LOCUS       NZ_CP081883             3005 bp    DNA     linear   CON 09-OCT-2023
DEFINITION  Escherichia coli strain WW2 plasmid unnamed6.
ACCESSION   NZ_CP081883
VERSION     NZ_CP081883.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN20855217
            Assembly: GCF_019802685.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3005)
  AUTHORS   Wan,C., Liu,X., Tang,R., Fu,L., Wang,L., Wang,P., Yu,Y. and Wu,C.
  TITLE     Nano-TiO2 mediated antibiotic resistance gene transformation in
            activated sludge: Phenomenon, mechanism and potential risk
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3005)
  AUTHORS   Wan,C., Liu,X., Tang,R., Fu,L., Wang,L., Wang,P., Yu,Y. and Wu,C.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-AUG-2021) Department of Environmental Science and
            Engineering, Fudan University, No. 220 Handan Road, Yangpu District,
            Shanghai, Shanghai 200438, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 700.0x
            Sequencing Technology             :: Illumina; PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 10/09/2023 02:48:27
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,068
            CDSs (total)                      :: 4,953
            Genes (coding)                    :: 4,805
            CDSs (with protein)               :: 4,805
            Genes (RNA)                       :: 115
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 52 of 148
            Pseudo Genes (incomplete)         :: 103 of 148
            Pseudo Genes (internal stop)      :: 26 of 148
            Pseudo Genes (multiple problems)  :: 29 of 148
            Pseudo Genes (short protein)      :: 4 of 148
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP081883.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..3005
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="WW2"
                     /isolation_source="sludge from the aeration tanks of urban
                     wastewater treatment plants"
                     /db_xref="taxon:562"
                     /plasmid="unnamed6"
                     /geo_loc_name="China:shanghai"
                     /collection_date="2020-10-04"
     gene            <1..256
                     /gene="mobA"
                     /locus_tag="K5O11_RS23450"
                     /old_locus_tag="K5O11_23445"
     CDS             <1..256
                     /gene="mobA"
                     /locus_tag="K5O11_RS23450"
                     /old_locus_tag="K5O11_23445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052604.1"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="plasmid mobilization protein MobA"
                     /protein_id="WP_219370660.1"
                     /translation="EDQEIRKRAAECGKTVSGFLRAAALGKKVNSLTDDRVLKEVMRLG
                     ALQKKLFIDGKRVGDREYAEVLIAITEYHRALLSRLMAD"
                     /name="mobA"
     gene            complement(565..948)
                     /locus_tag="K5O11_RS23455"
                     /old_locus_tag="K5O11_23450"
     CDS             complement(565..948)
                     /locus_tag="K5O11_RS23455"
                     /old_locus_tag="K5O11_23450"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_022631129.1"
                     /translation="MKFFKPKTDAIEYIRVEPSFSKTSSNINNAKSGSDTNSQPGFILR
                     SQIEEMIKNHRPKTEKEIKAESEGLIRLCSMAFIFLIVLSIFMLIVYFGIKDRELPDFA
                     NNLMALMTTLVGAIGGFIYGTKK"
                     /name="hypothetical protein"
     gene            complement(945..1622)
                     /locus_tag="K5O11_RS23460"
                     /old_locus_tag="K5O11_23455"
     CDS             complement(945..1622)
                     /locus_tag="K5O11_RS23460"
                     /old_locus_tag="K5O11_23455"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_023233475.1"
                     /translation="MENKAVTISTQLAIFIDSTIARPDLLFNSLNSEIGEVIDAMPQTL
                     PLPADAPADIPRVIGTSSFGKFNLNISLNRIDFVENYTPTEEIEKSVTEFKLKCNTLIA
                     TIFQKYGVVRIGLVGNYYIPHKNPSQLIADLYLNENKRNLDEITIRINNRDTFKNIQIN
                     NVINLSQGQVTAQNYNGNAVIVQLDYNSTDNNTPLDEGLTLALFKEKSIAYNSKHVEDV
                     SGL"
                     /name="hypothetical protein"
     gene            complement(1823..1984)
                     /locus_tag="K5O11_RS25490"
     CDS             complement(1823..1984)
                     /locus_tag="K5O11_RS25490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001354639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071784682.1"
                     /translation="MHEPPVQPDHCALSGNYRLESNPVRHAKAPLAAAIGNRVSRARFV
                     QMLTEFLK"
                     /name="replication initiation protein"
     gene            2188..2421
                     /locus_tag="K5O11_RS23465"
                     /old_locus_tag="K5O11_23460"
     CDS             2188..2421
                     /locus_tag="K5O11_RS23465"
                     /old_locus_tag="K5O11_23460"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_015675076.1"
                     /translation="MSVDFCDARQGGGAYGKTPAVLASSGALLFAHMFFPALSPDSVDN
                     RITAFECADTARRSRTTERSESVSEEAEERLM"
                     /name="hypothetical protein"
     gene            complement(2471..2665)
                     /locus_tag="K5O11_RS23470"
                     /old_locus_tag="K5O11_23465"
     CDS             complement(2471..2665)
                     /locus_tag="K5O11_RS23470"
                     /old_locus_tag="K5O11_23465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_001041644.1"
                     /translation="MNKQQQTVLNMAGFIKSQSLTLLEKLDALDADEQATMCEKLHELA
                     EELQNSIQTRFEVENSTEI"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            2938..>3005
                     /locus_tag="K5O11_RS23475"
                     /old_locus_tag="K5O11_23470"
                     /pseudo=""
     CDS             2938..>3005
                     /locus_tag="K5O11_RS23475"
                     /old_locus_tag="K5O11_23470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052604.1"
                     /note="incomplete; too short partial abutting assembly gap;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="nikA protein"
                     /name="nikA protein"
     Typing          2703..2929
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="91.25"
                     /coverage="99.0"
                     /evalue="2.24e-83"
     Typing          complement(1913..2442)
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.6"
                     /coverage="97.0"
                     /evalue="0.0"
ORIGIN
        1 agaagaccag gaaatcagaa aaagggctgc tgaatgcggc aagaccgttt ccggtttttt
       61 acgggcggca gctctcggta agaaagttaa ctcactgact gatgatcggg tactgaaaga
      121 agttatgaga ctgggggcgt tacagaaaaa actctttatc gacggcaagc gtgtcgggga
      181 tagggaatat gcggaggtgc tgatcgctat tacggagtat caccgtgccc tgttatccag
      241 gcttatggca gattagcttc ccggggagaa aactgtcgaa aacagacggt atgaacaccg
      301 taagccccca aaccgatcgc cattcacttt catgcatagc tatgcagtga gctgaaagca
      361 atcctgacgc atttttccgg tttaccccgg ggaaaacatc tctttttgcg gtgtctgcgt
      421 cagaatcgcg ttcagcgcgt tttggcggtg cgcgtaatga gatgttatgg taaatggtgt
      481 caggtaacgg ttgtaaaagc cccgaagtat ggggctggtg tgcttgtgtg ttatttgttt
      541 tttttatttg tgtttttgtc tgtgttattt ttttgttcca tatataaaac cacctatcgc
      601 tccaactaat gttgtcatta gtgccatcag gttatttgca aaatctggta gttctctatc
      661 ttttattccg aaataaacga ttaacataaa aattgataaa actattaaaa atataaaggc
      721 catggagcaa agccttataa gaccttcgct ttcagccttt atttcttttt ctgttttcgg
      781 tctgtggttt tttatcattt cctctatttg agatcttaaa atgaatccag gctgtgagtt
      841 tgtgtcactt cctgatttcg cattatttat attgctactt gtttttgaga atgatggttc
      901 tacacgaatg tattcaatcg catctgtttt tggtttgaaa aatttcataa tccactcaca
      961 tcctcaacat gcttggagtt ataagcaatg gatttttctt taaatagggc taaggttaac
     1021 ccttcgtcaa gtggtgtgtt attatcggtt gagttgtagt caagttgaac tatcaccgca
     1081 tttccattgt agttctgtgc ggttacttgt ccttggctta aattgatgac attgtttatt
     1141 tgtatatttt tgaatgtatc gcggttattt attcttattg ttatttcatc taaatttctt
     1201 ttgttttcgt ttaaatacag atcagctatt agttgagatg gatttttatg tgggatgtaa
     1261 tagttaccaa ctaggccgat tcttacgact ccatattttt ggaatattgt tgctattagc
     1321 gtattgcact ttaatttgaa ttccgttact gatttttcga tctcctcagt aggggtgtag
     1381 ttttctacaa aatctattct gtttagtgat atgtttaagt tgaacttgcc aaaggacgat
     1441 gtgccaataa cgcgagggat atctgcaggt gcatctgcag gcaggggaag cgtctgtggc
     1501 attgcgtcta taacttcgcc aatttctgag tttaggctat taaagagaag atctggtcta
     1561 gctatggtgc tgtcaataaa tattgctagc tgtgttgata ttgtgacggc cttgttttcc
     1621 attttttctt tcctattttg cattggtatt aattgaagta ttgcgaaatt aacgtgttgt
     1681 gagtatctta aacgaaaaaa ccaccctggc aggtggtttt tcgaaggtta gctaatcctg
     1741 gcagattgtc taaccgggta actggcttca gcagagcgca gataccaaat actgtccttc
     1801 tagtgtagcc gtagtcaggc catcacttca agaactctgt aagcatctgg acaaatctcg
     1861 ctctgctaac tcggttacca atggctgctg ccagtggtgc ttttgcatgt cttaccgggt
     1921 tggactcaag acgatagtta ccggataagg cgcagtggtc gggctgaacg gggggttcgt
     1981 gcacacagcc cagcttggag cgaacgacct acaccgggcc gagataccta cagcgtgagc
     2041 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
     2101 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
     2161 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
     2221 ggcggagcct atggaaaaac gcctgcggtg ctggcttctt ccggtgcttt gctttttgct
     2281 cacatgttct ttccggcttt atcccctgat tctgtggata accgtattac cgcctttgag
     2341 tgcgctgaca ccgctcgccg cagtcgaacg accgagcgta gcgagtcagt gagcgaggaa
     2401 gcggaagagc gccttatgtg acattttctc cttacgctct gttgtgccgt tcggcatcct
     2461 gccctgagcg ttatatctct gtgctatttt ctacttcaaa gcgtgtctgt atgctgttct
     2521 ggagttcttc tgcgagttcg tgcagcttct cacacatggt ggcctgctcg tcagcatcga
     2581 gtgcgtccag tttttcgagc agcgtcaggc tctggctttt tatgaatccc gccatgttga
     2641 gtacagtttg ctgctgcttg ttcatctttc tgttttctcc gttctgtctg tcatctgcgt
     2701 cgtgtgatta tatcgcgcac cacttttgga ccgtcttacc gccggtattc tgccgacgga
     2761 catttcagtc agacaacact gtcactgcca aaaaactgcc gtgcctttgt cggtaattcg
     2821 agcttgctga caggacagga tgtgtaattg ttataccgcg catacatgca cgctattaca
     2881 attaccctgg tcagggcttt gccccgacac cccatgtcag atacggagcc atgttttatg
     2941 acaaaacgaa gtggaagtaa tacgcgcagg cgggctatca gtcgccctgt tcgtctgacg
     3001 gcaga
//
