LOCUS       NZ_CP081197             4074 bp    DNA     circular CON 27-SEP-2023
DEFINITION  Escherichia coli strain EC1722-1 plasmid unnamed6, complete
            sequence.
ACCESSION   NZ_CP081197
VERSION     NZ_CP081197.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN20693749
            Assembly: GCF_019710415.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4074)
  AUTHORS   Zhu,X.
  TITLE     Whole genome of Escherichia coli ec1722-1
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4074)
  AUTHORS   Zhu,X.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-AUG-2021) Microbiology Laboratory, Hospital, 60 Wuning
            West Road, Dongyang, Zhejiang 322100, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: minimap v. 2.0; miniasm v. 2.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 300.0x
            Sequencing Technology :: Oxford Nanopore GridION; Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 09/27/2023 05:40:20
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,367
            CDSs (total)                      :: 5,240
            Genes (coding)                    :: 4,975
            CDSs (with protein)               :: 4,975
            Genes (RNA)                       :: 127
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 17
            Pseudo Genes (total)              :: 265
            CDSs (without protein)            :: 265
            Pseudo Genes (ambiguous residues) :: 0 of 265
            Pseudo Genes (frameshifted)       :: 107 of 265
            Pseudo Genes (incomplete)         :: 167 of 265
            Pseudo Genes (internal stop)      :: 48 of 265
            Pseudo Genes (multiple problems)  :: 54 of 265
            Pseudo Genes (short protein)      :: 4 of 265
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP081197.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4074
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="EC1722-1"
                     /isolation_source="Child feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="unnamed6"
                     /geo_loc_name="China:zhejiang"
                     /collection_date="2021-05-15"
     gene            62..316
                     /gene="mobC"
                     /locus_tag="K4F91_RS26595"
                     /old_locus_tag="K4F91_26595"
     CDS             62..316
                     /gene="mobC"
                     /locus_tag="K4F91_RS26595"
                     /old_locus_tag="K4F91_26595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase accessory protein MobC"
                     /protein_id="WP_000091744.1"
                     /translation="MSRTLEQKIADAEARLQRLKAKSRSLDTAQKVIVGAALLAKVRKP
                     EEVQLRAWLLQFLKAEVTRQADVTRILPLINELEALPEQ"
                     /name="mobC"
     gene            849..1550
                     /locus_tag="K4F91_RS26600"
                     /old_locus_tag="K4F91_26600"
     CDS             849..1550
                     /locus_tag="K4F91_RS26600"
                     /old_locus_tag="K4F91_26600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001281399.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_001281396.1"
                     /translation="MSDLIAYKSNALIEASYKLTLQEQRFLLVCISRLKSGEDSPSPDE
                     QKTMTITAAEFHDAFPDMGRQHAEARLKEAIDRLWDRSVIIKNEEKREEFRWVQYRAQY
                     FKGEAKVEITFSDAIMPYLTQLKGQFTRVVVKNVSSLSSTYSIRIYELLQQFRSTGDRT
                     IAIDDFRTMLAVEDKYQQFKDLNKMIIKPAIAELNEKSDLVVTVETIKKGRTVVALHFR
                     FKEDKQIKMTI"
                     /name="replication initiation protein"
     gene            complement(1792..3957)
                     /gene="mobQ"
                     /locus_tag="K4F91_RS26605"
                     /old_locus_tag="K4F91_26605"
     CDS             complement(1792..3957)
                     /gene="mobQ"
                     /locus_tag="K4F91_RS26605"
                     /old_locus_tag="K4F91_26605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobQ family relaxase"
                     /protein_id="WP_001029349.1"
                     /translation="MAIYHLSMKIISRSNGYSAVASAAYRSGSLMLDERTGLTHDYTRK
                     SGVAEAVILTPATAPAWCTNRAELWNAVEKAERRKNSQLAREIELAIPHELPQDAARET
                     VLAFVRENFVSQGMIADVAFHHMDKTNPHAHIMLTTRAVGPAGFGGKVRDWNDRTHAET
                     WRASWADHANRALANAGYQKEIDHRSYERQGLEKTPGIHLGKSACAMETRGIETERGEQ
                     NRLINRLNLEIQISRTELRNSGLTEPARRVADLLNIGLPDNATSYTLRDIIEALPKDSN
                     AAWKLTSNAMSMMADMQATRRRWEELNTERKEAMSHAASLKKSSPFSSGFSRIPLMQWA
                     APEYRSEQGKIQSLSQQMEKLRQHYRKVEKQDIPASQTAFEKQWQQWGESGLAALRAQL
                     LKREDELRRKELEDTERSRAERRAEQLRKNDNAVMDSGVLRAVVTGYGEAPMPGNGEMT
                     CYLLLHNRNGEYTLWGNELEKYRTRIFESVDLIRDRSGYICDRSEIGQHPPLQRIYSSA
                     TFEQLLTQVCQTWPQHTRNLRQPKTWPESFCLGEDRQPAMPSLAARKVDFTQGRLPPTL
                     MPVMSSVDRETRQLQLLLVMGVDDSLGGVVRLNGTLYPAFAVPSADNSQLVISALTDKG
                     LRYAGYGVAVNHDADSHISPAPELMEFHLKTREAPLFAVVNTPEKQPDHLFRSLGFNRT
                     WDEWRREEDARTHATERRPDRGWSQGM"
                     /name="mobQ"
     Typing          complement(3600..3958)
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="97.5"
                     /coverage="100.0"
                     /evalue="8.52e-174"
     Typing          3959..4074
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="4.5599999999999996e-54"
     Typing          850..1549
                     /name="rep_cluster_1778"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.287"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          complement(1802..4044)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="97.46"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 agtttttaaa ctaccagtga cgcgctaaaa tgcgattttt catcgcgcat ggagttacat
       61 catgagcaga acgctcgaac agaagattgc tgatgctgag gcgagattac agcgcctgaa
      121 ggcgaagagc cggagcctgg acacagcgca gaaggtcatt gtgggtgcag cattgctggc
      181 aaaggtcaga aagccggagg aagtgcagtt gcgagcctgg ttactccagt tcctgaaggc
      241 agaggtgaca cggcaggctg atgtgacgcg aatactgccg ctgattaacg agctggaggc
      301 gctgccagag cagtgattgt atcgtccgct cgccgcagcc cagcgaccga gcgttagcga
      361 gcgagtgggc gaggaagcgg aagtgaatca gtcgccatgt tgagcacata cgcacggttt
      421 ttcgacaggt gatatgcgct gaacccgcat gaatactgga tttccacagg gttggagggg
      481 gtgtattcgg aatcttcaat cctggttttt tgcaacacat tgtttttatt ggatttttat
      541 tttcagcacc ccaagttatc cacaggctcg ccgttaagtt tttaagatct tatttttaga
      601 tctttttttt taaaaccttt ttaggtcttg taaccacatg attttaaagg tgttctggcg
      661 agataggtgg agcttttggg ggtgataggt ggagcttttg ggggtgatag gtggagcttt
      721 tgggggtgat aagtggagct tttgggggtg taatccacag aaaaaagggg ttgcaaaaaa
      781 aaaacttcca ccaatatgat aggtggcgta cagtaaaaaa acgccaccaa tagcaacgag
      841 caagcgaaat gtcagatctg atagcttaca aatcaaacgc ccttatcgag gcaagctaca
      901 agctcacttt gcaggaacag cgcttcctgc tggtctgtat cagccgcctg aaatccggag
      961 aagattcccc ttcgccggat gaacaaaaaa ccatgaccat cacggctgca gagtttcatg
     1021 atgcctttcc tgacatggga cggcagcacg ctgaagctag gctcaaggaa gcgatagatc
     1081 gcctctggga caggtcagta atcatcaaga acgaggaaaa acgagaagag ttccgctggg
     1141 tgcagtaccg ggcgcagtat ttcaaaggtg aggcgaaagt agagatcacc ttctccgatg
     1201 ccatcatgcc gtatctgaca cagctcaagg ggcagtttac gcgggtagtc gtgaagaacg
     1261 tctccagcct ctccagcacc tacagcatcc gcatttacga gctgctccag cagttccgca
     1321 gcaccggtga cagaacgata gctatcgatg atttccgaac catgcttgcc gttgaagata
     1381 aatatcaaca attcaaagac ttgaataaga tgataatcaa gccagccatc gccgaactga
     1441 acgaaaaaag cgatctggtt gtaacggttg agacgatcaa aaaggggcgc acggttgtag
     1501 ccctgcactt tcgcttcaag gaagacaaac agatcaagat gacgatctag aatcgatttt
     1561 aaggcaccta aaacgctcac tgtcgcgttt ttcttgtgtc aggaatgcga aggtactatt
     1621 gaagtgaaaa gttgcgtcaa aaacgctctg atgcagcaaa aaaagaaccc ccaatttttg
     1681 ggggcggaaa aacaaaacta tgtttgcgct atgtgagtgt gcttcttgca ttcatccctg
     1741 tcgatgaaca tcgacgaaca taacacgatg ttaaacaaaa caaaatattg ttcacattcc
     1801 ctgtgaccaa ccgcgatcag gtctcctctc ggtggcgtgt gttcgtgcat cttcctcgcg
     1861 ccgccattcg tcccaggtac gattgaaacc cagcgagcgg aacagatgat ccggctgttt
     1921 ttctggtgtg ttgacgacag caaaaagcgg tgcctcacgg gttttcagat gaaactccat
     1981 aagttccggt gcaggtgata tgtggctgtc tgcatcatga tttacggcaa caccataccc
     2041 tgcataccgt aaccccttat cggtcagtgc actgataacc agctggctgt tatcggctga
     2101 gggaacggca aacgcgggat acagcgtacc attaagccgg acaacaccgc ccagagagtc
     2161 atccacgccc atgaccagca aaagctgaag ctggcgggtt tcccggtcga cagaactcat
     2221 gaccggcatc agcgtcggtg gcagacgtcc ctgcgtaaaa tccaccttcc ttgctgccag
     2281 tgatggcatg gctggctggc ggtcttctcc aagacagaaa gactccggcc atgtcttcgg
     2341 ctggcggaga ttgcgggtat gttgcggcca tgtctggcaa acctgcgtca gaagttgctc
     2401 aaaggtcgca gaggaataaa tacgttgcag tggtggatgt tgaccgattt cgcttcggtc
     2461 acagatataa ccactcctgt cacgtattaa atcaacactc tcaaagatcc tggtgcggta
     2521 tttctccagc tcgttccccc agagcgtgta ttctccgttc cggttgtgaa gcagaaggta
     2581 acaagtcatt tccccgttac caggcatggg agcctcgcca taccccgtaa cgactgctct
     2641 cagcacccct gaatccataa cggcattgtc atttttcctg agctgttccg ctcgtcgctc
     2701 cgcgcgacta cgttctgtat cctccagctc cttccggcgt aattcatctt cacgtttaag
     2761 caactgggct ctgagagctg caaggccgga ttccccccac tgctgccact gtttttcaaa
     2821 tgccgtctga ctcgcgggaa tgtcctgttt ctccaccttc cggtaatgct gtcgcaattt
     2881 ttccatttgc tggctcagag actggatttt tccctgctca ctacggtatt ccggtgcagc
     2941 ccattgcatc aggggaatac gggaaaaacc ggacgaaaac ggcgatgatt ttttcaggct
     3001 ggcagcatga ctcatcgctt cctttcgttc ggtgttcagc tcttcccagc gtctgcgcgt
     3061 cgcctgcata tctgccatca tcgacatggc gttactggtg agtttccatg ccgcgttact
     3121 gtccttcggc aacgcttcga tgatgtcacg tagtgtgtac gaagttgcgt tgtcaggaag
     3181 cccgatattc agaaggtcag ccacacggcg ggcaggctct gtcagcccgc tgttcctgag
     3241 ttcggtgcgg gaaatctgta tttccaggtt aagcctgtta atcagacgat tctgttcgcc
     3301 gcgttctgtt tcgataccgc gtgtttccat cgcacaggca gactttccga ggtgaatacc
     3361 cggcgttttc tcaagcccct gtcgctcgta tgaacgatgg tctatctctt tctgataacc
     3421 ggcgttcgcc agtgccctgt ttgcatggtc agcccatgat gcacgccacg tctcggcatg
     3481 tgtccggtcg ttccagtccc tgacctttcc gccgaacccc gcaggtccga cagccctcgt
     3541 ggtcagcata atgtgcgcat ggggatttgt tttatccatg tgatggaacg ccacatcagc
     3601 aatcatgccc tgactgacaa agttttctct gacaaaggcg agaacggttt cccgtgcggc
     3661 atcctgcggc agttcgtggg ggatggcgag ttcaatttcc cgcgcaagtt gtgagttctt
     3721 ccggcgttcg gccttctcaa cggcattcca gagttcggca cggttcgtac accaggcagg
     3781 ggctgtcgcg ggggtcagaa tcacagcctc agccacaccg cttttgcggg tgtagtcgtg
     3841 agtcagtccg gtacgctcat caagcattag tgaaccagaa cgataggcag cagaagcaac
     3901 ggcgctgtag ccgttgcttc tggagatgat tttcatactg agatgataaa tcgccatttc
     3961 tgccgtaatg cctttttgca gttttctttc agccccgcag ggcgcacaca atcgaaacgg
     4021 agtggagatt gtataagtgc gcacttcgtg atttgccgtg cattatgcca gctc
//
