LOCUS       NZ_CP081522             3100 bp    DNA     circular CON 20-OCT-2023
DEFINITION  Escherichia coli strain 20-MO00019 plasmid pQNR20-MO00019, complete
            sequence.
ACCESSION   NZ_CP081522
VERSION     NZ_CP081522.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN20835155
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3100)
  AUTHORS   Juraschek,K.A. and Hammerl,J.A.
  TITLE     qnrB-carrying ESBL
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3100)
  AUTHORS   Juraschek,K.A. and Hammerl,J.A.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-AUG-2021) Biological Safety, German Federal Institute
            for Risk Assessment, Diedersdorfer Weg 1, Berlin, Berlin 12277,
            Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SPAdes v. v3.14
            Genome Representation             :: Partial
            Expected Final Version            :: Yes
            Genome Coverage                   :: 30x
            Sequencing Technology             :: Illumina NextSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 10/20/2023 03:16:06
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3
            CDSs (total)                      :: 3
            Genes (coding)                    :: 2
            CDSs (with protein)               :: 2
            Genes (RNA)                       :: 0
            tRNAs                             :: 0
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP081522.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3100
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="20-MO00019"
                     /isolation_source="Pig, feces"
                     /db_xref="taxon:562"
                     /plasmid="pQNR20-MO00019"
                     /geo_loc_name="Germany"
                     /collection_date="2018"
     gene            508..657
                     /locus_tag="K5578_RS00005"
                     /old_locus_tag="K5578_00005"
     CDS             508..657
                     /locus_tag="K5578_RS00005"
                     /old_locus_tag="K5578_00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017880354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_162846302.1"
                     /translation="MPSEYDEQRGSTDTQLPGEYDLFDLMAKVTEEILHAPVDSGPPVG
                     REAL"
                     /name="hypothetical protein"
     gene            complement(1666..2310)
                     /locus_tag="K5578_RS00010"
                     /old_locus_tag="K5578_00010"
     CDS             complement(1666..2310)
                     /locus_tag="K5578_RS00010"
                     /old_locus_tag="K5578_00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017900990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolone resistance pentapeptide repeat protein
                     QnrB19"
                     /protein_id="WP_012954666.1"
                     /translation="MTLALVGEKIDRNRFTGEKVENSTFFNCDFSGADLSGTEFIGCQF
                     YDRESQKGCNFSRAMLKDAIFKSCDLSMADFRNVSALGIEIRHCRAQGADFRGASFMNM
                     ITTRTWFCSAYITNTNLSYANFSKVVLEKCELWENRWMGTQVLGATFSGSDLSGGEFST
                     FDWRAANFTHCDLTNSELGDLDIRGVDLQGVKLDSYQASLLMERLGIAVIG"
                     /name="quinolone resistance pentapeptide repeat protein
                     QnrB19"
     gene            complement(2404..>2559)
                     /locus_tag="K5578_RS00015"
                     /old_locus_tag="K5578_00015"
                     /pseudo=""
     CDS             complement(2404..>2559)
                     /locus_tag="K5578_RS00015"
                     /old_locus_tag="K5578_00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309907.2"
                     /GO_function="GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /name="helix-turn-helix domain-containing protein"
     ARG             complement(1670..2310)
                     /name="qnrB19"
                     /gene="qnrB19"
                     /gene_name="quinolone resistance pentapeptide repeat
                     protein QnrB19"
                     /tool="amrfinderplus"
                     /drug_class="QUINOLONE"
                     /identity="100.0"
                     /coverage="100.0"
     Typing          300..473
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="2.5600000000000002e-86"
     Typing          complement(2..179)
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="90.608"
                     /coverage="87.0"
                     /evalue="8.95e-60"
     Typing          complement(2613..3100)
                     /name="rep_cluster_2358"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="83.752"
                     /coverage="98.0"
                     /evalue="1.77e-127"
ORIGIN
        1 cgcggccata acagcggaat gacaccggta aaccgaaagg caggaatgcg ggggagcacg
       61 agggagccac cagggggaaa cgcctggtat ctttaagccg catcgggttt cgccaccact
      121 gatttgagcg tcagatttca tgatgtttgt caggggggcg gagcctatgg aaaaacggct
      181 tcgccgcggc cttatctctt ccctgttaag ctccttcctg gtatctccca ggaaatttcc
      241 gccccgcctg taagccggca ccgctcgccg cagccgaacg accgagcgca gcgagtcagt
      301 gagcgaggaa gcggaatata tcctgtacta catatcctgc tgacgcgctg gtgccgcctt
      361 ttttctcctg ctacatgaag cacttcactg atttccacat ccgtgccaac atagtcagcc
      421 agtatacact ccgctagcgc tacgtgactg gttcagggct gcgccccgaa acccgctaac
      481 aacctctctg ctactggata tcaccccatg ccatctgaat atgatgaaca acggggttcg
      541 actgatacgc agcttcccgg cgaatatgac ctttttgatt taatggcgaa ggttactgaa
      601 gagatcctgc atgcccctgt ggattctggg ccgcctgtcg gccgggaagc gttatagctt
      661 tttcgcttgc ccgcgtacgt ggtaatacgc cggtattacc ttttatttct ttattttcat
      721 gtaattacag tgggttttta atacctgggt attactcgtg aaaacgaaac attacttttt
      781 tttcttttaa ttcatgcagt tgcgatgaaa atcgcggctg cgttaggtgt atgaccattt
      841 tgaggttgaa aaataatcaa ccatggcggg tgatttacgc aggttaaggg gagatcgtga
      901 caaaaaaaag cgttcctcac tgtatcgttc atgcctgcat gaacgggtta ttcttcctga
      961 ctacagaatt acatcgctcc ttcagttcat acatcacttt tacaacactt ttacaacact
     1021 ccttcggcct gtacatcact tttacaacac ttttgcatca cttttacatc atttatacat
     1081 cacttttaca tcattccttc agctcatacg tcactttcat cctctgtgcc atttgtacat
     1141 cacccccttc ctgaacgtgc tgacagcggg ttttccggct gcgccggcaa cgcttcacgg
     1201 ccacgcaata aattgcgtcc ccgttcaccg ttttcggatg ggaagaacgc cggaaacagg
     1261 acttcccctg gctgaatggt gtgaatttcc gcgctatgca cttgcgcgca tactcatgca
     1321 tgccgtaaaa acagagcctg cgcgtttctg gcgggttttc gggtggtttg ttgcctgttt
     1381 taccggtttc ccgtcagaaa cgccctcaga gcctctcagg cggtgcacgc aagggatgtt
     1441 atgtttaatc ggtctgaccg tcaggtacaa aaaaaagccc ttagggggct ttttttttgc
     1501 tatgagttag ccatattttc gtgcgaattt ttcatcggaa tcgcatagat aaataataaa
     1561 ctcaataaac gcgatgatcg cgggtatgaa tgtccagcaa aaaattagat acaggaaccc
     1621 cttaaaagta aatcgtcaaa cgcatctccc ggtgtaaacg ctgaactaac caatcacagc
     1681 gatgccaagc cgctccatga gcaacgatgc ctggtagctg tctaacttaa cgccttgtaa
     1741 atcaacaccc cgaatatcta agtcacctaa ctccgaattg gtcagatcgc aatgtgtgaa
     1801 gtttgctgct cgccagtcga aagtcgaaaa ctcgccgccg gagagatctg aaccactgaa
     1861 cgtcgcaccc agtacctgag tccccatcca gcggttttcc cacagctcac acttttccaa
     1921 cacgactttc gaaaaattgg cgtagcttag attagtatta gtgatatatg cgctgcaaaa
     1981 ccaggtgcgc gtggtgatca tattcataaa gcttgcaccg cggaaatctg cgccttgtgc
     2041 gcggcagtgg cgaatttcaa tgcccaaggc actgacgttg cggaaatctg ccattgataa
     2101 atcacagctt ttgaaaatgg catctttcag cattgcgcga ctaaaattgc accctttctg
     2161 actttcgcga tcatagaact ggcagccgat aaattcagtg ccgctcaggt cggcacctga
     2221 aaaatcgcag ttaaaaaatg tactattttc aactttctca ccggtgaagc gatttctgtc
     2281 aattttttcg ccaactaatg ccagagtcat atctatgcct gtgtttttat acagtaatga
     2341 cgccatggta aaccttatga cgttatgcgt caaacccgcc agtatgacac ctgcaaatgc
     2401 gtattagatc tggtgtttct tcagcaaggc acgtagctga tgataagtca gacccagtaa
     2461 ttcagcggct tttttctggt taaatttagc ctgctgtaag ctggtttgta gaaagtcttt
     2521 ctcttgctgc tgctggaatt cacgcagatc cagcgttata acgatgtgta tcctatgtgc
     2581 tcggattgga tagatttcca ttacggtgaa ggatcttctt gagatccttt ctgtggcgtc
     2641 gtaatctctt gctctgtaaa cgaaaaaacc gccctgcaag gcggtttttc gaaggttctc
     2701 agagcagcaa ctctttgaac cgtggtaact ggcttacgag gagcgcagta accaaatctg
     2761 ttctttcagt ttagccttaa caggcgcata acttcaagac taactcctct aaatcagtta
     2821 ccagtggctg ctgccagtgg cgataagtcg tgtctttccg ggttggactc aagatgatag
     2881 ttaccggata aggcgcagca gtcggactga acggggggtt cgtgcataca gtccatccag
     2941 gagcgaactg ccttcgcgga actgagtgtc aggcgtggaa tgagataaac gcggccataa
     3001 cagcggaatg acaccggtaa accgaaaggc aggaatgcgg gggagcacga gggagccacc
     3061 agggggaaac gcctggtatc tttaagccgc atcgggtttc
//
