LOCUS       NZ_CP081766             3215 bp    DNA     circular CON 02-NOV-2023
DEFINITION  Escherichia coli strain CX21 plasmid unnamed4, complete sequence.
ACCESSION   NZ_CP081766
VERSION     NZ_CP081766.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN18446050
            Assembly: GCF_019971115.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3215)
  AUTHORS   Yang,X., Tran,F. and Zhang,P.
  TITLE     Genetic characterization of Escherichia coli recovered from beef
            production environments, which harbor the locus of heat resistance
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3215)
  AUTHORS   Yang,X., Tran,F. and Zhang,P.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2021) Food Safety, Agriculture & Agri-Food Canada,
            6000 C & E Trail, Lacombe, Alberta T4L 1W1, Canada
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 26-JAN-2021
            Assembly Method                   :: flye v. 2.8.2
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 112x
            Sequencing Technology             :: Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/02/2023 04:13:32
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,195
            CDSs (total)                      :: 5,080
            Genes (coding)                    :: 4,832
            CDSs (with protein)               :: 4,832
            Genes (RNA)                       :: 115
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 248
            CDSs (without protein)            :: 248
            Pseudo Genes (ambiguous residues) :: 0 of 248
            Pseudo Genes (frameshifted)       :: 65 of 248
            Pseudo Genes (incomplete)         :: 171 of 248
            Pseudo Genes (internal stop)      :: 47 of 248
            Pseudo Genes (multiple problems)  :: 34 of 248
            Pseudo Genes (short protein)      :: 3 of 248
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP081766.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3215
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="CX21"
                     /db_xref="taxon:562"
                     /plasmid="unnamed4"
                     /geo_loc_name="Canada: Alberta"
                     /collection_date="2014/2015"
     gene            complement(339..473)
                     /locus_tag="J6O46_RS26045"
                     /old_locus_tag="J6O46_25660"
     CDS             complement(339..473)
                     /locus_tag="J6O46_RS26045"
                     /old_locus_tag="J6O46_25660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_072035952.1"
                     /translation="MHEPPVQSDCCALSGNYRLESNPERHDKAPLAAATGNRFRGGES"
                     /name="replication initiation protein"
     gene            1327..1554
                     /locus_tag="J6O46_RS25765"
                     /old_locus_tag="J6O46_25665"
     CDS             1327..1554
                     /locus_tag="J6O46_RS25765"
                     /old_locus_tag="J6O46_25665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001046776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_206659768.1"
                     /translation="MFGFSPGELMNLCPDERLLFVRMISAMLRRSGGDAGAVMFEAYRH
                     IVSDTNQARRSCMLDLLESVRHDYVHGGYT"
                     /name="hypothetical protein"
     gene            complement(1602..1787)
                     /locus_tag="J6O46_RS25770"
                     /old_locus_tag="J6O46_25670"
     CDS             complement(1602..1787)
                     /locus_tag="J6O46_RS25770"
                     /old_locus_tag="J6O46_25670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_054626234.1"
                     /translation="MTKQESAALNMAKFIRAQSLLLEKLDVLDLDDEAADCERMHEQAE
                     ALYQKLSARFGIQDGE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            2333..2557
                     /locus_tag="J6O46_RS25775"
                     /old_locus_tag="J6O46_25675"
     CDS             2333..2557
                     /locus_tag="J6O46_RS25775"
                     /old_locus_tag="J6O46_25675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6290 family protein"
                     /protein_id="WP_000879771.1"
                     /translation="MLAIRLSDEIESRLDSLAKQTGRTKTFYAREAILAHLEDLEDYYL
                     SAETAARVRRGDEAVHSSEDVRKSLGLDD"
                     /name="DUF6290 family protein"
     gene            2541..2807
                     /locus_tag="J6O46_RS25780"
                     /old_locus_tag="J6O46_25680"
     CDS             2541..2807
                     /locus_tag="J6O46_RS25780"
                     /old_locus_tag="J6O46_25680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262326.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_000262326.1"
                     /translation="MVWTINYSDRALKSLRKMDKQNARRIVDFMDLRIAVAADPRQSGK
                     PLKGELGEFWRYRVGDYRILCEIRDDELVILAATIGHRREVYD"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            2917..3129
                     /locus_tag="J6O46_RS25785"
                     /old_locus_tag="J6O46_25685"
     CDS             2917..3129
                     /locus_tag="J6O46_RS25785"
                     /old_locus_tag="J6O46_25685"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_039509481.1"
                     /translation="MGDFERTYGAGADFDRIIDENNSEYFRDERQKAKNSKKEFSSFQD
                     ALVWAKANPGKTIVRSPDGVGFIEK"
                     /name="hypothetical protein"
     Typing          complement(365..854)
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="96.13"
                     /coverage="81.0"
                     /evalue="0.0"
ORIGIN
        1 tgttggagtc tatgttacgt acattgcagg gcctattttg ttagttacag gaattcttgc
       61 atggcttacg aaacctagta gtaattcaaa taaatctaat atgtgactgg gtaagacgaa
      121 cttcatccaa ccataaaacc cgctttttta aacggatctt cttgagatca tttagggaga
      181 atcgtaatct cttagctctg taaacgaaaa aaccacccgg caaggtggct ttttcgaagg
      241 ttcgcaagag ttgacgctct tttgaaccgc ggtaactggc ttggaggagc acagtcacaa
      301 aatagtcctt tcagtgtagc cttatcgcac ccgaaatttc aagactctcc tcctctaaac
      361 ctgttaccag tggctgctgc cagtggcgct ttgtcgtgtc tttccgggtt ggactcaaga
      421 cgatagttac cggataaggc gcagcagtcg gactgaacgg ggggttcgtg catacagtcc
      481 agcttggagc gaactgccta cccggaactg agtgtcaggc gtggaatgag ataaacgcgg
      541 ccataacagc ggaatgacac cggtaaaccg aaaggcagga acaggagagc gcacgaggga
      601 gccaccaggg ggaaacgcct ggtatcttta agtcctgtcg ggtttcgcca ccactgattt
      661 gagcgtcaga tttcgtgatg cttgtcaggg gggcggagcc tatggaaaaa cggcttcgcc
      721 gtggccttct tgcttccctg ttaagcttct tcctggaaga tgccaggaaa tttccgcccc
      781 gccagtaagc cggtaccgct cgccgcagcc gaacgaccga gcggagcgag tcagtgagcg
      841 aggaagcgga atatctccgg gcttatcttt ggcaccgtac gcgccatagc tgcatttttt
      901 aatacgatgc aggatagggc aaacgccgca aaatgacgtc atccgacatc attcctccgc
      961 gttcgtccag tgacctacag tcggctctgg ggtgtcgggg ctgtgccctg accaggcgtt
     1021 ttggagtatc gccaccgtgg tggcggtgct acaacacata tcctgtccct tccttggcat
     1081 ctctgaagtt tagccagtat acactccgct agcgctacgt gactggttca gggctgcgcc
     1141 ccgacacccg ctaaaagcac tgacgcgcct gcggcttgcc caaccccgcg ccgtccggga
     1201 aagaatttcc cgatcgaccc gacgtttctg tttacctgtg atgttaacgt ttggatcttt
     1261 ttcccgggtt tacctatttt cttctaatga agcgttcaca gctgacgtta atagatgggt
     1321 gctttcatgt ttggtttttc accaggagaa cttatgaacc tttgccccga tgaacgtctg
     1381 ctctttgtgc ggatgatttc cgctatgctt cgccgttcgg gcggtgatgc gggtgctgtt
     1441 atgtttgagg cgtatcgcca tattgtgtcc gataccaatc aagctcgtcg ttcctgcatg
     1501 ctcgacctgc tcgaaagcgt caggcatgac tatgtacacg gtggatatac atagttgccc
     1561 tggatacgct tttcgtctaa gtgagcgttc ttttcgtctc tttattcgcc gtcctgtata
     1621 ccgaagcgtg cgctcagctt ctgataaagc gcttctgcct gttcgtgcat tcgctcacag
     1681 tcggctgcct cgtcatcaag atcgagcacg tcgagttttt cgagcagcag cgactgcgcc
     1741 cggataaatt ttgccatgtt cagtgcggct gattcctgtt ttgtcatacg gctttttccc
     1801 ggttttatgg tgatcgcaga ctgaaatctt acttcattca cttgagtgca tagccatgca
     1861 ggtacgcaga aatcatcagc gcgtttctga gcggttttca gggggtaaga tgtgttcttt
     1921 tgcaggtgag gtcgtagaaa cgccctgaga gcctctcagg cggtgcacgc aacagatgtt
     1981 atgttcaatc agactgatct gcgggtacaa aaaaagcccc ggagggcttt taactactga
     2041 cttatttttt tgaggatttg ctgggtttca atttgtcagt attccccggc gtttttttat
     2101 tgtcacgctg gcgtttatca ggttcccctg ttgaatcggc aattcgtttc ggacccggct
     2161 taagacccat aatcgttatc ctttcaatag ttaagaggaa agccctcaag ctggatatat
     2221 tgacagcgtt acggattatc aagaggggcc agcaagatta ttttttgttc ttttaggttg
     2281 attgcgatac tattgctata catgtatatc aaatggtgtt caggaggttt atatgcttgc
     2341 tatccggtta tctgatgaga ttgagtcccg tctggactcg ctggcgaagc aaaccggcag
     2401 aacaaagacg ttctatgcgc gggaagcaat actggcgcat ctggaagacc tggaagatta
     2461 ttatctttca gcagaaactg ccgcacgcgt tcgccgtggt gatgaagccg tgcattcgtc
     2521 tgaagacgtg aggaagtcac ttggtctgga cgattaacta ttccgaccgg gcgctcaaat
     2581 cgttacgcaa gatggacaaa cagaacgcac gacggattgt ggattttatg gatttacgca
     2641 ttgcagttgc tgccgatcct cgccagtcag ggaagccgct caaaggtgag ctgggcgagt
     2701 tctggcgcta tcgggtggga gattatcgca ttctgtgtga gatccgggat gacgagctgg
     2761 ttatccttgc cgccaccatt ggccatcgcc gcgaagttta cgactgaagc ccgctatgcg
     2821 ggcttttttt gatgctgaca acggatgtta tggtaaataa gagtgtctgt tggatatttt
     2881 aaatctgttt ttttattaaa tttatggggg taggaaatgg gggattttga gcgtacctac
     2941 ggagctggtg ctgattttga tcggataatt gatgagaata acagcgaata ttttcgagat
     3001 gagcgccaaa aagctaaaaa ttcgaaaaaa gaattctctt cttttcaaga tgctttggtt
     3061 tgggcgaaag ctaatcctgg taaaacgata gtaagatctc cagacggtgt gggatttatt
     3121 gaaaaatgaa agagtggatt cactgtaaaa cggcagtcat tttttggaca acactggcta
     3181 tatacgtact catatgtttc acagtgtcgt atgtt
//
