LOCUS       NZ_CP081795             2725 bp    DNA     circular CON 02-NOV-2023
DEFINITION  Escherichia coli strain CT01 plasmid unnamed4, complete sequence.
ACCESSION   NZ_CP081795
VERSION     NZ_CP081795.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN18446033
            Assembly: GCF_019969605.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2725)
  AUTHORS   Yang,X., Tran,F. and Zhang,P.
  TITLE     Genetic characterization of Escherichia coli recovered from beef
            production environments, which harbor the locus of heat resistance
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2725)
  AUTHORS   Yang,X., Tran,F. and Zhang,P.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2021) Food Safety, Agriculture & Agri-Food Canada,
            6000 C & E Trail, Lacombe, Alberta T4L 1W1, Canada
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 26-JAN-2021
            Assembly Method                   :: flye v. 2.8.2
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 78x
            Sequencing Technology             :: Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/02/2023 03:49:47
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,128
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,706
            CDSs (with protein)               :: 4,706
            Genes (RNA)                       :: 126
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 90
            ncRNAs                            :: 14
            Pseudo Genes (total)              :: 296
            CDSs (without protein)            :: 296
            Pseudo Genes (ambiguous residues) :: 0 of 296
            Pseudo Genes (frameshifted)       :: 69 of 296
            Pseudo Genes (incomplete)         :: 224 of 296
            Pseudo Genes (internal stop)      :: 41 of 296
            Pseudo Genes (multiple problems)  :: 36 of 296
            Pseudo Genes (short protein)      :: 3 of 296
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP081795.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2725
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="CT01"
                     /db_xref="taxon:562"
                     /plasmid="unnamed4"
                     /geo_loc_name="Canada: Alberta"
                     /collection_date="2013"
     gene            complement(17..1033)
                     /gene="mobV"
                     /locus_tag="J6O14_RS25485"
                     /old_locus_tag="J6O14_25370"
     CDS             complement(17..1033)
                     /gene="mobV"
                     /locus_tag="J6O14_RS25485"
                     /old_locus_tag="J6O14_25370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000024818.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobV family relaxase"
                     /protein_id="WP_136655212.1"
                     /translation="MRSPDWLDGRQNEGVSMANYAIMRCKKLTGMGSVASALQHCYRER
                     ETPNADAERTPENYCSVSQSADEAMGKLRELLPEKRRKDAVLAVEYVMTASPEWWKEAT
                     PRQQAEFFARSEQWLEKKYGKDRVVAAVVHRDEATPHLSAFVVPLTQDGRLSAKEFIGG
                     RSKMRDDQSTYAESVKKLGLERGIEGSRATHQTVQHYYESINRGTRSQVSISPEALEPR
                     VLRKGIFTKDVEDQAAIAKRLSHAVNDGFAGTIAMASQSAQNAKRARELQKTMDSQQKR
                     LQSVTEPFKGLSREQMTEILMMAQRFKQQNQEKEKQQRVEREKQRQMRSRGMGGMER"
                     /name="mobV"
     gene            1235..1840
                     /locus_tag="J6O14_RS25490"
                     /old_locus_tag="J6O14_25375"
     CDS             1235..1840
                     /locus_tag="J6O14_RS25490"
                     /old_locus_tag="J6O14_25375"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_054623596.1"
                     /translation="MSFIKSLNKKNQTDQIEVTINVRLTQEEDHLLTLATQQSGYKRNE
                     LFYLLAKEYIFDDESLKEMAEESGAEFSEGLYQNCYVVNTNRVHHEEDDKFMISEGVAA
                     TFEDGYKEKMEQVKKGDLVFLYRSGEGIVAHGIASGDVLKKDHNGVPDKTYYQKLSGFV
                     RYEKPLTASEIQRILGRNVVFMHTLFSIRDGYKLLKKD"
                     /name="hypothetical protein"
     gene            2360..2686
                     /locus_tag="J6O14_RS25495"
                     /old_locus_tag="J6O14_25380"
     CDS             2360..2686
                     /locus_tag="J6O14_RS25495"
                     /old_locus_tag="J6O14_25380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001070513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_223571429.1"
                     /translation="MNQAALITIANELNHDQMRVLMALLAELDYENYIQVAQMDIAEAL
                     TMQKTNVSRAVKNLIDFGIILEGPKIGRSKTYRLNPQFGWKGTVSNHKKALKNGLSVIQ
                     GGRT"
                     /name="helix-turn-helix domain-containing protein"
     Typing          complement(21..1033)
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="97.337"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 ctgcatcgca tcaaaactaa cgctccatac cgcccatgcc acgcgatcgc atctgtcgct
       61 gtttctcacg ttcaacacgc tgctgtttct ccttctcctg gttttgctgc ttaaaccgct
      121 gagccatcat caggatttct gtcatctgct ctctggaaag ccccttaaaa ggctctgtaa
      181 cgctctgtag gcgtttctgc tgcgaatcca tagtcttctg tagctcgcgc gctctttttg
      241 cattctgggc gctctgagag gccatagcga tggttcctgc aaagccatcg ttcactgcgt
      301 ggcttagacg tttggctatg gctgcctgat cttccacgtc tttggtgaaa atgcccttcc
      361 gcaatacacg cggctccagc gcctccggcg agatcgatac ctggctacgg gtgccacggt
      421 ttatcgattc gtagtagtgc tgaactgtct gatgcgtggc gcggcttccc tcgatgccgc
      481 gctcaagacc cagttttttt actgattcag cgtaggtgct ctgatcgtca cgcatctttg
      541 accgcccgcc aataaactct ttcgcggaca gccgtccatc ctgcgtcaat ggcaccacga
      601 acgctgacaa gtgcggggtg gcctcgtccc ggtgaaccac cgccgccaca acacgatcct
      661 tcccgtattt cttctccagc cattgctcag agcgggcgaa aaactccgcc tgctgccgcg
      721 gtgtcgcttc tttccaccac tcagggcttg ccgtcataac gtattcaaca gccagtaccg
      781 catctttacg gcgtttttcc ggcaacagct cccgcagctt tcccatcgcc tcatctgccg
      841 actgagacac agagcagtag ttttctggtg tccgttcagc gtcagcattc ggcgtctcac
      901 gctccctgta gcagtgctgc aaagcgctgg ccacactgcc cattcccgtc agctttttgc
      961 agcgcattat cgcgtaattc gccatgctta ccccctcgtt ctggcgtcca tccagccagt
     1021 cgggggatcg cattgcgatg cctccggcgg gccactatca ggatgatagt gtactcacta
     1081 gactttgcta cgcaaaatcg ttcgttaaat tcaacttaat aaaaatatat gacataaagt
     1141 cattgcttaa atcaaagatg tgatcaatgc ttctgtttca atgtagtgat tcactatcat
     1201 tgattagtac attaactctg gtgaggaaag taagatgtca ttcataaaat cgctaaacaa
     1261 aaaaaatcaa acagaccaaa tagaagtgac gattaacgtt aggttaaccc aagaagagga
     1321 ccatttactg acattagcaa cccagcaatc tggttataaa aggaacgagc tattctacct
     1381 cctagcaaag gaatacatct ttgatgatga aagcttaaag gaaatggctg aggagtctgg
     1441 cgctgagttt tcagaaggct tataccaaaa ttgttatgtg gttaacacta acagagttca
     1501 tcatgaagaa gatgataagt tcatgatttc agagggcgta gcggcaacat ttgaagatgg
     1561 ttataaggaa aaaatggagc aggttaaaaa aggagattta gtctttcttt atcgctccgg
     1621 cgaaggaatt gttgcacatg gtatcgccag tggtgatgtt ttaaagaaag atcataatgg
     1681 cgtgccagac aagacctact accagaagct atcaggcttt gttaggtacg aaaaaccact
     1741 aaccgcgtct gagatccaga gaatactggg gagaaacgtt gtgtttatgc acacgctatt
     1801 tagcattaga gatggctata aactgcttaa aaaagattaa acccagtcta caggtggttg
     1861 gcatcacttt gtgacaccaa ccaccttccg gaagtgtttt ttacagtgca tcgtcaaggg
     1921 taaatgaact cgctacgctc gcccttgacg aaataaagct ccgaatatcg ttttaaaaaa
     1981 tcaagaaccg ctcgccgcag actcatgacc gaacgtagtg agcgaatgag cgaggaagcg
     2041 gaatttccac ttcccgtaca tagaaattcg ttcgcaccga acgggcagaa aaacgcctga
     2101 ttttgccgat gtgtgattgt ggagagtaat aagatacaga aggggatggc tgggaagccg
     2161 cgccacaagc ggaaagtttt tgacaatagt tatctctaga gataacttag ttatcttgag
     2221 agataactat tttttcctga atggagattt ttagatgcgg agaatcacgc aggtagacca
     2281 aactacaggg gaggaattag gtggttttgt agcagtgatt cgtcctaaac aaaagtcatc
     2341 attccagagg cactttacta tgaaccaagc cgcgctaatt accattgcca atgagctaaa
     2401 ccatgaccag atgcgtgtgc tgatggcctt gctcgcagaa ctggattacg agaactacat
     2461 ccaagttgcg cagatggata ttgcggaagc gctgactatg caaaaaacaa atgtcagtcg
     2521 tgcggtaaaa aacctgattg atttcgggat catactggaa gggccgaaga ttgggcggag
     2581 caaaacgtac cgcctaaatc cccagttcgg ctggaaaggt actgtcagca accacaaaaa
     2641 agcactgaaa aacggcctca gcgttataca gggcggcaga acctgatctc tggaatgccg
     2701 caaatggcgt cattttgcgg cgtta
//
