LOCUS       NZ_CP081014             1919 bp    DNA     circular CON 22-SEP-2023
DEFINITION  Escherichia coli strain 25POL plasmid p25POL-D, complete sequence.
ACCESSION   NZ_CP081014
VERSION     NZ_CP081014.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN20701137
            Assembly: GCF_019645975.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1919)
  AUTHORS   Cao,G. and Zhong,C.
  TITLE     Complete genome of 25POL
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1919)
  AUTHORS   Cao,G. and Zhong,C.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-AUG-2021) College of Biomedical Sciences, Shandong
            First Medical University, 6699 Qingdao Road, Jinan 250117, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Canu v. 1.5
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 893.0x
            Sequencing Technology             :: Oxford Nanopore; BGISEQ
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 09/22/2023 05:38:20
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,068
            CDSs (total)                      :: 4,953
            Genes (coding)                    :: 4,722
            CDSs (with protein)               :: 4,722
            Genes (RNA)                       :: 115
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 100 of 231
            Pseudo Genes (incomplete)         :: 138 of 231
            Pseudo Genes (internal stop)      :: 54 of 231
            Pseudo Genes (multiple problems)  :: 53 of 231
            Pseudo Genes (short protein)      :: 4 of 231
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP081014.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1919
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="25POL"
                     /isolation_source="LAB"
                     /db_xref="taxon:562"
                     /plasmid="p25POL-D"
                     /geo_loc_name="China:Jinan"
                     /collection_date="2018-05"
     gene            62..244
                     /locus_tag="K3072_RS25125"
                     /old_locus_tag="K3072_25125"
     CDS             62..244
                     /locus_tag="K3072_RS25125"
                     /old_locus_tag="K3072_25125"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_016265984.1"
                     /translation="MLTFIGVVVVAVIAFKLFMLFFSSSESAVERATRKYMENPTSANY
                     KIMVATRMRNEKKRG"
                     /name="hypothetical protein"
     gene            1519..1755
                     /locus_tag="K3072_RS25130"
                     /old_locus_tag="K3072_25130"
     CDS             1519..1755
                     /locus_tag="K3072_RS25130"
                     /old_locus_tag="K3072_25130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001676031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001676031.1"
                     /translation="MNRLTNLTPAEKKFLDDAIAAAERASGKKLNQPNRHIVLNRARAQ
                     IESQRYADRQRALREDERQQSEFAWSRPRAPRR"
                     /name="hypothetical protein"
     Typing          1182..1355
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="98.857"
                     /coverage="100.0"
                     /evalue="5.54e-83"
     Typing          complement(694..1215)
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.308"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 gcgtttgagg cggtgcggcc aacggatgtt atgtttaatg ttgataacag gagtaagagt
       61 gatgcttaca tttattggtg tagtggtagt cgccgttatt gcatttaaat tatttatgct
      121 tttttttagt tcatccgaat ctgccgtcga acgtgctacc cgtaaatata tggagaaccc
      181 tacgagtgct aactataaaa taatggtggc tacacgcatg agaaatgaga agaaaagagg
      241 gtgatatatg tttgagttta ttggtttttt atttttgctt ttggtgtgtt atgtctttat
      301 acaaatggtt gctgtcaggg tgtttcctga atatggagta cggaaagcgg aggaacgata
      361 ccgtcgaaat ccatcagcag aaaatgagga tctgttatgg agagctagaa gtcgtataac
      421 taaacgcaag taatgtttcc taagtgtgtt gataggcaaa catggtgggg gttactactc
      481 tcggagttaa tgatcttctt gtgatcctgt cgtttcagtc gtaatctctt gctctgaaaa
      541 cgaaaaaacc acctggggag gtggtttgat cgaaggttaa gtcagttggg gaactgctta
      601 accaggtaac tgggtatacg agaccgcaga taccgaaact gttctttcag tgtagccgca
      661 gtcaggccca tacttcaaga cctctgaatc tctctctcgt atatcccagt taccagtggc
      721 tgctgccagt ggcgctttgt cgtgctttct cgggttggac tcaagatgat agttaccggg
      781 taaggcgcag cagtcggact gaacgggggg tttgtgcata cagtccagct tggagcgaac
      841 tgcctacccg gaactgagtg tcaggcgtgg aatgagataa acgcggccat aacagcggaa
      901 tgacaccggt aaaccgaaag gcaggaacag gagagcgcac gagggagccg ccagggggaa
      961 acgcctggta tctttatagt cctgtcgggt ttcgccacca ctgatttgag cgtcaggttt
     1021 cgtgatgctt gtcagggggg cggagcctat ggaaaaacgg cttcgccgcg gccctatcgc
     1081 ttccctgtta agtctcttcc tggcatcctc caggaaatct ccgccccgtt cgtaagccat
     1141 ttccgctcgc cgcagtcgaa cgaccgagcg tagcgagtca gtgagcgagg aagcggaata
     1201 tatcctgtat cacatattct gctgacgcgc cggtgccgcc ttttttctcc tgccacatga
     1261 agcacttcac tgacaccctc atccgtgcca acatagtaag ccagtataca ctccgctagc
     1321 gctgcgtgac tggttcaggg ctgcgccccg aaacccgcta aaaccactgc cgcgcctgcg
     1381 gcttgcccaa cgtctcacgt caccggatta aaatccgctc ccgttcaccg tttctggctg
     1441 tctttcagca ccgccagcgc ttcatcggtt ctgctgtgtc tggcagactg tgcggagtta
     1501 ttctgacggg agaatgctat gaacagactg acaaacctga cacctgcaga gaaaaaattt
     1561 cttgatgatg ctattgccgc agctgagaga gcgtcaggca aaaagctaaa tcagccgaac
     1621 cggcatatcg tcctgaacag agcgcgtgcg cagattgagt cgcagcgtta cgccgaccgc
     1681 cagcgtgcat tacgtgagga tgagcggcag cagtccgagt ttgcctggtc acgtccgcgc
     1741 gcgccgcgcc gctgattgcg ccttttggcg gtttagtggt gctgacactc cgaaatcgtc
     1801 tgccattcac ccgcgtgcat agccatgcag gtacgcagaa atcatcaggg gcgttttttc
     1861 tcgatatggg cacagaaagg tgtatgtttc aggccggagg cctcagaaac gcgctgagg
//
