LOCUS       NZ_CP081669             2728 bp    DNA     circular CON 21-JUN-2023
DEFINITION  Escherichia coli strain 16-AB00831 plasmid pQNR16-AB00831, complete
            sequence.
ACCESSION   NZ_CP081669
VERSION     NZ_CP081669.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN20835130
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2728)
  AUTHORS   Juraschek,K.A. and Hammerl,J.A.
  TITLE     qnrB-carrying ESBL
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2728)
  AUTHORS   Juraschek,K.A. and Hammerl,J.A.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-AUG-2021) Biological Safety, German Federal Institute
            for Risk Assessment, Diedersdorfer Weg 1, Berlin, Berlin 12277,
            Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SPAdes v. v3.14
            Genome Representation             :: Partial
            Expected Final Version            :: Yes
            Genome Coverage                   :: 30x
            Sequencing Technology             :: Illumina NextSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 06/21/2023 14:51:11
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3
            CDSs (total)                      :: 3
            Genes (coding)                    :: 2
            CDSs (with protein)               :: 2
            Genes (RNA)                       :: 0
            tRNAs                             :: 0
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP081669.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2728
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="16-AB00831"
                     /isolation_source="Poultry, meat"
                     /db_xref="taxon:562"
                     /plasmid="pQNR16-AB00831"
                     /geo_loc_name="Germany"
                     /collection_date="2016"
     gene            complement(252..488)
                     /locus_tag="K5587_RS00005"
                     /old_locus_tag="K5587_00005"
     CDS             complement(252..488)
                     /locus_tag="K5587_RS00005"
                     /old_locus_tag="K5587_00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001676031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_012772745.1"
                     /translation="MARLTNLTPAEKKFLDDAVAAAERASGKKLNQPNRHIVLNRARAQ
                     IELQRYADRQRALREDERQQSDFAWSRPRAPRR"
                     /name="hypothetical protein"
     gene            <1556..1711
                     /locus_tag="K5587_RS00010"
                     /old_locus_tag="K5587_00010"
                     /pseudo=""
     CDS             <1556..1711
                     /locus_tag="K5587_RS00010"
                     /old_locus_tag="K5587_00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707212.3"
                     /GO_function="GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /name="helix-turn-helix domain-containing protein"
     gene            1805..2449
                     /locus_tag="K5587_RS00015"
                     /old_locus_tag="K5587_00015"
     CDS             1805..2449
                     /locus_tag="K5587_RS00015"
                     /old_locus_tag="K5587_00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017900990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolone resistance pentapeptide repeat protein
                     QnrB19"
                     /protein_id="WP_012954666.1"
                     /translation="MTLALVGEKIDRNRFTGEKVENSTFFNCDFSGADLSGTEFIGCQF
                     YDRESQKGCNFSRAMLKDAIFKSCDLSMADFRNVSALGIEIRHCRAQGADFRGASFMNM
                     ITTRTWFCSAYITNTNLSYANFSKVVLEKCELWENRWMGTQVLGATFSGSDLSGGEFST
                     FDWRAANFTHCDLTNSELGDLDIRGVDLQGVKLDSYQASLLMERLGIAVIG"
                     /name="quinolone resistance pentapeptide repeat protein
                     QnrB19"
     ARG             1806..2446
                     /name="qnrB19"
                     /gene="qnrB19"
                     /gene_name="quinolone resistance pentapeptide repeat
                     protein QnrB19"
                     /tool="amrfinderplus"
                     /drug_class="QUINOLONE"
                     /identity="100.0"
                     /coverage="100.0"
     Typing          complement(654..827)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="2.5600000000000002e-86"
     Typing          806..1297
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="92.51"
                     /coverage="81.0"
                     /evalue="0.0"
ORIGIN
        1 aaaaaaaagc cccctaaggg cttttttttg tacctgacgg tcagaccgat taaacataac
       61 atcccttgcc cgcacccccg caggcgccct cagcgcaatc ctgctgcatc agggccgttt
      121 ccagcacctt tcctcaccag tatcccggaa aaacgcccct gatgatttct gcgcgactgc
      181 atggctatgc acgcaggtga atggcaggtg atttcgcagc gtcagcacca ctaaaccgcc
      241 aaaagccaaa atcagcggcg cggcgcgcgc ggacgtgacc aggcaaaatc ggactgctgc
      301 cgctcatcct cacgtaatgc acgctggcgg tcggcgtaac gctgcaactc aatttgcgca
      361 cgcgctctgt tcaggacgat gtgccggtta ggctgattta gctttttacc tgacgctctt
      421 tcagctgcgg caacagcatc atcaagaaat tttttctctg caggcgtcaa gtttgtcagt
      481 ctggccataa cgttctccag tcagaataaa tcagcacagt ctgccagaca cagtagaaac
      541 gatgaagcgc cagcgacggt gaaaggcagt cagaaacggt gaacgggagc ggattttaat
      601 ccggtgccgt gaggcgttgg acaagccgca ggcgcgtcat tagcttttag ccggtttcgg
      661 ggcgcagccc tgaaccagtc acgtagcgct agcggagtgt atactggctg actatgttgg
      721 cacggatgtg gaaatcagtg aagtgcttca tgtagcagga gaaaaaaggc ggcaccagcg
      781 cgtcagcagg atatgtagta caggatatat tccgcttcct cgctcactga ctcgctgcgc
      841 tcggtcgttc ggctgcggcg agcggtgccg gcttacaggc ggggcggaaa tttcctggga
      901 gataccagga aggagcttaa cagggaagag ataaggccgc ggcgaagccg tttttccata
      961 ggctccgccc ccctgacaaa catcatgaaa tctgacgctc aaatcagtgg tggcgaaacc
     1021 cgatgcggct taaagatacc aggcgtttcc ccctggtggc tccctcgtgc tcccccgcat
     1081 tcctgccttt cggtttaccg gtgtcattcc gctgttatgg ccgcgtttat ctcattccac
     1141 gcctgacact cagttccgcg aaggcagttc gctcctggat ggactgtatg cacgaacccc
     1201 ccgttcagtc cgactgctgc gccttatccg gtaactatca tcttgagtcc aacccggaaa
     1261 gacacgactt atcgccactg gcagcagcca ctggtaactg atttagagga gttagtcttg
     1321 aagttatgcg cctgttaagg ctaaactgaa agaacagatt tggttactgc gctcctcgta
     1381 agccagttac cacggttcaa agagttgctg ctctgagaac cttcgaaaaa ccgccttgca
     1441 gggcggtttt ttcgtttaca gagcaagaga ttacgacgcc acagaaagga tctcaagaag
     1501 atccttcacc gtaatggaaa tctatccaat ccgagcacat aggatacaca tcgttataac
     1561 gctggatctg cgtgaattcc agcagcagca agagaaagac tttctacaaa ccagcttaca
     1621 gcaggctaaa tttaaccaga aaaaagccgc tgaattactg ggtctgactt atcatcagct
     1681 acgtgccttg ctgaagaaac accagatcta atacgcattt gcaggtgtca tactggcggg
     1741 tttgacgcat aacgtcataa ggtttaccat ggcgtcatta ctgtataaaa acacaggcat
     1801 agatatgact ctggcattag ttggcgaaaa aattgacaga aatcgcttca ccggtgagaa
     1861 agttgaaaat agtacatttt ttaactgcga tttttcaggt gccgacctga gcggcactga
     1921 atttatcggc tgccagttct atgatcgcga aagtcagaaa gggtgcaatt ttagtcgcgc
     1981 aatgctgaaa gatgccattt tcaaaagctg tgatttatca atggcagatt tccgcaacgt
     2041 cagtgccttg ggcattgaaa ttcgccactg ccgcgcacaa ggcgcagatt tccgcggtgc
     2101 aagctttatg aatatgatca ccacgcgcac ctggttttgc agcgcatata tcactaatac
     2161 taatctaagc tacgccaatt tttcgaaagt cgtgttggaa aagtgtgagc tgtgggaaaa
     2221 ccgctggatg gggactcagg tactgggtgc gacgttcagt ggttcagatc tctccggcgg
     2281 cgagttttcg actttcgact ggcgagcagc aaacttcaca cattgcgatc tgaccaattc
     2341 ggagttaggt gacttagata ttcggggtgt tgatttacaa ggcgttaagt tagacagcta
     2401 ccaggcatcg ttgctcatgg agcggcttgg catcgctgtg attggttagt tcagcgttta
     2461 caccgggaga tgcgtttgac gatttacttt taaggggttc ctgtatctaa ttttttgctg
     2521 gacattcata cccgcgatca tcgcgtttat tgagtttatt atttatctat gcgattccga
     2581 tgaaaaattc gcacgaaaat atggctaact catagcaaaa aaaaagcccc ctaagggctt
     2641 ttttttgtac ctgacggtca gaccgattaa acataacatc ccttgcccgc acccccgcag
     2701 gcgccctcag cgcaatcctg ctgcatca
//
