LOCUS       NZ_CP081670             2826 bp    DNA     circular CON 20-OCT-2023
DEFINITION  Escherichia coli strain 14-AB01030 plasmid pQNR14-AB01030, complete
            sequence.
ACCESSION   NZ_CP081670
VERSION     NZ_CP081670.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN20835129
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2826)
  AUTHORS   Juraschek,K.A. and Hammerl,J.A.
  TITLE     qnrB-carrying ESBL
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2826)
  AUTHORS   Juraschek,K.A. and Hammerl,J.A.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-AUG-2021) Biological Safety, German Federal Institute
            for Risk Assessment, Diedersdorfer Weg 1, Berlin, Berlin 12277,
            Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SPAdes v. v3.14
            Genome Representation             :: Partial
            Expected Final Version            :: Yes
            Genome Coverage                   :: 30x
            Sequencing Technology             :: Illumina NextSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 10/20/2023 04:46:55
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3
            CDSs (total)                      :: 3
            Genes (coding)                    :: 2
            CDSs (with protein)               :: 2
            Genes (RNA)                       :: 0
            tRNAs                             :: 0
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP081670.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2826
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="14-AB01030"
                     /isolation_source="Poultry, feces"
                     /db_xref="taxon:562"
                     /plasmid="pQNR14-AB01030"
                     /geo_loc_name="Germany"
                     /collection_date="2016"
     gene            495..731
                     /locus_tag="K5580_RS00005"
                     /old_locus_tag="K5580_00005"
     CDS             495..731
                     /locus_tag="K5580_RS00005"
                     /old_locus_tag="K5580_00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001676031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_012772745.1"
                     /translation="MARLTNLTPAEKKFLDDAVAAAERASGKKLNQPNRHIVLNRARAQ
                     IELQRYADRQRALREDERQQSDFAWSRPRAPRR"
                     /name="hypothetical protein"
     gene            complement(1233..1877)
                     /locus_tag="K5580_RS00010"
                     /old_locus_tag="K5580_00010"
     CDS             complement(1233..1877)
                     /locus_tag="K5580_RS00010"
                     /old_locus_tag="K5580_00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017900990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolone resistance pentapeptide repeat protein
                     QnrB19"
                     /protein_id="WP_012954666.1"
                     /translation="MTLALVGEKIDRNRFTGEKVENSTFFNCDFSGADLSGTEFIGCQF
                     YDRESQKGCNFSRAMLKDAIFKSCDLSMADFRNVSALGIEIRHCRAQGADFRGASFMNM
                     ITTRTWFCSAYITNTNLSYANFSKVVLEKCELWENRWMGTQVLGATFSGSDLSGGEFST
                     FDWRAANFTHCDLTNSELGDLDIRGVDLQGVKLDSYQASLLMERLGIAVIG"
                     /name="quinolone resistance pentapeptide repeat protein
                     QnrB19"
     gene            complement(1971..>2126)
                     /locus_tag="K5580_RS00015"
                     /old_locus_tag="K5580_00015"
                     /pseudo=""
     CDS             complement(1971..>2126)
                     /locus_tag="K5580_RS00015"
                     /old_locus_tag="K5580_00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309907.2"
                     /GO_function="GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /name="helix-turn-helix domain-containing protein"
     ARG             complement(1237..1877)
                     /name="qnrB19"
                     /gene="qnrB19"
                     /gene_name="quinolone resistance pentapeptide repeat
                     protein QnrB19"
                     /tool="amrfinderplus"
                     /drug_class="QUINOLONE"
                     /identity="100.0"
                     /coverage="100.0"
     Typing          157..330
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="2.5600000000000002e-86"
     Typing          complement(2..184)
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="92.391"
                     /coverage="95.0"
                     /evalue="1.93e-67"
     Typing          complement(2386..2826)
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="91.629"
                     /coverage="81.0"
                     /evalue="1.9199999999999998e-172"
ORIGIN
        1 tgtttgtcag gggggcggag cctatggaaa aacggcttcg ccgcggcctt atctcttccc
       61 tgttaagctc cttcctggta tctcccagga aatttccgcc ccgcctgtaa gccggcaccg
      121 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaatatatcc
      181 tgtactacat atcctgctga cgcgctggtg ccgccttttt tctcctgcta catgaagcac
      241 ttcactgatt tccacatccg tgccaacata gtcagccagt atacactccg ctagcgctac
      301 gtgactggtt cagggctgcg ccccgaaacc ggctaaaagc taatgacgcg cctgcggctt
      361 gtccaacgcc tcacggcacc ggattaaaat ccgctcccgt tcaccgtttc tgactgcctt
      421 tcaccgtcgc tggcgcttca tcgtttctac tgtgtctggc agactgtgct gatttattct
      481 gactggagaa cgttatggcc agactgacaa acttgacgcc tgcagagaaa aaatttcttg
      541 atgatgctgt tgccgcagct gaaagagcgt caggtaaaaa gctaaatcag cctaaccggc
      601 acatcgtcct gaacagagcg cgtgcgcaaa ttgagttgca gcgttacgcc gaccgccagc
      661 gtgcattacg tgaggatgag cggcagcagt ccgattttgc ctggtcacgt ccgcgcgcgc
      721 cgcgccgctg attttggctt ttggcggttt agtggtgctg acgctgcgaa atcacctgcc
      781 attcacctgc gtgcatagcc atgcagtcgc gcagaaatca tcaggggcgt ttttccggga
      841 tactggtgag gaaaggtgct ggaaacggcc ctgatgcagc aggattgcgc tgagggcgcc
      901 tgcgggggtg cgggcaaggg atgttatgtt taatcggtct gaccgtcagg tacaaaaaaa
      961 agcccttagg gggctttttt tttgctatga gttagccata ttttcgttaa gacatggctt
     1021 catgattcgg gagtggttat tcgttgttct cgcgaaaatg aattggttag atattcatca
     1081 aattcctgtc cagaatgcag cattagatgg catgtcgttg atgcggtagg tcaggatatt
     1141 cgtccactgg agaaaacgtt tcacaatgct tgccaggagt cgtactggtt aaaagtaaat
     1201 cgtcaaacgc atctcccggt gtaaacgctg aactaaccaa tcacagcgat gccaagccgc
     1261 tccatgagca acgatgcctg gtagctgtct aacttaacgc cttgtaaatc aacaccccga
     1321 atatctaagt cacctaactc cgaattggtc agatcgcaat gtgtgaagtt tgctgctcgc
     1381 cagtcgaaag tcgaaaactc gccgccggag agatctgaac cactgaacgt cgcacccagt
     1441 acctgagtcc ccatccagcg gttttcccac agctcacact tttccaacac gactttcgaa
     1501 aaattggcgt agcttagatt agtattagtg atatatgcgc tgcaaaacca ggtgcgcgtg
     1561 gtgatcatat tcataaagct tgcaccgcgg aaatctgcgc cttgtgcgcg gcagtggcga
     1621 atttcaatgc ccaaggcact gacgttgcgg aaatctgcca ttgataaatc acagcttttg
     1681 aaaatggcat ctttcagcat tgcgcgacta aaattgcacc ctttctgact ttcgcgatca
     1741 tagaactggc agccgataaa ttcagtgccg ctcaggtcgg cacctgaaaa atcgcagtta
     1801 aaaaatgtac tattttcaac tttctcaccg gtgaagcgat ttctgtcaat tttttcgcca
     1861 actaatgcca gagtcatatc tatgcctgtg tttttataca gtaatgacgc catggtaaac
     1921 cttatgacgt tatgcgtcaa acccgccagt atgacacctg caaatgcgta ttagatctgg
     1981 tgtttcttca gcaaggcacg tagctgatga taagtcagac ccagtaattc agcggctttt
     2041 ttctggttaa atttagcctg ctgtaagctg gtttgtagaa agtctttctc ttgctgctgc
     2101 tggaattcac gcagatccag cgttataacg atgtgtatcc tatgtgctcg gattggatag
     2161 atttccatta cggtgaagga tcttcttgag atcctttctg tggcgtcgta atctcttgct
     2221 ctgtaaacga aaaaaccgcc ctgcaaggcg gtttttcgaa ggttctcaga gcagcaactc
     2281 tttgaaccgt ggtaactggc ttacgaggag cgcagtaacc aaatctgttc tttcagttta
     2341 gccttaacag gcgcataact tcaagactaa ctcctctaaa tcagttacca gtggctgctg
     2401 ccagtggcga taagtcgtgt ctttccgggt tggactcaag atgatagtta ccggataagg
     2461 cgcagcagtc ggactgaacg gggggttcgt gcatacagtc catccaggag cgaactgcct
     2521 tcgcggaact gagtgtcagg cgtggaatga gataaacgcg gccataacag cggaatgaca
     2581 ccggtaaacc gaaaggcagg aatgcggggg agcacgaggg agccaccagg gggaaacgcc
     2641 tggtatcttt aagccgcatc gggtttcgcc accactgatt tgagcgtcag atttcatgat
     2701 gtttgtcagg ggggcggagc ctatggaaaa acggcttcgc cgcggcctta tctcttccct
     2761 gttaagctcc ttcctggtat ctcccaggaa atttccgccc cgcctgtaag ccggcaccgc
     2821 tcgccg
//
