagc gctcgccgtc tttgtcttcc acgtcctcaa
     3901 taaataccat gtacagcgcc ataatccctc actccgttgt cataaattta aagcacgcga
     3961 acagcctctc ccggtccagc tccaccgttt tgcgggcctc gccggtctgt ggattcacga
     4021 tttcatccac cgccagaatc gcccgctgta cccggacaag cgtcggccgg tcagggcaga
     4081 tgtaccagat ttcatcgatg ccgttcgccc ggcggttgaa cagatacccg gacaccactt
     4141 cctgataccg cctgcggctc ttgacggtgc gctctacctc aaatgccacc gttctgccgt
     4201 ccagctccgc cagcgcgtcc ggcactttca tccctttcag ggccatacgg tgcaggtacc
     4261 gccagccggt ggccccccgg ccctccatgg agaggcgcag ggactgcacg gccagccggt
     4321 gcggcagctg cgccgccgac acccttgacg gctgaaacgg aataacgtcc gtcagcggct
     4381 cgtcctcgtc gaacgagaac gccaccccgt gcggcgtcag cccccacacg ctgacctgac
     4441 ggccgtcaac cgggtatttc gcccggacca gcagcccttt cgccaccaga ctgtccagga
     4501 agcggtaaac cggctgtacc gccgcaatcc ccagccgtcg cgcaaccagc tccgccgtgg
     4561 aacacagctc ttcccgcagc cagcgcaggg tcagccgtgt tttctccgcg ttccgcgcct
     4621 gccgcttcgc gtggctgacg ttgtactgct ccagtgtcat cagtaaagcc ccgcaaaatc
     4681 atcatccgct tttggtccgc cgccgtcggc cggaggcagg tactccgcca gcgggtccgt
     4741 tttcgccagc accgggaagg ttttcagcga aatttcccgg cgctcaaccc gcagcgggct
     4801 gctgaacgcc agccgcgcca cgcccagccc ggtcatcacc gcgaacccgt caggcaggtg
     4861 ttgcagggtg ttcacgtcaa acagcggccg cgcctccttc tggatgaagc tgtcgccgct
     4921 gatgtgctcc acgttccccg cctcccggct ggcccggcgg cgctccacgt ccacccggat
     4981 ctccccggtc tggcccgccg cccactgcgc ggactcctgg ctggccgcgc gatagaacca
     5041 gcggttcggc gtgttatcca gtaccgtggt tttcacaaaa tcagcgggca gatcctgccc
     5101 gcactgaccg aagtcgccca gcgactggtg cgcgagcagc aggttgcagt cccggctgcg
     5161 cagcgtcccc agcgcgttca gcacgtactt cgacagcaga tatttaatct catcgagcat
     5221 gatgctcgcg tgcggccacc tccggaactc atcccgcgca atgatgattt gcgcgcagcg
     5281 ggcaaacagc atcttctgca cccggatcac cgcctcatcg tccatcgagc cgatgacgta
     5341 caggcagccg ccgccgttga ttatccccgc cacatcgata ccgctgtccg tctgcaggac
     5401 gctcaggtcg gccacgttct ccagcttggt gatcagcccc ttcacgtcct ttttcagggc
     5461 ctccggcaac gcgtacgccg ccgccagcac ctgctgaatg tttgccccct gcgggaactg
     5521 ctccgcaatc agcttcgccg ctttctgctc gctgatgcgg tagtggtccg ccacgtcgcc
     5581 ggtctcactc aggttaaacc cggcaaccag cagattcttc agggcgtact ggtcgagatc
     5641 gcggaacaga ttcagctgcg ccggtttccc cggccgcaga tccagcaggg tgaagtccgg
     5701 gcagtgcgcc cggaagacgt gcggcgccca cgcgtcccct ttggggtcga agtacaccac
     5761 agcgtccccg aaatcgcgta cgcagcgaat gagcgcgttg gtggccatcg cgctcttgcc
     5821 gctcccgggc aggccgagga tctgaatatt ggtcttgcgc caggtatcac gagggaccag
     5881 caccggctgg ccctgctcat ccttacccag aaacaccgcc tgctgtttcc gggcctgctc
     5941 cgtggaaaaa tcggccagcg cgttaaccgg taccggctcc ccgaaatgca ggtggcggac
     6001 gtctgtcatt tctccggtcc gctggctgct gcgccggctc tcctgattca gtttcgcgtt
     6061 caccgcctcg tcagcggccg caaaatgacc atcaagcagc aggaacgcca gcagccgcgc
     6121 cagaaggacg ccggcgacaa gccctccggc acaccaggct atcccgaaca ggttagccgg
     6181 ctgcagcagg taataatctg cgttcgcata gaggtccggg cgcaggacgg tttctcccca
     6241 gctctcgata aacacggcga caaccagccc ccccagaagg gccagaccgg cagcgccggt
     6301 cagccagtac gcgtagttcc agacatgtcg ccgtgccctg accatccgcc cgggtgaatc
     6361 cagcgaggcc agcaccgaaa atggcacagt ccggttatta tcgcgcttat agcgatcggc
     6421 atactcccgc cgcgccttat ggctcagccc cacaaaacgc attcggccgg cccagagttc
     6481 agccagaccg aaaaacaggc cgataaccag aaaaaaaccg gccagactgt aatactcccg
     6541 gtacagccag ctgacccacg ggtaaatctg ccgggccagc tccgggatat gccactgcca
     6601 ggcaagcgaa tccgtattaa acatgcaatc tctctccttc agacgacggg tttaccggcg
     6661 tgatgcgcag cccccgctgc acaagccgcg gacatgcccc aaggcatggc ggcggcggtc
     6721 acgtccgtgg tgattgcgtt cagctttcac cgcggaggcc agcctctttc cgccccatgc
     6781 ggaaaaggcg cagccccgga ggaacaacga gcacgcccca gcgtgtgaga ggaaaaggcg
     6841 gcaccccagt gcggtctttt ccggtgttcc ggcccccgtc agagttcgcc atagcgagcg
     6901 gctgaccacc cgcaaaatca ggagcagctt tgctgcttcg tcaggtgcgt ccagcaccgt
     6961 ggatttttgt gggtggtcag gcgtgagcct gacgggggcc gggtgaattt ctggcgtggc
     7021 cggcgtccag ccggacagag ccagaaagag gggaaaagac cgtgcgtcgg tggcgtccag
     7081 ccagcgtaag caaggtcttt tcccctgaag tcagccacgc cccagcgggg atgacttgca
     7141 tttctccgca aggagaaaaa agcaacagag ataggttaac gtaaaatgtt cagcgaaaaa
     7201 ccgacatggt tcacctatcc tgataattga tcgtcaggga atagaaagac ttaatcaggg
     7261 gacaatatcc cacatcagcg cgtccggtga ccgtcttgca ggtgccgggg aaattgcgcc
     7321 tcctcgctca ctcggtcgct acgctccggg cgtgagactg cggcgggcgc tgcggacaca
     7381 tacaaagtta cccacagatt ccgtggataa gcaggggact aacatgtgag gcaaaacggc
     7441 agggccgcgc cggtggcgtt tttccatagg ctccgccctc ctgccagagt tcacataaac
     7501 agacgctttt tcggtgcatc tgtgggagcc gtgaggctca accatgaatc tgacagtacg
     7561 ggcgaaaccc gacaggactt aaagatcccc accgtttccg gtaggtcgct ccctcttgcg
     7621 ctctcctgtt ccgaccctgc cgtttaccgg atacctgtcc gccgttctcc cttgcgggaa
     7681 gtgtggcgct ttctcatagc tcacacactg gtatctcggc tcggtgtagg tcgttcgctc
     7741 caagctgggc tgtaagcaag aactccccgt tcagcccgac tgctgcgcct tatccggtaa
     7801 ctgttcactt gagtccaacc cgggaaagca cgcaaaacgc cactggcagc agccattggt
     7861 aactgggagt tcgcagagga tttgtttagc taaacacgcg gttgctcttg aagtgtgcgc
     7921 caaagtccgg ctacactgga aggacagatt tggttgctgt gctctgcgaa agccagttac
     7981 cacggttaag cagttcccca actgacttaa ccttcgatca aaccacctcc ccaggtggtt
     8041 ttttcgttta cagggcaaaa gattacgcgc agaaaaaaag gatctcaaga agatcctttg
     8101 atcttttcta ctgaaccgcg atccccgtca gttcagagga ggacgatggt gccaccgttc
     8161 cgccctgaac atcccggata taatttgcct gacatgcggc cagaagaaag ccagcaacaa
     8221 tcaatataaa aagaaaaata gtctttacct ttttcatata ctttccctgt atttaaagac
     8281 tccggcattc atccctgaac accggaagtc agtatttcaa atcatccttg ataatttttt
     8341 gttaccagtt accgtcccga taatcaaaag ctatataaca attatccaca tcaggtttta
     8401 tttgattttt aaaaaatggc tgtagtaaag gaacccattc tttcttcaaa tcaaactcac
     8461 cattattaat attatccttt aaaggcattc ccaagctttc aatgacagaa ccatcgtcac
     8521 caaagtcttt agaatactct tcccctttaa actcttcagt tttcttatca aaccatgtca
     8581 aacgtaattt aagccccata tttacctctc aaagatattt tttaatgttc cgttttggat
     8641 ctggcccttt aacctgctta cccgttttgg gatcaaatgc gcctaggtgt tcgccatcac
     8701 tggcacgata cccttcaagc tcaccgtgct gggaatccca ttcataaatt ttacgttttt
     8761 tatcgccata ccatcgggcc cgcttaccac caccaccttg tttaggtgtt ttaggatcac
     8821 ctcgctttaa ctctccaagt ccctttatgt cttctgtttt aggtactgga tgataatcat
     8881 ggccgtagtc tttagtcccc ttgcgaggct tgttcttttc ctcatttaac ttattttctg
     8941 catccttcgc ttttttctcc ttttgtttcc ggctctccat cgccccggta agtgcagcct
     9001 tatctctgga taaggattca ttcattgcgt ttaaagatgc ctgagccgct tttaatttct
     9061 cattctgatt ctgaatatcc ttatcaagat agcgaacctg atcctgccac ccggagccat
     9121 attcatgagg cgggttataa gtcacaaaat catctttatt tgcctctgcc tcttttacct
     9181 ttttatcggc ttcctgaaca gcagcccttg caccggggag cgccttctct gtcgatgcaa
     9241 tgcggctgtt taaggtcgca atatttttat cttcggcatc cagctcagct ttcgctttat
     9301 catagtctct tttcagcccc tcttccggat gagcggcgtc ccatgcctgc tggcgacgct
     9361 tttcttcctc ctgacgctgt tttacctgtg ccggggtaag aacatctgtc accgacacat
     9421 aaaccggctt ctgtccggtc tctttcggga aacgaataac ggcctcacgg gagttaccac
     9481 cggctgtaaa accagccgga cgtgaatcac ctttttcagc aacaatgcct tttggcgggg
     9541 ctttcgctgt cggaacacct ttcggtacgc tcacctgcag agacgggaga cccggaatcg
     9601 acacactgaa tacccccgga cgttttgtcg gtttcgcatc caccacaggg acactcattg
     9661 gccgacctgc gacaaccgca atatgctgcc gctcatcctt caccacatcc acaacccgtt
     9721 gtctgacacg aaccgtcgcc tggtccagtg gtaaagtact tgccggagtc tccgtcaccg
     9781 catcggccgg taatgacgtc ataatttttg acatcatttt cggatcatct tttgcaatct
     9841 cagaaggcag cacaccgtag atcgcaatcc cccacagacc aaacttaaac ggccccttca
     9901 gggcagccag cacatcagca acggcggccg acaacgcatc gccggaaact gataaagcca
     9961 gtccttcagc ccccggagta gccagggcag gcagaccgaa ggccatcgca gtggccgaag
    10021 actgtccgcc accagagcta ccgctatgat tgccgctgtt
//
