LOCUS       NZ_CP081710             2762 bp    DNA     circular CON 02-NOV-2023
DEFINITION  Escherichia coli strain AH04 plasmid unnamed4, complete sequence.
ACCESSION   NZ_CP081710
VERSION     NZ_CP081710.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN18446036
            Assembly: GCF_019970975.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2762)
  AUTHORS   Yang,X., Tran,F. and Zhang,P.
  TITLE     Genetic characterization of Escherichia coli recovered from beef
            production environments, which harbor the locus of heat resistance
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2762)
  AUTHORS   Yang,X., Tran,F. and Zhang,P.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2021) Food Safety, Agriculture & Agri-Food Canada,
            6000 C & E Trail, Lacombe, Alberta T4L 1W1, Canada
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 26-JAN-2021
            Assembly Method                   :: flye v. 2.8.2
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 65x
            Sequencing Technology             :: Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/02/2023 04:01:37
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,079
            CDSs (total)                      :: 4,953
            Genes (coding)                    :: 4,655
            CDSs (with protein)               :: 4,655
            Genes (RNA)                       :: 126
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 90
            ncRNAs                            :: 14
            Pseudo Genes (total)              :: 298
            CDSs (without protein)            :: 298
            Pseudo Genes (ambiguous residues) :: 0 of 298
            Pseudo Genes (frameshifted)       :: 72 of 298
            Pseudo Genes (incomplete)         :: 220 of 298
            Pseudo Genes (internal stop)      :: 43 of 298
            Pseudo Genes (multiple problems)  :: 35 of 298
            Pseudo Genes (short protein)      :: 3 of 298
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP081710.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2762
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="AH04"
                     /db_xref="taxon:562"
                     /plasmid="unnamed4"
                     /geo_loc_name="Canada: Alberta"
                     /collection_date="2013"
     gene            complement(166..771)
                     /locus_tag="J6O21_RS25225"
                     /old_locus_tag="J6O21_25075"
     CDS             complement(166..771)
                     /locus_tag="J6O21_RS25225"
                     /old_locus_tag="J6O21_25075"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_054623596.1"
                     /translation="MSFIKSLNKKNQTDQIEVTINVRLTQEEDHLLTLATQQSGYKRNE
                     LFYLLAKEYIFDDESLKEMAEESGAEFSEGLYQNCYVVNTNRVHHEEDDKFMISEGVAA
                     TFEDGYKEKMEQVKKGDLVFLYRSGEGIVAHGIASGDVLKKDHNGVPDKTYYQKLSGFV
                     RYEKPLTASEIQRILGRNVVFMHTLFSIRDGYKLLKKD"
                     /name="hypothetical protein"
     gene            973..1989
                     /gene="mobV"
                     /locus_tag="J6O21_RS25230"
                     /old_locus_tag="J6O21_25080"
     CDS             973..1989
                     /gene="mobV"
                     /locus_tag="J6O21_RS25230"
                     /old_locus_tag="J6O21_25080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000024818.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobV family relaxase"
                     /protein_id="WP_136655212.1"
                     /translation="MRSPDWLDGRQNEGVSMANYAIMRCKKLTGMGSVASALQHCYRER
                     ETPNADAERTPENYCSVSQSADEAMGKLRELLPEKRRKDAVLAVEYVMTASPEWWKEAT
                     PRQQAEFFARSEQWLEKKYGKDRVVAAVVHRDEATPHLSAFVVPLTQDGRLSAKEFIGG
                     RSKMRDDQSTYAESVKKLGLERGIEGSRATHQTVQHYYESINRGTRSQVSISPEALEPR
                     VLRKGIFTKDVEDQAAIAKRLSHAVNDGFAGTIAMASQSAQNAKRARELQKTMDSQQKR
                     LQSVTEPFKGLSREQMTEILMMAQRFKQQNQEKEKQQRVEREKQRQMRSRGMGGMER"
                     /name="mobV"
     gene            complement(2053..2379)
                     /locus_tag="J6O21_RS25235"
                     /old_locus_tag="J6O21_25085"
     CDS             complement(2053..2379)
                     /locus_tag="J6O21_RS25235"
                     /old_locus_tag="J6O21_25085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001070513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_223571429.1"
                     /translation="MNQAALITIANELNHDQMRVLMALLAELDYENYIQVAQMDIAEAL
                     TMQKTNVSRAVKNLIDFGIILEGPKIGRSKTYRLNPQFGWKGTVSNHKKALKNGLSVIQ
                     GGRT"
                     /name="helix-turn-helix domain-containing protein"
     Typing          974..1986
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="97.337"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 tgagtctgcg gcgagcggtt cttgattttt taaaacgata ttcggagctt tatttcgtca
       61 agggcgagcg tagcgagttc atttaccctt gacgatgcac tgtaaaaaac acttccggaa
      121 ggtggttggt gtcacaaagt gatgccaacc acctgtagac tgggtttaat cttttttaag
      181 cagtttatag ccatctctaa tgctaaatag cgtgtgcata aacacaacgt ttctccccag
      241 tattctctgg atctcagacg cggttagtgg tttttcgtac ctaacaaagc ctgatagctt
      301 ctggtagtag gtcttgtctg gcacgccatt atgatctttc tttaaaacat caccactggc
      361 gataccatgt gcaacaattc cttcgccgga gcgataaaga aagactaaat ctcctttttt
      421 aacctgctcc attttttcct tataaccatc ttcaaatgtt gccgctacgc cctctgaaat
      481 catgaactta tcatcttctt catgatgaac tctgttagtg ttaaccacat aacaattttg
      541 gtataagcct tctgaaaact cagcgccaga ctcctcagcc atttccttta agctttcatc
      601 atcaaagatg tattcctttg ctaggaggta gaatagctcg ttccttttat aaccagattg
      661 ctgggttgct aatgtcagta aatggtcctc ttcttgggtt aacctaacgt taatcgtcac
      721 ttctatttgg tctgtttgat tttttttgtt tagcgatttt atgaatgaca tcttactttc
      781 ctcaccagag ttaatgtact aatcaatgat agtgaatcac tacattgaaa cagaagcatt
      841 gatcacatct ttgatttaag caatgacttt atgtcatata tttttattaa gttgaattta
      901 acgaacgatt ttgcgtagca aagtctagtg agtacactat catcctgata gtggcccgcc
      961 ggaggcatcg caatgcgatc ccccgactgg ctggatggac gccagaacga gggggtaagc
     1021 atggcgaatt acgcgataat gcgctgcaaa aagctgacgg gaatgggcag tgtggccagc
     1081 gctttgcagc actgctacag ggagcgtgag acgccgaatg ctgacgctga acggacacca
     1141 gaaaactact gctctgtgtc tcagtcggca gatgaggcga tgggaaagct gcgggagctg
     1201 ttgccggaaa aacgccgtaa agatgcggta ctggctgttg aatacgttat gacggcaagc
     1261 cctgagtggt ggaaagaagc gacaccgcgg cagcaggcgg agtttttcgc ccgctctgag
     1321 caatggctgg agaagaaata cgggaaggat cgtgttgtgg cggcggtggt tcaccgggac
     1381 gaggccaccc cgcacttgtc agcgttcgtg gtgccattga cgcaggatgg acggctgtcc
     1441 gcgaaagagt ttattggcgg gcggtcaaag atgcgtgacg atcagagcac ctacgctgaa
     1501 tcagtaaaaa aactgggtct tgagcgcggc atcgagggaa gccgcgccac gcatcagaca
     1561 gttcagcact actacgaatc gataaaccgt ggcacccgta gccaggtatc gatctcgccg
     1621 gaggcgctgg agccgcgtgt attgcggaag ggcattttca ccaaagacgt ggaagatcag
     1681 gcagccatag ccaaacgtct aagccacgca gtgaacgatg gctttgcagg aaccatcgct
     1741 atggcctctc agagcgccca gaatgcaaaa agagcgcgcg agctacagaa gactatggat
     1801 tcgcagcaga aacgcctaca gagcgttaca gagcctttta aggggctttc cagagagcag
     1861 atgacagaaa tcctgatgat ggctcagcgg tttaagcagc aaaaccagga gaaggagaaa
     1921 cagcagcgtg ttgaacgtga gaaacagcga cagatgcgat cgcgtggcat gggcggtatg
     1981 gagcgttagt tttgatgcga tgcaggatag ggctaacgcc gcaaaatgac gccatttgcg
     2041 gcattccaga gatcaggttc tgccgccctg tataacgctg aggccgtttt tcagtgcttt
     2101 tttgtggttg ctgacagtac ctttccagcc gaactgggga tttaggcggt acgttttgct
     2161 ccgcccaatc ttcggccctt ccagtatgat cccgaaatca atcaggtttt ttaccgcacg
     2221 actgacattt gttttttgca tagtcagcgc ttccgcaata tccatctgcg caacttggat
     2281 gtagttctcg taatccagtt ctgcgagcaa ggccatcagc acacgcatct ggtcatggtt
     2341 tagctcattg gcaatggtaa ttagcgcggc ttggttcata gtaaagtgcc tctggaatga
     2401 tgacttttgt ttaggacgaa tcactgctac aaaaccacct aattcctccc ctgtagtttg
     2461 gtctacctgc gtgattctcc gcatctaaaa atctccattc aggaaaaaat agttatctct
     2521 caagataact aagttatctc tagagataac tattgtcaaa aactttccgc ttgtggcgcg
     2581 gcttcccagc catccccttc tgtatcttat tactctccac aatcacacat cggcaaaatc
     2641 aggcgttttt ctgcccgttc ggtgcgaacg aatttctatg tacgggaagt ggaaattccg
     2701 cttcctcgct cattcgctca ctacgttcgg tcatgagtct gcggcgagcg gttcttgatt
     2761 tt
//
