LOCUS       NZ_CP081520             2810 bp    DNA     circular CON 20-OCT-2023
DEFINITION  Escherichia coli strain 20-MO00045 plasmid pQNR20-MO00045, complete
            sequence.
ACCESSION   NZ_CP081520
VERSION     NZ_CP081520.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN20835157
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2810)
  AUTHORS   Juraschek,K.A. and Hammerl,J.A.
  TITLE     qnrB-carrying ESBL
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2810)
  AUTHORS   Juraschek,K.A. and Hammerl,J.A.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-AUG-2021) Biological Safety, German Federal Institute
            for Risk Assessment, Diedersdorfer Weg 1, Berlin, Berlin 12277,
            Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SPAdes v. v3.14
            Genome Representation             :: Partial
            Expected Final Version            :: Yes
            Genome Coverage                   :: 30x
            Sequencing Technology             :: Illumina NextSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 10/20/2023 03:11:24
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3
            CDSs (total)                      :: 3
            Genes (coding)                    :: 2
            CDSs (with protein)               :: 2
            Genes (RNA)                       :: 0
            tRNAs                             :: 0
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP081520.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2810
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="20-MO00045"
                     /isolation_source="Poultry, feces"
                     /db_xref="taxon:562"
                     /plasmid="pQNR20-MO00045"
                     /geo_loc_name="Germany"
                     /collection_date="2017"
     gene            357..593
                     /locus_tag="K5599_RS00005"
                     /old_locus_tag="K5599_00005"
     CDS             357..593
                     /locus_tag="K5599_RS00005"
                     /old_locus_tag="K5599_00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001676031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_012772745.1"
                     /translation="MARLTNLTPAEKKFLDDAVAAAERASGKKLNQPNRHIVLNRARAQ
                     IELQRYADRQRALREDERQQSDFAWSRPRAPRR"
                     /name="hypothetical protein"
     gene            complement(1095..1739)
                     /locus_tag="K5599_RS00010"
                     /old_locus_tag="K5599_00010"
     CDS             complement(1095..1739)
                     /locus_tag="K5599_RS00010"
                     /old_locus_tag="K5599_00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017900990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolone resistance pentapeptide repeat protein
                     QnrB19"
                     /protein_id="WP_012954666.1"
                     /translation="MTLALVGEKIDRNRFTGEKVENSTFFNCDFSGADLSGTEFIGCQF
                     YDRESQKGCNFSRAMLKDAIFKSCDLSMADFRNVSALGIEIRHCRAQGADFRGASFMNM
                     ITTRTWFCSAYITNTNLSYANFSKVVLEKCELWENRWMGTQVLGATFSGSDLSGGEFST
                     FDWRAANFTHCDLTNSELGDLDIRGVDLQGVKLDSYQASLLMERLGIAVIG"
                     /name="quinolone resistance pentapeptide repeat protein
                     QnrB19"
     gene            complement(1833..>1988)
                     /locus_tag="K5599_RS00015"
                     /old_locus_tag="K5599_00015"
                     /pseudo=""
     CDS             complement(1833..>1988)
                     /locus_tag="K5599_RS00015"
                     /old_locus_tag="K5599_00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309907.2"
                     /GO_function="GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /name="helix-turn-helix domain-containing protein"
     ARG             complement(1099..1739)
                     /name="qnrB19"
                     /gene="qnrB19"
                     /gene_name="quinolone resistance pentapeptide repeat
                     protein QnrB19"
                     /tool="amrfinderplus"
                     /drug_class="QUINOLONE"
                     /identity="100.0"
                     /coverage="100.0"
     Typing          19..192
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="2.5600000000000002e-86"
     Typing          2718..2810
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="2.73e-41"
     Typing          complement(2248..2739)
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="92.51"
                     /coverage="81.0"
                     /evalue="0.0"
ORIGIN
        1 ccgagcgcag cgagtcagtg agcgaggaag cggaatatat cctgtactac atatcctgct
       61 gacgcgctgg tgccgccttt tttctcctgc tacatgaagc acttcactga tttccacatc
      121 cgtgccaaca tagtcagcca gtatacactc cgctagcgct acgtgactgg ttcagggctg
      181 cgccccgaaa ccggctaaaa gctaatgacg cgcctgcggc ttgtccaacg cctcacggca
      241 ccggattaaa atccgctccc gttcaccgtt tctgactgcc tttcaccgtc gctggcgctt
      301 catcgtttct actgtgtctg gcagactgtg ctgatttatt ctgactggag aacgttatgg
      361 ccagactgac aaacttgacg cctgcagaga aaaaatttct tgatgatgct gttgccgcag
      421 ctgaaagagc gtcaggtaaa aagctaaatc agcctaaccg gcacatcgtc ctgaacagag
      481 cgcgtgcgca aattgagttg cagcgttacg ccgaccgcca gcgtgcatta cgtgaggatg
      541 agcggcagca gtccgatttt gcctggtcac gtccgcgcgc gccgcgccgc tgattttggc
      601 ttttggcggt ttagtggtgc tgacgctgcg aaatcacctg ccattcacct gcgtgcatag
      661 ccatgcagtc gcgcagaaat catcaggggc gtttttccgg gatactggtg aggaaaggtg
      721 ctggaaacgg ccctgatgca gcaggattgc gctgagggcg cctgcggggg tgcgggcaag
      781 ggatgttatg tttaatcggt ctgaccgtca ggtacaaaaa aaagccctta gggggctttt
      841 tttttgctat gagttagcca tattttcgtt aagacatggc ttcatgattc gggagtggtt
      901 attcgttgtt ctcgcgaaaa tgaattggtt agatattcat caaattcctg tccagaatgc
      961 agcattagat ggcatgtcgt tgatgcggta ggtcaggata ttcgtccact ggagaaaacg
     1021 tttcacaatg cttgccagga gtcgtactgg ttaaaagtaa atcgtcaaac gcatctcccg
     1081 gtgtaaacgc tgaactaacc aatcacagcg atgccaagcc gctccatgag caacgatgcc
     1141 tggtagctgt ctaacttaac gccttgtaaa tcaacacccc gaatatctaa gtcacctaac
     1201 tccgaattgg tcagatcgca atgtgtgaag tttgctgctc gccagtcgaa agtcgaaaac
     1261 tcgccgccgg agagatctga accactgaac gtcgcaccca gtacctgagt ccccatccag
     1321 cggttttccc acagctcaca cttttccaac acgactttcg aaaaattggc gtagcttaga
     1381 ttagtattag tgatatatgc gctgcaaaac caggtgcgcg tggtgatcat attcataaag
     1441 cttgcaccgc ggaaatctgc gccttgtgcg cggcagtggc gaatttcaat gcccaaggca
     1501 ctgacgttgc ggaaatctgc cattgataaa tcacagcttt tgaaaatggc atctttcagc
     1561 attgcgcgac taaaattgca ccctttctga ctttcgcgat catagaactg gcagccgata
     1621 aattcagtgc cgctcaggtc ggcacctgaa aaatcgcagt taaaaaatgt actattttca
     1681 actttctcac cggtgaagcg atttctgtca attttttcgc caactaatgc cagagtcata
     1741 tctatgcctg tgtttttata cagtaatgac gccatggtaa accttatgac gttatgcgtc
     1801 aaacccgcca gtatgacacc tgcaaatgcg tattagatct ggtgtttctt cagcaaggca
     1861 cgtagctgat gataagtcag acccagtaat tcagcggctt ttttctggtt aaatttagcc
     1921 tgctgtaagc tggtttgtag aaagtctttc tcttgctgct gctggaattc acgcagatcc
     1981 agcgttataa cgatgtgtat cctatgtgct cggattggat agatttccat tacggtgaag
     2041 gatcttcttg agatcctttc tgtggcgtcg taatctcttg ctctgtaaac gaaaaaaccg
     2101 ccctgcaagg cggtttttcg aaggttctca gagcagcaac tctttgaacc gtggtaactg
     2161 gcttacgagg agcgcagtaa ccaaatctgt tctttcagtt tagccttaac aggcgcataa
     2221 cttcaagact aactcctcta aatcagttac cagtggctgc tgccagtggc gataagtcgt
     2281 gtctttccgg gttggactca agatgatagt taccggataa ggcgcagcag tcggactgaa
     2341 cggggggttc gtgcatacag tccatccagg agcgaactgc cttcgcggaa ctgagtgtca
     2401 ggcgtggaat gagataaacg cggccataac agcggaatga caccggtaaa ccgaaaggca
     2461 ggaatgcggg ggagcacgag ggagccacca gggggaaacg cctggtatct ttaagccgca
     2521 tcgggtttcg ccaccactga tttgagcgtc agatttcatg atgtttgtca ggggggcgga
     2581 gcctatggaa aaacggcttc gccgcggcct tatctcttcc ctgttaagct ccttcctggt
     2641 atctcccagg aaatttccgc cccgcctgta agccggcacc gctcgccgca gccgaacgac
     2701 cgagcgcagc gagtcagtga gcgaggaagc ggaatatatc ctgtactaca tatcctgctg
     2761 acgcgctggt gccgcctttt ttctcctgct acatgaagca cttcactgat
//
