LOCUS       NZ_CP081523             2810 bp    DNA     circular CON 20-OCT-2023
DEFINITION  Escherichia coli strain 20-MO00017 plasmid pQNR20-MO00017, complete
            sequence.
ACCESSION   NZ_CP081523
VERSION     NZ_CP081523.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN20835154
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2810)
  AUTHORS   Juraschek,K.A. and Hammerl,J.A.
  TITLE     qnrB-carrying ESBL
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2810)
  AUTHORS   Juraschek,K.A. and Hammerl,J.A.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-AUG-2021) Biological Safety, German Federal Institute
            for Risk Assessment, Diedersdorfer Weg 1, Berlin, Berlin 12277,
            Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SPAdes v. v3.14
            Genome Representation             :: Partial
            Expected Final Version            :: Yes
            Genome Coverage                   :: 30x
            Sequencing Technology             :: Illumina NextSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 10/20/2023 04:45:58
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4
            CDSs (total)                      :: 4
            Genes (coding)                    :: 2
            CDSs (with protein)               :: 2
            Genes (RNA)                       :: 0
            tRNAs                             :: 0
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP081523.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2810
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="20-MO00017"
                     /isolation_source="Pig, feces"
                     /db_xref="taxon:562"
                     /plasmid="pQNR20-MO00017"
                     /geo_loc_name="Germany"
                     /collection_date="2018"
     gene            <2..106
                     /locus_tag="K5589_RS00020"
                     /pseudo=""
     CDS             <2..106
                     /locus_tag="K5589_RS00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /name="replication initiation protein"
     gene            <361..516
                     /locus_tag="K5589_RS00005"
                     /old_locus_tag="K5589_00005"
                     /pseudo=""
     CDS             <361..516
                     /locus_tag="K5589_RS00005"
                     /old_locus_tag="K5589_00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707212.3"
                     /GO_function="GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /name="helix-turn-helix domain-containing protein"
     gene            610..1254
                     /locus_tag="K5589_RS00010"
                     /old_locus_tag="K5589_00010"
     CDS             610..1254
                     /locus_tag="K5589_RS00010"
                     /old_locus_tag="K5589_00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017900990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolone resistance pentapeptide repeat protein
                     QnrB19"
                     /protein_id="WP_012954666.1"
                     /translation="MTLALVGEKIDRNRFTGEKVENSTFFNCDFSGADLSGTEFIGCQF
                     YDRESQKGCNFSRAMLKDAIFKSCDLSMADFRNVSALGIEIRHCRAQGADFRGASFMNM
                     ITTRTWFCSAYITNTNLSYANFSKVVLEKCELWENRWMGTQVLGATFSGSDLSGGEFST
                     FDWRAANFTHCDLTNSELGDLDIRGVDLQGVKLDSYQASLLMERLGIAVIG"
                     /name="quinolone resistance pentapeptide repeat protein
                     QnrB19"
     gene            complement(1756..1992)
                     /locus_tag="K5589_RS00015"
                     /old_locus_tag="K5589_00015"
     CDS             complement(1756..1992)
                     /locus_tag="K5589_RS00015"
                     /old_locus_tag="K5589_00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001676031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_012772745.1"
                     /translation="MARLTNLTPAEKKFLDDAVAAAERASGKKLNQPNRHIVLNRARAQ
                     IELQRYADRQRALREDERQQSDFAWSRPRAPRR"
                     /name="hypothetical protein"
     ARG             611..1251
                     /name="qnrB19"
                     /gene="qnrB19"
                     /gene_name="quinolone resistance pentapeptide repeat
                     protein QnrB19"
                     /tool="amrfinderplus"
                     /drug_class="QUINOLONE"
                     /identity="100.0"
                     /coverage="100.0"
     Typing          complement(2158..2331)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="2.5600000000000002e-86"
     Typing          2..308
                     /name="rep_cluster_2358"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="80.299"
                     /coverage="99.0"
                     /evalue="7.04e-57"
     Typing          2310..2801
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="92.51"
                     /coverage="81.0"
                     /evalue="0.0"
ORIGIN
        1 accccccgtt cagtccgact gctgcgcctt atccggtaac tatcatcttg agtccaaccc
       61 ggaaagacac gacttatcgc cactggcagc agccactggt aactgattta gaggagttag
      121 tcttgaagtt atgcgcctgt taaggctaaa ctgaaagaac agatttggtt actgcgctcc
      181 tcgtaagcca gttaccacgg ttcaaagagt tgctgctctg agaaccttcg aaaaaccgcc
      241 ttgcagggcg gttttttcgt ttacagagca agagattacg acgccacaga aaggatctca
      301 agaagatcct tcaccgtaat ggaaatctat ccaatccgag cacataggat acacatcgtt
      361 ataacgctgg atctgcgtga attccagcag cagcaagaga aagactttct acaaaccagc
      421 ttacagcagg ctaaatttaa ccagaaaaaa gccgctgaat tactgggtct gacttatcat
      481 cagctacgtg ccttgctgaa gaaacaccag atctaatacg catttgcagg tgtcatactg
      541 gcgggtttga cgcataacgt cataaggttt accatggcgt cattactgta taaaaacaca
      601 ggcatagata tgactctggc attagttggc gaaaaaattg acagaaatcg cttcaccggt
      661 gagaaagttg aaaatagtac attttttaac tgcgattttt caggtgccga cctgagcggc
      721 actgaattta tcggctgcca gttctatgat cgcgaaagtc agaaagggtg caattttagt
      781 cgcgcaatgc tgaaagatgc cattttcaaa agctgtgatt tatcaatggc agatttccgc
      841 aacgtcagtg ccttgggcat tgaaattcgc cactgccgcg cacaaggcgc agatttccgc
      901 ggtgcaagct ttatgaatat gatcaccacg cgcacctggt tttgcagcgc atatatcact
      961 aatactaatc taagctacgc caatttttcg aaagtcgtgt tggaaaagtg tgagctgtgg
     1021 gaaaaccgct ggatggggac tcaggtactg ggtgcgacgt tcagtggttc agatctctcc
     1081 ggcggcgagt tttcgacttt cgactggcga gcagcaaact tcacacattg cgatctgacc
     1141 aattcggagt taggtgactt agatattcgg ggtgttgatt tacaaggcgt taagttagac
     1201 agctaccagg catcgttgct catggagcgg cttggcatcg ctgtgattgg ttagttcagc
     1261 gtttacaccg ggagatgcgt ttgacgattt acttttaacc agtacgactc ctggcaagca
     1321 ttgtgaaacg ttttctccag tggacgaata tcctgaccta ccgcatcaac gacatgccat
     1381 ctaatgctgc attctggaca ggaatttgat gaatatctaa ccaattcatt ttcgcgagaa
     1441 caacgaataa ccactcccga atcatgaagc catgtcttaa cgaaaatatg gctaactcat
     1501 agcaaaaaaa aagcccccta agggcttttt tttgtacctg acggtcagac cgattaaaca
     1561 taacatccct tgcccgcacc cccgcaggcg ccctcagcgc aatcctgctg catcagggcc
     1621 gtttccagca cctttcctca ccagtatccc ggaaaaacgc ccctgatgat ttctgcgcga
     1681 ctgcatggct atgcacgcag gtgaatggca ggtgatttcg cagcgtcagc accactaaac
     1741 cgccaaaagc caaaatcagc ggcgcggcgc gcgcggacgt gaccaggcaa aatcggactg
     1801 ctgccgctca tcctcacgta atgcacgctg gcggtcggcg taacgctgca actcaatttg
     1861 cgcacgcgct ctgttcagga cgatgtgccg gttaggctga tttagctttt tacctgacgc
     1921 tctttcagct gcggcaacag catcatcaag aaattttttc tctgcaggcg tcaagtttgt
     1981 cagtctggcc ataacgttct ccagtcagaa taaatcagca cagtctgcca gacacagtag
     2041 aaacgatgaa gcgccagcga cggtgaaagg cagtcagaaa cggtgaacgg gagcggattt
     2101 taatccggtg ccgtgaggcg ttggacaagc cgcaggcgcg tcattagctt ttagccggtt
     2161 tcggggcgca gccctgaacc agtcacgtag cgctagcgga gtgtatactg gctgactatg
     2221 ttggcacgga tgtggaaatc agtgaagtgc ttcatgtagc aggagaaaaa aggcggcacc
     2281 agcgcgtcag caggatatgt agtacaggat atattccgct tcctcgctca ctgactcgct
     2341 gcgctcggtc gttcggctgc ggcgagcggt gccggcttac aggcggggcg gaaatttcct
     2401 gggagatacc aggaaggagc ttaacaggga agagataagg ccgcggcgaa gccgtttttc
     2461 cataggctcc gcccccctga caaacatcat gaaatctgac gctcaaatca gtggtggcga
     2521 aacccgatgc ggcttaaaga taccaggcgt ttccccctgg tggctccctc gtgctccccc
     2581 gcattcctgc ctttcggttt accggtgtca ttccgctgtt atggccgcgt ttatctcatt
     2641 ccacgcctga cactcagttc cgcgaaggca gttcgctcct ggatggactg tatgcacgaa
     2701 ccccccgttc agtccgactg ctgcgcctta tccggtaact atcatcttga gtccaacccg
     2761 gaaagacacg acttatcgcc actggcagca gccactggta actgatttag
//
