LOCUS       NZ_CP081884             2097 bp    DNA     linear   CON 09-OCT-2023
DEFINITION  Escherichia coli strain WW2 plasmid unnamed7.
ACCESSION   NZ_CP081884
VERSION     NZ_CP081884.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN20855217
            Assembly: GCF_019802685.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2097)
  AUTHORS   Wan,C., Liu,X., Tang,R., Fu,L., Wang,L., Wang,P., Yu,Y. and Wu,C.
  TITLE     Nano-TiO2 mediated antibiotic resistance gene transformation in
            activated sludge: Phenomenon, mechanism and potential risk
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2097)
  AUTHORS   Wan,C., Liu,X., Tang,R., Fu,L., Wang,L., Wang,P., Yu,Y. and Wu,C.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-AUG-2021) Department of Environmental Science and
            Engineering, Fudan University, No. 220 Handan Road, Yangpu District,
            Shanghai, Shanghai 200438, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 700.0x
            Sequencing Technology             :: Illumina; PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 10/09/2023 02:48:27
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,068
            CDSs (total)                      :: 4,953
            Genes (coding)                    :: 4,805
            CDSs (with protein)               :: 4,805
            Genes (RNA)                       :: 115
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 52 of 148
            Pseudo Genes (incomplete)         :: 103 of 148
            Pseudo Genes (internal stop)      :: 26 of 148
            Pseudo Genes (multiple problems)  :: 29 of 148
            Pseudo Genes (short protein)      :: 4 of 148
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP081884.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..2097
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="WW2"
                     /isolation_source="sludge from the aeration tanks of urban
                     wastewater treatment plants"
                     /db_xref="taxon:562"
                     /plasmid="unnamed7"
                     /geo_loc_name="China:shanghai"
                     /collection_date="2020-10-04"
     gene            <1..173
                     /locus_tag="K5O11_RS25365"
                     /pseudo=""
     CDS             <1..173
                     /locus_tag="K5O11_RS25365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001362017.1"
                     /note="incomplete; too short partial abutting assembly gap;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=3
                     /transl_table=11
                     /product="replication protein"
                     /name="replication protein"
     gene            1281..>2097
                     /locus_tag="K5O11_RS23480"
                     /old_locus_tag="K5O11_23475"
     CDS             1281..>2097
                     /locus_tag="K5O11_RS23480"
                     /old_locus_tag="K5O11_23475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001362017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="WP_075816467.1"
                     /translation="MKNPDLQNLTDYSPSDAPWDAHRSVSDDVGGIYLLAAEYERYGAR
                     MALCGGLLRFGWSTLKETGETRLRLREAHFCRVRHCPVCQWRRSLMWQARFYQSLPRIV
                     ADYPDARWMFLTLTVRNCAIGELGEMLNRMNAAFQRMKVRKEFRPVQGWIRTTEVTRGS
                     DGSAHPHFHTLMMVPPGMLNGKSYVRHERWVELWRECLRVDYDPNVDVRAVKPRKPKGG
                     ESLACATAELVRGAVAETLKYSTKPADMVADPEWFLELTRQTHKRRFVAT"
                     /name="protein rep"
     Typing          176..257
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="93.976"
                     /coverage="90.0"
                     /evalue="3.4000000000000004e-27"
     Typing          complement(2..91)
                     /name="ColpVC"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="97.802"
                     /coverage="100.0"
                     /evalue="3.09e-36"
ORIGIN
        1 gcggcgcact caaagacgtg ctgaaactcg atcaggaaac tgacgccgac atggtgatcg
       61 gtgatgatat ttccgagggc gacgatgacg gttcacgaat cgcgttcgag tggaaaaccg
      121 aggtcagaaa gtaccgtcgc tcaccgacga aagataaaac tgaatcaggc tagcggggtg
      181 tcggggtgca cccctgacca gatggcaatt gtgatagcgt cgcgtgtgac ggtattacaa
      241 ttacacatcc tgtcccgaaa attcccgccg tttctcggtt ttttgagcgt cgcagaccgc
      301 acgacaaacg aacctaaggt gagtttgtat aagtgcgcac ttcgtgagat tccctgactg
      361 ccagtttgtc gattgcgcgg aacggctgcg gcacgaaaac ggcaaaatga tttactcgcc
      421 gtttgtgtgg gttgttttgt ggggtgtcaa tggggttgtg gtcttttttg tgggttaaag
      481 tgtgggtgat tgtgtgggct gtgtgttttg agttgtgggt gattatgtgg gtcattgtgt
      541 gggctgtatg cacggcattt ttttgttctc ctaaaacaca ggcacgataa gcggcgtaca
      601 caaaacgggg cgtttcgctc cgtcgcttgt gccaaagggg acaccccttt gcaaccctgt
      661 gcggttctac gaacctggtc aggactgcgc cccgacaccc cgccaacgcc tcacggaacg
      721 cgaaaaaatt ccgcgtaccg ttcaccgttc cctgattcgt caaacagaaa atcgcggggg
      781 cccgcgcctc cggcgtgggt tccgattttg tgattgacga cggcacggga aaaacactgg
      841 ggatggtggt tggtgtcacg cagtgaggcc aaccacctgc aagacggttt ttttttgttg
      901 ttcacaaaat cggggtgaaa gtctgtggat gagatgggtg taagtgggtg gggttgggtg
      961 ttttgggtta ctgaaaatcc cgctcaaaat tcaagcgaat ctcgcgcgcg atccttgtat
     1021 ttatacttaa gggataactc taggattcaa attcgcctcc agcccttgtg ccgcaaggcc
     1081 tgaaggggga tttgtcggaa aatggttttg gagttattct gtcgggagtg gtcgcattaa
     1141 aagcgacccc cgcaacgagg gcaatcgttc gggggtgtga ccacctgtag gaattcgaat
     1201 tggtgatacg actaacacaa caggtgatat ggcaatgata acacgtcaga tgagccatcg
     1261 cccactatcg ggtggtgcgc atgaaaaacc ctgatctcca gaacctcacg gactattccc
     1321 cctcagatgc gccctgggac gctcacagaa gcgtttctga tgacgtggga gggatttacc
     1381 tgcttgcagc cgaatatgag cgctacgggg cgcgtatggc cctctgtggt ggtttactgc
     1441 gtttcgggtg gtctaccctc aaggaaaccg gagaaacgcg tctgaggctg cgtgaggcgc
     1501 atttctgccg tgtccggcat tgtcctgtct gccagtggag gcgctccctg atgtggcaag
     1561 cccgtttcta ccagtccctg ccgcgtatcg tggcggatta tcccgatgcc cgctggatgt
     1621 tcctgactct gaccgtgcgc aactgtgcga tcggggagct gggcgagatg ctgaaccgga
     1681 tgaatgcagc ttttcagcgc atgaaggttc gcaaggagtt ccggcctgtg cagggctgga
     1741 tacgtaccac ggaagtcacc cgcgggagcg acggcagcgc acacccgcac ttccacaccc
     1801 tgatgatggt gccgcctggc atgctaaatg ggaaaagtta cgtcaggcat gaacgctggg
     1861 ttgaactctg gcgggagtgt ctgcgggtcg attatgaccc gaatgttgat gtccgggcag
     1921 tgaaacctcg taagccgaag ggcggcgaat cgctggcctg cgcgaccgcc gaactagtgc
     1981 gcggtgcggt ggccgaaacc ctgaaatatt ccacgaagcc ggctgacatg gtggccgatc
     2041 ctgaatggtt cctcgaactg acccgccaga cgcacaagcg ccgctttgtg gcgaccg
//
