LOCUS       NZ_CP081824             5596 bp    DNA     circular CON 10-JAN-2024
DEFINITION  Klebsiella pneumoniae strain F726925 plasmid pF726925-4, complete
            sequence.
ACCESSION   NZ_CP081824
VERSION     NZ_CP081824.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN20855505
            Assembly: GCF_019793595.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 5596)
  AUTHORS   Zhou,D.
  TITLE     Complete sequences of strain F726925
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5596)
  AUTHORS   Zhou,D.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-AUG-2021) State Key Laboratory of Pathogen and
            Biosecurity, Beijing Institute of Microbiology and Epidemiology,
            No.20, Dongdajie, Fengtai, Beijing, Beijing 100071, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP v. v3.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100.0x
            Sequencing Technology             :: PacBio RSII
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/10/2024 03:27:42
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,754
            CDSs (total)                      :: 5,632
            Genes (coding)                    :: 5,489
            CDSs (with protein)               :: 5,489
            Genes (RNA)                       :: 122
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 143
            CDSs (without protein)            :: 143
            Pseudo Genes (ambiguous residues) :: 0 of 143
            Pseudo Genes (frameshifted)       :: 58 of 143
            Pseudo Genes (incomplete)         :: 85 of 143
            Pseudo Genes (internal stop)      :: 16 of 143
            Pseudo Genes (multiple problems)  :: 16 of 143
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP081824.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5596
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="F726925"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pF726925-4"
                     /geo_loc_name="China: Jiangsu"
                     /collection_date="2015"
     gene            join(5508..5596,1..262)
                     /locus_tag="K5O13_RS28545"
                     /old_locus_tag="K5O13_28545"
     CDS             join(5508..5596,1..262)
                     /locus_tag="K5O13_RS28545"
                     /old_locus_tag="K5O13_28545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004197756.1"
                     /translation="MYWLIAFLAFIFPGRSFADGYTAYYRCNGTQEASLTVKDNKLSMV
                     VGLSKFNRQLDTYRFKEKGIEVVMQRAASDDNSEAIMSFIMPDVNNMTLLVKNNGYIIV
                     KTTCWLTKTDKQ"
                     /name="hypothetical protein"
     gene            complement(332..598)
                     /locus_tag="K5O13_RS28550"
                     /old_locus_tag="K5O13_28550"
     CDS             complement(332..598)
                     /locus_tag="K5O13_RS28550"
                     /old_locus_tag="K5O13_28550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262326.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_004197762.1"
                     /translation="MVWTINYSDRALKSLRKMDKQNARRIVDFMSLRIAVAADPRQSGK
                     PLKGELGEFWRYRVGDYRVLCEIRDDELVILAATIGHRREVYD"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(582..806)
                     /locus_tag="K5O13_RS28555"
                     /old_locus_tag="K5O13_28555"
     CDS             complement(582..806)
                     /locus_tag="K5O13_RS28555"
                     /old_locus_tag="K5O13_28555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6290 family protein"
                     /protein_id="WP_000879771.1"
                     /translation="MLAIRLSDEIESRLDSLAKQTGRTKTFYAREAILAHLEDLEDYYL
                     SAETAARVRRGDEAVHSSEDVRKSLGLDD"
                     /name="DUF6290 family protein"
     gene            complement(1473..1685)
                     /locus_tag="K5O13_RS28560"
                     /old_locus_tag="K5O13_28560"
     CDS             complement(1473..1685)
                     /locus_tag="K5O13_RS28560"
                     /old_locus_tag="K5O13_28560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_021527181.1"
                     /translation="MTELEKQLLSALEQLQQDYSKRLDEWESAFGEWRKMSGLMQRENA
                     ALSERVTRLSQQVERLSGQLQRLSR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(1692..2201)
                     /locus_tag="K5O13_RS28565"
                     /old_locus_tag="K5O13_28565"
     CDS             complement(1692..2201)
                     /locus_tag="K5O13_RS28565"
                     /old_locus_tag="K5O13_28565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_032433972.1"
                     /translation="MNSLLTLAKDLEQKSKAQQQSTGEMLKAAFSEHEKSVRAELSESG
                     KRISAAIRAHEQGMSAAMQSNRLSVMRMVGRTWLTITMVSGLLFASLSGVLWYQGSLIA
                     SNLAEIDRQNAALSKLNAKTWGVTYLEDSNGRFLVLPKGTAVDRTQSWTVGNGRSKQNA
                     LRLVKE"
                     /name="MbeB family mobilization protein"
     gene            complement(<2236..2940)
                     /locus_tag="K5O13_RS29020"
                     /old_locus_tag="K5O13_28570"
                     /pseudo=""
     CDS             complement(<2236..2940)
                     /locus_tag="K5O13_RS29020"
                     /old_locus_tag="K5O13_28570"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(2879..3223)
                     /locus_tag="K5O13_RS28575"
                     /old_locus_tag="K5O13_28575"
     CDS             complement(2879..3223)
                     /locus_tag="K5O13_RS28575"
                     /old_locus_tag="K5O13_28575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006122514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_062955149.1"
                     /translation="MAVKRTRFLGIRVTDGEYQQLLERCNGRQLAVWMRETCLDTRPAR
                     SLRLPSIDPVLLRQLAGMGNNLNQIARKINGGQWSGADRVQVVAALMAIDAELERLRHA
                     VLEKGADDDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            complement(3213..3389)
                     /locus_tag="K5O13_RS28580"
                     /old_locus_tag="K5O13_28580"
     CDS             complement(3213..3389)
                     /locus_tag="K5O13_RS28580"
                     /old_locus_tag="K5O13_28580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004197757.1"
                     /translation="MQKPKKKPLVVKKRSWHPAVMANLMRQAQEEDYRREQMAELERSA
                     RSHFADIGGEDGG"
                     /name="hypothetical protein"
     gene            4029..4187
                     /locus_tag="K5O13_RS29025"
     CDS             4029..4187
                     /locus_tag="K5O13_RS29025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071596352.1"
                     /translation="MHEPPVQPDCCALSGNYHLESNPDGHAKTPLAAAIGNWIYTREIL
                     RGLEVGA"
                     /name="replication initiation protein"
     gene            4545..4871
                     /locus_tag="K5O13_RS28585"
                     /old_locus_tag="K5O13_28585"
     CDS             4545..4871
                     /locus_tag="K5O13_RS28585"
                     /old_locus_tag="K5O13_28585"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_050516389.1"
                     /translation="MRERTNIMLDIGLKQSLQRLSNKTKMSLSDLINQRLSGSLSVEER
                     IGEKPEWMRNLDPYLANHFSNIELPLPSPLKEYQEKMDEIDSIINDMMVLKEYFRAKFY
                     DEIK"
                     /name="hypothetical protein"
     gene            4897..5442
                     /locus_tag="K5O13_RS28590"
                     /old_locus_tag="K5O13_28590"
     CDS             4897..5442
                     /locus_tag="K5O13_RS28590"
                     /old_locus_tag="K5O13_28590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_023317533.1"
                     /translation="MFFKRYLTEKEIYKAALPIVRLQGRVNTSLRFLSKYSDVNKNGLI
                     ALHLNLLHFISYNIHGVESLKITQAGINKAVQQSPHADEIKNIAQAIGRKGPEYIEGLI
                     RNPWGKKNDNPLIFIMSIEESIKETLNSGYMLYRVAPITEGEAAAFMDVLKQLLLFEDN
                     FRLFGRDLVYKIEKLKIR"
                     /name="hypothetical protein"
     Typing          complement(3476..3552)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="91.25"
                     /coverage="98.0"
                     /evalue="1.04e-21"
     Typing          3741..4138
                     /name="rep_cluster_2370"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="89.526"
                     /coverage="91.0"
                     /evalue="3.03e-139"
     Typing          complement(1358..2889)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.804"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 tcaggaagca tctcttactg ttaaagataa caaactaagt atggttgtag gtctttctaa
       61 gtttaatagg caactggata cttatagatt caaagaaaag ggaatcgaag tagtaatgca
      121 gagagctgct tctgatgata actctgaggc cataatgtct tttattatgc ctgacgttaa
      181 caatatgacc ttattagtta aaaataacgg ctacattatc gttaagacaa cctgctggtt
      241 aactaaaacc gacaaacagt agaacacgta gttagtcgag tatttaccat aatacctgtt
      301 gtcggcatca aaaaagcccg catagcgggc ttcagtcgta aacttcgcgg cgatgtccaa
      361 tcgtggcggc aaggataaca agctcgtcat ctcggatctc acacagaacg cgataatctc
      421 ccacccgata gcgccagaac tcgcccagct cacctttgag cggcttccct gactggcgag
      481 gatcggcagc aactgcaatg cgtaaactca taaaatccac aatccgtcgt gcgttctgtt
      541 tgtccatctt gcgtaacgat ttgagcgccc gatcggaata gttaatcgtc cagaccaagt
      601 gacttcctca cgtcttcaga cgaatgcact gcttcatcac cacggcgaac gcgtgcagca
      661 gtttctgctg aaagataata atcctccagg tcttccagat gcgccagtat tgcttcccgc
      721 gcataaaacg tctttgttct gccggtttgc ttcgccagcg agtccagacg ggactcaatc
      781 tcatcagata accggatagc aagcatataa acctcccgaa caccatttgc tatacatgta
      841 tatcaacagt accacaatca acctaaaaga acaaaaaata atcttgttag cccctcttga
      901 taatccgtaa cgcggtcaat atatctagct tgagggcttt cctcttaact attgaaagga
      961 taacgattat gggtcttaag ccgggtccga aacgaattgc cgattcaaca ggggaacctg
     1021 ataaacgcca gcgtgacaat aaaaaaacgc cgggaaatac tgacaaattg aaacccagca
     1081 aatcttcaaa aaaataagtc agtagttaaa agccctccgg ggcttttttg tatctgccgt
     1141 tcacgctgat taaacataac acccgttgcc cgcaccgccc cggacgggtt cgccgcgaac
     1201 ggggtggcag tcaggtaaaa ccggcaacaa accacccgat aaaccgcccc gttcgcgaca
     1261 gataaatttt acctggctgc atagccatgc agccaggtga atgacatccg gtttcagcgt
     1321 aaatgcccga tgagaaccct gttttcctgc atttcagccc agcgacggac cgtcatagct
     1381 tcgctgccgg gaacgcgctt ccgtcagttc cttttcgtgc tgccgttgct cgtgcgcccg
     1441 ttctgcggcc acggccatct catgacgctg cactaccggc tcaaacgctg cagttgcccg
     1501 ctcaagcgtt ccacctgctg actcaagcgc gtcacgcgct cgctcagcgc cgcgttctcc
     1561 cgttgcataa gccctgacat tttccgccat tccccgaagg cgctctccca ctcgtccagc
     1621 cttttcgagt agtcctgctg tagctgctct aatgcgctca gcaactgttt ttccagctcg
     1681 gtcatcggca cttactcctt caccagtctc agcgcgttct gcttcgaccg cccgttcccg
     1741 accgtccagc tctgcgtcct gtctacggcc gtccccttcg gcagcaccag aaaacgtccg
     1801 ttgctgtctt ccaggtacgt cacgccccag gtcttcgcgt tcagcttcga cagcgctgcg
     1861 ttctgccggt cgatttcggc cagatttgac gctatcaagc tcccctgata ccacaacacc
     1921 ccgctcaggc tggcgaacag cagtcctgac accatcgtga tggtcagcca tgtccgcccg
     1981 accatacgca tcacgctcag gcggttcgac tgcatggccg cgctcatacc ctgctcgtgg
     2041 gcgcggatgg cggcgctgat tctcttcccg ctttcgctca gttccgctct gacagacttc
     2101 tcgtgctcgc tgaatgcggc tttcagcatt tcgccggtac tctgctgctg cgctttcgat
     2161 ttctgctcta agtccttcgc cagcgttaaa agactgttca tagatggctc ccttaagtcg
     2221 gatgtttcgc cctccgtccg ggtcggcaat gctgatgctg ctctttgtgg tgcgcacgac
     2281 ctcaaaacct gcgctttcca gcgcctcagt gacgtcctga cgcgttttaa gctcccctga
     2341 cgaggcgagg gccagtaagc cccgcgtaat agcctctgcg gcttcctgct tcgttttggg
     2401 taacgaagat ggcgtcacca gcgcgcgccg gttctccggt gcgttcgggt cgtgcagccc
     2461 cagtcgcccg ttcaccacgg tctgccacgc atcgatgcgc ggacggtctg cccggtcgta
     2521 gtacggctgg agacgcctgc cggtcagcag ttccgtgttc ggtaccagaa aattcagttc
     2581 cagccgcccc ttgtcccggt gttcaaccca cagcacgctg tactggtctt tatcgagtcc
     2641 gggcatcaga acccgctcga agctcgccat cagcttttca cgctggccgg gcggtaaatc
     2701 ctgttcagca aaagacagga cgccggaggt gtactttttg gcgtagggcg aggcgtcgat
     2761 aagctcccgg acctcctccg gctttccctg caggacgatg gccccgtcgc gctggcggtc
     2821 tttccccagc agataatcca ccggcccggc accgccgcct cgccctcgcg gatgaaactt
     2881 aacgatcatc gtctgccccc ttttccagca cggcatgccg cagccgctcg agttcggcat
     2941 caatggccat cagcgcggcc accacctgaa cccggtcagc gcctgaccac tggccgccgt
     3001 taattttccg ggcgatctgg ttgaggttgt tccccatccc ggcaagctgg cgcagcaaaa
     3061 ccggatcgat ggacggcagc cgcaacgacc gcgccgggcg cgtatcgaga caggtttccc
     3121 gcatccacac cgccagttgc cttccattgc agcgctccag cagctgctgg tactccccat
     3181 cggtcacccg gatacctaaa aaacgggtac gcttaaccgc catcctctcc tccgatatcc
     3241 gcaaaatgtg accgggctga acgctcaagc tctgccatct gctcccggcg gtaatcttcc
     3301 tcctgtgcct gacgcatcag atttgccatc actgccggat gccaggatct tttttttacg
     3361 acgagcggct tcttcttcgg cttctgcatt atccctcctt cgcaaacgcc ggacggcacg
     3421 gaaaagtttt ccgggtcgct ccggcgttgg acaagcgcca gcgcgtcagt ggtgttagcg
     3481 ggtttcgggg cgcagccctg aaccagtcac agcgtttacg ctgtgtatac tggcttacta
     3541 tgttggcatg agagcacgat tcatgaaagt gcctcatgtg gcaggtgtga tatgtggctc
     3601 aggagcgtcc gcaataccat gcacggacag atgaaatgac gcctcctcgc tcactcggta
     3661 gctgcgctcc tgccgttcgg ctgcggcggg cgtgaccgac tcacggataa cgggataaag
     3721 caaaggcgct caaatcagaa agggccggag aaaaggatgc ggcgttgccg tttttccata
     3781 ggctccgcct cccctgacga gcatcacaga aatggacgct caagtcagag gaggacgaaa
     3841 cccgacagga cttaaagatg ccggtgattt ctctccggcg gctccctcgt gcgctctcct
     3901 gttccgaccc tgccgtttac cggatacctg ttccgccttt ttccctttcg ggaagcgtgg
     3961 cgctttctca tagctcacgc tgctggtatc tcggctcggt ttaggtcgtt cgctccaagc
     4021 tgggctgtat gcacgaaccc cccgttcagc ccgactgctg cgccctttcc ggtaactatc
     4081 atcttgagtc caacccggac ggacacgcaa aaacgccact ggcagcagcc attggtaact
     4141 ggatatatac gagagagata ttgagaggtc ttgaagtggg tgcctaactg cggctacact
     4201 gaaagaacag ttttggtatc tgcggtctcg tatacccagt tacccggtta agcagttccc
     4261 caactgactt aaccttcgat caaaccgcct ccccaggcgg ttttttcgtt tacagtgcaa
     4321 gagattacgc gcagaaaaaa aggatctcaa gaagatcctt taaatgatcg tcagttctgt
     4381 tatccccgga gctccatcca tcccacggaa aaacacccgc cactgatgcc ctcagcgcac
     4441 tgcacatcat gtacaaacca ctccctccct ccttttttat tgcgtttctt aatgcgcaat
     4501 ggtagctttg tgcgcaacaa aaaacgcaat agcaaggggg ggtaatgaga gaaagaacaa
     4561 atatcatgct agatatagga ttaaagcaat ccttacaacg gctttcgaat aaaaccaaaa
     4621 tgagtctaag tgatcttatt aaccagagat tatcaggttc actttcagtt gaagaacgca
     4681 ttggcgaaaa gcctgaatgg atgagaaacc ttgatcccta tctcgccaat cacttttcaa
     4741 atattgagtt accgctccct tcaccattaa aagaatacca agaaaaaatg gatgagatag
     4801 attcaattat taatgacatg atggttctaa aggaatattt cagagctaag ttttacgacg
     4861 aaataaaata ggcacgctat acggaggcgg agaattatgt tttttaaacg gtatttaacg
     4921 gagaaggaaa tatacaaggc agcactccca attgttaggc ttcaagggag agttaacact
     4981 tcattacgtt ttttatccaa atactcagat gtaaacaaaa atggattaat tgcattgcat
     5041 cttaatttat tacattttat ctcatacaac atacacggtg tagagtctct gaaaatcact
     5101 caagcaggca taaacaaagc tgtacaacaa agccctcacg cagatgaaat aaaaaatatt
     5161 gcgcaggcaa taggacgcaa aggacctgag tacatcgaag gattaattag aaacccatgg
     5221 gggaagaaaa atgataatcc actaatattt ataatgagca ttgaagaaag tataaaagaa
     5281 acccttaact caggttatat gttatataga gtagctccca taactgaggg tgaagcagct
     5341 gctttcatgg atgttcttaa acaattatta ttatttgagg ataattttcg cctttttggt
     5401 agagacttgg tttataaaat agaaaaatta aaaatcagat aacaaagtat ggtgaacgaa
     5461 aaatgaaaga gaggatagtg aaatgtgtaa taccaagatt ctcaactatg tattggttaa
     5521 ttgcgttcct agcatttata ttccccggtc gatcttttgc tgacggttac accgcttact
     5581 accgatgtaa tggtac
//
