LOCUS       NZ_CP081910             8943 bp    DNA     circular CON 30-MAR-2024
DEFINITION  Phocaeicola vulgatus strain BFG-191 plasmid unnamed2, complete
            sequence.
ACCESSION   NZ_CP081910
VERSION     NZ_CP081910.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN15547037
            Assembly: GCF_020091465.1
KEYWORDS    RefSeq.
SOURCE      Phocaeicola vulgatus
  ORGANISM  Phocaeicola vulgatus
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Bacteroidaceae;
            Phocaeicola.
REFERENCE   1  (bases 1 to 8943)
  AUTHORS   Tisza,M.J., Smith,D.N., Clark,A., Youn,J.-H., NISC Comparative
            Sequencing Program,N.I.H., Khil,P. and Dekker,J.P.
  TITLE     WGS Sequencing of Bacteroides fragilis Group Isolates
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8943)
  AUTHORS   Tisza,M.J., Smith,D.N., Clark,A., Youn,J.-H., NISC Comparative
            Sequencing Program,N.I.H., Khil,P. and Dekker,J.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2021) LCIM, National Institute of Allergy and
            Infectious Diseases, 9000 Rockville Pike, Bethesda, MD 20892, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP/FALCON v. v. 6.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 502x
            Sequencing Technology             :: PacBio Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_020091465.1-RS_2024_03_30
            Annotation Date                   :: 03/30/2024 06:22:11
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,480
            CDSs (total)                      :: 4,369
            Genes (coding)                    :: 4,241
            CDSs (with protein)               :: 4,241
            Genes (RNA)                       :: 111
            rRNAs                             :: 9, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 62 of 128
            Pseudo Genes (incomplete)         :: 77 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 25 of 128
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP081910.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..8943
                     /organism="Phocaeicola vulgatus"
                     /mol_type="genomic DNA"
                     /strain="BFG-191"
                     /isolation_source="Peritoneal Fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:821"
                     /plasmid="unnamed2"
                     /geo_loc_name="USA:Bethesda"
                     /lat_lon="39.0025 N 77.1044 W"
                     /collection_date="1987"
                     /collected_by="Department of Laboratory Medicine - NIH
                     Clinical Center"
     gene            join(8291..8943,1..325)
                     /locus_tag="K6V28_RS00195"
                     /old_locus_tag="K6V28_00195"
     CDS             join(8291..8943,1..325)
                     /locus_tag="K6V28_RS00195"
                     /old_locus_tag="K6V28_00195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008669318.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_008669318.1"
                     /translation="MEKKDKKQRDIVLSQDNALTTARYKFDLIEKRALYSIIRNIRAQY
                     IENENGRRDLFDDLIITIHEEELKKCGDNTKMQNIYDALKRLRNRDIEINNEKMWLSIG
                     FVNYVKHIKNKSYFEVQVSKEVLPYYVELAQNFTSYSMTVAIALKSTFSQRFYELCCQY
                     RNAGFFFYTVEKLREMFMLEKKYKRGCDFKRRVLDDPQKELRELYNAGQCDLYFEFEPK
                     SYKGKEVTEYKFYVYTRQTEQQKLLEYESAKQAIIYITTTISRFSKSDKGYVKRVVNAV
                     QLHPEIAVQVAQKIQKKFEQYIDKPAKQIGAIIRVCLMEDFGIE"
                     /name="replication initiation protein"
     gene            339..530
                     /locus_tag="K6V28_RS00200"
                     /old_locus_tag="K6V28_00200"
     CDS             339..530
                     /locus_tag="K6V28_RS00200"
                     /old_locus_tag="K6V28_00200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016274500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_021409502.1"
                     /translation="MKTGDKVIVPAEINGYGRDLRAIVTEIEKFAGAIFVTVIFTEPCP
                     EACGRGGGVYHDFQLIAR"
                     /name="hypothetical protein"
     gene            633..1154
                     /locus_tag="K6V28_RS00205"
                     /old_locus_tag="K6V28_00205"
     CDS             633..1154
                     /locus_tag="K6V28_RS00205"
                     /old_locus_tag="K6V28_00205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016271957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_008669313.1"
                     /translation="MEKGQESKQKRKTPLTTIAISQEYAKKLDEYLNGTGITRKEFVEL
                     SVDYFQRTGFDLRGEAFDLSPLEKVAGRLEQSAKIMERHNEGTEAVHLLLQAVREQTAK
                     QLPAPELIAHAAEEKAKAEAKSEEQEREIARLRSENSALLEYKEKAYRELCRIRDEQKT
                     IGKIKVNIEL"
                     /name="hypothetical protein"
     gene            1184..1726
                     /locus_tag="K6V28_RS00210"
                     /old_locus_tag="K6V28_00210"
     CDS             1184..1726
                     /locus_tag="K6V28_RS00210"
                     /old_locus_tag="K6V28_00210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005852953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="WP_080974082.1"
                     /translation="MKKFYLFILLPDYAAHEAVYFSQAITADDFAMKKNPKYVNKVVAP
                     TLEPISSIGGFRTLPDYSFDTMPDDYVALVLIGGFGWNTPVAEKVMPIIKSAIEQEKVI
                     GAICNAASFMAKCGIDRETTSKTLQKSSVIQKISVTLSSVIIAIIVCKLLIFNTLRLQQ
                     FFANHHFSGTYISSNSR"
                     /name="DJ-1/PfpI family protein"
     gene            1791..2846
                     /locus_tag="K6V28_RS00215"
                     /old_locus_tag="K6V28_00215"
     CDS             1791..2846
                     /locus_tag="K6V28_RS00215"
                     /old_locus_tag="K6V28_00215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008781140.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_215424929.1"
                     /translation="MIVMAMLAFFVFVSRYYVTCRNRQFEQSRWMIVVALLLFVVHYMC
                     QMRLGWRQQGNDVGVLFNLLFYSPSAILLSWSQLNILRAGHRRWSFMRYGVVGYALMVL
                     CIVAGVISNGSLHIGPMLYVADAIHFFTLLYYTWAPLRELGNVQRRLDSEHGNPADAYL
                     RTMRFGFFVVCAFAVISPLYILSRPLLFVFGPLGLISLSLFIVSFTALGFSMSEGVTEI
                     VTETNDEIAAAKVFREIGPERIAEIDMAVSKWRSEGGFRDSNLTLSSFARRIMVNRTDV
                     ASYLTSIYGKNFRTWLSGIRVEEAKALMAAHPEYSNEVVSMECGFSSRVYFQRLFKERT
                     GLTPAEWRRQR"
                     /name="helix-turn-helix domain-containing protein"
     gene            3066..4721
                     /locus_tag="K6V28_RS00220"
                     /old_locus_tag="K6V28_00220"
     CDS             3066..4721
                     /locus_tag="K6V28_RS00220"
                     /old_locus_tag="K6V28_00220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008669305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_149936476.1"
                     /translation="MQKNYLYLMVCVIGAMSLASCSEDSISPDAGGDGDGDSTTEVPLA
                     QVAKPKPNPWLAQEEYSITHFNSAQTDAFSSAVKDGTFNIDLSKCQMTWSGPVNLMTLA
                     STSPNYMWGMSSDRVSYLDISDGKLERVAEAGLPGITMKTQEQLTRIIADHATYDELSK
                     TVTDILGPAPQMSMANGNYVLCDKDNYAYTNAGQVMARYRLANPNNPKDGIMLDHQIRL
                     TNFTFGSFTLVGATMTYDGHLVVAAQNGLLVLNRALTTIEDSYPLPSDQILTNSICIDE
                     NGGVYVASNSRTPGGKGLMQKLICKDGKISTSQADGAWQAYYDGGPQAPCIKLGHGTGS
                     TPTLMGFGQDKDKLVVITDGSKRMKLVAFWRDEIPSDAQQVAGYDKRIAGVHEVTCGLG
                     TSTEWIQSEQSVVVGGYDAFVVNNINVTNQEINDKIIGVIAIGPIVKGPQGAECVRWNT
                     KEKKWESKWTRSDVSSVSMIPAVSIKSEMVFVCGWNDASGWEVTGLDWKSGATRHRSIL
                     GKNNRANGAYAIIQYLANGDLLFNSVAGPIRVKY"
                     /name="hypothetical protein"
     gene            complement(5276..6154)
                     /gene="btgB"
                     /locus_tag="K6V28_RS00225"
                     /old_locus_tag="K6V28_00225"
     CDS             complement(5276..6154)
                     /gene="btgB"
                     /locus_tag="K6V28_RS00225"
                     /old_locus_tag="K6V28_00225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007851393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein BtgB"
                     /protein_id="WP_008669303.1"
                     /translation="MNVKIQGSGNGTYANTGSCVAVTNYLQHEDLERMKKGEEVQPFFN
                     QFRDYVSAREVTFKIDNNKAKLSRTDAKFYVITVSPSEKELRCMGRTPQECAEALRRYI
                     RQDVMRNYAEGFGKGLKSDDVEYYAKIHFNRDGESDSDMHAHIIVSRKDRSNTRKLSPK
                     TNHTGKKNCGNVKGGFDRTDFFRKCESSFDRRMGFDREPEESFDYLNAVKNGSPAEIAR
                     QVERAERIRKEKWDKLKAELQSRQEPEKSKNQQVEKTPDIEQPIPKKKQQEEDLELLKK
                     PKRSRGFGMGM"
                     /name="btgB"
     gene            complement(6151..6756)
                     /gene="btgA"
                     /locus_tag="K6V28_RS00230"
                     /old_locus_tag="K6V28_00230"
     CDS             complement(6151..6756)
                     /gene="btgA"
                     /locus_tag="K6V28_RS00230"
                     /old_locus_tag="K6V28_00230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007851392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein BtgA"
                     /protein_id="WP_008669301.1"
                     /translation="MDKETTTSVTIDRKTFARLDRLAKSNNVSKKEFLSCALEYFEKYG
                     INPVEHESPAKEMQKLIKRCDQVIAFIRKQEQDFLRPACEAMGSTSMRVTMSMDSILTE
                     KKFSQYQKDNDLFMRDLASLAGIREQALDRAEKAVGQSRDMLLKNQQAIYARLDAVTQR
                     QEKIFSYIASYIDAKGKAGLFDDIKALYKNEKNKREKI"
                     /name="btgA"
     gene            7148..7495
                     /locus_tag="K6V28_RS00235"
                     /old_locus_tag="K6V28_00235"
     CDS             7148..7495
                     /locus_tag="K6V28_RS00235"
                     /old_locus_tag="K6V28_00235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007851390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_008669299.1"
                     /translation="MEAKSRFKVKLSNEAQEFLDGLPNKVREKIISNIRKSTFVLDPDL
                     FKKLDDTDIWEFRTFYNKTKYRLLAFWDKDDKINTLVIATHGFIKKTQKTPPKEIAKAE
                     EIRKDYFNSKR"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            7501..7815
                     /locus_tag="K6V28_RS00240"
                     /old_locus_tag="K6V28_00240"
     CDS             7501..7815
                     /locus_tag="K6V28_RS00240"
                     /old_locus_tag="K6V28_00240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007851388.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="WP_007851388.1"
                     /translation="MEAKKFYTLEEIEDKYIGEKGTPKRDAYEEELNSFLIGEAIKQAR
                     QSKNLTQEELGNLIGVQRAQISRIENGKNLTFSTIARVFKAMGISAKLEIGSMGKVALW
                     "
                     /name="helix-turn-helix transcriptional regulator"
     Typing          2..325
                     /name="rep_cluster_1097"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="6.850000000000001e-169"
     Typing          complement(5280..6154)
                     /name="MOBB"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 gttcgagcca aagagctaca aaggcaagga agttaccgaa tacaaattct acgtttacac
       61 aaggcaaacc gaacaacaaa agctattgga atacgaaagc gcaaaacagg cgatcatcta
      121 cataacaacc actatttcga ggttctctaa aagtgacaaa ggatatgtta agcgtgtagt
      181 gaacgccgta cagttacacc cggaaatagc cgtacaggtg gcgcaaaaga tacaaaagaa
      241 gtttgagcaa tatatagaca agccagccaa acagataggg gctatcatcc gggtttgctt
      301 aatggaagat tttggtatag aataaaaaac atacaagtat gaaaacaggt gataaagtta
      361 tcgtaccagc cgaaataaac ggctatggca gagatttacg ggcaatagtt acagagatag
      421 agaagttcgc cggggcaata ttcgtaacag tgatattcac cgaaccatgt ccggaagcct
      481 gcggaagagg ggggggtgtt taccatgact tccagctaat agcaaggtga tttattttgc
      541 tataatcgca ataaattgca ataatatctg tgataaattg ctataataat acttataact
      601 acctttgcaa cagaatatag cattttacag caatggagaa aggacaggaa agcaagcaaa
      661 agaggaaaac accgcttacc accatagcga taagccaaga gtacgccaaa aaattagacg
      721 agtatttaaa cggtacgggt ataactcgca aagaatttgt cgagttgtcc gttgattatt
      781 tccaaagaac cgggttcgac ctccggggtg aagcttttga cctctcccca ttagaaaagg
      841 ttgccgggcg gctggaacaa tccgcaaaga tcatggaacg acacaacgag gggacggaag
      901 cggtgcacct gcttctccaa gctgtccggg aacagaccgc caagcaactc ccagcgccgg
      961 aactgatcgc ccatgctgcc gaagaaaaag caaaggcaga agccaaaagc gaagaacaag
     1021 aaagggagat tgcccggctg cgctccgaaa actccgcttt gctggagtac aaagaaaagg
     1081 cataccggga actatgccgg atacgtgatg aacaaaaaac catagggaag ataaaagtaa
     1141 atatagagtt ataaaagaac atttataaat aaaaaaatca tgaataaaga aattttattt
     1201 atttatcctt ttgccggatt atgcagcaca tgaagctgtg tacttttcac aggctattac
     1261 agctgatgat tttgccatga aaaaaaatcc aaaatatgtc aataaggttg tcgcacccac
     1321 cttagaacca ataagttcga ttggtggatt ccgcacatta cccgattatt cgtttgacac
     1381 aatgcctgat gattatgtag cattggtatt aattggcggt tttggttgga atacacctgt
     1441 tgcagaaaag gttatgccaa ttattaaaag tgctattgaa caagaaaagg ttataggtgc
     1501 gatttgtaat gcagcttcat tcatggctaa gtgtggtatt gaccgagaaa caacatcaaa
     1561 aactttgcag aagtcatccg taatacagaa aatctcagta actttgtcct cggtaatcat
     1621 agcaattatt gtctgtaaac ttttgatatt taacactcta agattacaac aatttttcgc
     1681 taatcaccat ttttcaggca cttatatttc gtcgaactca cgttaaatta tttaatatac
     1741 atacaatctt ggatacttgc ttagacctta ttgctcttca atatacttgc atgattgtga
     1801 tggccatgct tgcatttttt gtgtttgtgt cgcgctacta tgtaacttgc cgtaacagac
     1861 agtttgagca gtcgcgttgg atgattgttg tggcattact attgtttgtt gtacactaca
     1921 tgtgtcagat gcggttgggt tggcgacaac aggggaacga tgtgggtgtt ctgtttaatc
     1981 tgctgtttta ttcgccttcg gctattttgt tgtcatggtc gcagcttaat attctgcgcg
     2041 ctgggcatag acgttggagt tttatgcgct atggcgttgt tggatatgct cttatggtat
     2101 tgtgcatagt cgcaggtgtt atttcaaatg gcagtctgca tattggtcct atgctatatg
     2161 ttgctgatgc tattcatttc tttactctat tgtattatac atgggcaccg ctgagggaac
     2221 ttggtaatgt tcaacgtcgg ctcgacagtg aacatggtaa tcctgcagat gcctacctgc
     2281 gcactatgcg ctttggtttt tttgtggtgt gtgcttttgc tgtaatttcg cctctttata
     2341 tattgtcgcg gccgctgctc tttgtctttg gacctttggg attgatttcg ctttcgctgt
     2401 ttattgttag ttttacggcg cttggattta gtatgtccga gggagtaact gagattgtta
     2461 ccgagactaa cgacgagata gccgctgcta aagtctttag ggagattggg cctgagcgta
     2521 ttgccgaaat agatatggcg gtaagcaaat ggcgaagcga gggcggtttt agagatagca
     2581 acttgacatt atcctcgttt gctcgtcgca taatggttaa tcgtaccgac gttgcttcgt
     2641 atctgacttc catatatggt aagaatttcc gtacatggct ttcgggtatt cgcgtggagg
     2701 aggcaaaggc actcatggct gcacaccccg aatatagtaa cgaggttgtt tctatggagt
     2761 gcggtttctc gtcgcgtgtg tattttcagc gtctgttcaa ggaaaggacg ggcttgactc
     2821 ccgccgagtg gcgccgtcaa cggtaatata ctggttatgt gtgcgtttgc tactgaatgt
     2881 aaggtgtaac ttttgtacga aggttacata ataaggcaaa gtgtaacctt tcttactgtg
     2941 acgtatcctt tctataagag tgacacacaa gtgatgtctt tgaggttttt gatggttgat
     3001 gctcctgtat ctttgcatga ggaaattcaa actattcaaa tataaattct caaaaacaat
     3061 tcactatgca aaaaaactat ctttatttaa tggtgtgtgt cataggggct atgtctttag
     3121 cttcgtgttc cgaagattct atttctcctg atgctggtgg cgatggcgat ggcgacagca
     3181 ctaccgaagt gcctttggca caagttgcaa aacccaaacc taacccttgg cttgcacagg
     3241 aagagtatag tattacgcac ttcaattcgg ctcagactga tgctttctcg tctgctgtca
     3301 aggatggaac tttcaatatc gatctttcca agtgtcagat gacatggagc ggtcctgtta
     3361 atttgatgac tcttgcttcg acttcgccca attatatgtg gggtatgagt agcgaccgcg
     3421 tgtcgtatct tgacataagc gacggcaaac ttgaaagggt tgccgaggct ggcttgccag
     3481 ggattacaat gaagacccag gagcaactga cacgtataat tgccgatcat gctacttacg
     3541 acgaactttc caagacggtt accgatatat tgggtccggc gccacaaatg tctatggcca
     3601 acggtaatta tgtcttgtgc gacaaggata actatgctta caccaatgca ggtcaggtaa
     3661 tggcacgcta tcgccttgcc aatcctaata atcccaagga tggtatcatg ttggatcatc
     3721 agataagact tacgaatttt accttcggct cttttacact cgtaggcgct actatgacct
     3781 atgacggaca ccttgttgtg gctgctcaga acggacttct tgtgctgaat cgtgctctta
     3841 ctacaatcga agattcatac cctcttccat cagaccagat tctcaccaac tctatctgca
     3901 tcgacgagaa tggcggtgtt tacgtagcaa gtaacagccg cacaccaggc ggaaaaggcc
     3961 ttatgcagaa actcatctgc aaggacggca aaatctccac ctcccaggct gatggtgcat
     4021 ggcaagccta ttacgacggt ggtccgcaag caccatgtat caaactcgga catggcactg
     4081 gttcaacgcc tacacttatg ggctttggcc aagacaagga caagctggta gttattaccg
     4141 atggctctaa gcgtatgaaa ctggtagctt tctggcgtga cgaaatcccc tcagacgcac
     4201 agcaagtggc aggatacgac aagcgtatag ccggagtgca cgaggttaca tgtggtcttg
     4261 gaacatcaac agagtggata cagtctgagc agtctgttgt agtaggcggc tatgacgcct
     4321 ttgttgtaaa caatattaat gttaccaatc aggaaataaa cgacaagatt attggtgtaa
     4381 tcgctattgg tcctattgtc aaaggccctc aaggtgccga gtgtgtacgt tggaatacaa
     4441 aggagaaaaa gtgggagagt aagtggacgc gcagcgatgt aagttctgta agtatgattc
     4501 ctgccgtaag tatcaagtca gagatggtat ttgtatgtgg ctggaacgat gcttcgggct
     4561 gggaagttac aggacttgac tggaagagtg gtgccacacg tcaccgcagc atattgggca
     4621 agaacaaccg agccaatggt gcctacgcca tcattcagta tcttgctaac ggcgaccttt
     4681 tattcaactc tgttgccgga ccaatccgcg taaaatattg agataattta atcatcatat
     4741 aacagttatg tcaatacttc actaaaaaat taccgaagta ttgtaatgac acacccgaac
     4801 atattgaaat gttctatcag ttccataaac aaggcttctg ttctttattc ctagatacag
     4861 gtgataaata acagataaga aacagattat aacaagcctc cggcggggtt ttcgctgggg
     4921 gcttttgacc tttgcacata taaaaagcaa aaagaaagcg atatcggagc gtataccata
     4981 aaacagacca ccgactatta caaaacgata ttacgtgcct aactgatcgg ggcagttcct
     5041 ccaccatgcg gacaaatccc ggtaacaaaa tgacaaaacg gacactcacc aaagaacaac
     5101 atttttttct tttttcctaa acaccttcca gcgaagcaca gtgtatttgt caagggcgaa
     5161 gggtaaaaaa taccgcacga gcgaagcgag cacaggagat tttttacccg gagagtgaaa
     5221 cgtcccttta caaatgcggc gtgcggagtt acctttgtgc cggaaaatag aaaaatcaca
     5281 tgcccattcc gaaacctctg ctcctttttg gcttcttcag gagttccaga tcttcctcct
     5341 gctgcttctt tttgggaata ggctgctcga tgtccggcgt tttctccacc tgttgatttt
     5401 tcgacttctc cggctcctgc cgggattgaa gctctgcttt gagtttgtcc catttctcct
     5461 ttctgatgcg ttccgcacgt tccacctgcc gggctatttc cgccggactt ccgttcttga
     5521 cggcattaag atagtcgaag ctttcctccg gctcccggtc aaaccccatg cgccgatcaa
     5581 aagagctttc acacttccgg aaaaaatccg tccggtcaaa acctcccttc acgttgccgc
     5641 agttcttctt cccggtgtgg ttcgttttag ggcttaactt cctggtgttg ctccggtctt
     5701 tccggctgac gatgatatgt gcgtgcatat cgctgtcact ttcaccgtcc cggttgaaat
     5761 ggatctttgc ataatattcc acatcatcgc ttttcaagcc ttttccgaag ccctccgcat
     5821 agtttctcat cacatcctgc cgtatgtacc gccgtaaggc ttccgcacac tcctgcggag
     5881 ttctgcccat gcagcgcagc tccttctctg aagggctgac agtgataaca tagaactttg
     5941 cgtcagtccg gcttaactta gccttgttgt tgtctatctt gaatgtgacc tcccttgcgc
     6001 tcacgtagtc ccggaactgg ttgaagaacg gctgcacctc ctcccctttc ttcatcctct
     6061 ccaaatcctc atgctggaga tagttcgtca ccgcaacaca actgcctgta ttggcatatg
     6121 tgccgttacc gcttccctgt atcttcacgt tcatatcttt tcccttttgt tcttctcgtt
     6181 cttgtacaag gcttttatat catcgaaaag cccggctttt cctttcgcat cgatatagct
     6241 tgcgatatag gagaagatct tctcctgcct ttgcgtcaca gcgtccagcc ttgcataaat
     6301 ggcttgttgg ttcttcagaa gcatatccct cgactgaccg actgcctttt ccgcccggtc
     6361 caaagcctgc tcccttatcc cggcaaggct tgcaaggtca cgcatgaata ggtcattgtc
     6421 tttttggtat tggctgaact ttttttccgt aagtatcgag tccatgctca tggtcaccct
     6481 catacttgta ctgcccatgg cttcacaagc cggacgcaag aaatcctgct cctgcttccg
     6541 gatgaacgct atcacctgat cacagcgttt gataagtttc tgcatttcct ttgccggact
     6601 ctcatgctca accgggttga tgccgtactt ctcgaagtac tccagcgcac aggaaaggaa
     6661 ctctttcttt gatacattat tggactttgc cagcctatca agccgggcaa acgtttttcg
     6721 gtcaatggtg acagaagtgg tggtttcctt atccatacaa ttgcggtttt aattgctgtt
     6781 tatattatta aatctctgat ttccaaaggt atgattttaa ccacaatttc aaactaattg
     6841 cggttaaaaa acttaaacgg ctgatattca gccgtaaccc cgtagggcaa gaggaaaaac
     6901 agggtaaagc ccggtttcct cttgctgttc tttcgactgc cgagcaatcg acgaacaccc
     6961 tcccctgcgg tacggcgaaa gctcgtacaa gggggacggg gagaagtcgg agcgaggcac
     7021 aaagaaagaa cctattccac aaacaaaaaa acacatcaaa acggcacaac aggctgcata
     7081 aaatatcaat attttgtttc gctatatgga aacaaaatat tatctttgca acactaaaaa
     7141 actttatatg gaagcaaaat caagattcaa ggtcaaatta tccaacgaag cccaagagtt
     7201 tttagacgga ttgcccaaca aggtacgtga gaagatcatt agcaatattc gtaaatccac
     7261 ctttgtactt gatccagatt tattcaagaa attggatgat acggatattt gggaattcag
     7321 aacgttctat aacaaaacca agtacaggct tcttgctttt tgggataagg acgataagat
     7381 aaacactctg gtaatcgcaa cacatgggtt tatcaagaaa acccaaaaga cacctcccaa
     7441 agaaatagcc aaagccgaag agattagaaa agattatttt aattcaaaaa gataaagcgt
     7501 atggaagcaa agaaatttta tacactcgaa gaaatcgagg ataaatacat cggagaaaaa
     7561 ggtactccca aacgtgatgc ctacgaagaa gaacttaact cttttttgat aggtgaagcc
     7621 attaaacaag ctcgccaatc aaagaactta acccaagagg aattaggtaa tctgatagga
     7681 gtgcaacggg ctcaaatctc ccgaattgaa aacggtaaga atttaacctt ttccaccatt
     7741 gcaagagttt ttaaggctat ggggataagt gctaaacttg aaatcggaag catgggtaaa
     7801 gttgcgttat ggtaactaac tatacaacaa aaagttacat taaatccggg atgcgttttc
     7861 gctcccggaa acttctcgtt ttatacatta aaagtcatat ttatacaaaa tttaacacgc
     7921 caaaaaatct tcctaatccg ttgcacttgg ctttacttgt ttccactctc tcccattctc
     7981 cggaaattcg tcgtatcaaa aaacacgctg aaaagtggag cgaagcgacc tctataaacc
     8041 aaaaaagaaa cactccattg ctttacggta atttttagtt tctttttagt tacttttacg
     8101 gcatagcata acacattgtg ctgtaatatc ttacaaaggt catctacgac aaatttccga
     8161 gcatctacga caaatttccg agcatctacg acaaatttcc gaaaaaaacg gttatctacg
     8221 acaaatattc tacgccatat atttgttttt gtcgtagata aatatttcct ttgcgaaaag
     8281 cattaaagtc atggagaaga aagacaagaa acagagggat atagtcctgt cgcaagacaa
     8341 tgcgctgact accgcacgct acaagttcga ccttatcgaa aaacgtgccc tatactccat
     8401 tatacgaaat atccgggcgc aatacatcga gaacgagaac ggcagacggg acttattcga
     8461 tgatctgatt atcaccatac acgaggaaga actaaagaaa tgcggtgaca ataccaagat
     8521 gcaaaacatc tacgatgcgc taaaacggct tagaaatcgc gatatagaga ttaacaacga
     8581 aaaaatgtgg ctaagtatcg gttttgtcaa ttacgtcaag catatcaaaa ataaatcgta
     8641 ttttgaggtg caagtttcca aagaggtact accctattac gtggaactcg cccaaaactt
     8701 cacgagttac tccatgacgg tagcaatcgc actcaaaagc acattctcgc aaaggtttta
     8761 cgaactgtgc tgccaatacc gaaacgccgg gttctttttc tacacagtcg aaaagcttcg
     8821 ggaaatgttc atgttggaaa agaaatacaa acggggatgc gatttcaaaa ggcgtgtact
     8881 tgacgatccg caaaaggaac tccgggagtt gtacaatgcc ggacaatgcg atttatactt
     8941 cga
//
