LOCUS       NZ_CP081765             4075 bp    DNA     circular CON 02-NOV-2023
DEFINITION  Escherichia coli strain CX21 plasmid unnamed3, complete sequence.
ACCESSION   NZ_CP081765
VERSION     NZ_CP081765.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN18446050
            Assembly: GCF_019971115.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4075)
  AUTHORS   Yang,X., Tran,F. and Zhang,P.
  TITLE     Genetic characterization of Escherichia coli recovered from beef
            production environments, which harbor the locus of heat resistance
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4075)
  AUTHORS   Yang,X., Tran,F. and Zhang,P.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2021) Food Safety, Agriculture & Agri-Food Canada,
            6000 C & E Trail, Lacombe, Alberta T4L 1W1, Canada
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 26-JAN-2021
            Assembly Method                   :: flye v. 2.8.2
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 112x
            Sequencing Technology             :: Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/02/2023 04:13:32
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,195
            CDSs (total)                      :: 5,080
            Genes (coding)                    :: 4,832
            CDSs (with protein)               :: 4,832
            Genes (RNA)                       :: 115
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 248
            CDSs (without protein)            :: 248
            Pseudo Genes (ambiguous residues) :: 0 of 248
            Pseudo Genes (frameshifted)       :: 65 of 248
            Pseudo Genes (incomplete)         :: 171 of 248
            Pseudo Genes (internal stop)      :: 47 of 248
            Pseudo Genes (multiple problems)  :: 34 of 248
            Pseudo Genes (short protein)      :: 3 of 248
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP081765.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4075
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="CX21"
                     /db_xref="taxon:562"
                     /plasmid="unnamed3"
                     /geo_loc_name="Canada: Alberta"
                     /collection_date="2014/2015"
     gene            join(2981..4075,1..24)
                     /gene="wzz(fepE)"
                     /locus_tag="J6O46_RS25745"
                     /old_locus_tag="J6O46_25640"
     CDS             join(2981..4075,1..24)
                     /gene="wzz(fepE)"
                     /locus_tag="J6O46_RS25745"
                     /old_locus_tag="J6O46_25640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016243311.1"
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_process="GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen length regulator Wzz(fepE)"
                     /protein_id="WP_024191316.1"
                     /translation="MFSMKISKISDEHFSNYNMLPNGNEEIDLFDLIRVLWAAKIKIVG
                     VILFFACIGFLVSFVLPQKWTSSAVVTPAESVQWSELNKELSKLHVLGVDFDINRDSAF
                     ALFIKKFQSVNSLNEYMRSSPYVMEIIKKKNISALELHRAIVGLSENMKSVDDNASKKN
                     DKSSLYVSWTLSFTAPTSKEAHDVLSGYINYVSSLVVRDLMEDIRNELEVKTNVEKEIL
                     ALDEIKIRNQLNADIRRLNYSLEVANAAGIKKPVYSNGQIMKDDPDFPVALGSDGIATK
                     LNIKKSIKDVSELSGELRNRQYVVNQLVVAKVGDVDFMPFQYQLSPTLPVRKDGPSNAI
                     IVILSSVIGGMLACGYILLRHAMEARKMSVIE"
                     /name="wzz(fepE)"
     gene            961..1110
                     /locus_tag="J6O46_RS25750"
                     /old_locus_tag="J6O46_25645"
     CDS             961..1110
                     /locus_tag="J6O46_RS25750"
                     /old_locus_tag="J6O46_25645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017880354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001515742.1"
                     /translation="MPSEYDEQPDATDALFPGEYDLFDLMAHVTEENLHAPVDSGPPVG
                     REAL"
                     /name="hypothetical protein"
     gene            1579..1770
                     /locus_tag="J6O46_RS25755"
                     /old_locus_tag="J6O46_25650"
     CDS             1579..1770
                     /locus_tag="J6O46_RS25755"
                     /old_locus_tag="J6O46_25650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000166329.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELDLDVEADLCETLHDDA
                     EHLFRTLSSRLDALQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            1770..2171
                     /locus_tag="J6O46_RS25760"
                     /old_locus_tag="J6O46_25655"
     CDS             1770..2171
                     /locus_tag="J6O46_RS25760"
                     /old_locus_tag="J6O46_25655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310048.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_000135006.1"
                     /translation="MARVSISEAARLTGKSRTTLHRLIKAGDLSTCSGERNAKMVDTSE
                     LLRVFGPFEQPKGEHHSGQVSEQRDTASADQSEQVTRHLKQEIEHLNTLLLSKDSHIDS
                     LKQAMLLLENHQAKLTESPAPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     Typing          754..968
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="84.091"
                     /coverage="100.0"
                     /evalue="3.48e-51"
     Typing          complement(245..773)
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="84.545"
                     /coverage="89.0"
                     /evalue="1.2e-144"
ORIGIN
        1 agaaagatga gtgtgattga gtaaaagttg tgctgatgca tcattttata agcaactttt
       61 ataaaaagtt caaaggatct tcttgagatc attcccttac agccgtagtc ttctactcta
      121 gaaaacgaaa aaaccacctg gggaggtggt ttttcgaagg ttcagtaagt tggggaactt
      181 ctgaaccgtg gtaacagtct tgtgcgagac atgtcaccaa atctgtcctt ctagtgtagc
      241 ctcagtaagg ctgccacttc aagaactctc aatacatctc tcgcacatcc tgtttaccag
      301 tggccgctgc caatggcgtt aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
      361 cggataaggc gcagcggtcg ggctgaacgg ggggttcttg catacagccc agcttggagc
      421 gaactgtcta aacggaacgg ggcgtggtga tttaggtaaa gcttccacca ctacacggat
      481 gccgcaggac gggaacagga gagcgcaaga gggagccacc agggggaaac gcctggtatc
      541 tttatagtcc tgtcgggttt cgccaccact gatttgagcg tcagatttcg tgatgcttgt
      601 caggggggcg gagcctatgg aaaaacggct ttgccgcggc cttatcgtat ccctgttaag
      661 tttcttcctg gcatccacca ggaaataaat ttccgcccca ttcgtaagcc tgcaccgctc
      721 gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa tatcacctga
      781 accagcatct tctcctgacg ccggtaagcc tcatttcact cctgccacat gaagcacttt
      841 ggcaaatcac cacttcggtt taaaatattt agccagtatg cactccgcta gcgctacgtg
      901 actggttcag ggctgcgccc cgaaacccgc taacacccct cagctactgg atatcacacc
      961 atgccatctg agtatgatga acaaccggat gcgactgatg ctctgtttcc cggcgaatat
     1021 gacctttttg acttaatggc tcatgttact gaagagaacc tgcacgcccc ggtggattct
     1081 ggcccgcctg tcggccggga agcgttatag ctttttcgct ggccagcgta catgtaatac
     1141 gctggtatta catgttattt gtttattttc atgttgttac agatgattgt aatacgttgc
     1201 gtattacgct aatacacgaa ataactccat ttttttcatt taattcatgc agttgcgata
     1261 aaaatcgcgg ctgcgttagg tgtatgacca tttaaggggc aaaaaattat cattctggct
     1321 gggtgatctg cgcaggttaa ggatcatcgg cgacgaaaaa gcgtctttaa ctgcttcgtt
     1381 catgcaagca tgaacggatt atttttccag actacaggaa gactacaaaa gttgatgatt
     1441 taatcctgcg ttttagtgcg atgactacag ataaactaca aaataactac aggataacct
     1501 cagatccgtt ctctgaacca ttcgtacatc cgaccccctt accggtttat actgagcact
     1561 gatgctctga gggcccttat gacgaaacaa cagaaaacag cgctgaacat ggctaaattc
     1621 atccagaatc agtctctgct cctgctggag aagcttaacg agcttgatct cgatgttgag
     1681 gcggaccttt gtgagacact ccacgatgat gcggagcatc ttttccgtac actgtcctcg
     1741 agattggatg cattacagga tggtaactga tggccagagt atctattagt gaggctgccc
     1801 gactgacggg taagagtaga acaacactgc acaggttaat taaagcaggt gacctgtcca
     1861 cctgtagcgg tgaacgcaat gcaaaaatgg tagatacttc agagctttta cgtgtctttg
     1921 gtccctttga acaaccgaag ggtgaacacc attctggaca ggtctctgaa cagcgtgata
     1981 cagcctctgc tgaccagagt gaacaggtga ctcgtcatct gaaacaggag attgaacacc
     2041 tgaacacctt acttttgtcc aaggattcgc acattgacag cctgaaacag gctatgctcc
     2101 ttcttgaaaa tcaccaggca aaattaactg aatccccggc gccatggtgg aaattctgga
     2161 aaaaatcctg accccgtcat gcttcagttt tcctctccgg ccatcccggg gagttcctga
     2221 accttcccaa aaatcgtcag atctctgaac gctcctcacc actaaaaata cccacaccct
     2281 acgcaaaatt aaaattttga agataactca ttaatatcag ttagttgtgg gttattaaat
     2341 tacgcagtat tactttctgt aaggtgaatc caagtcgtcg gcgattcgtg tacgacttgc
     2401 tgacgactcg tcatagagtc atcgacgact cacttaagac tatcagtaac ttatgatgac
     2461 ttgactctag agttgaatcg tcattttggt gtttttttga cctttacgac tctagagtca
     2521 agtcacgagt cgaatcgccg gtgaattgtc tggtgagtcg tccggcgact tgccataacg
     2581 tgattgccgg ccgtcgacga acccgattcc tgatcgtgct gaaaaccggt tttccggctg
     2641 cgccggcaac gcttcacggc cacgcaataa attgcgtccc cgttcaccgt tttcggatag
     2701 gaagaacgcc ggaaacagga cttaccctgg ctgaatggcg tgaattttcg cgctatgcac
     2761 ttgcgtgcat actcatgcat gccgtaaaaa cagagcctgc gcgtttctgg cgggttttcg
     2821 ggtggtttgt tgcctgtttt accggtttcc cgtcagaaac gccctgaggc cgttttagtg
     2881 gtgcgtacaa ttaaaatgtt atgttaaatg tctgctgaat ggtttttagg atttttccta
     2941 acatatgata gaataaaaaa accatatgtt aatatattgt attttttcaa tgaaaatctc
     3001 aaagatatct gacgaacatt tttctaatta caacatgttg ccgaatggta atgaagaaat
     3061 tgaccttttt gacttgatta gagttttatg ggcagcaaaa ataaaaattg ttggggtgat
     3121 tctatttttt gcttgtattg gctttcttgt ttcttttgtt ttgccacaaa aatggactag
     3181 ttctgctgtg gttactcctg ctgagtctgt tcaatggagt gaattaaata aggagctttc
     3241 caagcttcat gttctgggag tggattttga tatcaatcgt gatagcgcat ttgctctgtt
     3301 tattaagaaa tttcagtccg ttaattcgtt gaatgagtat atgagatcat ctccttatgt
     3361 tatggagata attaaaaaga agaacattag cgctctcgaa ctgcatcgtg caattgttgg
     3421 gttgagtgaa aatatgaagt cggttgatga taatgccagt aagaaaaacg acaagtcatc
     3481 attgtatgta tcatggactc tgagttttac tgctccaaca agtaaagaag ctcacgatgt
     3541 gttgtctggg tatattaatt atgtttcttc ccttgttgta agggatttga tggaagatat
     3601 aagaaatgaa ctagaagtta aaactaatgt tgaaaaagaa attcttgcac tggatgagat
     3661 aaaaattaga aaccagctga atgcagatat tcgacgcctc aattattcac tggaggttgc
     3721 taatgcggct ggaataaaaa aacctgtata cagcaatggt cagattatga aggatgatcc
     3781 agattttcct gtggctctcg gttctgatgg tatagcaact aaattgaaca tcaaaaaatc
     3841 aatcaaggat gtttcggaat tgagtgggga gttgcgaaat cgtcaatatg ttgtgaatca
     3901 attggttgtg gcgaaagtgg gggatgttga ttttatgccg tttcagtatc aattgagccc
     3961 aacgttaccg gtcagaaagg atgggccaag taatgccata attgtaattc tttcttctgt
     4021 cattggaggg atgcttgcat gtggctatat acttttacgt catgccatgg aggcc
//
