LOCUS       NZ_CP081823            10060 bp    DNA     circular CON 10-JAN-2024
DEFINITION  Klebsiella pneumoniae strain F726925 plasmid pF726925-3, complete
            sequence.
ACCESSION   NZ_CP081823
VERSION     NZ_CP081823.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN20855505
            Assembly: GCF_019793595.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 10060)
  AUTHORS   Zhou,D.
  TITLE     Complete sequences of strain F726925
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10060)
  AUTHORS   Zhou,D.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-AUG-2021) State Key Laboratory of Pathogen and
            Biosecurity, Beijing Institute of Microbiology and Epidemiology,
            No.20, Dongdajie, Fengtai, Beijing, Beijing 100071, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP v. v3.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100.0x
            Sequencing Technology             :: PacBio RSII
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/10/2024 03:27:42
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,754
            CDSs (total)                      :: 5,632
            Genes (coding)                    :: 5,489
            CDSs (with protein)               :: 5,489
            Genes (RNA)                       :: 122
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 143
            CDSs (without protein)            :: 143
            Pseudo Genes (ambiguous residues) :: 0 of 143
            Pseudo Genes (frameshifted)       :: 58 of 143
            Pseudo Genes (incomplete)         :: 85 of 143
            Pseudo Genes (internal stop)      :: 16 of 143
            Pseudo Genes (multiple problems)  :: 16 of 143
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP081823.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..10060
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="F726925"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pF726925-3"
                     /geo_loc_name="China: Jiangsu"
                     /collection_date="2015"
     gene            165..410
                     /locus_tag="K5O13_RS28480"
                     /old_locus_tag="K5O13_28480"
     CDS             165..410
                     /locus_tag="K5O13_RS28480"
                     /old_locus_tag="K5O13_28480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032440458.1"
                     /translation="MTHLKLTPAEEKRLADAFVAQALAMGLKGNKTLPKAVKQQVRERV
                     LRAIQDEREARRAQEMRREKYEATETTFTWQPQRRR"
                     /name="hypothetical protein"
     gene            684..1055
                     /gene="tolA"
                     /locus_tag="K5O13_RS28485"
                     /old_locus_tag="K5O13_28485"
     CDS             684..1055
                     /gene="tolA"
                     /locus_tag="K5O13_RS28485"
                     /old_locus_tag="K5O13_28485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237044.1"
                     /GO_component="GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA]"
                     /GO_function="GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="WP_001237044.1"
                     /translation="MRLIPFILLALCSPLLLTACKHSTTQPPKIYTLDKSSKISSYAAA
                     IKTAVEMQMFEPEAYAGKMCTVRISIQRDGTLNSAIAEGGDPKLCKAAISAVTHAKIPP
                     APDENTWQIFKNAPLDFRP"
                     /name="tolA"
     gene            1052..1417
                     /locus_tag="K5O13_RS28860"
     CDS             1052..1417
                     /locus_tag="K5O13_RS28860"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /GO_function="GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="WP_072354022.1"
                     /translation="MKAFTLTVLAAMFLTPVHAIPLKPVTTLQPTYPVQAAAKRIEGQV
                     TATFDILDDGRVDNVSLTGCHLFFRETLTAMRHWLYPAGHPAMRQVINFEFSLKNDGLQ
                     DPRPVTTQKLNDDGCRQ"
                     /name="TonB family protein"
     gene            1592..1870
                     /locus_tag="K5O13_RS28495"
                     /old_locus_tag="K5O13_28495"
     CDS             1592..1870
                     /locus_tag="K5O13_RS28495"
                     /old_locus_tag="K5O13_28495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014542884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family ribbon-helix-helix protein"
                     /protein_id="WP_000093040.1"
                     /translation="MSSATSIRLDEELKDRLKTLADDRHRSAHALMLEAITEYIDREEK
                     RSQYLRDGQAAWQHYQETGLHLTAEEAEAWISTWGTENEQDAPPCHR"
                     /name="CopG family ribbon-helix-helix protein"
     gene            1858..2172
                     /locus_tag="K5O13_RS28500"
                     /old_locus_tag="K5O13_28500"
     CDS             1858..2172
                     /locus_tag="K5O13_RS28500"
                     /old_locus_tag="K5O13_28500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001136729.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]; GO:0009432 - SOS response [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_071177730.1"
                     /translation="MPQVTISALAQRDLQRLQDFLKTKNRLAARKGGEVIVRAIQQLKT
                     LPDIGRPVPFLPLEYKELVIGFGDSGYVMLYRHDREMDQIVIVTVRHQKESGYPGADSL
                     "
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            2336..2764
                     /locus_tag="K5O13_RS28505"
                     /old_locus_tag="K5O13_28505"
     CDS             2336..2764
                     /locus_tag="K5O13_RS28505"
                     /old_locus_tag="K5O13_28505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001140599.1"
                     /translation="MAKTLLNLSQAAQAAGITRRTLYNHVKQGKVTVSRDGKNNPVVDV
                     SELIRVYGNVNIPEKQIPGISHRENTQKNFPQEQLLAMQKELADLRQAVTLMLEDKTSR
                     EEERRQHDDERRKLQAEVDRLTTELTQKKKRFWSGWFS"
                     /name="hypothetical protein"
     gene            2790..2969
                     /locus_tag="K5O13_RS28510"
                     /old_locus_tag="K5O13_28510"
     CDS             2790..2969
                     /locus_tag="K5O13_RS28510"
                     /old_locus_tag="K5O13_28510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165970.1"
                     /translation="MTKQEKTALNMAKFIKDQSLLLLEKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(2996..3526)
                     /locus_tag="K5O13_RS28515"
                     /old_locus_tag="K5O13_28515"
     CDS             complement(2996..3526)
                     /locus_tag="K5O13_RS28515"
                     /old_locus_tag="K5O13_28515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_071177729.1"
                     /translation="MALYMVFIEDVEDKDGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGENPQWERDKPTGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQINAMIEVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(3533..4264)
                     /gene="mobC"
                     /locus_tag="K5O13_RS28520"
                     /old_locus_tag="K5O13_28520"
     CDS             complement(3533..4264)
                     /gene="mobC"
                     /locus_tag="K5O13_RS28520"
                     /old_locus_tag="K5O13_28520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WP_071177728.1"
                     /translation="MTLEQYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRAKYPVDGRQVSVWGLTPHGVAFSFDEDEPLTDVIPFQP
                     SRVSAAQLPHRLAVQSLRLSMEGRGATGWRYLHRMALKGMKVPDALAELDGRTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRRANGIDEIWYICPDRPTLVRVQRAILAVDEIVNPQTGEA
                     RKTVELDRERLFACFKFMTTE"
                     /name="mobC"
     gene            complement(4264..6228)
                     /locus_tag="K5O13_RS28525"
                     /old_locus_tag="K5O13_28525"
     CDS             complement(4264..6228)
                     /locus_tag="K5O13_RS28525"
                     /old_locus_tag="K5O13_28525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_071177727.1"
                     /translation="MFNTDSLAWQWHIPELARQIYPWVSWLYREYYSLAGFFLVIGLFF
                     GLAELWAGRMRFVGLSHKARREYADRYKRDNNRTVPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLV
                     AGVLLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSTEQARKQQAVFLGKDEQGQPVLVPRDTWRKTNIQILGLPGSGKSAMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLV
                     AGFNLSETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     KLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFRRWPHASIMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPADFV
                     KTTVLDNTPIRWFYRAASQESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARLAFSSPLRVERREISLKTFPVLAKTDPLAEY
                     LPPADGGGPKADDDFAGLY"
                     /name="TraM recognition domain-containing protein"
     gene            complement(7712..7861)
                     /locus_tag="K5O13_RS28530"
                     /old_locus_tag="K5O13_28530"
     CDS             complement(7712..7861)
                     /locus_tag="K5O13_RS28530"
                     /old_locus_tag="K5O13_28530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_032440454.1"
                     /translation="MKKVKTIFLFILIVAGFLLAACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     gene            complement(7946..8203)
                     /locus_tag="K5O13_RS28535"
                     /old_locus_tag="K5O13_28535"
     CDS             complement(7946..8203)
                     /locus_tag="K5O13_RS28535"
                     /old_locus_tag="K5O13_28535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /GO_function="GO:0015643 - toxic substance binding
                     [Evidence IEA]"
                     /GO_process="GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E3-like toxin immunity protein"
                     /protein_id="WP_032440455.1"
                     /translation="MGLKLRLTWFDKKTEEFKGEEYSKDFGDDGSVIESLGMPLKDNIN
                     NGEFDLKKEWVPLLQPFFKNQIKPDVDNCYIAFDYRDGNW"
                     /name="colicin E3-like toxin immunity protein"
     gene            complement(8213..9898)
                     /locus_tag="K5O13_RS28540"
                     /old_locus_tag="K5O13_28540"
     CDS             complement(8213..9898)
                     /locus_tag="K5O13_RS28540"
                     /old_locus_tag="K5O13_28540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase domain-containing
                     protein"
                     /protein_id="WP_032440457.1"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSVSGDALSAAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPKMMSK
                     IMTSLPADAVTETPASTLPLDQATVRVRQRVVDVVKDERQHIAVVAGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPSLQVSVPKGVPTAKAPPKGIVAEKGDSRPAGFTAGGNSREAVIR
                     FPKETGQKPVYVSVTDVLTPAQVKQRQEEEKRRQQAWDAAHPEEGLKRDYDKAKAELDA
                     EDKNIATLNSRIASTEKALPGARAAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNEKLKAAQASLNAMNESLSRDKAALTGAMESRKQKEKKAKDAENKLNE
                     EKNKPRKGTKDYGHDYHPVPKTEDIKGLGELKRGDPKTPKQGGGGKRARWYGDKKRKIY
                     EWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPKRNIKKYL"
                     /name="colicin-like bacteriocin tRNase domain-containing
                     protein"
     BGC             3213..10060
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          7031..7609
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.282"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(3537..4264)
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.416"
                     /coverage="100.0"
                     /evalue="1.34e-140"
ORIGIN
        1 atcgcgtctt ttcccgaaaa tttctcatgg caacaaattt tattatttaa gacattcatc
       61 acacttacat ttcagatggt atagacacat ctgaaagcta actggcatga ggagaggacc
      121 tgcggttaaa ctgttcggga aacctgagac gcccggaggc cactatgacg cacctgaaac
      181 tgactcccgc tgaagaaaag cgcctggcgg acgcctttgt cgctcaggca ctggccatgg
      241 gcctgaaagg gaacaaaacg ctgccaaagg cggtaaaaca acaggtgcgg gaaagggtgc
      301 ttcgtgccat tcaggacgaa cgtgaagcca ggcgggcgca ggaaatgcgt cgggagaaat
      361 acgaggctac ggaaaccacc ttcacctggc agccacagcg ccgccggtaa ggggtattta
      421 ccataacgtc gcattgcccg caccgctcag acggtctgag ggcgtttccg gcggctacgg
      481 gataaaacag gcaacaaacc acccggaaaa ccgccggaaa cgcgcccggt aaattgtaac
      541 ccgctgcatg cctatgcacg caactgcatg gcgggccgtt tggtgtaaaa acgatacgat
      601 aagccgtgtt tatgtaaatg aacgtggccg taaagagtga caaatataca tgtaacattt
      661 tttactgaca ggtcatcaac ccgatgcgtt taataccctt tatcctgctg gcattgtgca
      721 gccccttact cctgacggcc tgtaagcatt ccaccaccca gccgccgaaa atttatactc
      781 tggataagtc atctaaaatc agtagctacg ctgcggccat caagaccgct gttgaaatgc
      841 agatgtttga accagaggcc tatgcgggaa aaatgtgcac cgtgcggata tccatacaac
      901 gtgatggcac tctgaacagc gccattgctg aaggaggaga tccgaaactc tgtaaagcag
      961 caatatcagc cgttacccac gccaaaattc cgccggcacc ggatgaaaat acctggcaaa
     1021 tattcaaaaa cgcccctctg gatttcagac catgaaagct tttactctga cagtgctggc
     1081 ggccatgttc ctcactccgg tacatgcgat accactaaaa cccgtaacca cactgcagcc
     1141 aacctacccg gtacaggccg cagcaaaacg catcgaaggt caggtgaccg ccacgtttga
     1201 tattctggac gatggacgag tggacaacgt gtcgcttacc ggctgtcacc ttttttttcg
     1261 ggaaacgctg acggcaatga ggcactggct atatcccgca gggcacccgg caatgcgaca
     1321 ggttataaat tttgaattca gtctgaaaaa tgacggctta caggatccgc gaccagtcac
     1381 cacccaaaag ctcaatgatg atggttgccg ccagtaacag gtcctttaac ccgcaggctg
     1441 caatagtcct ggatgatgag tgagcctgta gcagccctga ttagtggaag cgaagctgaa
     1501 aaatgggcag caaaagccgt atttctggca aatagcgccg ccacggatta aagtagtcat
     1561 gtgtaataca tatgactaca aaggggcttt tatgtcatca gcaacatcca tcaggctcga
     1621 tgaagaactt aaggaccggc tgaaaacgct ggccgatgac cggcaccgct cagcgcacgc
     1681 gctaatgctg gaggcaatca ccgaatacat cgaccgggaa gagaagcgca gccagtacct
     1741 gcgggacgga caggccgcat ggcagcacta tcaggaaacg ggtctgcacc ttactgcgga
     1801 agaagcggaa gcctggataa gtacctgggg aactgagaac gagcaggatg ctccgccatg
     1861 ccacaggtaa cgatttccgc gctggcacag cgcgatttac aacgccttca ggactttctc
     1921 aaaaccaaaa atcggctggc agccagaaaa ggaggtgagg tgatcgtccg ggctatccag
     1981 caactgaaaa cattgccaga cattggccgc ccggtgccgt ttctgccgct ggaatataag
     2041 gaactggtga tcgggtttgg cgacagtggc tatgtgatgc tctaccgcca cgaccgggaa
     2101 atggaccaga ttgtgatcgt caccgtcagg catcagaaag aatccgggta tccgggggca
     2161 gacagcctct aataaccgat gacgggggcg caattcattg cgtggccgtc gccggtttga
     2221 caagcgcagc gcgtcagaaa accacatcct gaacggaaaa acgggtaaaa ttcagcgata
     2281 catttaccgt gagaaattac acacctttca cacccgataa aatcaggggt atacagtggc
     2341 gaaaacattg ctcaaccttt cacaggcagc acaagccgcg ggcattacca gaagaacgct
     2401 atacaatcat gtaaaacagg gaaaggttac cgtgtcacgt gacgggaaaa acaaccctgt
     2461 cgtggatgtg tcagaactga tccgtgtata cggaaatgtg aatatccctg agaaacaaat
     2521 tcccggcatt tcacaccgtg aaaatacaca aaaaaatttc ccacaggaac agcttctggc
     2581 aatgcaaaag gaactggccg atctccggca ggctgtcacc ctgatgctgg aagataaaac
     2641 atcccgggaa gaagaacgcc gccagcacga tgatgagcgc cggaaactcc aggccgaagt
     2701 ggacagattg acaacagaac tgacacagaa gaaaaaacgc ttctggtccg gctggtttag
     2761 ctgagaactt accgaaggga atggagaaca tgacgaagca ggaaaaaact gccctcaata
     2821 tggctaaatt cattaaggat cagtcgctgc tgttgctgga aaagctgaac gagctggaca
     2881 tggatacccc ggccgacatg tgcgaacggc ttcacgagga tgcggaaaca ctctggcagg
     2941 tgatgcggga taagctcggg gaagaataac agcaccggcc atgccggtgc tggcgttatt
     3001 ttttgccgaa cggccaccac cggcgggcgg gtttgtcctc cggcagttca ggcaccggca
     3061 cgttaaaata gcggctgtta tattcccgca tcagctccgc tttcagcttc tcgttcaccg
     3121 cgctttcccc gtgccgctcg ctgaccacct caatcatcgc gttaatctgc tccggtgagc
     3181 acttcccggc atcaaccacc tgcacaccgt tctcgatggc gaaccgctcc acctttcgat
     3241 agaggtaatc acacagcttc attcctgtgg gtttgtcccg ttcccattgc ggattttcac
     3301 cggtgaaaaa gtacgtggct ttttcgtcgc tgttgagccg ctcgtcatac atcccgatgg
     3361 atttcatcat cgccagatgc tgggcccggt cgacaaaaca gaaaacatac gccccggtct
     3421 gccaggcgag caccaccatg tcttccagat gctcgctggt tggcataatg cagcgctcgc
     3481 cgtctttgtc ttccacgtcc tcaataaata ccatgtacag cgccataatc cctcactccg
     3541 ttgtcataaa tttaaagcac gcgaacagcc tctcccggtc cagctccacc gttttgcggg
     3601 cctcgccggt ctgtggattc acgatttcat ccaccgccag aatcgcccgc tgtacccgga
     3661 caagcgtcgg ccggtcaggg cagatgtacc agatttcatc gatgccgttc gcccggcggt
     3721 tgaacagata cccggacacc acttcctgat accgcctgcg gctcttgacg gtgcgctcta
     3781 cctcaaatgc caccgttctg ccgtccagct ccgccagcgc gtccggcact ttcatccctt
     3841 tcagggccat acggtgcagg taccgccagc cggtggcccc ccggccctcc atggagaggc
     3901 gcagggactg cacggccagc cggtgcggca gctgcgccgc cgacaccctt gacggctgaa
     3961 acggaataac gtccgtcagc ggctcgtcct cgtcgaacga gaacgccacc ccgtgcggcg
     4021 tcagccccca cacgctgacc tgacggccgt caaccgggta tttcgcccgg accagcagcc
     4081 ctttcgccac cagactgtcc aggaagcggt aaaccggctg taccgccgca atccccagcc
     4141 gtcgcgcaac cagctccgcc gtggaacaca gctcttcccg cagccagcgc agggtcagcc
     4201 gtgttttctc cgcgttccgc gcctgccgct tcgcgtggct gacgttgtac tgctccagtg
     4261 tcatcagtaa agccccgcaa aatcatcatc cgcttttggt ccgccgccgt cggccggagg
     4321 caggtactcc gccagcgggt ccgttttcgc cagcaccggg aaggttttca gcgaaatttc
     4381 ccggcgctca acccgcagcg ggctgctgaa cgccagccgc gccacgccca gcccggtcat
     4441 caccgcgaac ccgtcaggca ggtgttgcag ggtgttcacg tcaaacagcg gccgcgcctc
     4501 cttctggatg aagctgtcgc cgctgatgtg ctccacgttc cccgcctccc ggctggcccg
     4561 gcggcgctcc acgtccaccc ggatctcccc ggtctggccc gccgcccact gcgcggactc
     4621 ctggctggcc gcgcgataga accagcggat cggcgtgtta tccagtaccg tggttttcac
     4681 aaaatcagcg ggcagatcct gcccgcactg accgaagtcg cccagcgact ggtgcgcgag
     4741 cagcaggttg cagtcccggc tgcgcagcgt ccccagcgcg ttcagcacgt acttcgacag
     4801 cagatattta atctcatcga gcatgatgct cgcgtgcggc cacctccgga actcatcccg
     4861 cgcaatgatg atttgcgcgc agcgggcaaa cagcatcttc tgcacccgga tcaccgcctc
     4921 atcgtccatc gagccgatga cgtacaggca gccgccgccg ttgattatcc ccgccacatc
     4981 gataccgctg tccgtctgca ggacgctcag gtcggccacg ttctccagct tggtgatcag
     5041 ccccttcacg tcctttttca gggcctccgg caacgcgtac gccgccgcca gcacctgctg
     5101 aatgtttgcc ccctgcggga actgctccgc aatcagcttc gccgctttct gctcgctgat
     5161 gcggtagtgg tccgccacgt cgccggtctc actcaggtta aacccggcaa ccagcagatt
     5221 cttcagggcg tactggtcga gatcgcggaa cagattcagc tgcgccggtt tccccggccg
     5281 cagatccagc agggtgaagt ccgggcagtg cgcccggaag acgtgcggcg cccacgcgtc
     5341 ccctttgggg tcgaagtaca ccacagcgtc cccgaaatcg cgtacgcagc gaatgagcgc
     5401 gttggtggcc atcgcgctct tgccgctccc gggcaggccg aggatctgaa tattggtctt
     5461 gcgccaggta tcacgaggga ccagcaccgg ctggccctgc tcatccttac ccagaaacac
     5521 cgcctgctgt ttccgggcct gctccgtgga aaaatcggcc agcgcgttaa ccggtaccgg
     5581 ctccccgaaa tgcaggtggc ggacgtctgt catttctccg gtccgctggc tgctgcgccg
     5641 gctctcctga ttcagtttcg cgttcaccgc ctcgtcagcg gccgcaaaat gaccatcaag
     5701 cagcaggaac gccagcagcc gcgccagaag gacgccggcg acaagccctc cggcacacca
     5761 ggctatcccg aacaggttag ccggctgcag caggtaataa tctgcgttcg catagaggtc
     5821 cgggcgcagg acggtttctc cccagctctc gataaacacg gcgacaacca gcccccccag
     5881 aagggccaga ccggcagcgc cggtcagcca gtacgcgtag ttccagacat gtcgccgtgc
     5941 cctgaccatc cgcccgggtg aatccagcga ggccagcacc gaaaatggca cagtccggtt
     6001 attatcgcgc ttatagcgat cggcatactc ccgccgcgcc ttatggctca gccccacaaa
     6061 acgcattcgg ccggcccaga gttcagccag accgaaaaac aggccgataa ccagaaaaaa
     6121 accggccaga ctgtaatact cccggtacag ccagctgacc cacgggtaaa tctgccgggc
     6181 cagctccggg atatgccact gccaggcaag cgaatccgta ttaaacatgc aatctctctc
     6241 cttcagacga cgggtttacc ggcgtgatgc gcagcccccg ctgcacaagc cgcggacatg
     6301 ccccaaggca tggcggcggc ggtcacgtcc gtggtgattg cgttcagctt tcaccgcgga
     6361 ggccagcctc tttccgcccc atgcggaaaa ggcgcagccc cggaggaaca acgagcacgc
     6421 cccagcgtgt gagaggaaaa ggcggcaccc cagtgcggtc ttttccggtg ttccggcccc
     6481 cgtcagagtt cgccatagcg agcggctgac cacccgcaaa atcaggagca gctttgctgc
     6541 ttcgtcaggt gcgtccagca ccgtggattt ttgtgggtgg tcaggcgtga gcctgacggg
     6601 ggccgggtga atttctggcg tggccggcgt ccagccggac agagccagaa agaggggaaa
     6661 agaccgtgcg tcggtggcgt ccagccagcg taagcaaggt cttttcccct gaagtcagcc
     6721 acgccccagc ggggatgact tgcatttctc cgcaaggaga aaaaagcaac agagataggt
     6781 taacgtaaaa tgttcagcga aaaaccgaca tggttcacct atcctgataa ttgatcgtca
     6841 gggaatagaa agacttaatc aggggacaat atcccacatc agcgcgtccg gtgaccgtct
     6901 tgcaggtgcc ggggaaattg cgcctcctcg ctcactcggt cgctacgctc cgggcgtgag
     6961 actgcggcgg gcgctgcgga cacatacaaa gttacccaca gattccgtgg ataagcaggg
     7021 gactaacatg tgaggcaaaa cggcagggcc gcgccggtgg cgtttttcca taggctccgc
     7081 cctcctgcca gagttcacat aaacagacgc tttttcggtg catctgtggg agccgtgagg
     7141 ctcaaccatg aatctgacag tacgggcgaa acccgacagg acttaaagat ccccaccgtt
     7201 tccggtaggt cgctccctct tgcgctctcc tgttccgacc ctgccgttta ccggatacct
     7261 gtccgccgtt ctcccttgcg ggaagtgtgg cgctttctca tagctcacac actggtatct
     7321 cggctcggtg taggtcgttc gctccaagct gggctgtaag caagaactcc ccgttcagcc
     7381 cgactgctgc gccttatccg gtaactgttc acttgagtcc aacccgggaa agcacgcaaa
     7441 acgccactgg cagcagccat tggtaactgg gagttcgcag aggatttgtt tagctaaaca
     7501 cgcggttgct cttgaagtgt gcgccaaagt ccggctacac tggaaggaca gatttggttg
     7561 ctgtgctctg cgaaagccag ttaccacggt taagcagttc cccaactgac ttaaccttcg
     7621 atcaaaccac ctccccaggt ggttttttcg tttacagggc aaaagattac gcgcagaaaa
     7681 aaaggatctc aagaagatcc tttgatcttt tctactgaac cgcgatcccc gtcagttcag
     7741 aggaggacga tggtgccacc gttccgccct gaacatcccg gatataattt gcctgacatg
     7801 cggccagaag aaagccagca acaatcaata taaaaagaaa aatagtcttt acctttttca
     7861 tatactttcc ctgtatttaa agactccggc attcatccct gaacaccgga agtcagtatt
     7921 tcaaatcatc cttgataatt ttttgttacc agttaccgtc ccgataatca aaagctatat
     7981 aacaattatc cacatcaggt tttatttgat ttttaaaaaa tggctgtagt aaaggaaccc
     8041 attctttctt caaatcaaac tcaccattat taatattatc ctttaaaggc attcccaagc
     8101 tttcaatgac agaaccatcg tcaccaaagt ctttagaata ctcttcccct ttaaactctt
     8161 cagttttctt atcaaaccat gtcaaacgta atttaagccc catatttacc tctcaaagat
     8221 attttttaat gttccgtttt ggatctggcc ctttaacctg cttacccgtt ttgggatcaa
     8281 atgcgcctag gtgttcgcca tcactggcac gatacccttc aagctcaccg tgctgggaat
     8341 cccattcata aattttacgt tttttatcgc cataccatcg ggcccgctta ccaccaccac
     8401 cttgtttagg tgttttagga tcacctcgct ttaactctcc aagtcccttt atgtcttctg
     8461 ttttaggtac tggatgataa tcatggccgt agtctttagt ccccttgcga ggcttgttct
     8521 tttcctcatt taacttattt tctgcatcct tcgctttttt ctccttttgt ttccggctct
     8581 ccatcgcccc ggtaagtgca gccttatctc tggataagga ttcattcatt gcgtttaaag
     8641 atgcctgagc cgcttttaat ttctcattct gattctgaat atccttatca agatagcgaa
     8701 cctgatcctg ccacccggag ccatattcat gaggcgggtt ataagtcaca aaatcatctt
     8761 tatttgcctc tgcctctttt acctttttat cggcttcctg aacagcagcc cttgcaccgg
     8821 ggagcgcctt ctctgtcgat gcaatgcggc tgtttaaggt cgcaatattt ttatcttcgg
     8881 catccagctc agctttcgct ttatcatagt ctcttttcag cccctcttcc ggatgagcgg
     8941 cgtcccatgc ctgctggcga cgcttttctt cctcctgacg ctgttttacc tgtgccgggg
     9001 taagaacatc tgtcaccgac acataaaccg gcttctgtcc ggtctctttc gggaaacgaa
     9061 taacggcctc acgggagtta ccaccggctg taaaaccagc cggacgtgaa tcaccttttt
     9121 cagcaacaat gccttttggc ggggctttcg ctgtcggaac acctttcggt acgctcacct
     9181 gcagagacgg gagacccgga atcgacacac tgaatacccc cggacgtttt gtcggtttcg
     9241 catccaccac agggacactc attggccgac ctgcgacaac cgcaatatgc tgccgctcat
     9301 ccttcaccac atccacaacc cgttgtctga cacgaaccgt cgcctggtcc agtggtaaag
     9361 tacttgccgg agtctccgtc accgcatcgg ccggtaatga cgtcataatt tttgacatca
     9421 ttttcggatc atcttttgca atctcagaag gcagcacacc gtagatcgca atcccccaca
     9481 gaccaaactt aaacggcccc ttcagggcag ccagcacatc agcaacggcg gccgacaacg
     9541 catcgccgga aactgataaa gccagtcctt cagcccccgg agtagccagg gcaggcagac
     9601 cgaaggccat cgcagtggcc gaagactgtc cgccaccaga gctaccgcta tgattgccgc
     9661 tgttaccacc attaccaccg ggtttactcc cgccgttacc aaactccccc ggattatagt
     9721 tatgaatatc tccattcggc gtatgcgtcg taccccagcc cggcccgctg tttggatccg
     9781 taccaccacc tgatgaaggg ccacctttac cagacgtacc gttcacattc ccactggcct
     9841 gaccaccatt atgtcccaga cctgaattac ccggacctcg accgtctcca ccactcatac
     9901 cgttttcctc tttgacattt aaaacaataa attaaagcca acatactgta catatacaca
     9961 gtataattat taccttactc ctcccgtttt caggtgtcaa tatatgagat tagttctcat
    10021 cttcaggcac aaaaaagcgc ccggagccac ctgtaagcag
//
