LOCUS       NZ_CP081886             4073 bp    DNA     linear   CON 09-OCT-2023
DEFINITION  Escherichia coli strain WW2 plasmid unnamed5.
ACCESSION   NZ_CP081886
VERSION     NZ_CP081886.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN20855217
            Assembly: GCF_019802685.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4073)
  AUTHORS   Wan,C., Liu,X., Tang,R., Fu,L., Wang,L., Wang,P., Yu,Y. and Wu,C.
  TITLE     Nano-TiO2 mediated antibiotic resistance gene transformation in
            activated sludge: Phenomenon, mechanism and potential risk
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4073)
  AUTHORS   Wan,C., Liu,X., Tang,R., Fu,L., Wang,L., Wang,P., Yu,Y. and Wu,C.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-AUG-2021) Department of Environmental Science and
            Engineering, Fudan University, No. 220 Handan Road, Yangpu District,
            Shanghai, Shanghai 200438, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 700.0x
            Sequencing Technology             :: Illumina; PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 10/09/2023 02:48:27
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,068
            CDSs (total)                      :: 4,953
            Genes (coding)                    :: 4,805
            CDSs (with protein)               :: 4,805
            Genes (RNA)                       :: 115
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 52 of 148
            Pseudo Genes (incomplete)         :: 103 of 148
            Pseudo Genes (internal stop)      :: 26 of 148
            Pseudo Genes (multiple problems)  :: 29 of 148
            Pseudo Genes (short protein)      :: 4 of 148
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP081886.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..4073
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="WW2"
                     /isolation_source="sludge from the aeration tanks of urban
                     wastewater treatment plants"
                     /db_xref="taxon:562"
                     /plasmid="unnamed5"
                     /geo_loc_name="China:shanghai"
                     /collection_date="2020-10-04"
     gene            <1..1520
                     /locus_tag="K5O11_RS25380"
     CDS             <1..1520
                     /locus_tag="K5O11_RS25380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016240513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="WP_261399653.1"
                     /translation="ETRGIETERGEQNRLINRLNLEIQISRTELRNGGLTEPARRVADL
                     LNIGLPDNATSYTLRDIIEALPKDSNSAWKLTSNAMSMMADMQATRRRWEELNTERKEA
                     MSHAASLKKSSPFSSGFSRIPLMQWAAPEYRSEQGKIQSLSQQMEKLRQHYRKVEKQDI
                     PASQMAFEKQWQQWGKSGLAALRAQLLKREEELRRKEQEDTERSRAEQLRKNDNAVMDS
                     GVLRAVVTGYGEAPMPGNGEMTCYLLLHNRNGEYTLWGNELEKYRTRIFESVDLMRDRS
                     GYICDRSEIGQRPPLQRVYSSATFEQLLTQVCQTWPQHTRNLRQPKTWPESFCLGEDRQ
                     PAMPSPAARKVDFMQGRLPPTLMPVMSSVDRETRQLQLLLVMGVDDSLGGVIRLNGTLY
                     PAFAVPSADNRHLVISALTDKGLRYAGYGVAVNHDADSHISPAPELMQFHLKTREAPLF
                     AAVNTPEKQPDHLFRSLGFNRTWEEWQREEDARTHTTERRHDRGWSQ"
                     /name="conjugal transfer protein"
     gene            complement(1778..2479)
                     /locus_tag="K5O11_RS23880"
                     /old_locus_tag="K5O11_23875"
     CDS             complement(1778..2479)
                     /locus_tag="K5O11_RS23880"
                     /old_locus_tag="K5O11_23875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001281399.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_001281395.1"
                     /translation="MSDLIAYKSNALIEASYKLTLQEQRFLLVCISRLKSGEDSPSPDE
                     QKTMTITAAEFHDAFPDMGRQHAEARLKEAIDRLWDRSVIIKNEEKREEFRWVQYRAQY
                     FKGEAKVDITFSDAIMPYLTQLKGQFTRVVVKNVSSLSSTYSIRIYELLQQFRSTGDRT
                     IAIDDFRTMLAVEDKYQQFKDLNKMIIKPAIAELNEKSDLVVTVETIKKGRTVVALHFR
                     FKEDKQIKMTI"
                     /name="replication initiation protein"
     gene            complement(3013..3267)
                     /gene="mobC"
                     /locus_tag="K5O11_RS23885"
                     /old_locus_tag="K5O11_23880"
     CDS             complement(3013..3267)
                     /gene="mobC"
                     /locus_tag="K5O11_RS23885"
                     /old_locus_tag="K5O11_23880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase accessory protein MobC"
                     /protein_id="WP_000091746.1"
                     /translation="MSRTLEQKIADAEARLQRLKAKSRSLDTAQKVVVGAALLAKVRKP
                     EEVQLRAWLLQFLKAEVTRQADVTRILPLINELEALPEQ"
                     /name="mobC"
     gene            3446..>4073
                     /gene="mobQ"
                     /locus_tag="K5O11_RS23890"
                     /old_locus_tag="K5O11_23885"
     CDS             3446..>4073
                     /gene="mobQ"
                     /locus_tag="K5O11_RS23890"
                     /old_locus_tag="K5O11_23885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobQ family relaxase"
                     /protein_id="WP_222563287.1"
                     /translation="MAIYHLSMKIISRSSGYSAVASAAYRSGSLMLDERTGLTHDYTRK
                     SGVAEAVILTPATAPAWCTNRAELWNAVEKAERRKNSQLAREIELAIPHELPQDAARET
                     VLAFVRENFVSQGMIADVAFHHMDKTNPHAHIMLTTRAIGPAGFSGKVRDWNDRTHAET
                     WRASWADHANRALANAGYQEEIDHRSYERQGLEKTPGIHLGKSACA"
                     /name="mobQ"
     Typing          complement(3269..3445)
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="5.61e-88"
     Typing          3446..3804
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(1779..2479)
                     /name="rep_cluster_1778"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          4..1517
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="95.285"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          3360..4072
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="97.479"
                     /coverage="99.0"
                     /evalue="1.45e-153"
ORIGIN
        1 tggaaacacg cggtatcgaa acagaacgcg gcgaacagaa tcgtctgatt aacaggctta
       61 acctggaaat acagatttcc cgcaccgaac tcaggaacgg agggctgaca gagcctgccc
      121 gccgtgtggc tgaccttctg aatatcgggc ttcctgacaa cgcaacttcg tacacactac
      181 gtgacattat cgaagcgttg ccgaaggaca gtaactcggc atggaaactc accagtaatg
      241 ccatgtcgat gatggcagat atgcaggcga cgcgcagacg ctgggaagag ctgaacaccg
      301 aacgaaagga agcgatgagc catgctgcca gcctgaaaaa atcatcgccg ttttcgtccg
      361 gtttttcccg tattcccctg atgcaatggg cggcaccgga ataccgtagt gagcagggaa
      421 aaatccagtc tctgagccag caaatggaaa aattacgaca gcattaccgg aaggtggaga
      481 aacaggacat tcccgcgagt cagatggcat ttgaaaaaca gtggcagcag tgggggaaat
      541 ccggccttgc agctctcaga gcccagttgc ttaaacgtga agaggaattg cgccggaagg
      601 aacaggaaga tactgaacgt agtcgcgcgg agcagctcag gaaaaatgac aatgccgtta
      661 tggattcagg ggtgctgaga gcagtcgtta cggggtatgg cgaggctccc atgcctggta
      721 acggggaaat gacctgttac cttctgcttc acaaccggaa cggagaatat acgctctggg
      781 ggaacgagct ggagaaatac cgcaccagaa tctttgagag tgttgattta atgcgtgaca
      841 ggagtggtta tatctgtgac cgaagcgaaa tcggccaacg tccgccactg caacgtgttt
      901 attcctctgc aacctttgag caactgctga cgcaggtttg ccagacatgg ccgcaacata
      961 cccgcaatct ccgccagccg aagacatggc cggagtcttt ctgtcttgga gaagaccgcc
     1021 agccagccat gccatcacca gcggcaagga aggtggattt tatgcaggga cgtctgccac
     1081 cgacgctgat gccggtcatg agttctgtcg accgggaaac ccgccagctt cagcttttgc
     1141 tggtcatggg cgtggatgac tctctgggcg gtgttatccg gcttaatggt acgctgtatc
     1201 ccgcgtttgc cgttccctca gccgataaca ggcatctggt catcagtgcg ctgaccgata
     1261 agggattacg gtatgcaggg tatggtgttg ccgtaaatca cgatgcagac agccacatat
     1321 cacctgcacc agaactgatg cagtttcatc tgaaaacccg tgaggcaccg ctttttgctg
     1381 ccgtcaacac accagaaaaa cagccggatc atctgttccg ctcgctgggt tttaatcgta
     1441 cctgggagga atggcagcgc gaggaagatg cacgaacaca caccaccgag aggagacatg
     1501 atcgcggttg gtcacagtga atgtgaacaa tattttgttt tgtttaacat cgtgttatgt
     1561 tcttcgatgt tcttcgatgt tcatcgacag ggatgaatgc aagaagcaca ctcacataac
     1621 gcaagcatag ttttgttttt ccgcccccaa aaattggggg gtcttttttc gctgcatcag
     1681 agcgtttttg acgcaatttt tcacttcaat agtaccttcg cactactaac acaaaaaaaa
     1741 cgcagcaggg agcgttttag gtgccttaaa atcgatttta gatcgtcatc ttgatctgtt
     1801 tgtcttcctt gaagcgaaaa tgcagggcta caaccgtgcg cccctttttg atcgtctcaa
     1861 cagttacaac cagatcgctt ttttcgttca gttcggcgat ggctggcttg attatcatct
     1921 tattcaagtc tttgaattgt tgatatttat cttcaacggc aagcatggtt cggaaatcat
     1981 cgatagctat cgttctgtca ccggtgctgc ggaactgctg gagcagctcg taaatgcgga
     2041 tgctgtaggt gctggaaagg ctggagacgt tcttcacgac tacccgcgta aactgcccct
     2101 tgagctgtgt cagatacggc atgatggcat cggagaaggt gatatctact ttcgcctcac
     2161 ctttgaaata ctgcgcccgg tactgcaccc agcggaactc ttctcgtttt tcctcgttct
     2221 tgatgattac tgacctgtcc cagaggcgat ctatcgcttc cttgagccta gcttcagcgt
     2281 gctgccgtcc catgtcagga aaggcatcat gaaactctgc agccgtgatg gtcatggttt
     2341 tttgttcatc cggcgaaggg gaatcttctc cggattttag gcggctgata cagaccagca
     2401 ggaagcgctg ttcctgcaaa gtgagcttgt agcttgcctc gataagggcg tttgatttgt
     2461 aagctatcag atctgacatt tcgcttgctc gttgctattg gtggcgtttt tttactgtac
     2521 gccacttatc atatgggtgg aagttttttt tttgcaaccc cctttttctg tggattacac
     2581 ccccaaaagc tccacctttc acccccaaaa gctccacctt tcacccccaa aagctccacc
     2641 tttcaccccc aaaagctcca cttatcttgc cagaacatct ttaaaatcat ggtgttacaa
     2701 gacctaaaaa ggttttaaaa aaaaagatct aaaaataaga tcttaaaaac ttaacggcga
     2761 gcctgtggat aacttggggt gctgaaaaca aaaaaccaat aaaaacaatg cgttgcaata
     2821 aagcagaatt gcaagtttcg aatacacccc ctccaaccct gtggaaaccc agtattcatg
     2881 cgggttcagc gcatatcacc tgccgaaaaa ccgtgcgtat gtgctcaacc tggcgactga
     2941 ttcacttccg cttcctcgcc cactcgctcg ctaacgctcg gtcgctgggg ctgcggcgat
     3001 cggacgatac aatcactgtt ctggcagcgc ctccagctcg ttaatcagcg gcagtatgcg
     3061 cgtcacatca gcctgtcgtg tcacctctgc cttcaggaac tggagtaacc aggcacgcaa
     3121 ctgcacctcc tccggctttc tgacctttgc cagcaatgca gcacccacaa caaccttctg
     3181 cgctgtgtcc aggctccggc tcttcgcctt caggcgctgt aatctcgcct cagcatcagc
     3241 aatcttctgt tcgagcgttc tgctcatgat gtaactccgt gcgcgatgaa aaatcgcatt
     3301 ttagcgcgtc actggtagtt taaaaactga gctggcataa tgcacggcaa atcacgaagt
     3361 gcgcacttat acaatctcca ctccgtttcg attgtgtgcg ccctgcgggg ctgaaagaaa
     3421 actacaaaaa ggcattacgg cagaaatggc gatttatcat ctcagcatga aaattatctc
     3481 cagaagcagc ggctacagcg ccgtagcttc cgctgcctat cgttccggtt cactgatgct
     3541 tgatgagcgt accggactga ctcacgacta cacccgcaaa agcggtgtgg ctgaggctgt
     3601 gattctgacc cccgccaccg cccctgcctg gtgtacgaac cgtgccgaac tctggaatgc
     3661 cgttgagaag gccgaacgcc ggaagaactc acaacttgcg cgggaaattg aactcgccat
     3721 cccccacgaa ctgccgcagg atgccgcacg ggaaaccgtt ctcgcctttg tcagagaaaa
     3781 ctttgtcagt cagggcatga ttgctgatgt ggcgttccat cacatggata aaacaaatcc
     3841 ccatgcgcac atcatgctga ccacgagggc tatcggacct gctgggttca gcggaaaggt
     3901 cagggactgg aacgaccgga cacatgccga gacgtggcgg gcatcatggg ctgaccatgc
     3961 aaacagggca ctggcgaacg ccggttatca ggaagagata gaccatcgtt catacgagcg
     4021 acaggggctt gagaaaacgc ctggtattca cctcggaaag tctgcctgtg cga
//
