LOCUS       NZ_CP081747             3211 bp    DNA     circular CON 02-NOV-2023
DEFINITION  Escherichia coli strain CX15 plasmid unnamed4, complete sequence.
ACCESSION   NZ_CP081747
VERSION     NZ_CP081747.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN18446046
            Assembly: GCF_019971075.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3211)
  AUTHORS   Yang,X., Tran,F. and Zhang,P.
  TITLE     Genetic characterization of Escherichia coli recovered from beef
            production environments, which harbor the locus of heat resistance
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3211)
  AUTHORS   Yang,X., Tran,F. and Zhang,P.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2021) Food Safety, Agriculture & Agri-Food Canada,
            6000 C & E Trail, Lacombe, Alberta T4L 1W1, Canada
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 26-JAN-2021
            Assembly Method                   :: flye v. 2.8.2
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 92x
            Sequencing Technology             :: Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/02/2023 04:08:11
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,175
            CDSs (total)                      :: 5,060
            Genes (coding)                    :: 4,801
            CDSs (with protein)               :: 4,801
            Genes (RNA)                       :: 115
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 259
            CDSs (without protein)            :: 259
            Pseudo Genes (ambiguous residues) :: 0 of 259
            Pseudo Genes (frameshifted)       :: 76 of 259
            Pseudo Genes (incomplete)         :: 171 of 259
            Pseudo Genes (internal stop)      :: 47 of 259
            Pseudo Genes (multiple problems)  :: 34 of 259
            Pseudo Genes (short protein)      :: 3 of 259
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP081747.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3211
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="CX15"
                     /db_xref="taxon:562"
                     /plasmid="unnamed4"
                     /geo_loc_name="Canada: Alberta"
                     /collection_date="2014/2015"
     gene            254..481
                     /locus_tag="J6O52_RS25675"
                     /old_locus_tag="J6O52_25595"
     CDS             254..481
                     /locus_tag="J6O52_RS25675"
                     /old_locus_tag="J6O52_25595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001046776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_206659768.1"
                     /translation="MFGFSPGELMNLCPDERLLFVRMISAMLRRSGGDAGAVMFEAYRH
                     IVSDTNQARRSCMLDLLESVRHDYVHGGYT"
                     /name="hypothetical protein"
     gene            complement(529..714)
                     /locus_tag="J6O52_RS25680"
                     /old_locus_tag="J6O52_25600"
     CDS             complement(529..714)
                     /locus_tag="J6O52_RS25680"
                     /old_locus_tag="J6O52_25600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_054626234.1"
                     /translation="MTKQESAALNMAKFIRAQSLLLEKLDVLDLDDEAADCERMHEQAE
                     ALYQKLSARFGIQDGE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            1260..1484
                     /locus_tag="J6O52_RS25685"
                     /old_locus_tag="J6O52_25605"
     CDS             1260..1484
                     /locus_tag="J6O52_RS25685"
                     /old_locus_tag="J6O52_25605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6290 family protein"
                     /protein_id="WP_000879771.1"
                     /translation="MLAIRLSDEIESRLDSLAKQTGRTKTFYAREAILAHLEDLEDYYL
                     SAETAARVRRGDEAVHSSEDVRKSLGLDD"
                     /name="DUF6290 family protein"
     gene            1468..1734
                     /locus_tag="J6O52_RS25690"
                     /old_locus_tag="J6O52_25610"
     CDS             1468..1734
                     /locus_tag="J6O52_RS25690"
                     /old_locus_tag="J6O52_25610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262326.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_000262326.1"
                     /translation="MVWTINYSDRALKSLRKMDKQNARRIVDFMDLRIAVAADPRQSGK
                     PLKGELGEFWRYRVGDYRILCEIRDDELVILAATIGHRREVYD"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            1844..2056
                     /locus_tag="J6O52_RS25695"
                     /old_locus_tag="J6O52_25615"
     CDS             1844..2056
                     /locus_tag="J6O52_RS25695"
                     /old_locus_tag="J6O52_25615"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_039509481.1"
                     /translation="MGDFERTYGAGADFDRIIDENNSEYFRDERQKAKNSKKEFSSFQD
                     ALVWAKANPGKTIVRSPDGVGFIEK"
                     /name="hypothetical protein"
     gene            complement(2477..2611)
                     /locus_tag="J6O52_RS25945"
                     /old_locus_tag="J6O52_25620"
     CDS             complement(2477..2611)
                     /locus_tag="J6O52_RS25945"
                     /old_locus_tag="J6O52_25620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_072035952.1"
                     /translation="MHEPPVQSDCCALSGNYRLESNPERHDKAPLAAATGNRFRGGES"
                     /name="replication initiation protein"
     Typing          complement(2503..2992)
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="96.13"
                     /coverage="81.0"
                     /evalue="0.0"
ORIGIN
        1 ttggcatctc tgaagtttag ccagtataca ctccgctagc gctacgtgac tggttcaggg
       61 ctgcgccccg acacccgcta aaagcactga cgcgcctgcg gcttgcccaa ccccgcgccg
      121 tccgggaaag aatttcccga tcgacccgac gtttctgttt acctgtgatg ttaacgtttg
      181 gatctttttc ccgggtttac ctattttctt ctaatgaagc gttcacagct gacgttaata
      241 gatgggtgct ttcatgtttg gtttttcacc aggagaactt atgaaccttt gccccgatga
      301 acgtctgctc tttgtgcgga tgatttccgc tatgcttcgc cgttcgggcg gtgatgcggg
      361 tgctgttatg tttgaggcgt atcgccatat tgtgtccgat accaatcaag ctcgtcgttc
      421 ctgcatgctc gacctgctcg aaagcgtcag gcatgactat gtacacggtg gatatacata
      481 gttgccctgg atacgctttt cgtctaagtg agcgttcttt tcgtctcttt attcgccgtc
      541 ctgtataccg aagcgtgcgc tcagcttctg ataaagcgct tctgcctgtt cgtgcattcg
      601 ctcacagtcg gctgcctcgt catcaagatc gagcacgtcg agtttttcga gcagcagcga
      661 ctgcgcccgg ataaattttg ccatgttcag tgcggctgat tcctgttttg tcatacggct
      721 ttttcccggt tttatggtga tcgcagactg aaatcttact tcattcactt gagtgcatag
      781 ccatgcaggt acgcagaaat catcagcgcg tttctgagcg gttttcaggg ggtaagatgt
      841 gttcttttgc aggtgaggtc gtagaaacgc cctgagagcc tctcaggcgg tgcacgcaac
      901 agatgttatg ttcaatcaga ctgatctgcg ggtacaaaaa aagccccgga gggcttttaa
      961 ctactgactt atttttttga ggatttgctg ggtttcaatt tgtcagtatt ccccggcgtt
     1021 tttttattgt cacgctggcg tttatcaggt tcccctgttg aatcggcaat tcgtttcgga
     1081 cccggcttaa gacccataat cgttatcctt tcaatagtta agaggaaagc cctcaagctg
     1141 gatatattga cagcgttacg gattatcaag aggggccagc aagattattt tttgttcttt
     1201 taggttgatt gcgatactat tgctatacat gtatatcaaa tggtgttcag gaggtttata
     1261 tgcttgctat ccggttatct gatgagattg agtcccgtct ggactcgctg gcgaagcaaa
     1321 ccggcagaac aaagacgttc tatgcgcggg aagcaatact ggcgcatctg gaagacctgg
     1381 aagattatta tctttcagca gaaactgccg cacgcgttcg ccgtggtgat gaagccgtgc
     1441 attcgtctga agacgtgagg aagtcacttg gtctggacga ttaactattc cgaccgggcg
     1501 ctcaaatcgt tacgcaagat ggacaaacag aacgcacgac ggattgtgga ttttatggat
     1561 ttacgcattg cagttgctgc cgatcctcgc cagtcaggga agccgctcaa aggtgagctg
     1621 ggcgagttct ggcgctatcg ggtgggagat tatcgcattc tgtgtgagat ccgggatgac
     1681 gagctggtta tccttgccgc caccattggc catcgccgcg aagtttacga ctgaagcccg
     1741 ctatgcgggc tttttttgat gctgacaacg gatgttatgg taaataagag tgtctgttgg
     1801 atattttaaa tctgtttttt tattaaattt atgggggtag gaaatggggg attttgagcg
     1861 tacctacgga gctggtgctg attttgatcg gataattgat gagaataaca gcgaatattt
     1921 tcgagatgag cgccaaaaag ctaaaaattc gaaaaaagaa ttctcttctt ttcaagatgc
     1981 tttggtttgg gcgaaagcta atcctggtaa aacgatagta agatctccag acggtgtggg
     2041 atttattgaa aaatgaaaga gtggattcac tgtaaaacgg cagtcatttt ttggacaaca
     2101 ctggctatat acgtactcat atgtttcaca gtgtcgtatg ttggagtcta tgttacgtac
     2161 attgcagggc ctattttgtt agttacagga attcttgcat ggcttacgaa acctagtagt
     2221 aattcaaata aatctaatat gtgactgggt aagacgaact tcatccaacc ataaaacccg
     2281 cttttttaaa cggatcttct tgagatcatt tagggagaat cgtaatctct tagctctgta
     2341 aacgaaaaaa ccacccggca aggtggcttt ttcgaaggtt cgcaagagtt gacgctcttt
     2401 tgaaccgcgg taactggctt ggaggagcac agtcacaaaa tagtcctttc agtgtagcct
     2461 tatcgcaccc gaaatttcaa gactctcctc ctctaaacct gttaccagtg gctgctgcca
     2521 gtggcgcttt gtcgtgtctt tccgggttgg actcaagacg atagttaccg gataaggcgc
     2581 agcagtcgga ctgaacgggg ggttcgtgca tacagtccag cttggagcga actgcctacc
     2641 cggaactgag tgtcaggcgt ggaatgagat aaacgcggcc ataacagcgg aatgacaccg
     2701 gtaaaccgaa aggcaggaac aggagagcgc acgagggagc caccaggggg aaacgcctgg
     2761 tatctttaag tcctgtcggg tttcgccacc actgatttga gcgtcagatt tcgtgatgct
     2821 tgtcaggggg gcggagccta tggaaaaacg gcttcgccgt ggccttcttg cttccctgtt
     2881 aagcttcttc ctggaagatg ccaggaaatt tccgccccgc cagtaagccg gtaccgctcg
     2941 ccgcagccga acgaccgagc ggagcgagtc agtgagcgag gaagcggaat atctccgggc
     3001 ttatctttgg caccgtacgc gccatagctg cattttttaa tacgatgcag gatagggcaa
     3061 acgccgcaaa atgacgtcat ccgacatcat tcctccgcgt tcgtccagtg acctacagtc
     3121 ggctctgggg tgtcggggct gtgccctgac caggcgtttt ggagtatcgc caccgtggtg
     3181 gcggtgctac aacacatatc ctgtcccttc c
//
