LOCUS       NZ_CP081882             1551 bp    DNA     linear   CON 09-OCT-2023
DEFINITION  Escherichia coli strain WW2 plasmid unnamed8.
ACCESSION   NZ_CP081882
VERSION     NZ_CP081882.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN20855217
            Assembly: GCF_019802685.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1551)
  AUTHORS   Wan,C., Liu,X., Tang,R., Fu,L., Wang,L., Wang,P., Yu,Y. and Wu,C.
  TITLE     Nano-TiO2 mediated antibiotic resistance gene transformation in
            activated sludge: Phenomenon, mechanism and potential risk
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1551)
  AUTHORS   Wan,C., Liu,X., Tang,R., Fu,L., Wang,L., Wang,P., Yu,Y. and Wu,C.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-AUG-2021) Department of Environmental Science and
            Engineering, Fudan University, No. 220 Handan Road, Yangpu District,
            Shanghai, Shanghai 200438, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 700.0x
            Sequencing Technology             :: Illumina; PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 10/09/2023 02:48:27
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,068
            CDSs (total)                      :: 4,953
            Genes (coding)                    :: 4,805
            CDSs (with protein)               :: 4,805
            Genes (RNA)                       :: 115
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 52 of 148
            Pseudo Genes (incomplete)         :: 103 of 148
            Pseudo Genes (internal stop)      :: 26 of 148
            Pseudo Genes (multiple problems)  :: 29 of 148
            Pseudo Genes (short protein)      :: 4 of 148
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP081882.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..1551
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="WW2"
                     /isolation_source="sludge from the aeration tanks of urban
                     wastewater treatment plants"
                     /db_xref="taxon:562"
                     /plasmid="unnamed8"
                     /geo_loc_name="China:shanghai"
                     /collection_date="2020-10-04"
     gene            714..1190
                     /locus_tag="K5O11_RS23445"
                     /old_locus_tag="K5O11_23440"
     CDS             714..1190
                     /locus_tag="K5O11_RS23445"
                     /old_locus_tag="K5O11_23440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000469298.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_000469298.1"
                     /translation="MFIDSEKRLKQLSDEAKKNTEDLEEAKKNSRFTQVSPKGWERVRE
                     LLKDSQGISALKLYSFLAEHIDPTCGAVVADQQFLAEKLGVSRSTIIRWLNYLESKNAL
                     VRIPVAGKVCAYALDPHEVWKGYNTTKNHAAFVTKTLVNKDGDIQRRIMAMFSN"
                     /name="helix-turn-helix domain-containing protein"
     Typing          244..324
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="95.122"
                     /coverage="87.0"
                     /evalue="9.59e-28"
     Typing          complement(527..635)
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="1.95e-50"
ORIGIN
        1 ccttccatgc cctgacgggc atttaaaacc cgtttcgtgt gtctgagagc ttctgagcgc
       61 gtttgagggg ctatctggcg actcatcccc gaaagaacaa caacggaatc accgacaaca
      121 tgcgagaggt gacaccgctc gccgcagccc cagcgaccga acgtagtgag cgagtgggcg
      181 aggaagcgga agagaaccgg aaaccacatt gagcacgtac gcactgatgc ggggtgtcgg
      241 ggcgaagccc tgaccagagg gcaaacggaa tatcgtcgcg ggtgacggta ttacatttgc
      301 acatcctgtc ccgatttctg aggcgtttta gcggtgaacg gacggcaaaa ccatgatgga
      361 gctgttgagc gttgagagcg tgaaatctgg caaaaatcac ggctggcgag gcttgtagcg
      421 gaataagcca gtatacacat cgctatcgct acgtgactgg gtcagggctt cgccccgaca
      481 ccccaaaagg gcgttgcgtt gcgcgcaaca cccttgccta gaatagatct tttacgccga
      541 tttgtaagtt gttgtttttt tagtgtgttt tttgtttacc tgtcgcatcc agggtgacac
      601 aacgttgtcg catcctggat gcgacaacac gcaacaaggt gttgcacaga tgtgtcatga
      661 tggcttagat ttgaaccaac gttagtgcat gggattttca gagggaaaaa atcatgttta
      721 ttgattcaga aaaacgactg aaacaacttt cagatgaggc aaagaaaaac accgaggatc
      781 tcgaagaagc aaagaagaat tcaaggttta cacaggtatc cccaaaaggt tgggaacgcg
      841 ttcgagagct gctgaaggat agtcaaggca tatcagcact gaagctgtac tcatttttag
      901 cggagcatat cgatcctacg tgtggcgctg tcgttgcgga tcagcaattc ctagctgaaa
      961 aacttggagt tagcagaagc acaattattc ggtggctcaa ttacttagaa tcaaaaaatg
     1021 cattagttag aatccccgtt gctggtaagg tttgtgcgta tgccctcgat ccacatgaag
     1081 tctggaaggg atacaacact acgaaaaacc atgcagcgtt tgtcactaaa acactggtca
     1141 acaaagacgg tgatattcag cgccgaatca tggccatgtt ttcaaattga gccagcggca
     1201 ggcggacaat caggggctac gtgttaacgt tttgaccatg attgtctatc ctgcattgct
     1261 cttttgccgc ctcaaaatcc cttgcttgtt tttgctcccc gatccccaga acgaccgcgc
     1321 cgccacggtt ccggcagcgc cttgagcgca accggaaacc gtctggggag aaccccagac
     1381 ccccatcgct gtaacaccga cggtctacga ccaccaacca gccgaatagc tgcctgtgcc
     1441 gtgcaaatag gctttgcagg gggctgacgc ggttcctagc gacctgaaag cccctggtta
     1501 ggggtatcgg cgcgcctgac ctcctgagag cctctgtaag cgtttttcgc g
//
