LOCUS       NZ_CP081356             3126 bp    DNA     circular CON 29-SEP-2023
DEFINITION  Staphylococcus aureus strain SQL1 plasmid pla2MRSASQL1, complete
            sequence.
ACCESSION   NZ_CP081356
VERSION     NZ_CP081356.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN20550080
            Assembly: GCF_019738975.1
KEYWORDS    RefSeq.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3126)
  AUTHORS   Medrano,E.G. and Haydel,S.E.
  TITLE     Complete genome sequence of the methicillin-resistant Staphylococcus
            aureus SQL1 strain used for testing the antimicrobial properties of
            clay phyllosilicate minerals
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3126)
  AUTHORS   Medrano,E.G. and Haydel,S.E.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-AUG-2021) Insect Control and Cotton Disease Research,
            USDA-ARS, 2765 F&B Road, College Station, Texas 77845, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: FALCON-Unzip v. jan 2021
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 1823.0x
            Sequencing Technology             :: PacBio RSII
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 09/29/2023 07:02:42
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,927
            CDSs (total)                      :: 2,845
            Genes (coding)                    :: 2,762
            CDSs (with protein)               :: 2,762
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 42 of 83
            Pseudo Genes (incomplete)         :: 43 of 83
            Pseudo Genes (internal stop)      :: 17 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP081356.1.
            Bacteria and source DNA available from Shelley Haydel; Arizona
            State University Biodesign Institute  Center for Bioelectronics and
            Biosensors Tempe, AZ 85287-7501.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3126
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /strain="SQL1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /plasmid="pla2MRSASQL1"
                     /geo_loc_name="USA"
                     /lat_lon="33.42 N 111.93 W"
                     /collection_date="2021-01-01"
     gene            complement(166..747)
                     /locus_tag="K1X69_RS14590"
                     /old_locus_tag="K1X69_14585"
     CDS             complement(166..747)
                     /locus_tag="K1X69_RS14590"
                     /old_locus_tag="K1X69_14585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000991705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000991705.1"
                     /translation="MNAIQNSISLRKILVVSLSILTILSMVLDFNFKEAQAQNKNNIAD
                     KNVETLNEKEIEKELKYIYGKIIILDKDGTAKDVNLENAKSRYGYVPEGFQKLKNDIEN
                     KKKTSSISPRAVGGNYKNSGDCFYSEVLNSYGELLTGNIITAVFEDVKAKNLKGVAKKL
                     AKIGIKGNLAGIAATMVSKDVQCNLKYGLL"
                     /name="hypothetical protein"
     gene            1199..2194
                     /locus_tag="K1X69_RS14595"
                     /old_locus_tag="K1X69_14590"
     CDS             1199..2194
                     /locus_tag="K1X69_RS14595"
                     /old_locus_tag="K1X69_14590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="WP_001199892.1"
                     /translation="MQYNTTRSITENQDNKTLKDMTKSGKQRPWREKKIDNVSYADILE
                     ILKIKKAFNVKQCGNILEFKPTDEGYLKLHKTWFCKSKLCPVCNWRRAMKNSYQAQKVI
                     EKVIKEKPKARWLFLTLSTKNAIDGDTLEQSLKHLTKAFDRLSRYKKVKQNLVGFMRST
                     EVTVNKNDGSYNQHMHVLLCVENAYFRKKENYITQEEWVNLWQRALQVDYRPVANVKAI
                     KPNRKGDKDIESAIKETSKYSVKSSDFLTDDDEKNQEIVSDLEKGLYRKRMLSYGGLLK
                     QKHKILNLDDVEDGNLINASDEDKTTDEEEKAHSITAIWNFEKQNYYLRH"
                     /name="protein rep"
     gene            2777..2965
                     /locus_tag="K1X69_RS14600"
                     /old_locus_tag="K1X69_14595"
     CDS             2777..2965
                     /locus_tag="K1X69_RS14600"
                     /old_locus_tag="K1X69_14595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001039248.1"
                     /translation="MNKNTVLDEGYVIATILNVFFLLGLIFISHLENLYILIPYVILMG
                     INAIYLVVKVMNFKKNN"
                     /name="hypothetical protein"
     Typing          2265..2429
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="2.42e-81"
     Typing          1200..2194
                     /name="rep_cluster_1017"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.9"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 cgcatttatg ccgagaaaat ttattgatgt tgagaagaac ccttaactaa acttgcagac
       61 gaatgtcggc atagcgtgag ctattaagcc gaccattcga caagttttgg gattgttaag
      121 ggttccgagg ctcaacgtca ataaagcaat tggaataaat agtatctaaa gtaacccgta
      181 tttaagatta cattgtacat ccttcgaaac catagtagct gcgattccag ctaggtttcc
      241 ttttatccca attttagcta atttttttgc tacgcccttt aagtttttgg ctttaacatc
      301 ttcaaaaact gctgtgatta tgtttccagt taaaagttct ccatatgagt ttaaaacttc
      361 agaatagaaa cagtcaccgc tatttttgta gttcccacca actgctctag gtgatattga
      421 actagttttc tttttattct ctatgtcgtt ttttagtttt tggaatcctt caggaacata
      481 tccatatcga gatttggcat tttctagatt aacatcttta gctgttccgt ctttatctaa
      541 aataataatt ttgccatata tgtattttag ttctttttca atttcttttt cgtttaaagt
      601 ttcaacgttt ttatctgcaa tgttattttt gttttgggct tgagcttctt tgaaattaaa
      661 atccaagacc attgaaagta tggttaaaat acttaaagat actactaaaa tctttcttag
      721 tgatatactg ttttgaattg cattcattgt aaatgccccc ttgttttgtt atttaaatga
      781 attcaatata atattaacat ataaaaacaa aggagtttaa tatgaataag tttttttact
      841 ttttgattat tttattatct gtatttttaa tttttaattt tgttaattcg attttatttg
      901 ataatccttt tgacttttta gaaagtttta tttattcatt ggttttcaca ttaatttatt
      961 taggtttaga gggtcataaa aagaagaagg aataggttgt tttttgaaac gagtgtgaac
     1021 gagtttcttc ttgtcttgat actatataga aataactcga ttttatatat atagctgtaa
     1081 ctgttgatat tacagtgttt aaacgtgttt ttgtgcgtga aaggaaaatt tgacaataaa
     1141 aaaccccagt tatattatta aggtgtcgaa tcttaaataa tactgggggt ctttttatat
     1201 gcaatataat actactagaa gtataaccga aaatcaagat aataaaacgt taaaagatat
     1261 gacgaaaagt gggaaacaac gcccatggag agaaaagaaa atagataatg taagctatgc
     1321 agatatacta gaaattttaa aaatcaaaaa ggcttttaat gtaaaacaat gtggtaatat
     1381 tttagaattt aagccaactg atgaaggcta tttgaagtta cataagacat ggttttgtaa
     1441 atcaaaatta tgtccggttt gtaattggag acgtgctatg aaaaatagtt atcaagctca
     1501 aaaagtgatt gaaaaagtaa ttaaggaaaa gccaaaagca cgttggttgt ttttaacact
     1561 ttcaacaaaa aatgcgatag atggagatac tttagaacaa agtttgaagc atctaactaa
     1621 agcatttgat aggttgagta gatataaaaa ggttaaacaa aatcttgttg gatttatgcg
     1681 ttcaacagaa gttaccgtta ataaaaatga cggtagttat aatcagcaca tgcatgtttt
     1741 gttatgtgtt gaaaatgcat attttagaaa aaaagagaat tatataactc aagaagaatg
     1801 ggttaattta tggcaaagag cattacaagt tgactatcga cctgttgcta atgttaaagc
     1861 gatcaaaccg aatagaaaag gcgataaaga cattgaatcg gcaatcaaag agacctcaaa
     1921 atattcggtt aaatcatctg attttttaac tgatgatgat gaaaaaaatc aagaaattgt
     1981 aagtgattta gaaaaaggtt tgtatcgaaa acgtatgtta agttatggtg gattgcttaa
     2041 acaaaaacat aaaattttaa acttagacga tgtcgaagat ggtaatttga ttaatgcaag
     2101 tgatgaagat aaaacaacag acgaagaaga aaaagcacat tcaattaccg caatttggaa
     2161 tttcgaaaag caaaattatt atttaagaca ttagtgttga ctaatgtctt ttttgttgat
     2221 tttttataaa aaagtactgt cttatttttg tgacaaatgc tgtatgtagt gtcacaaaaa
     2281 taagacaaac gcaatatatt gtgtcacaaa aataagacag tacagctttg tatgatatca
     2341 ctttaaaata ccttaaaacc cctggaattt ctggctttgc cagacctatc atttttgaat
     2401 gatagcaaat tctccttatg ctcttacgga gtttttagag aaaaattaaa aattctcgat
     2461 ttttgataaa aaacgccctg caggaattta gaaaaacatg tggaagtttt aaaggatttt
     2521 atgctaattt ttaatttgca tgtaaatggg cagtgttaaa aaattagtca ctggttttgc
     2581 tgaaattttt gtttgtgaaa ttaccatata tttatttggc tcatatttgc gttttaagag
     2641 cttgtatata tttttaagtt aaaaacctat atgatacccc ctaaaccttt aaaatacccc
     2701 cttaaaactc aaaataaagg tatttataat ttgagtaagt cttgtgataa agttttttaa
     2761 aaaaggagtg tttattttga ataaaaacac agtgttagat gaaggttacg ttatagcaac
     2821 tattttaaat gttttctttt tacttggatt aatttttata tctcatttgg aaaatttata
     2881 tatattaatt ccgtatgtta ttttgatggg aataaatgct atttatttgg ttgttaaggt
     2941 tatgaatttc aaaaagaata attagaatat ttgaggggat tttgaaacga gtttcttctt
     3001 gttttcacac tgttttttta ttcctattgg tgttgttgct tactttttgt ttttctataa
     3061 agatgatgct tttgacgaga tggaagaaaa atatgattat catgaagata ataaaaaata
     3121 gacgac
//
