LOCUS       NZ_CP082002            10049 bp    DNA     circular CON 10-JAN-2024
DEFINITION  Klebsiella pneumoniae strain KP18-1 plasmid pKP18-1-2, complete
            sequence.
ACCESSION   NZ_CP082002
VERSION     NZ_CP082002.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN20928629
            Assembly: GCF_019823765.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 10049)
  AUTHORS   Qin,S. and Dong,H.
  TITLE     Klebsiella pneumoniae KP19-2029
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10049)
  AUTHORS   Qin,S. and Dong,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2021) Institute of Drug Discovery & Development,
            Zhengzhou University, 100 Science Avenue, Zhengzhou City, Zhengzhou,
            Henan 450001, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: unicycler v. v.0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 12.0x
            Sequencing Technology             :: Illumina; Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/10/2024 03:32:37
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,822
            CDSs (total)                      :: 5,700
            Genes (coding)                    :: 5,539
            CDSs (with protein)               :: 5,539
            Genes (RNA)                       :: 122
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 161
            CDSs (without protein)            :: 161
            Pseudo Genes (ambiguous residues) :: 0 of 161
            Pseudo Genes (frameshifted)       :: 59 of 161
            Pseudo Genes (incomplete)         :: 109 of 161
            Pseudo Genes (internal stop)      :: 15 of 161
            Pseudo Genes (multiple problems)  :: 22 of 161
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP082002.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..10049
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="KP18-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pKP18-1-2"
                     /geo_loc_name="China"
                     /lat_lon="34.73 N 113.70 E"
                     /collection_date="2018-06-07"
                     /collected_by="Zhengzhou University"
     gene            complement(10..168)
                     /locus_tag="K6149_RS29355"
     CDS             complement(10..168)
                     /locus_tag="K6149_RS29355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071596352.1"
                     /translation="MHEPPVQPDCCALSGNYHLESNPDGHAKTPLAAAIGNWIYTREIL
                     RGLEVGA"
                     /name="replication initiation protein"
     gene            1300..3264
                     /locus_tag="K6149_RS27840"
     CDS             1300..3264
                     /locus_tag="K6149_RS27840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_071177727.1"
                     /translation="MFNTDSLAWQWHIPELARQIYPWVSWLYREYYSLAGFFLVIGLFF
                     GLAELWAGRMRFVGLSHKARREYADRYKRDNNRTVPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLV
                     AGVLLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSTEQARKQQAVFLGKDEQGQPVLVPRDTWRKTNIQILGLPGSGKSAMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLV
                     AGFNLSETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     KLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFRRWPHASIMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPADFV
                     KTTVLDNTPIRWFYRAASQESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARLAFSSPLRVERREISLKTFPVLAKTDPLAEY
                     LPPADGGGPKADDDFAGLY"
                     /name="TraM recognition domain-containing protein"
     gene            3264..3995
                     /gene="mobC"
                     /locus_tag="K6149_RS27845"
     CDS             3264..3995
                     /gene="mobC"
                     /locus_tag="K6149_RS27845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WP_071177728.1"
                     /translation="MTLEQYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRAKYPVDGRQVSVWGLTPHGVAFSFDEDEPLTDVIPFQP
                     SRVSAAQLPHRLAVQSLRLSMEGRGATGWRYLHRMALKGMKVPDALAELDGRTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRRANGIDEIWYICPDRPTLVRVQRAILAVDEIVNPQTGEA
                     RKTVELDRERLFACFKFMTTE"
                     /name="mobC"
     gene            4002..4532
                     /locus_tag="K6149_RS27850"
     CDS             4002..4532
                     /locus_tag="K6149_RS27850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_071177729.1"
                     /translation="MALYMVFIEDVEDKDGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGENPQWERDKPTGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQINAMIEVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(4559..4738)
                     /locus_tag="K6149_RS27855"
     CDS             complement(4559..4738)
                     /locus_tag="K6149_RS27855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165970.1"
                     /translation="MTKQEKTALNMAKFIKDQSLLLLEKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(4764..5192)
                     /locus_tag="K6149_RS27860"
     CDS             complement(4764..5192)
                     /locus_tag="K6149_RS27860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001140599.1"
                     /translation="MAKTLLNLSQAAQAAGITRRTLYNHVKQGKVTVSRDGKNNPVVDV
                     SELIRVYGNVNIPEKQIPGISHRENTQKNFPQEQLLAMQKELADLRQAVTLMLEDKTSR
                     EEERRQHDDERRKLQAEVDRLTTELTQKKKRFWSGWFS"
                     /name="hypothetical protein"
     gene            complement(5356..5670)
                     /locus_tag="K6149_RS27865"
     CDS             complement(5356..5670)
                     /locus_tag="K6149_RS27865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001136729.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]; GO:0009432 - SOS response [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_071177730.1"
                     /translation="MPQVTISALAQRDLQRLQDFLKTKNRLAARKGGEVIVRAIQQLKT
                     LPDIGRPVPFLPLEYKELVIGFGDSGYVMLYRHDREMDQIVIVTVRHQKESGYPGADSL
                     "
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(5658..5936)
                     /locus_tag="K6149_RS27870"
     CDS             complement(5658..5936)
                     /locus_tag="K6149_RS27870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014542884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family ribbon-helix-helix protein"
                     /protein_id="WP_000093040.1"
                     /translation="MSSATSIRLDEELKDRLKTLADDRHRSAHALMLEAITEYIDREEK
                     RSQYLRDGQAAWQHYQETGLHLTAEEAEAWISTWGTENEQDAPPCHR"
                     /name="CopG family ribbon-helix-helix protein"
     gene            complement(6111..6476)
                     /locus_tag="K6149_RS29090"
     CDS             complement(6111..6476)
                     /locus_tag="K6149_RS29090"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /GO_function="GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="WP_072354022.1"
                     /translation="MKAFTLTVLAAMFLTPVHAIPLKPVTTLQPTYPVQAAAKRIEGQV
                     TATFDILDDGRVDNVSLTGCHLFFRETLTAMRHWLYPAGHPAMRQVINFEFSLKNDGLQ
                     DPRPVTTQKLNDDGCRQ"
                     /name="TonB family protein"
     gene            complement(6473..6844)
                     /gene="tolA"
                     /locus_tag="K6149_RS27880"
     CDS             complement(6473..6844)
                     /gene="tolA"
                     /locus_tag="K6149_RS27880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237044.1"
                     /GO_component="GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA]"
                     /GO_function="GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="WP_001237044.1"
                     /translation="MRLIPFILLALCSPLLLTACKHSTTQPPKIYTLDKSSKISSYAAA
                     IKTAVEMQMFEPEAYAGKMCTVRISIQRDGTLNSAIAEGGDPKLCKAAISAVTHAKIPP
                     APDENTWQIFKNAPLDFRP"
                     /name="tolA"
     gene            complement(7118..7363)
                     /locus_tag="K6149_RS27885"
     CDS             complement(7118..7363)
                     /locus_tag="K6149_RS27885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032440458.1"
                     /translation="MTHLKLTPAEEKRLADAFVAQALAMGLKGNKTLPKAVKQQVRERV
                     LRAIQDEREARRAQEMRREKYEATETTFTWQPQRRR"
                     /name="hypothetical protein"
     gene            7690..9375
                     /locus_tag="K6149_RS27890"
     CDS             7690..9375
                     /locus_tag="K6149_RS27890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase domain-containing
                     protein"
                     /protein_id="WP_032440457.1"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSVSGDALSAAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPKMMSK
                     IMTSLPADAVTETPASTLPLDQATVRVRQRVVDVVKDERQHIAVVAGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPSLQVSVPKGVPTAKAPPKGIVAEKGDSRPAGFTAGGNSREAVIR
                     FPKETGQKPVYVSVTDVLTPAQVKQRQEEEKRRQQAWDAAHPEEGLKRDYDKAKAELDA
                     EDKNIATLNSRIASTEKALPGARAAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNEKLKAAQASLNAMNESLSRDKAALTGAMESRKQKEKKAKDAENKLNE
                     EKNKPRKGTKDYGHDYHPVPKTEDIKGLGELKRGDPKTPKQGGGGKRARWYGDKKRKIY
                     EWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPKRNIKKYL"
                     /name="colicin-like bacteriocin tRNase domain-containing
                     protein"
     gene            9385..9642
                     /locus_tag="K6149_RS27895"
     CDS             9385..9642
                     /locus_tag="K6149_RS27895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /GO_function="GO:0015643 - toxic substance binding
                     [Evidence IEA]"
                     /GO_process="GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E3-like toxin immunity protein"
                     /protein_id="WP_032440455.1"
                     /translation="MGLKLRLTWFDKKTEEFKGEEYSKDFGDDGSVIESLGMPLKDNIN
                     NGEFDLKKEWVPLLQPFFKNQIKPDVDNCYIAFDYRDGNW"
                     /name="colicin E3-like toxin immunity protein"
     gene            9727..9876
                     /locus_tag="K6149_RS27900"
     CDS             9727..9876
                     /locus_tag="K6149_RS27900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_032440454.1"
                     /translation="MKKVKTIFLFILIVAGFLLAACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     BGC             2690..10049
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          91..280
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="92.67"
                     /coverage="94.0"
                     /evalue="3.08e-71"
     Typing          3265..3992
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.416"
                     /coverage="100.0"
                     /evalue="1.34e-140"
ORIGIN
        1 tagccgcagt taggcaccca cttcaagacc tctcaatatc tctctcgtat atatccagtt
       61 accaatggct gctgccagtg gcgtttttgc gtgtccgtcc gggttggact caagatgata
      121 gttaccggaa agggcgcagc agtcgggctg aacggggggt tcgtgcatac agcccagctt
      181 ggagcgaacg acctaaaccg agccgagata ccagcagcgt gagctatgag aaagcgccac
      241 gcttcccgaa agggaaaaag gcggaacagg tatccggtaa acggcagggt cggaacagga
      301 gagcgcaaga gggagcgacc taccggaaac ggtggggatc tttaagtcct gtcgggtttc
      361 gcccgtactg tcagattcat ggttgagcct cacggctccc acagatgcac cgaaaaagcg
      421 tctgtttatg tgaactctgg caggagggcg gagcctatgg aaaaacgcca ccggcgcggc
      481 cctgccgttt tgcctcacat gttagtcccc tgcttatcca cggaatctgt gggtaacttt
      541 gtatgtgtcc gcagcgcccg ccgcagtctc acgcccggag cgtagcgacc gagtgagcga
      601 ggaggcgcaa tttccccggc acctgcaaga cggtcaccgg acgcgctgat gtgggatatt
      661 gtcccctgat taagtctttc tattccctga cgatcaatta tcaggatagg tgaaccatgt
      721 cggtttttcg ctgaacattt tacgttaacc tatctctgtt gcttttttct ccttgcggag
      781 aaatgcaagt catccccgct ggggcgtggc tgacttcagg ggaaaagacc ttgcttacgc
      841 tggctggacg ccaccgacgc acggtctttt cccctctttc tggctctgtc cggctggacg
      901 ccggccacgc cagaaattca cccggccccc gtcaggctca cgcctgacca cccacaaaaa
      961 tccacggtgc tggacgcacc tgacgaagca gcaaagctgc tcctgatttt gcgggtggtc
     1021 agccgctcgc tatggcgaac tctgacgggg gccggaacac cggaaaagac cgcactgggg
     1081 tgccgccttt tcctctcaca cgctggggcg tgctcgttgt tcctccgggg ctgcgccttt
     1141 tccgcatggg gcggaaagag gctggcctcc gcggtgaaag ctgaacgcaa tcaccacgga
     1201 cgtgaccgcc gccgccatgc cttggggcat gtccgcggct tgtgcagcgg gggctgcgca
     1261 tcacgccggt aaacccgtcg tctgaaggag agagattgca tgtttaatac ggattcgctt
     1321 gcctggcagt ggcatatccc ggagctggcc cggcagattt acccgtgggt cagctggctg
     1381 taccgggagt attacagtct ggccggtttt tttctggtta tcggcctgtt tttcggtctg
     1441 gctgaactct gggccggccg aatgcgtttt gtggggctga gccataaggc gcggcgggag
     1501 tatgccgatc gctataagcg cgataataac cggactgtgc cattttcggt gctggcctcg
     1561 ctggattcac ccgggcggat ggtcagggca cggcgacatg tctggaacta cgcgtactgg
     1621 ctgaccggcg ctgccggtct ggcccttctg ggggggctgg ttgtcgccgt gtttatcgag
     1681 agctggggag aaaccgtcct gcgcccggac ctctatgcga acgcagatta ttacctgctg
     1741 cagccggcta acctgttcgg gatagcctgg tgtgccggag ggcttgtcgc cggcgtcctt
     1801 ctggcgcggc tgctggcgtt cctgctgctt gatggtcatt ttgcggccgc tgacgaggcg
     1861 gtgaacgcga aactgaatca ggagagccgg cgcagcagcc agcggaccgg agaaatgaca
     1921 gacgtccgcc acctgcattt cggggagccg gtaccggtta acgcgctggc cgatttttcc
     1981 acggagcagg cccggaaaca gcaggcggtg tttctgggta aggatgagca gggccagccg
     2041 gtgctggtcc ctcgtgatac ctggcgcaag accaatattc agatcctcgg cctgcccggg
     2101 agcggcaaga gcgcgatggc caccaacgcg ctcattcgct gcgtacgcga tttcggggac
     2161 gctgtggtgt acttcgaccc caaaggggac gcgtgggcgc cgcacgtctt ccgggcgcac
     2221 tgcccggact tcaccctgct ggatctgcgg ccggggaaac cggcgcagct gaatctgttc
     2281 cgcgatctcg accagtacgc cctgaagaat ctgctggttg ccgggtttaa cctgagtgag
     2341 accggcgacg tggcggacca ctaccgcatc agcgagcaga aagcggcgaa gctgattgcg
     2401 gagcagttcc cgcagggggc aaacattcag caggtgctgg cggcggcgta cgcgttgccg
     2461 gaggccctga aaaaggacgt gaaggggctg atcaccaagc tggagaacgt ggccgacctg
     2521 agcgtcctgc agacggacag cggtatcgat gtggcgggga taatcaacgg cggcggctgc
     2581 ctgtacgtca tcggctcgat ggacgatgag gcggtgatcc gggtgcagaa gatgctgttt
     2641 gcccgctgcg cgcaaatcat cattgcgcgg gatgagttcc ggaggtggcc gcacgcgagc
     2701 atcatgctcg atgagattaa atatctgctg tcgaagtacg tgctgaacgc gctggggacg
     2761 ctgcgcagcc gggactgcaa cctgctgctc gcgcaccagt cgctgggcga cttcggtcag
     2821 tgcgggcagg atctgcccgc tgattttgtg aaaaccacgg tactggataa cacgccgatc
     2881 cgctggttct atcgcgcggc cagccaggag tccgcgcagt gggcggcggg ccagaccggg
     2941 gagatccggg tggacgtgga gcgccgccgg gccagccggg aggcggggaa cgtggagcac
     3001 atcagcggcg acagcttcat ccagaaggag gcgcggccgc tgtttgacgt gaacaccctg
     3061 caacacctgc ctgacgggtt cgcggtgatg accgggctgg gcgtggcgcg gctggcgttc
     3121 agcagcccgc tgcgggttga gcgccgggaa atttcgctga aaaccttccc ggtgctggcg
     3181 aaaacggacc cgctggcgga gtacctgcct ccggccgacg gcggcggacc aaaagcggat
     3241 gatgattttg cggggcttta ctgatgacac tggagcagta caacgtcagc cacgcgaagc
     3301 ggcaggcgcg gaacgcggag aaaacacggc tgaccctgcg ctggctgcgg gaagagctgt
     3361 gttccacggc ggagctggtt gcgcgacggc tggggattgc ggcggtacag ccggtttacc
     3421 gcttcctgga cagtctggtg gcgaaagggc tgctggtccg ggcgaaatac ccggttgacg
     3481 gccgtcaggt cagcgtgtgg gggctgacgc cgcacggggt ggcgttctcg ttcgacgagg
     3541 acgagccgct gacggacgtt attccgtttc agccgtcaag ggtgtcggcg gcgcagctgc
     3601 cgcaccggct ggccgtgcag tccctgcgcc tctccatgga gggccggggg gccaccggct
     3661 ggcggtacct gcaccgtatg gccctgaaag ggatgaaagt gccggacgcg ctggcggagc
     3721 tggacggcag aacggtggca tttgaggtag agcgcaccgt caagagccgc aggcggtatc
     3781 aggaagtggt gtccgggtat ctgttcaacc gccgggcgaa cggcatcgat gaaatctggt
     3841 acatctgccc tgaccggccg acgcttgtcc gggtacagcg ggcgattctg gcggtggatg
     3901 aaatcgtgaa tccacagacc ggcgaggccc gcaaaacggt ggagctggac cgggagaggc
     3961 tgttcgcgtg ctttaaattt atgacaacgg agtgagggat tatggcgctg tacatggtat
     4021 ttattgagga cgtggaagac aaagacggcg agcgctgcat tatgccaacc agcgagcatc
     4081 tggaagacat ggtggtgctc gcctggcaga ccggggcgta tgttttctgt tttgtcgacc
     4141 gggcccagca tctggcgatg atgaaatcca tcgggatgta tgacgagcgg ctcaacagcg
     4201 acgaaaaagc cacgtacttt ttcaccggtg aaaatccgca atgggaacgg gacaaaccca
     4261 caggaatgaa gctgtgtgat tacctctatc gaaaggtgga gcggttcgcc atcgagaacg
     4321 gtgtgcaggt ggttgatgcc gggaagtgct caccggagca gattaacgcg atgattgagg
     4381 tggtcagcga gcggcacggg gaaagcgcgg tgaacgagaa gctgaaagcg gagctgatgc
     4441 gggaatataa cagccgctat tttaacgtgc cggtgcctga actgccggag gacaaacccg
     4501 cccgccggtg gtggccgttc ggcaaaaaat aacgccagca ccggcatggc cggtgctgtt
     4561 attcttcccc gagcttatcc cgcatcacct gccagagtgt ttccgcatcc tcgtgaagcc
     4621 gttcgcacat gtcggccggg gtatccatgt ccagctcgtt cagcttttcc agcaacagca
     4681 gcgactgatc cttaatgaat ttagccatat tgagggcagt tttttcctgc ttcgtcatgt
     4741 tctccattcc cttcggtaag ttctcagcta aaccagccgg accagaagcg ttttttcttc
     4801 tgtgtcagtt ctgttgtcaa tctgtccact tcggcctgga gtttccggcg ctcatcatcg
     4861 tgctggcggc gttcttcttc ccgggatgtt ttatcttcca gcatcagggt gacagcctgc
     4921 cggagatcgg ccagttcctt ttgcattgcc agaagctgtt cctgtgggaa atttttttgt
     4981 gtattttcac ggtgtgaaat gccgggaatt tgtttctcag ggatattcac atttccgtat
     5041 acacggatca gttctgacac atccacgaca gggttgtttt tcccgtcacg tgacacggta
     5101 acctttccct gttttacatg attgtatagc gttcttctgg taatgcccgc ggcttgtgct
     5161 gcctgtgaaa ggttgagcaa tgttttcgcc actgtatacc cctgatttta tcgggtgtga
     5221 aaggtgtgta atttctcacg gtaaatgtat cgctgaattt tacccgtttt tccgttcagg
     5281 atgtggtttt ctgacgcgct gcgcttgtca aaccggcgac ggccacgcaa tgaattgcgc
     5341 ccccgtcatc ggttattaga ggctgtctgc ccccggatac ccggattctt tctgatgcct
     5401 gacggtgacg atcacaatct ggtccatttc ccggtcgtgg cggtagagca tcacatagcc
     5461 actgtcgcca aacccgatca ccagttcctt atattccagc ggcagaaacg gcaccgggcg
     5521 gccaatgtct ggcaatgttt tcagttgctg gatagcccgg acgatcacct cacctccttt
     5581 tctggctgcc agccgatttt tggttttgag aaagtcctga aggcgttgta aatcgcgctg
     5641 tgccagcgcg gaaatcgtta cctgtggcat ggcggagcat cctgctcgtt ctcagttccc
     5701 caggtactta tccaggcttc cgcttcttcc gcagtaaggt gcagacccgt ttcctgatag
     5761 tgctgccatg cggcctgtcc gtcccgcagg tactggctgc gcttctcttc ccggtcgatg
     5821 tattcggtga ttgcctccag cattagcgcg tgcgctgagc ggtgccggtc atcggccagc
     5881 gttttcagcc ggtccttaag ttcttcatcg agcctgatgg atgttgctga tgacataaaa
     5941 gcccctttgt agtcatatgt attacacatg actactttaa tccgtggcgg cgctatttgc
     6001 cagaaatacg gcttttgctg cccatttttc agcttcgctt ccactaatca gggctgctac
     6061 aggctcactc atcatccagg actattgcag cctgcgggtt aaaggacctg ttactggcgg
     6121 caaccatcat cattgagctt ttgggtggtg actggtcgcg gatcctgtaa gccgtcattt
     6181 ttcagactga attcaaaatt tataacctgt cgcattgccg ggtgccctgc gggatatagc
     6241 cagtgcctca ttgccgtcag cgtttcccga aaaaaaaggt gacagccggt aagcgacacg
     6301 ttgtccactc gtccatcgtc cagaatatca aacgtggcgg tcacctgacc ttcgatgcgt
     6361 tttgctgcgg cctgtaccgg gtaggttggc tgcagtgtgg ttacgggttt tagtggtatc
     6421 gcatgtaccg gagtgaggaa catggccgcc agcactgtca gagtaaaagc tttcatggtc
     6481 tgaaatccag aggggcgttt ttgaatattt gccaggtatt ttcatccggt gccggcggaa
     6541 ttttggcgtg ggtaacggct gatattgctg ctttacagag tttcggatct cctccttcag
     6601 caatggcgct gttcagagtg ccatcacgtt gtatggatat ccgcacggtg cacatttttc
     6661 ccgcataggc ctctggttca aacatctgca tttcaacagc ggtcttgatg gccgcagcgt
     6721 agctactgat tttagatgac ttatccagag tataaatttt cggcggctgg gtggtggaat
     6781 gcttacaggc cgtcaggagt aaggggctgc acaatgccag caggataaag ggtattaaac
     6841 gcatcgggtt gatgacctgt cagtaaaaaa tgttacatgt atatttgtca ctctttacgg
     6901 ccacgttcat ttacataaac acggcttatc gtatcgtttt tacaccaaac ggcccgccat
     6961 gcagttgcgt gcataggcat gcagcgggtt acaatttacc gggcgcgttt ccggcggttt
     7021 tccgggtggt ttgttgcctg ttttatcccg tagccgccgg aaacgccctc agaccgtctg
     7081 agcggtgcgg gcaatgcgac gttatggtaa atacccctta ccggcggcgc tgtggctgcc
     7141 aggtgaaggt ggtttccgta gcctcgtatt tctcccgacg catttcctgc gcccgcctgg
     7201 cttcacgttc gtcctgaatg gcacgaagca ccctttcccg cacctgttgt tttaccgcct
     7261 ttggcagcgt tttgttccct ttcaggccca tggccagtgc ctgagcgaca aaggcgtccg
     7321 ccaggcgctt ttcttcagcg ggagtcagtt tcaggtgcgt catagtggcc tccgggcgtc
     7381 tcaggtttcc cgaacagttt aaccgcaggt cctctcctca tgccagttag ctttcagatg
     7441 tgtctatacc atctgaaatg taagtgtgat gaatgtctta aataataaaa tttgttgcca
     7501 tgagaaattt tcgggaaaag acgcgatctg cttacaggtg gctccgggcg cttttttgtg
     7561 cctgaagatg agaactaatc tcatatattg acacctgaaa acgggaggag taaggtaata
     7621 attatactgt gtatatgtac agtatgttgg ctttaattta ttgttttaaa tgtcaaagag
     7681 gaaaacggta tgagtggtgg agacggtcga ggtccgggta attcaggtct gggacataat
     7741 ggtggtcagg ccagtgggaa tgtgaacggt acgtctggta aaggtggccc ttcatcaggt
     7801 ggtggtacgg atccaaacag cgggccgggc tggggtacga cgcatacgcc gaatggagat
     7861 attcataact ataatccggg ggagtttggt aacggcggga gtaaacccgg tggtaatggt
     7921 ggtaacagcg gcaatcatag cggtagctct ggtggcggac agtcttcggc cactgcgatg
     7981 gccttcggtc tgcctgccct ggctactccg ggggctgaag gactggcttt atcagtttcc
     8041 ggcgatgcgt tgtcggccgc cgttgctgat gtgctggctg ccctgaaggg gccgtttaag
     8101 tttggtctgt gggggattgc gatctacggt gtgctgcctt ctgagattgc aaaagatgat
     8161 ccgaaaatga tgtcaaaaat tatgacgtca ttaccggccg atgcggtgac ggagactccg
     8221 gcaagtactt taccactgga ccaggcgacg gttcgtgtca gacaacgggt tgtggatgtg
     8281 gtgaaggatg agcggcagca tattgcggtt gtcgcaggtc ggccaatgag tgtccctgtg
     8341 gtggatgcga aaccgacaaa acgtccgggg gtattcagtg tgtcgattcc gggtctcccg
     8401 tctctgcagg tgagcgtacc gaaaggtgtt ccgacagcga aagccccgcc aaaaggcatt
     8461 gttgctgaaa aaggtgattc acgtccggct ggttttacag ccggtggtaa ctcccgtgag
     8521 gccgttattc gtttcccgaa agagaccgga cagaagccgg tttatgtgtc ggtgacagat
     8581 gttcttaccc cggcacaggt aaaacagcgt caggaggaag aaaagcgtcg ccagcaggca
     8641 tgggacgccg ctcatccgga agaggggctg aaaagagact atgataaagc gaaagctgag
     8701 ctggatgccg aagataaaaa tattgcgacc ttaaacagcc gcattgcatc gacagagaag
     8761 gcgctccccg gtgcaagggc tgctgttcag gaagccgata aaaaggtaaa agaggcagag
     8821 gcaaataaag atgattttgt gacttataac ccgcctcatg aatatggctc cgggtggcag
     8881 gatcaggttc gctatcttga taaggatatt cagaatcaga atgagaaatt aaaagcggct
     8941 caggcatctt taaacgcaat gaatgaatcc ttatccagag ataaggctgc acttaccggg
     9001 gcgatggaga gccggaaaca aaaggagaaa aaagcgaagg atgcagaaaa taagttaaat
     9061 gaggaaaaga acaagcctcg caaggggact aaagactacg gccatgatta tcatccagta
     9121 cctaaaacag aagacataaa gggacttgga gagttaaagc gaggtgatcc taaaacacct
     9181 aaacaaggtg gtggtggtaa gcgggcccga tggtatggcg ataaaaaacg taaaatttat
     9241 gaatgggatt cccagcacgg tgagcttgaa gggtatcgtg ccagtgatgg cgaacaccta
     9301 ggcgcatttg atcccaaaac gggtaagcag gttaaagggc cagatccaaa acggaacatt
     9361 aaaaaatatc tttgagaggt aaatatgggg cttaaattac gtttgacatg gtttgataag
     9421 aaaactgaag agtttaaagg ggaagagtat tctaaagact ttggtgacga tggttctgtc
     9481 attgaaagct tgggaatgcc tttaaaggat aatattaata atggtgagtt tgatttgaag
     9541 aaagaatggg ttcctttact acagccattt tttaaaaatc aaataaaacc tgatgtggat
     9601 aattgttata tagcttttga ttatcgggac ggtaactggt aacaaaaaat tatcaaggat
     9661 gatttgaaat actgacttcc ggtgttcagg gatgaatgcc ggagtcttta aatacaggga
     9721 aagtatatga aaaaggtaaa gactattttt ctttttatat tgattgttgc tggctttctt
     9781 ctggccgcat gtcaggcaaa ttatatccgg gatgttcagg gcggaacggt ggcaccatcg
     9841 tcctcctctg aactgacggg gatcgcggtt cagtagaaaa gatcaaagga tcttcttgag
     9901 atcctttttt tctgcgcgta atctcttgca ctgtaaacga aaaaaccgcc tggggaggcg
     9961 gtttgatcga aggttaagtc agttggggaa ctgcttaacc gggtaactgg gtatacgaga
    10021 ccgcagatac caaaactgtt ctttcagtg
//
