LOCUS       NZ_CP081723             3206 bp    DNA     circular CON 02-NOV-2023
DEFINITION  Escherichia coli strain CX05 plasmid unnamed4, complete sequence.
ACCESSION   NZ_CP081723
VERSION     NZ_CP081723.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN18446039
            Assembly: GCF_019971035.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3206)
  AUTHORS   Yang,X., Tran,F. and Zhang,P.
  TITLE     Genetic characterization of Escherichia coli recovered from beef
            production environments, which harbor the locus of heat resistance
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3206)
  AUTHORS   Yang,X., Tran,F. and Zhang,P.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2021) Food Safety, Agriculture & Agri-Food Canada,
            6000 C & E Trail, Lacombe, Alberta T4L 1W1, Canada
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 26-JAN-2021
            Assembly Method                   :: flye v. 2.8.2
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 138x
            Sequencing Technology             :: Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/02/2023 04:01:54
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,033
            Genes (coding)                    :: 4,779
            CDSs (with protein)               :: 4,779
            Genes (RNA)                       :: 115
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 71 of 254
            Pseudo Genes (incomplete)         :: 170 of 254
            Pseudo Genes (internal stop)      :: 47 of 254
            Pseudo Genes (multiple problems)  :: 33 of 254
            Pseudo Genes (short protein)      :: 3 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP081723.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3206
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="CX05"
                     /db_xref="taxon:562"
                     /plasmid="unnamed4"
                     /geo_loc_name="Canada: Alberta"
                     /collection_date="2014/2015"
     gene            complement(189..401)
                     /locus_tag="J6O38_RS25550"
                     /old_locus_tag="J6O38_25455"
     CDS             complement(189..401)
                     /locus_tag="J6O38_RS25550"
                     /old_locus_tag="J6O38_25455"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_039509481.1"
                     /translation="MGDFERTYGAGADFDRIIDENNSEYFRDERQKAKNSKKEFSSFQD
                     ALVWAKANPGKTIVRSPDGVGFIEK"
                     /name="hypothetical protein"
     gene            complement(511..777)
                     /locus_tag="J6O38_RS25555"
                     /old_locus_tag="J6O38_25460"
     CDS             complement(511..777)
                     /locus_tag="J6O38_RS25555"
                     /old_locus_tag="J6O38_25460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262326.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_000262326.1"
                     /translation="MVWTINYSDRALKSLRKMDKQNARRIVDFMDLRIAVAADPRQSGK
                     PLKGELGEFWRYRVGDYRILCEIRDDELVILAATIGHRREVYD"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(761..985)
                     /locus_tag="J6O38_RS25560"
                     /old_locus_tag="J6O38_25465"
     CDS             complement(761..985)
                     /locus_tag="J6O38_RS25560"
                     /old_locus_tag="J6O38_25465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6290 family protein"
                     /protein_id="WP_000879771.1"
                     /translation="MLAIRLSDEIESRLDSLAKQTGRTKTFYAREAILAHLEDLEDYYL
                     SAETAARVRRGDEAVHSSEDVRKSLGLDD"
                     /name="DUF6290 family protein"
     gene            1531..1716
                     /locus_tag="J6O38_RS25565"
                     /old_locus_tag="J6O38_25470"
     CDS             1531..1716
                     /locus_tag="J6O38_RS25565"
                     /old_locus_tag="J6O38_25470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_054626234.1"
                     /translation="MTKQESAALNMAKFIRAQSLLLEKLDVLDLDDEAADCERMHEQAE
                     ALYQKLSARFGIQDGE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(1764..1991)
                     /locus_tag="J6O38_RS25570"
                     /old_locus_tag="J6O38_25475"
     CDS             complement(1764..1991)
                     /locus_tag="J6O38_RS25570"
                     /old_locus_tag="J6O38_25475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001046776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_206659768.1"
                     /translation="MFGFSPGELMNLCPDERLLFVRMISAMLRRSGGDAGAVMFEAYRH
                     IVSDTNQARRSCMLDLLESVRHDYVHGGYT"
                     /name="hypothetical protein"
     gene            2845..2979
                     /locus_tag="J6O38_RS25805"
                     /old_locus_tag="J6O38_25480"
     CDS             2845..2979
                     /locus_tag="J6O38_RS25805"
                     /old_locus_tag="J6O38_25480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_072035952.1"
                     /translation="MHEPPVQSDCCALSGNYRLESNPERHDKAPLAAATGNRFRGGES"
                     /name="replication initiation protein"
     Typing          2465..2954
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="96.13"
                     /coverage="81.0"
                     /evalue="0.0"
ORIGIN
        1 tcacatatta gatttatttg aattactact aggtttcgta agccatgcaa gaattcctgt
       61 aactaacaaa ataggccctg caatgtacgt aacatagact ccaacatacg acactgtgaa
      121 acatatgagt acgtatatag ccagtgttgt ccaaaaaatg actgccgttt tacagtgaat
      181 ccactctttc atttttcaat aaatcccaca ccgtctggag atcttactat cgttttacca
      241 ggattagctt tcgcccaaac caaagcatct tgaaaagaag agaattcttt tttcgaattt
      301 ttagcttttt ggcgctcatc tcgaaaatat tcgctgttat tctcatcaat tatccgatca
      361 aaatcagcac cagctccgta ggtacgctca aaatccccca tttcctaccc ccataaattt
      421 aataaaaaaa cagatttaaa atatccaaca gacactctta tttaccataa catccgttgt
      481 cagcatcaaa aaaagcccgc atagcgggct tcagtcgtaa acttcgcggc gatggccaat
      541 ggtggcggca aggataacca gctcgtcatc ccggatctca cacagaatgc gataatctcc
      601 cacccgatag cgccagaact cgcccagctc acctttgagc ggcttccctg actggcgagg
      661 atcggcagca actgcaatgc gtaaatccat aaaatccaca atccgtcgtg cgttctgttt
      721 gtccatcttg cgtaacgatt tgagcgcccg gtcggaatag ttaatcgtcc agaccaagtg
      781 acttcctcac gtcttcagac gaatgcacgg cttcatcacc acggcgaacg cgtgcggcag
      841 tttctgctga aagataataa tcttccaggt cttccagatg cgccagtatt gcttcccgcg
      901 catagaacgt ctttgttctg ccggtttgct tcgccagcga gtccagacgg gactcaatct
      961 catcagataa ccggatagca agcatataaa cctcctgaac accatttgat atacatgtat
     1021 agcaatagta tcgcaatcaa cctaaaagaa caaaaaataa tcttgctggc ccctcttgat
     1081 aatccgtaac gctgtcaata tatccagctt gagggctttc ctcttaacta ttgaaaggat
     1141 aacgattatg ggtcttaagc cgggtccgaa acgaattgcc gattcaacag gggaacctga
     1201 taaacgccag cgtgacaata aaaaaacgcc ggggaatact gacaaattga aacccagcaa
     1261 atcctcaaaa aaataagtca gtagttaaaa gccctccggg gctttttttg tacccgcaga
     1321 tcagtctgat tgaacataac atctgttgcg tgcaccgcct gagaggctct cagggcgttt
     1381 ctacgacctc acctgcaaaa gaacacatct taccccctga aaaccgctca gaaacgcgct
     1441 gatgatttct gcgtacctgc atggctatgc actcaagtga atgaagtaag atttcagtct
     1501 gcgatcacca taaaaccggg aaaaagccgt atgacaaaac aggaatcagc cgcactgaac
     1561 atggcaaaat ttatccgggc gcagtcgctg ctgctcgaaa aactcgacgt gctcgatctt
     1621 gatgacgagg cagccgactg tgagcgaatg cacgaacagg cagaagcgct ttatcagaag
     1681 ctgagcgcac gcttcggtat acaggacggc gaataaagag acgaaaagaa cgctcactta
     1741 gacgaaaagc gtatccaggg caactatgta tatccaccgt gtacatagtc atgcctgacg
     1801 ctttcgagca ggtcgagcat gcaggaacga cgagcttgat tggtatcgga cacaatatgg
     1861 cgatacgcct caaacataac agcacccgca tcaccgcccg aacggcgaag catagcggaa
     1921 atcatccgca caaagagcag acgttcatcg gggcaaaggt tcataagttc tcctggtgaa
     1981 aaaccaaaca tgaaagcacc catctattaa cgtcagctgt gaacgcttca ttagaagaaa
     2041 ataggtaaac ccgggaaaaa gatccaaacg ttaacatcac aggtaaacag aaacgtcggg
     2101 tcgatcggga aattctttcc cggacggcgc ggggttgggc aagccgcagg cgcgtcagtg
     2161 cttttagcgg gtgtcggggc gcagccctga accagtcacg tagcgctagc ggagtgtata
     2221 ctggctaaac ttcagagatg ccaaggaagg gacaggatat gtgttgtagc accgccacca
     2281 cggtggcgat actccaaaac gcctggtcag ggcacagccc cgacacccca gagccgactg
     2341 taggtcactg gacgaacgcg gaggaatgat gtcggatgac gtcattttgc ggcgtttgcc
     2401 ctatcctgca tcgtattaaa aaatgcagct atggcgcgta cggtgccaaa gataagcccg
     2461 gagatattcc gcttcctcgc tcactgactc gctccgctcg gtcgttcggc tgcggcgagc
     2521 ggtaccggct tactggcggg gcggaaattt cctggcatct tccaggaaga agcttaacag
     2581 ggaagcaaga aggccacggc gaagccgttt ttccataggc tccgcccccc tgacaagcat
     2641 cacgaaatct gacgctcaaa tcagtggtgg cgaaacccga caggacttaa agataccagg
     2701 cgtttccccc tggtggctcc ctcgtgcgct ctcctgttcc tgcctttcgg tttaccggtg
     2761 tcattccgct gttatggccg cgtttatctc attccacgcc tgacactcag ttccgggtag
     2821 gcagttcgct ccaagctgga ctgtatgcac gaaccccccg ttcagtccga ctgctgcgcc
     2881 ttatccggta actatcgtct tgagtccaac ccggaaagac acgacaaagc gccactggca
     2941 gcagccactg gtaacaggtt tagaggagga gagtcttgaa atttcgggtg cgataaggct
     3001 acactgaaag gactattttg tgactgtgct cctccaagcc agttaccgcg gttcaaaaga
     3061 gcgtcaactc ttgcgaacct tcgaaaaagc caccttgccg ggtggttttt tcgtttacag
     3121 agctaagaga ttacgattct ccctaaatga tctcaagaag atccgtttaa aaaagcgggt
     3181 tttatggttg gatgaagttc gtctta
//
