LOCUS       NZ_CP081671             2728 bp    DNA     circular CON 21-JUN-2023
DEFINITION  Escherichia coli strain 14-AB00641 plasmid pQNR14-AB00641, complete
            sequence.
ACCESSION   NZ_CP081671
VERSION     NZ_CP081671.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN20835128
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2728)
  AUTHORS   Juraschek,K.A. and Hammerl,J.A.
  TITLE     qnrB-carrying ESBL
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2728)
  AUTHORS   Juraschek,K.A. and Hammerl,J.A.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-AUG-2021) Biological Safety, German Federal Institute
            for Risk Assessment, Diedersdorfer Weg 1, Berlin, Berlin 12277,
            Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SPAdes v. v3.14
            Genome Representation             :: Partial
            Expected Final Version            :: Yes
            Genome Coverage                   :: 30x
            Sequencing Technology             :: Illumina NextSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 06/21/2023 14:25:24
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3
            CDSs (total)                      :: 3
            Genes (coding)                    :: 2
            CDSs (with protein)               :: 2
            Genes (RNA)                       :: 0
            tRNAs                             :: 0
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP081671.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2728
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="14-AB00641"
                     /isolation_source="Poultry, meat"
                     /db_xref="taxon:562"
                     /plasmid="pQNR14-AB00641"
                     /geo_loc_name="Germany"
                     /collection_date="2014"
     gene            complement(395..1039)
                     /locus_tag="K5596_RS00005"
                     /old_locus_tag="K5596_00005"
     CDS             complement(395..1039)
                     /locus_tag="K5596_RS00005"
                     /old_locus_tag="K5596_00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017900990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolone resistance pentapeptide repeat protein
                     QnrB19"
                     /protein_id="WP_012954666.1"
                     /translation="MTLALVGEKIDRNRFTGEKVENSTFFNCDFSGADLSGTEFIGCQF
                     YDRESQKGCNFSRAMLKDAIFKSCDLSMADFRNVSALGIEIRHCRAQGADFRGASFMNM
                     ITTRTWFCSAYITNTNLSYANFSKVVLEKCELWENRWMGTQVLGATFSGSDLSGGEFST
                     FDWRAANFTHCDLTNSELGDLDIRGVDLQGVKLDSYQASLLMERLGIAVIG"
                     /name="quinolone resistance pentapeptide repeat protein
                     QnrB19"
     gene            complement(1133..>1288)
                     /locus_tag="K5596_RS00010"
                     /old_locus_tag="K5596_00010"
                     /pseudo=""
     CDS             complement(1133..>1288)
                     /locus_tag="K5596_RS00010"
                     /old_locus_tag="K5596_00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309907.2"
                     /GO_function="GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /name="helix-turn-helix domain-containing protein"
     gene            2356..2592
                     /locus_tag="K5596_RS00015"
                     /old_locus_tag="K5596_00015"
     CDS             2356..2592
                     /locus_tag="K5596_RS00015"
                     /old_locus_tag="K5596_00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001676031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_012772745.1"
                     /translation="MARLTNLTPAEKKFLDDAVAAAERASGKKLNQPNRHIVLNRARAQ
                     IELQRYADRQRALREDERQQSDFAWSRPRAPRR"
                     /name="hypothetical protein"
     ARG             complement(399..1039)
                     /name="qnrB19"
                     /gene="qnrB19"
                     /gene_name="quinolone resistance pentapeptide repeat
                     protein QnrB19"
                     /tool="amrfinderplus"
                     /drug_class="QUINOLONE"
                     /identity="100.0"
                     /coverage="100.0"
     Typing          2018..2191
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="2.5600000000000002e-86"
     Typing          complement(1548..2039)
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="92.51"
                     /coverage="81.0"
                     /evalue="0.0"
ORIGIN
        1 gctgacgctg cgaaatcacc tgccattcac ctgcgtgcat agccatgcag tcgcgcagaa
       61 atcatcaggg gcgtttttcc gggatactgg tgaggaaagg tgctggaaac ggccctgatg
      121 cagcaggatt gcgctgaggg cgcctgcggg ggtgcgggca agggatgtta tgtttaatcg
      181 gtctgaccgt caggtacaaa aaaaagccct tagggggctt tttttttgct atgagttagc
      241 catattttcg tgcgaatttt tcatcggaat cgcatagata aataataaac tcaataaacg
      301 cgatgatcgc gggtatgaat gtccagcaaa aaattagata caggaacccc ttaaaagtaa
      361 atcgtcaaac gcatctcccg gtgtaaacgc tgaactaacc aatcacagcg atgccaagcc
      421 gctccatgag caacgatgcc tggtagctgt ctaacttaac gccttgtaaa tcaacacccc
      481 gaatatctaa gtcacctaac tccgaattgg tcagatcgca atgtgtgaag tttgctgctc
      541 gccagtcgaa agtcgaaaac tcgccgccgg agagatctga accactgaac gtcgcaccca
      601 gtacctgagt ccccatccag cggttttccc acagctcaca cttttccaac acgactttcg
      661 aaaaattggc gtagcttaga ttagtattag tgatatatgc gctgcaaaac caggtgcgcg
      721 tggtgatcat attcataaag cttgcaccgc ggaaatctgc gccttgtgcg cggcagtggc
      781 gaatttcaat gcccaaggca ctgacgttgc ggaaatctgc cattgataaa tcacagcttt
      841 tgaaaatggc atctttcagc attgcgcgac taaaattgca ccctttctga ctttcgcgat
      901 catagaactg gcagccgata aattcagtgc cgctcaggtc ggcacctgaa aaatcgcagt
      961 taaaaaatgt actattttca actttctcac cggtgaagcg atttctgtca attttttcgc
     1021 caactaatgc cagagtcata tctatgcctg tgtttttata cagtaatgac gccatggtaa
     1081 accttatgac gttatgcgtc aaacccgcca gtatgacacc tgcaaatgcg tattagatct
     1141 ggtgtttctt cagcaaggca cgtagctgat gataagtcag acccagtaat tcagcggctt
     1201 ttttctggtt aaatttagcc tgctgtaagc tggtttgtag aaagtctttc tcttgctgct
     1261 gctggaattc acgcagatcc agcgttataa cgatgtgtat cctatgtgct cggattggat
     1321 agatttccat tacggtgaag gatcttcttg agatcctttc tgtggcgtcg taatctcttg
     1381 ctctgtaaac gaaaaaaccg ccctgcaagg cggtttttcg aaggttctca gagcagcaac
     1441 tctttgaacc gtggtaactg gcttacgagg agcgcagtaa ccaaatctgt tctttcagtt
     1501 tagccttaac aggcgcataa cttcaagact aactcctcta aatcagttac cagtggctgc
     1561 tgccagtggc gataagtcgt gtctttccgg gttggactca agatgatagt taccggataa
     1621 ggcgcagcag tcggactgaa cggggggttc gtgcatacag tccatccagg agcgaactgc
     1681 cttcgcggaa ctgagtgtca ggcgtggaat gagataaacg cggccataac agcggaatga
     1741 caccggtaaa ccgaaaggca ggaatgcggg ggagcacgag ggagccacca gggggaaacg
     1801 cctggtatct ttaagccgca tcgggtttcg ccaccactga tttgagcgtc agatttcatg
     1861 atgtttgtca ggggggcgga gcctatggaa aaacggcttc gccgcggcct tatctcttcc
     1921 ctgttaagct ccttcctggt atctcccagg aaatttccgc cccgcctgta agccggcacc
     1981 gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaatatatc
     2041 ctgtactaca tatcctgctg acgcgctggt gccgcctttt ttctcctgct acatgaagca
     2101 cttcactgat ttccacatcc gtgccaacat agtcagccag tatacactcc gctagcgcta
     2161 cgtgactggt tcagggctgc gccccgaaac cggctaaaag ctaatgacgc gcctgcggct
     2221 tgtccaacgc ctcacggcac cggattaaaa tccgctcccg ttcaccgttt ctgactgcct
     2281 ttcaccgtcg ctggcgcttc atcgtttcta ctgtgtctgg cagactgtgc tgatttattc
     2341 tgactggaga acgttatggc cagactgaca aacttgacgc ctgcagagaa aaaatttctt
     2401 gatgatgctg ttgccgcagc tgaaagagcg tcaggtaaaa agctaaatca gcctaaccgg
     2461 cacatcgtcc tgaacagagc gcgtgcgcaa attgagttgc agcgttacgc cgaccgccag
     2521 cgtgcattac gtgaggatga gcggcagcag tccgattttg cctggtcacg tccgcgcgcg
     2581 ccgcgccgct gattttggct tttggcggtt tagtggtgct gacgctgcga aatcacctgc
     2641 cattcacctg cgtgcatagc catgcagtcg cgcagaaatc atcaggggcg tttttccggg
     2701 atactggtga ggaaaggtgc tggaaacg
//
