LOCUS       NZ_CP081521             2810 bp    DNA     circular CON 20-OCT-2023
DEFINITION  Escherichia coli strain 20-MO00028 plasmid pQNR20-MO00028, complete
            sequence.
ACCESSION   NZ_CP081521
VERSION     NZ_CP081521.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN20835156
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2810)
  AUTHORS   Juraschek,K.A. and Hammerl,J.A.
  TITLE     qnrB-carrying ESBL
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2810)
  AUTHORS   Juraschek,K.A. and Hammerl,J.A.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-AUG-2021) Biological Safety, German Federal Institute
            for Risk Assessment, Diedersdorfer Weg 1, Berlin, Berlin 12277,
            Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SPAdes v. v3.14
            Genome Representation             :: Partial
            Expected Final Version            :: Yes
            Genome Coverage                   :: 30x
            Sequencing Technology             :: Illumina NextSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 10/20/2023 03:06:46
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3
            CDSs (total)                      :: 3
            Genes (coding)                    :: 2
            CDSs (with protein)               :: 2
            Genes (RNA)                       :: 0
            tRNAs                             :: 0
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP081521.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2810
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="20-MO00028"
                     /isolation_source="Poultry, feces"
                     /db_xref="taxon:562"
                     /plasmid="pQNR20-MO00028"
                     /geo_loc_name="Germany"
                     /collection_date="2017"
     gene            207..443
                     /locus_tag="K5585_RS00005"
                     /old_locus_tag="K5585_00005"
     CDS             207..443
                     /locus_tag="K5585_RS00005"
                     /old_locus_tag="K5585_00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001676031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_012772745.1"
                     /translation="MARLTNLTPAEKKFLDDAVAAAERASGKKLNQPNRHIVLNRARAQ
                     IELQRYADRQRALREDERQQSDFAWSRPRAPRR"
                     /name="hypothetical protein"
     gene            complement(945..1589)
                     /locus_tag="K5585_RS00010"
                     /old_locus_tag="K5585_00010"
     CDS             complement(945..1589)
                     /locus_tag="K5585_RS00010"
                     /old_locus_tag="K5585_00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017900990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolone resistance pentapeptide repeat protein
                     QnrB19"
                     /protein_id="WP_012954666.1"
                     /translation="MTLALVGEKIDRNRFTGEKVENSTFFNCDFSGADLSGTEFIGCQF
                     YDRESQKGCNFSRAMLKDAIFKSCDLSMADFRNVSALGIEIRHCRAQGADFRGASFMNM
                     ITTRTWFCSAYITNTNLSYANFSKVVLEKCELWENRWMGTQVLGATFSGSDLSGGEFST
                     FDWRAANFTHCDLTNSELGDLDIRGVDLQGVKLDSYQASLLMERLGIAVIG"
                     /name="quinolone resistance pentapeptide repeat protein
                     QnrB19"
     gene            complement(1683..>1838)
                     /locus_tag="K5585_RS00015"
                     /old_locus_tag="K5585_00015"
                     /pseudo=""
     CDS             complement(1683..>1838)
                     /locus_tag="K5585_RS00015"
                     /old_locus_tag="K5585_00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309907.2"
                     /GO_function="GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /name="helix-turn-helix domain-containing protein"
     ARG             complement(949..1589)
                     /name="qnrB19"
                     /gene="qnrB19"
                     /gene_name="quinolone resistance pentapeptide repeat
                     protein QnrB19"
                     /tool="amrfinderplus"
                     /drug_class="QUINOLONE"
                     /identity="100.0"
                     /coverage="100.0"
     Typing          2568..2741
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="2.5600000000000002e-86"
     Typing          complement(2098..2589)
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="92.51"
                     /coverage="81.0"
                     /evalue="0.0"
ORIGIN
        1 cgctagcgct acgtgactgg ttcagggctg cgccccgaaa ccggctaaaa gctaatgacg
       61 cgcctgcggc ttgtccaacg cctcacggca ccggattaaa atccgctccc gttcaccgtt
      121 tctgactgcc tttcaccgtc gctggcgctt catcgtttct actgtgtctg gcagactgtg
      181 ctgatttatt ctgactggag aacgttatgg ccagactgac aaacttgacg cctgcagaga
      241 aaaaatttct tgatgatgct gttgccgcag ctgaaagagc gtcaggtaaa aagctaaatc
      301 agcctaaccg gcacatcgtc ctgaacagag cgcgtgcgca aattgagttg cagcgttacg
      361 ccgaccgcca gcgtgcatta cgtgaggatg agcggcagca gtccgatttt gcctggtcac
      421 gtccgcgcgc gccgcgccgc tgattttggc ttttggcggt ttagtggtgc tgacgctgcg
      481 aaatcacctg ccattcacct gcgtgcatag ccatgcagtc gcgcagaaat catcaggggc
      541 gtttttccgg gatactggtg aggaaaggtg ctggaaacgg ccctgatgca gcaggattgc
      601 gctgagggcg cctgcggggg tgcgggcaag ggatgttatg tttaatcggt ctgaccgtca
      661 ggtacaaaaa aaagccctta gggggctttt tttttgctat gagttagcca tattttcgtt
      721 aagacatggc ttcatgattc gggagtggtt attcgttgtt ctcgcgaaaa tgaattggtt
      781 agatattcat caaattcctg tccagaatgc agcattagat ggcatgtcgt tgatgcggta
      841 ggtcaggata ttcgtccact ggagaaaacg tttcacaatg cttgccagga gtcgtactgg
      901 ttaaaagtaa atcgtcaaac gcatctcccg gtgtaaacgc tgaactaacc aatcacagcg
      961 atgccaagcc gctccatgag caacgatgcc tggtagctgt ctaacttaac gccttgtaaa
     1021 tcaacacccc gaatatctaa gtcacctaac tccgaattgg tcagatcgca atgtgtgaag
     1081 tttgctgctc gccagtcgaa agtcgaaaac tcgccgccgg agagatctga accactgaac
     1141 gtcgcaccca gtacctgagt ccccatccag cggttttccc acagctcaca cttttccaac
     1201 acgactttcg aaaaattggc gtagcttaga ttagtattag tgatatatgc gctgcaaaac
     1261 caggtgcgcg tggtgatcat attcataaag cttgcaccgc ggaaatctgc gccttgtgcg
     1321 cggcagtggc gaatttcaat gcccaaggca ctgacgttgc ggaaatctgc cattgataaa
     1381 tcacagcttt tgaaaatggc atctttcagc attgcgcgac taaaattgca ccctttctga
     1441 ctttcgcgat catagaactg gcagccgata aattcagtgc cgctcaggtc ggcacctgaa
     1501 aaatcgcagt taaaaaatgt actattttca actttctcac cggtgaagcg atttctgtca
     1561 attttttcgc caactaatgc cagagtcata tctatgcctg tgtttttata cagtaatgac
     1621 gccatggtaa accttatgac gttatgcgtc aaacccgcca gtatgacacc tgcaaatgcg
     1681 tattagatct ggtgtttctt cagcaaggca cgtagctgat gataagtcag acccagtaat
     1741 tcagcggctt ttttctggtt aaatttagcc tgctgtaagc tggtttgtag aaagtctttc
     1801 tcttgctgct gctggaattc acgcagatcc agcgttataa cgatgtgtat cctatgtgct
     1861 cggattggat agatttccat tacggtgaag gatcttcttg agatcctttc tgtggcgtcg
     1921 taatctcttg ctctgtaaac gaaaaaaccg ccctgcaagg cggtttttcg aaggttctca
     1981 gagcagcaac tctttgaacc gtggtaactg gcttacgagg agcgcagtaa ccaaatctgt
     2041 tctttcagtt tagccttaac aggcgcataa cttcaagact aactcctcta aatcagttac
     2101 cagtggctgc tgccagtggc gataagtcgt gtctttccgg gttggactca agatgatagt
     2161 taccggataa ggcgcagcag tcggactgaa cggggggttc gtgcatacag tccatccagg
     2221 agcgaactgc cttcgcggaa ctgagtgtca ggcgtggaat gagataaacg cggccataac
     2281 agcggaatga caccggtaaa ccgaaaggca ggaatgcggg ggagcacgag ggagccacca
     2341 gggggaaacg cctggtatct ttaagccgca tcgggtttcg ccaccactga tttgagcgtc
     2401 agatttcatg atgtttgtca ggggggcgga gcctatggaa aaacggcttc gccgcggcct
     2461 tatctcttcc ctgttaagct ccttcctggt atctcccagg aaatttccgc cccgcctgta
     2521 agccggcacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc
     2581 ggaatatatc ctgtactaca tatcctgctg acgcgctggt gccgcctttt ttctcctgct
     2641 acatgaagca cttcactgat ttccacatcc gtgccaacat agtcagccag tatacactcc
     2701 gctagcgcta cgtgactggt tcagggctgc gccccgaaac cggctaaaag ctaatgacgc
     2761 gcctgcggct tgtccaacgc ctcacggcac cggattaaaa tccgctcccg
//
