LOCUS       NZ_CP081705             2760 bp    DNA     circular CON 02-NOV-2023
DEFINITION  Escherichia coli strain AH02 plasmid unnamed4, complete sequence.
ACCESSION   NZ_CP081705
VERSION     NZ_CP081705.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN18446035
            Assembly: GCF_019969645.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2760)
  AUTHORS   Yang,X., Tran,F. and Zhang,P.
  TITLE     Genetic characterization of Escherichia coli recovered from beef
            production environments, which harbor the locus of heat resistance
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2760)
  AUTHORS   Yang,X., Tran,F. and Zhang,P.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2021) Food Safety, Agriculture & Agri-Food Canada,
            6000 C & E Trail, Lacombe, Alberta T4L 1W1, Canada
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 26-JAN-2021
            Assembly Method                   :: flye v. 2.8.2
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 68x
            Sequencing Technology             :: Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/02/2023 04:23:14
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,010
            Genes (coding)                    :: 4,712
            CDSs (with protein)               :: 4,712
            Genes (RNA)                       :: 125
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 90
            ncRNAs                            :: 13
            Pseudo Genes (total)              :: 298
            CDSs (without protein)            :: 298
            Pseudo Genes (ambiguous residues) :: 0 of 298
            Pseudo Genes (frameshifted)       :: 72 of 298
            Pseudo Genes (incomplete)         :: 222 of 298
            Pseudo Genes (internal stop)      :: 41 of 298
            Pseudo Genes (multiple problems)  :: 35 of 298
            Pseudo Genes (short protein)      :: 3 of 298
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP081705.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2760
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="AH02"
                     /db_xref="taxon:562"
                     /plasmid="unnamed4"
                     /geo_loc_name="Canada: Alberta"
                     /collection_date="2013"
     gene            join(2671..2760,1..954)
                     /gene="mobV"
                     /locus_tag="J6O02_RS25505"
                     /old_locus_tag="J6O02_25360"
     CDS             join(2671..2760,1..954)
                     /gene="mobV"
                     /locus_tag="J6O02_RS25505"
                     /old_locus_tag="J6O02_25360"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MobV family relaxase"
                     /protein_id="WP_223598072.1"
                     /translation="MRSPDWLDGRQNEGVSMANYAIMRCKKLTGIMRCKKLTGMGSVAS
                     ALQHCYRERETPNADAERTPENYCSVSQSADEAMGKLRELLPEKRRKDAVLAVEYVMTA
                     SPEWWKEATPRQQAEFFARSEQWLEKKYGKDRVVAAVVHRDEATPHLSAFVVPLTQDGR
                     LSAKEFIGGRSKMRDDQSTYAESVKKLGLERGIEGSRATHQTVQHYYESINRGTRSQVS
                     ISPEALEPRVLRKGIFTKDVEDQAAIAKRLSHAVNDGFAGTIAMASQSAQNAKRARELQ
                     KTMDSQQKRLQSVTEPFKGLSREQMTEILMMAQRFKQQNQEKEKQQRVEREKQRQMRSR
                     GMGGMER"
                     /name="mobV"
     gene            complement(1018..1344)
                     /locus_tag="J6O02_RS25510"
                     /old_locus_tag="J6O02_25365"
     CDS             complement(1018..1344)
                     /locus_tag="J6O02_RS25510"
                     /old_locus_tag="J6O02_25365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001070513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_223571429.1"
                     /translation="MNQAALITIANELNHDQMRVLMALLAELDYENYIQVAQMDIAEAL
                     TMQKTNVSRAVKNLIDFGIILEGPKIGRSKTYRLNPQFGWKGTVSNHKKALKNGLSVIQ
                     GGRT"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(1864..2469)
                     /locus_tag="J6O02_RS25515"
                     /old_locus_tag="J6O02_25370"
     CDS             complement(1864..2469)
                     /locus_tag="J6O02_RS25515"
                     /old_locus_tag="J6O02_25370"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_054623596.1"
                     /translation="MSFIKSLNKKNQTDQIEVTINVRLTQEEDHLLTLATQQSGYKRNE
                     LFYLLAKEYIFDDESLKEMAEESGAEFSEGLYQNCYVVNTNRVHHEEDDKFMISEGVAA
                     TFEDGYKEKMEQVKKGDLVFLYRSGEGIVAHGIASGDVLKKDHNGVPDKTYYQKLSGFV
                     RYEKPLTASEIQRILGRNVVFMHTLFSIRDGYKLLKKD"
                     /name="hypothetical protein"
     Typing          2..951
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="97.476"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 ataatgcgct gcaaaaagct gacgggaatg ggcagtgtgg ccagcgcttt gcagcactgc
       61 tacagggagc gtgagacgcc gaatgctgac gctgaacgga caccagaaaa ctactgctct
      121 gtgtctcagt cggcagatga ggcgatggga aagctgcggg agctgttgcc ggaaaaacgc
      181 cgtaaagatg cggtactggc tgttgaatac gttatgacgg caagccctga gtggtggaaa
      241 gaagcgacac cgcggcagca ggcggagttt ttcgcccgct ctgagcaatg gctggagaag
      301 aaatacggga aggatcgtgt tgtggcggcg gtggttcacc gggacgaggc caccccgcac
      361 ttgtcagcgt tcgtggtgcc attgacgcag gatggacggc tgtccgcgaa agagtttatt
      421 ggcgggcggt caaagatgcg tgacgatcag agcacctacg ctgaatcagt aaaaaaactg
      481 ggtcttgagc gcggcatcga gggaagccgc gccacgcatc agacagttca gcactactac
      541 gaatcgataa accgtggcac ccgtagccag gtatcgatct cgccggaggc gctggagccg
      601 cgtgtattgc ggaagggcat tttcaccaaa gacgtggaag atcaggcagc catagccaaa
      661 cgtctaagcc acgcagtgaa cgatggcttt gcaggaacca tcgctatggc ctctcagagc
      721 gcccagaatg caaaaagagc gcgcgagcta cagaagacta tggattcgca gcagaaacgc
      781 ctacagagcg ttacagagcc ttttaagggg ctttccagag agcagatgac agaaatcctg
      841 atgatggctc agcggtttaa gcagcaaaac caggagaagg agaaacagca gcgtgttgaa
      901 cgtgagaaac agcgacagat gcgatcgcgt ggcatgggcg gtatggagcg ttagttttga
      961 tgcgatgcag gatagggcta acgccgcaaa atgacgccat ttgcggcatt ccagagatca
     1021 ggttctgccg ccctgtataa cgctgaggcc gtttttcagt gcttttttgt ggttgctgac
     1081 agtacctttc cagccgaact ggggatttag gcggtacgtt ttgctccgcc caatcttcgg
     1141 cccttccagt atgatcccga aatcaatcag gttttttacc gcacgactga catttgtttt
     1201 ttgcatagtc agcgcttccg caatatccat ctgcgcaact tggatgtagt tctcgtaatc
     1261 cagttctgcg agcaaggcca tcagcacacg catctggtca tggtttagct cattggcaat
     1321 ggtaattagc gcggcttggt tcatagtaaa gtgcctctgg aatgatgact tttgtttagg
     1381 acgaatcact gctacaaaac cacctaattc ctcccctgta gtttggtcta cctgcgtgat
     1441 tctccgcatc taaaaatctc cattcaggaa aaaatagtta tctctcaaga taactaagtt
     1501 atctctagag ataactattg tcaaaaactt tccgcttgtg gcgcggcttc ccagccatcc
     1561 ccttctgtat cttattactc tccacaatca cacatcggca aaatcaggcg tttttctgcc
     1621 cgttcggtgc gaacgaattt ctatgtacgg gaagtggaaa ttccgcttcc tcgctcattc
     1681 gctcactacg ttcggtcatg agtctgcggc gagcggttct tgatttttta aaacgatatt
     1741 cggagcttta tttcgtcaag ggcgagcgta gcgagttcat ttacccttga cgatgcactg
     1801 taaaaaacac ttccggaagg tggttggtgt cacaaagtga tgccaaccac ctgtagactg
     1861 ggtttaatct tttttaagca gtttatagcc atctctaatg ctaaatagcg tgtgcataaa
     1921 cacaacgttt ctccccagta ttctctggat ctcagacgcg gttagtggtt tttcgtacct
     1981 aacaaagcct gatagcttct ggtagtaggt cttgtctggc acgccattat gatctttctt
     2041 taaaacatca ccactggcga taccatgtgc aacaattcct tcgccggagc gataaagaaa
     2101 gactaaatct ccttttttaa cctgctccat tttttcctta taaccatctt caaatgttgc
     2161 cgctacgccc tctgaaatca tgaacttatc atcttcttca tgatgaactc tgttagtgtt
     2221 aaccacataa caattttggt ataagccttc tgaaaactca gcgccagact cctcagccat
     2281 ttcctttaag ctttcatcat caaagatgta ttcctttgct aggaggtaga atagctcgtt
     2341 ccttttataa ccagattgct gggttgctaa tgtcagtaaa tggtcctctt cttgggttaa
     2401 cctaacgtta atcgtcactt ctatttggtc tgtttgattt tttttgttta gcgattttat
     2461 gaatgacatc ttactttcct caccagagtt aatgtactaa tcaatgatag tgaatcacta
     2521 cattgaaaca gaagcattga tcacatcttt gatttaagca atgactttat gtcatatatt
     2581 tttattaagt tgaatttaac gaacgatttt gcgtagcaaa gtctagtgag tacactatca
     2641 tcctgatagt ggcccgccgg aggcatcgca atgcgatccc ccgactggct ggatggacgc
     2701 cagaacgagg gggtaagcat ggcgaattac gcgataatgc gctgcaaaaa gctgacggga
//
