LOCUS       NZ_CP081746             4077 bp    DNA     circular CON 02-NOV-2023
DEFINITION  Escherichia coli strain CX15 plasmid unnamed3, complete sequence.
ACCESSION   NZ_CP081746
VERSION     NZ_CP081746.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN18446046
            Assembly: GCF_019971075.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4077)
  AUTHORS   Yang,X., Tran,F. and Zhang,P.
  TITLE     Genetic characterization of Escherichia coli recovered from beef
            production environments, which harbor the locus of heat resistance
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4077)
  AUTHORS   Yang,X., Tran,F. and Zhang,P.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2021) Food Safety, Agriculture & Agri-Food Canada,
            6000 C & E Trail, Lacombe, Alberta T4L 1W1, Canada
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 26-JAN-2021
            Assembly Method                   :: flye v. 2.8.2
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 92x
            Sequencing Technology             :: Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/02/2023 04:08:11
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,175
            CDSs (total)                      :: 5,060
            Genes (coding)                    :: 4,801
            CDSs (with protein)               :: 4,801
            Genes (RNA)                       :: 115
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 259
            CDSs (without protein)            :: 259
            Pseudo Genes (ambiguous residues) :: 0 of 259
            Pseudo Genes (frameshifted)       :: 76 of 259
            Pseudo Genes (incomplete)         :: 171 of 259
            Pseudo Genes (internal stop)      :: 47 of 259
            Pseudo Genes (multiple problems)  :: 34 of 259
            Pseudo Genes (short protein)      :: 3 of 259
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP081746.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4077
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="CX15"
                     /db_xref="taxon:562"
                     /plasmid="unnamed3"
                     /geo_loc_name="Canada: Alberta"
                     /collection_date="2014/2015"
     gene            join(2979..4077,1..8)
                     /gene="wzz(fepE)"
                     /locus_tag="J6O52_RS25655"
                     /old_locus_tag="J6O52_25575"
     CDS             join(2979..4077,1..8)
                     /gene="wzz(fepE)"
                     /locus_tag="J6O52_RS25655"
                     /old_locus_tag="J6O52_25575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004030247.1"
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_process="GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen length regulator Wzz(fepE)"
                     /protein_id="WP_223617417.1"
                     /translation="MFSMKISKISDEHFSNYNMLPNGNEEIDLFDLIRVLWAAKIKIVG
                     VILFFACIGFLVSFVLPQKWTSSAVVTPAESVQWSELNKELSKLHVLGVDFDINRDSAF
                     ALFIKKFQSVNSLNEYMRSSPYVMEIIKKKNISALELHRAIVGLSENMKSVDDNASKKN
                     DKSSLYVSWTLSFTAPTSKEAHDVLSGYINYVSSLVVRDLMEDIRNELEVKTNVEKEIL
                     ALDEIKIRNQLNADIRRLNYSLEVANAAGIKKPVYSNGQIMKDDPDFPVALGSDGIATK
                     LNIKKSIKDVSELSGELRNRQYVVNQLVVAKVGDVDFMPFQYQLSPTLPVRKDGPSNAI
                     IVILSSVIGGMLACGYILLRHAMEARKR"
                     /name="wzz(fepE)"
     gene            959..1108
                     /locus_tag="J6O52_RS25660"
                     /old_locus_tag="J6O52_25580"
     CDS             959..1108
                     /locus_tag="J6O52_RS25660"
                     /old_locus_tag="J6O52_25580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017880354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001515742.1"
                     /translation="MPSEYDEQPDATDALFPGEYDLFDLMAHVTEENLHAPVDSGPPVG
                     REAL"
                     /name="hypothetical protein"
     gene            1577..1768
                     /locus_tag="J6O52_RS25665"
                     /old_locus_tag="J6O52_25585"
     CDS             1577..1768
                     /locus_tag="J6O52_RS25665"
                     /old_locus_tag="J6O52_25585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000166329.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELDLDVEADLCETLHDDA
                     EHLFRTLSSRLDALQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            1768..2169
                     /locus_tag="J6O52_RS25670"
                     /old_locus_tag="J6O52_25590"
     CDS             1768..2169
                     /locus_tag="J6O52_RS25670"
                     /old_locus_tag="J6O52_25590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310048.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_000135006.1"
                     /translation="MARVSISEAARLTGKSRTTLHRLIKAGDLSTCSGERNAKMVDTSE
                     LLRVFGPFEQPKGEHHSGQVSEQRDTASADQSEQVTRHLKQEIEHLNTLLLSKDSHIDS
                     LKQAMLLLENHQAKLTESPAPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     Typing          752..966
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="84.091"
                     /coverage="100.0"
                     /evalue="3.48e-51"
     Typing          complement(243..771)
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="84.545"
                     /coverage="89.0"
                     /evalue="1.2e-144"
ORIGIN
        1 aaagatgagt gtgattgagt aaaagttgtg ctgatgcatc attttataag caacttttat
       61 aaaaagttca aaggatcttc ttgagatcat tcccttacag ccgtagtctt ctactctaga
      121 aaacgaaaaa accacctggg gaggtggttt ttcgaaggtt cagtaagttg gggaacttct
      181 gaaccgtggt aacagtcttg tgcgagacat gtcaccaaat ctgtccttct agtgtagcct
      241 cagtaaggct gccacttcaa gaactctcaa tacatctctc gcacatcctg tttaccagtg
      301 gccgctgcca atggcgttaa gtcgtgtctt accgggttgg actcaagacg atagttaccg
      361 gataaggcgc agcggtcggg ctgaacgggg ggttcttgca tacagcccag cttggagcga
      421 actgtctaaa cggaacgggg cgtggtgatt taggtaaagc ttccaccact acacggatgc
      481 cgcaggacgg gaacaggaga gcgcaagagg gagccaccag ggggaaacgc ctggtatctt
      541 tatagtcctg tcgggtttcg ccaccactga tttgagcgtc agatttcgtg atgcttgtca
      601 ggggggcgga gcctatggaa aaacggcttt gccgcggcct tatcgtatcc ctgttaagtt
      661 tcttcctggc atccaccagg aaataaattt ccgccccatt cgtaagcctg caccgctcgc
      721 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaata tcacctgaac
      781 cagcatcttc tcctgacgcc ggtaagcctc atttcactcc tgccacatga agcactttgg
      841 caaatcacca cttcggttta aaatatttag ccagtatgca ctccgctagc gctacgtgac
      901 tggttcaggg ctgcgccccg aaacccgcta acacccctca gctactggat atcacaccat
      961 gccatctgag tatgatgaac aaccggatgc gactgatgct ctgtttcccg gcgaatatga
     1021 cctttttgac ttaatggctc atgttactga agagaacctg cacgccccgg tggattctgg
     1081 cccgcctgtc ggccgggaag cgttatagct ttttcgctgg ccagcgtaca tgtaatacgc
     1141 tggtattaca tgttatttgt ttattttcat gttgttacag atgattgtaa tacgttgcgt
     1201 attacgctaa tacacgaaat aactccattt ttttcattta attcatgcag ttgcgataaa
     1261 aatcgcggct gcgttaggtg tatgaccatt taaggggcaa aaaattatca ttctggctgg
     1321 gtgatctgcg caggttaagg atcatcggcg acgaaaaagc gtctttaact gcttcgttca
     1381 tgcaagcatg aacggattat ttttccagac tacaggaaga ctacaaaagt tgatgattta
     1441 atcctgcgtt ttagtgcgat gactacagat aaactacaaa ataactacag gataacctca
     1501 gatccgttct ctgaaccatt cgtacatccg acccccttac cggtttatac tgagcactga
     1561 tgctctgagg gcccttatga cgaaacaaca gaaaacagcg ctgaacatgg ctaaattcat
     1621 ccagaatcag tctctgctcc tgctggagaa gcttaacgag cttgatctcg atgttgaggc
     1681 ggacctttgt gagacactcc acgatgatgc ggagcatctt ttccgtacac tgtcctcgag
     1741 attggatgca ttacaggatg gtaactgatg gccagagtat ctattagtga ggctgcccga
     1801 ctgacgggta agagtagaac aacactgcac aggttaatta aagcaggtga cctgtccacc
     1861 tgtagcggtg aacgcaatgc aaaaatggta gatacttcag agcttttacg tgtctttggt
     1921 ccctttgaac aaccgaaggg tgaacaccat tctggacagg tctctgaaca gcgtgataca
     1981 gcctctgctg accagagtga acaggtgact cgtcatctga aacaggagat tgaacacctg
     2041 aacaccttac ttttgtccaa ggattcgcac attgacagcc tgaaacaggc tatgctcctt
     2101 cttgaaaatc accaggcaaa attaactgaa tccccggcgc catggtggaa attctggaaa
     2161 aaatcctgac cccgtcatgc ttcagttttc ctctccggcc atcccgggga gttcctgaac
     2221 cttcccaaaa atcgtcagat ctctgaacgc tcctcaccac taaaaatacc cacaccctac
     2281 gcaaaattaa aattttgaag ataactcatt aatatcagtt agttgtgggt tattaaatta
     2341 cgcagtatta ctttctgtaa ggtgaatcca agtcgtcggc gattcgtgta cgacttgctg
     2401 acgactcgtc atagagtcat cgacgactca cttaagacta tcagtaactt atgatgactt
     2461 gactctagag ttgaatcgtc attttggtgt ttttttgacc tttacgactc tagagtcaag
     2521 tcacgagtcg aatcgccggt gaattgtctg gtgagtcgtc cggcgacttg ccataacgtg
     2581 attgccggcc gtcgacgaac ccgattcctg atcgtgctga aaaccggttt tccggctgcg
     2641 ccggcaacgc ttcacggcca cgcaataaat tgcgtccccg ttcaccgttt tcggatagga
     2701 agaacgccgg aaacaggact taccctggct gaatggcgtg aattttcgcg ctatgcactt
     2761 gcgtgcatac tcatgcatgc cgtaaaaaca gagcctgcgc gtttctggcg ggttttcggg
     2821 tggtttgttg cctgttttac cggtttcccg tcagaaacgc cctgaggccg ttttagtggt
     2881 gcgtacaatt aaaatgttat gttaaatgtc tgctgaatgg tttttaggat ttttcctaac
     2941 atatgataga ataaaaaaac catatgttaa tatattgtat tttttcaatg aaaatctcaa
     3001 agatatctga cgaacatttt tctaattaca acatgttgcc gaatggtaat gaagaaattg
     3061 acctttttga cttgattaga gttttatggg cagcaaaaat aaaaattgtt ggggtgattc
     3121 tattttttgc ttgtattggc tttcttgttt cttttgtttt gccacaaaaa tggactagtt
     3181 ctgctgtggt tactcctgct gagtctgttc aatggagtga attaaataag gagctttcca
     3241 agcttcatgt tctgggagtg gattttgata tcaatcgtga tagcgcattt gctctgttta
     3301 ttaagaaatt tcagtccgtt aattcgttga atgagtatat gagatcatct ccttatgtta
     3361 tggagataat taaaaagaag aacattagcg ctctcgaact gcatcgtgca attgttgggt
     3421 tgagtgaaaa tatgaagtcg gttgatgata atgccagtaa gaaaaacgac aagtcatcat
     3481 tgtatgtatc atggactctg agttttactg ctccaacaag taaagaagct cacgatgtgt
     3541 tgtctgggta tattaattat gtttcttccc ttgttgtaag ggatttgatg gaagatataa
     3601 gaaatgaact agaagttaaa actaatgttg aaaaagaaat tcttgcactg gatgagataa
     3661 aaattagaaa ccagctgaat gcagatattc gacgcctcaa ttattcactg gaggttgcta
     3721 atgcggctgg aataaaaaaa cctgtataca gcaatggtca gattatgaag gatgatccag
     3781 attttcctgt ggctctcggt tctgatggta tagcaactaa attgaacatc aaaaaatcaa
     3841 tcaaggatgt ttcggaattg agtggggagt tgcgaaatcg tcaatatgtt gtgaatcaat
     3901 tggttgtggc gaaagtgggg gatgttgatt ttatgccgtt tcagtatcaa ttgagcccaa
     3961 cgttaccggt cagaaaggat gggccaagta atgccataat tgtaattctt tcttctgtca
     4021 ttggagggat gcttgcatgt ggctatatac ttttacgtca tgccatggag gccagaa
//
