LOCUS       NZ_CP081709             7467 bp    DNA     circular CON 02-NOV-2023
DEFINITION  Escherichia coli strain AH04 plasmid unnamed3, complete sequence.
ACCESSION   NZ_CP081709
VERSION     NZ_CP081709.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN18446036
            Assembly: GCF_019970975.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7467)
  AUTHORS   Yang,X., Tran,F. and Zhang,P.
  TITLE     Genetic characterization of Escherichia coli recovered from beef
            production environments, which harbor the locus of heat resistance
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7467)
  AUTHORS   Yang,X., Tran,F. and Zhang,P.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2021) Food Safety, Agriculture & Agri-Food Canada,
            6000 C & E Trail, Lacombe, Alberta T4L 1W1, Canada
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 26-JAN-2021
            Assembly Method                   :: flye v. 2.8.2
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 65x
            Sequencing Technology             :: Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/02/2023 04:01:37
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,079
            CDSs (total)                      :: 4,953
            Genes (coding)                    :: 4,655
            CDSs (with protein)               :: 4,655
            Genes (RNA)                       :: 126
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 90
            ncRNAs                            :: 14
            Pseudo Genes (total)              :: 298
            CDSs (without protein)            :: 298
            Pseudo Genes (ambiguous residues) :: 0 of 298
            Pseudo Genes (frameshifted)       :: 72 of 298
            Pseudo Genes (incomplete)         :: 220 of 298
            Pseudo Genes (internal stop)      :: 43 of 298
            Pseudo Genes (multiple problems)  :: 35 of 298
            Pseudo Genes (short protein)      :: 3 of 298
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP081709.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..7467
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="AH04"
                     /db_xref="taxon:562"
                     /plasmid="unnamed3"
                     /geo_loc_name="Canada: Alberta"
                     /collection_date="2013"
     gene            complement(<36..350)
                     /locus_tag="J6O21_RS25185"
                     /old_locus_tag="J6O21_25035"
                     /pseudo=""
     CDS             complement(<36..350)
                     /locus_tag="J6O21_RS25185"
                     /old_locus_tag="J6O21_25035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228930.1"
                     /GO_function="GO:0004371 - glycerone kinase activity
                     [Evidence IEA]"
                     /GO_process="GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /name="dihydroxyacetone kinase subunit DhaK"
     gene            909..2005
                     /locus_tag="J6O21_RS25190"
                     /old_locus_tag="J6O21_25040"
                     /pseudo=""
     CDS             909..2005
                     /locus_tag="J6O21_RS25190"
                     /old_locus_tag="J6O21_25040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228925.1"
                     /GO_function="GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]; GO:0008888 - glycerol dehydrogenase [NAD+]
                     activity [Evidence IEA]; GO:0008270 - zinc ion binding
                     [Evidence IEA]"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol dehydrogenase"
                     /name="glycerol dehydrogenase"
     gene            2107..4031
                     /gene="dhaR"
                     /locus_tag="J6O21_RS25195"
                     /old_locus_tag="J6O21_25045"
                     /pseudo=""
     CDS             2107..4031
                     /gene="dhaR"
                     /locus_tag="J6O21_RS25195"
                     /old_locus_tag="J6O21_25045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228924.1"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA];
                     GO:0043565 - sequence-specific DNA binding [Evidence IEA];
                     GO:0008134 - transcription factor binding [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase operon transcriptional
                     regulator DhaR"
                     /name="dhaR"
     gene            complement(4009..4540)
                     /locus_tag="J6O21_RS25200"
                     /old_locus_tag="J6O21_25050"
                     /pseudo=""
     CDS             complement(4009..4540)
                     /locus_tag="J6O21_RS25200"
                     /old_locus_tag="J6O21_25050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228923.1"
                     /GO_function="GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA]"
                     /GO_process="GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /name="cob(I)yrinic acid a,c-diamide adenosyltransferase"
     gene            complement(4541..4894)
                     /locus_tag="J6O21_RS25205"
                     /old_locus_tag="J6O21_25055"
     CDS             complement(4541..4894)
                     /locus_tag="J6O21_RS25205"
                     /old_locus_tag="J6O21_25055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol dehydratase reactivase beta/small
                     subunit family protein"
                     /protein_id="WP_002917678.1"
                     /translation="MSLSPPGVRLFYDPRGHHAGAINELCWGLEEQGVPCQTITYDGGG
                     DAAALGALAARSSPLRVGIGLSASGEIALTHAQLPADAPLATGHVTDSDDHLRTLGANA
                     GQLVKVLPLSERN"
                     /name="glycerol dehydratase reactivase beta/small subunit
                     family protein"
     gene            complement(4915..5148)
                     /locus_tag="J6O21_RS25290"
     CDS             complement(4915..5148)
                     /locus_tag="J6O21_RS25290"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF012678.3"
                     /GO_function="GO:0046872 - metal ion binding [Evidence
                     IEA]; GO:0016491 - oxidoreductase activity [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="WP_263298514.1"
                     /translation="MGENITGLSTLDAAEKAIAAIRRLSMDIGIPQHLRDLGVKEADFP
                     YMAEMALKDGNAFSNPRKGNEQEIAAIFRQAF"
                     /name="iron-containing alcohol dehydrogenase"
     gene            complement(5148..5525)
                     /locus_tag="J6O21_RS25295"
                     /pseudo=""
     CDS             complement(5148..5525)
                     /locus_tag="J6O21_RS25295"
                     /inference="COORDINATES: protein motif:HMM:NF012678.3"
                     /GO_function="GO:0046872 - metal ion binding [Evidence
                     IEA]; GO:0016491 - oxidoreductase activity [Evidence IEA]"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /name="iron-containing alcohol dehydrogenase"
     gene            complement(5525..6076)
                     /locus_tag="J6O21_RS25300"
     CDS             complement(5525..6076)
                     /locus_tag="J6O21_RS25300"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF025065.3"
                     /GO_function="GO:0046872 - metal ion binding [Evidence
                     IEA]; GO:0016491 - oxidoreductase activity [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="WP_263298515.1"
                     /translation="MSYRMFDYLVPNVNFFGPNAISVVGERCQLLGGKKALLVTDKGLR
                     AIKDGAVDKTLHYLREAGIEVAIFDGVEPNPKDTNVRDGLAVFRREQCDIIVTVGGGSP
                     HDCGKGIGIAATHEGDLYQYAGIETLTNPLPPIVAVNTTAGTASEVTRHCVSTNTETKV
                     KFVIVGWRNLSRRSLSTIRF"
                     /name="iron-containing alcohol dehydrogenase"
     gene            complement(6093..>6287)
                     /locus_tag="J6O21_RS25215"
                     /old_locus_tag="J6O21_25065"
                     /pseudo=""
     CDS             complement(6093..>6287)
                     /locus_tag="J6O21_RS25215"
                     /old_locus_tag="J6O21_25065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004222489.1"
                     /GO_process="GO:0015074 - DNA integration [Evidence IEA]"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /name="IS3 family transposase"
     gene            complement(6390..7398)
                     /locus_tag="J6O21_RS25220"
                     /old_locus_tag="J6O21_25070"
                     /pseudo=""
     CDS             complement(6390..7398)
                     /locus_tag="J6O21_RS25220"
                     /old_locus_tag="J6O21_25070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000019445.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /name="IS5 family transposase"
ORIGIN
        1 taacatcggg gtgtactaat caacggggag caggtcagac agcccgcctc gccggtcacc
       61 gcctgaatcg cggtgagtac cgcatcgacg ctcggcgagg cgaacagatc gccgcagacc
      121 gcggcggtca gcatgccttt accaataaag ccgacgtgcg ccggttcatg gccggagccg
      181 ccgccggaaa tcaccgccac gttgttttta ttcagatcgc ggcgtaccac cacacggatc
      241 gccgggtcgc tctccagacg cgccaggtta ttccacgggc tggtgataat tgtaccgtcg
      301 atcacgtcgc taacgagatg ggggcgttga ttaaaaaaga attgagacat aacggctcct
      361 cgaggttgag tgtaagacaa ccccccgaat gcatgttcat ggtgcggagg cgatagagag
      421 attctggcga agtcagcatg gaaaaaaaca acacgaaaat gagtttcatt acgaaacgtg
      481 attgctgttt tttgttccga aatgaaactt aattaggcga gaaatcatca caatgcgaac
      541 tcgctcgact ttttcctcct gcgatcagag caggccacac ggcgcagtca gtcttttttg
      601 cggggcttta cccaggatgg cgcgtaattg gggaaattta tcatcgtgtt tgtcggtaat
      661 ttcgcggtaa cggaacggat cgagatcgca aatctcatta tttttgtggt taagcgttcc
      721 aatatgaaac gcatgtcatt tttatctgtt ccatatcaga acaaaatgac gaaagatttt
      781 tgtacacgcc tcgccataca gttattacat cgctccggta cgtccgcttt cgccgtcaac
      841 atcaggctaa acgggttaac gccggggctc accatgtaaa aacatgtact tatttgagga
      901 tgaaaggaat gctaaaagtt attcaatctc cagccaaata tctccagggt cctgacgctt
      961 ctaccttatt tggtcaatac gctaaaaatc tggcggacag ctttttcgtg attgcggacg
     1021 acttcgtcat gaagctggcg ggagaaaagg tactcaatgg cctgcacagc catgacatta
     1081 gctgccacgc ggaacgcttc aaccggtgaa tgcagccatg ttgaaattaa tcgcctgatt
     1141 gccattctga aaaaacacgg ttgccgcggc gtggtcggga tcggcggtgg taaaaccctc
     1201 gataccgcga aggcgatcgg ttactaccag aagctgccgg tggtggtgat cccgaccatc
     1261 gcctcgaccg atgcgccgac cagcgcgctg tcggtgatct acaccgaagc gggcgagttt
     1321 gaagagtatc tgatctatcc gaaaaacccg gatatggtgg tgatggacac ggcgattatc
     1381 gccaaagcgc cggtacgcct gctggtctcc ggcatgggcg atgcgctctc cacctggttc
     1441 gaggccaaag cttgctacga tgcgcgcgcc accagcatgg ccggaggaca gtccaccgag
     1501 gcggcgctga gcctcgcccg cctgtgctat gatacgctgc tggcggaggg cgaaaaggcc
     1561 cgtctggcgg cgcaggcggg gtagtgaccg aagcgctgga gcgcatcatc gaggcgaaca
     1621 cttacctcag cggcattggc tttgaaagcg gtggcctggc cgccgcccat gcaatccaca
     1681 acggtttcac cattcttgaa gagtgccatc acctgtatca cggtgagaaa gtggccttcg
     1741 gtaccctggc gcagctggtg ctacagaaca gcccgatgga cgagattgaa acggtgctgg
     1801 gcttctgcca gcgcgtcggc ctgccggtga cgctcgcgca gatgggcgtc aaagagggga
     1861 tcgacgagaa aatcgccgcg gtggcgaaag ccacctgcgc ggaagggaaa ccatccacaa
     1921 tatgccgttt gcggtgaccc cggagagcgt ccatgccgct atcctcaccg ccgatctatt
     1981 aggccagcag tggctggcgc gttaattcgc ggtggctaaa ccgctggccc aggtcagcgg
     2041 tttttctttc tcccctccgg cagtcgctgc cggaggggtt ctctatggta caacgcggaa
     2101 aaggatatga ctgttcagac tcaggatacc gggaaggcgg tctcttccgt catcgcccag
     2161 tcatggcacc gctgcagcaa gtttatgcag cgcgaaacct ggcaaacgcc gcaccaggcc
     2221 cagggcctga ccttcgactc catctgtcgg cgtaaaaccg cgctgctgac catcggtcag
     2281 gcggcgctgg aagacgcctg ggagtttatg gacggtcgcc cctgcgcgct gtttattctt
     2341 gatgagtctg cctgcatcct cagccgttgc ggcgagccgc aaaccctggc ccagctggct
     2401 gccctgggat ttcgcgacgg cagctattgt gctgagggca ttatcggcac ctgcgcgctg
     2461 tcgctggccg cgatgcaggg ccagccgatc aacaccgccg gcgatcggca ttttaagcag
     2521 gcgctacagc catggagttt ttgctcgacg ccggtgtttg ataaccacgg gcggctgttc
     2581 ggctctattt cgctttgctg tctggtcgag caccagtcca gcgccgacct ctccctgacg
     2641 ctggccatcg cccgcgaggt gggtaactcc ctgctcaccg acagcctgct ggcggagtcc
     2701 aaccgtcacc ttaatcagat gtacggcctg ctggagagca tggacgatgg ggtgatggcg
     2761 tggaacgaac agggcgtgct gcagtttctc aatgttcagg cggcgagact gctgcatctt
     2821 gatgttcagg ccagccaggg gaaaaatatc gccgatctgg tggccctccg gcgctgctgc
     2881 gcgcgccatc aaacacgccc tgcggcctga atcacgtcga agtcaccttt gaaagtcagc
     2941 atcagtttgt cgatgcggtg atcaccttaa aaccgattgt cgaggcgcaa ggcaacagtt
     3001 ttattctgct gctgcaccgg tggagcagat gcggcagctg atgaccagcc agctcggtaa
     3061 agtgagccac acctttgagc agatgtctgc cgacgatccg gaaacccgac gccctgatcc
     3121 actttggccg ccaggcggcg cagtggcttc cggtgctgtg cggcgaagag ggggttgggg
     3181 aaagggctgc tgagcaaggg tatccacaat gacggcttga acgggcgggc ggcccctaca
     3241 tcgccgtcaa ctgccagcta tatgccgaca ggatgctggg ccaggacttt atgggcagcg
     3301 ccctaccgac gatgaaaaat ggtcccctga gccgccttga gctggccaac ggcggcaccc
     3361 tgtttctgga aaagatcgag tatctggcgc cggagctgca gtcggccctg ctgcaggtga
     3421 ttaagcaggg cgtgctcacc cgcctcgacg cccggccctg atcccggtgg atgtgaaggt
     3481 gattgccacc accaccgtcg atctggccaa tctggtggaa cagaaccgct ttagccgcca
     3541 gctgtactat gcgctgcact cctttgagat cgtcatcccg ccgctgcgcg cccgacgcaa
     3601 cgatagtatt ccgtcgctga agaataaccg gtttgaagag cctggggaag cgtttctctt
     3661 cgcgactgaa agtggacgat gacgcgctgg cgcagctggt ggcctactca tggccgggga
     3721 atgattttga gctcaacagc gtcattgaga atatcgccat cagcagcgac aacggccaca
     3781 ttcgcctgag taatctgccg gaatatctct tttccgagcg gccgggcggg gatagcgcgt
     3841 catcgctgct gccggccagc ctgaccttta gcgccatcga aaaaggaagc tattattcac
     3901 gccgcccggg tgaccagcgg gcgggtgcag gagatgtcgc agctgctcaa tatcggccgc
     3961 accaccctgt ggcgcaaaat gaagcagtac gatattgacg ccagccattc aagcgcaagc
     4021 atcaggccta gtcctcctcg atacgcgcca tggagaacag ggcatccgac aggcgattgc
     4081 tgcagcgttt gagcgcgtcg cgcagcggat gcgcgcgatc catggccgtc agcaggcgtt
     4141 cgagccgacg ggactgggtg cgcgccacgt gcagctgggc agaggcgaga ttcctccccg
     4201 ggatcacgaa ctgttttaac gggccgctct cggccatatt gcggtcgata agccgctcca
     4261 gggcggtgat ctcctcttcg ccgaccgtct ggctcaggcg ggtcaggccc cgcgcatcgc
     4321 tggccagttc agcccccagc acgaacagcg tctgctgaat atggtgcagg ctttcccgga
     4381 gcccggcgtc gcgggtcgtg gcgtagcaga cgcccagttg ggatatcagt tcatcgacgg
     4441 tgccgtaggc ctcgacgcga atatggtctt tctcgatgcg gctgccgccg tacagggcgg
     4501 tggtgccttt atccccggtg cgggtataga tacgatacat tcagtttctc tcacttaacg
     4561 gcaggacttt aaccagctgc ccggcgttgg cgccgagcgt acgcagatga tcgtcgctat
     4621 cggtgacgtg tccggtagcc agcggcgcgt ccgccggcag ctgggcatga gtgagggcta
     4681 tctcgccgga cgcgctgagc ccgataccca cccgcagggg cgagcttctg gccgccaggg
     4741 cgcccagcgc agcggcgtca ccgcctccgt cataggttat ggtctggcag gggaccccct
     4801 gctcctccag cccccagcac agctcattga tggcgccggc atggtgcccg cgcggatcgt
     4861 aaaacaggcg tacgcctggc ggtgaaagcg acatgacggt cccctcgtta acactcagaa
     4921 tgcctggcgg aaaatcgcgg caatctcctg ctcgttgcct ttacgcgggt tcgagaacgc
     4981 attgccgtct ttcagagcca tctccgccat gtaggggaag tcggcctctt ttactcccag
     5041 atcgcgcaga tgctgcggaa taccgatatc catcgacaga cgcctgatag cggcgatggc
     5101 tttttccgcc gcgtcgagag tggacagtcc ggtgatattt tcgcccatca gttcagcgat
     5161 atcggcgaat ttctcgggtt ggcgatcagg ttgtagtggg ccacatgcgg cagcaggaca
     5221 gcgttggcca cgccgtgcgg catgtcgtac aggccgccca gctggtgcgc catggcgtgc
     5281 acgtagccga ggttggcgtt attgaagcca tcccggccag cagagaagca taggccatgt
     5341 tttcccgcgc ctgcagattg ctgccgaggg ccacggcctg gcgcaggttg cgggcgatga
     5401 ggcggatcgc ctgcatggcg gcggcgtccg tcaccgggtt agcgtctttg gagatatagg
     5461 cctacggcgt gggtcagggc atccatccca gtcgccgcgg tcagggcggc cggtttaccg
     5521 atcatcagaa gcggatcgtt gatagagacc gacggcttag gttgcgccag ccgacgatca
     5581 caaacttcac tttggtttcg gtgttggtcg agacgcagtg gcgggtgacc tcgctggcgg
     5641 tgccggcggt ggtattgacc gcgacgatag gtggcagcgg gttggtcagg gtctcgattc
     5701 cggcatactg gtacagatcg ccctcatggg tggcggcgat gccgatgcct ttgccgcaat
     5761 cgtgcgggct gccgccgccc acggtgacga tgatgtcgca ctgttcgcgg cgaaacacgg
     5821 cgaggccgtc gcgcacgttg gtgtctttcg ggttcggctc gacgccgtca aagatcgcca
     5881 cctcgatccc ggcctcccgc agataatgca gggttttgtc caccgctcca tctttaattg
     5941 cccgcaggcc tttgtcggtg accagcaggg cttttttccc ccccagcagc tggcagcgtt
     6001 cgccgactac ggaaatggcg ttggggccaa aaaagttaac gtttggcacc agataatcaa
     6061 acatacgata gctcataata taccttctcg cttcaggttg tatgggtcca ccacatattg
     6121 caatttttac tctcacgtaa aatatagtcc actggcgtca tcagttcgag tgatttatgt
     6181 ggccttttgc tgttatacag caccagatat tcagccattc tttgattaaa caaattcagg
     6241 tcctcaaaaa gcagcaattc attgaattca ataaattgtt ctctaaggaa ggtgcgaata
     6301 agcaggtcat ttcttccaag ttccgctgat taaaatttcg cggatctggg ccgatttttt
     6361 tcccgcaaac accgaatcat ctatttaggc tattttttcc accatttcgg cgttatcgtt
     6421 ttactgagat cttcccactg acgtatcggg ggtccaccgg aaacaggttg gccagggtga
     6481 ataacatcgc cagttggtta tcgtttttca gcagcccctt gtatctggct ttcacgaagc
     6541 cgaactgccg cttgatgatg cgaaaacggg tgctccacct ggcacccgtt attatctcat
     6601 gtattcgatg ttgaaaaggc tgcttgtctt tcttgcgcgg atgcgctgct tcaaggtttt
     6661 taccctgccg ggacgctcgg cgatcagcca gtccatccac ctcggctggc cctcgcgctg
     6721 tggcgctcct tgcaggccgg cagcatcggc tgagacaaat tgctcctctc catgaagcag
     6781 attacccagc tgattgaggt catgctcgtt ggtcgccgtg tgcagatctg tgctgtgggt
     6841 caggccactc ttggcatcga cgacgcacgg gccttcatgc ctgtgcgccg attgcctttc
     6901 ttggtctgat gcatatcaag atcaaaagct gctctttgtt ctggtagagc tgggtgcctc
     6961 aatgatggtg gcatccacca aagtgccttt ggtcatcatg acgcctgctt cggccagcca
     7021 gcgattgatg gttctgaaca attgacgggc cagttgatgc tgctcgagca ggtggcggaa
     7081 attcagcgat ggtggtgcga tccggcaggg cgctatccag ggatattccg ggcaaacagg
     7141 cgcatgaggc gatttcgtac agagcataag gcacggcacc gctcaggttg ctttcaatgc
     7201 tgcacagtga atacgcagca tggtctccag cggatagggc cgtcggccat tgcccgcctt
     7261 gggataaaac ggctcgatga cagcggtcat attctgccat ggcagaatct gctccatgcg
     7321 ggagaggaaa atctcttttc gggtctgacg gcgcttagtg ctgaattcac tatcggcgaa
     7381 ggtgagttga tggctcatga tgtccctctg ggatgcgctc cggatgaata tgatgatctc
     7441 atatcaggaa cttgttcgca ccttccc
//
