LOCUS       NZ_CP081013             3173 bp    DNA     circular CON 22-SEP-2023
DEFINITION  Escherichia coli strain 25POL plasmid p25POL-C, complete sequence.
ACCESSION   NZ_CP081013
VERSION     NZ_CP081013.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN20701137
            Assembly: GCF_019645975.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3173)
  AUTHORS   Cao,G. and Zhong,C.
  TITLE     Complete genome of 25POL
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3173)
  AUTHORS   Cao,G. and Zhong,C.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-AUG-2021) College of Biomedical Sciences, Shandong
            First Medical University, 6699 Qingdao Road, Jinan 250117, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Canu v. 1.5
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 893.0x
            Sequencing Technology             :: Oxford Nanopore; BGISEQ
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 09/22/2023 05:38:20
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,068
            CDSs (total)                      :: 4,953
            Genes (coding)                    :: 4,722
            CDSs (with protein)               :: 4,722
            Genes (RNA)                       :: 115
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 100 of 231
            Pseudo Genes (incomplete)         :: 138 of 231
            Pseudo Genes (internal stop)      :: 54 of 231
            Pseudo Genes (multiple problems)  :: 53 of 231
            Pseudo Genes (short protein)      :: 4 of 231
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP081013.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3173
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="25POL"
                     /isolation_source="LAB"
                     /db_xref="taxon:562"
                     /plasmid="p25POL-C"
                     /geo_loc_name="China:Jinan"
                     /collection_date="2018-05"
     gene            complement(130..558)
                     /locus_tag="K3072_RS25100"
                     /old_locus_tag="K3072_25100"
     CDS             complement(130..558)
                     /locus_tag="K3072_RS25100"
                     /old_locus_tag="K3072_25100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000534558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000534558.1"
                     /translation="MGSGAGAQNPQLNIVSFSETFNLYYTSRYSAKTLDKIDDFIEHFE
                     KYNLFGDPLKGYPAWTGKVSPSWKVPDHYENKEAIEKYARANKLWHAHLGDPVFKDTFH
                     GKYKVSDWVIHFQRLTPNHIKLLELGYHDPMKLPQNFE"
                     /name="hypothetical protein"
     gene            complement(522..692)
                     /locus_tag="K3072_RS25105"
                     /old_locus_tag="K3072_25105"
     CDS             complement(522..692)
                     /locus_tag="K3072_RS25105"
                     /old_locus_tag="K3072_25105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001531327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001531327.1"
                     /translation="MKTEQRHDLVKDIEVFGVSLSLLISRANEKSVTMPSGLSREQRRA
                     WAAEQARKIHN"
                     /name="hypothetical protein"
     gene            850..1278
                     /locus_tag="K3072_RS25110"
                     /old_locus_tag="K3072_25110"
     CDS             850..1278
                     /locus_tag="K3072_RS25110"
                     /old_locus_tag="K3072_25110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000222531.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_000222531.1"
                     /translation="MTYSVKNKWKAGGLSLGWTAVPTVLFFIQNEKKLTSVAFNTLLNL
                     IVHWWSLQEWPHPSMESLAIRMGVSVRTVQRAINDLEKANLLDKKPTSKSDRRYGGRNI
                     YDLTKLVDYLDTMGPSVAEQVKKPRHKKPVYTVRKTTA"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(1667..1990)
                     /gene="mobA"
                     /locus_tag="K3072_RS25115"
                     /old_locus_tag="K3072_25115"
     CDS             complement(1667..1990)
                     /gene="mobA"
                     /locus_tag="K3072_RS25115"
                     /old_locus_tag="K3072_25115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052604.1"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization protein MobA"
                     /protein_id="WP_000166966.1"
                     /translation="MTKRSGSNTRRRAISRPVRLTAEEDQEIRKRAAECGKTVSGFLRA
                     AALGKKVNSLTDDRVLKEVMRLGALQKKLFIDGKRVGDREYAEVLIAITEYHRALLSRL
                     MAD"
                     /name="mobA"
     gene            2274..2468
                     /locus_tag="K3072_RS25120"
                     /old_locus_tag="K3072_25120"
     CDS             2274..2468
                     /locus_tag="K3072_RS25120"
                     /old_locus_tag="K3072_25120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_001041622.1"
                     /translation="MNKQQQAVLNMAGFIKSQSLTLLEKLDALNADEQAAMCEKLHELA
                     EELQNSIQTRFEAENLTGI"
                     /name="Rop family plasmid primer RNA-binding protein"
     Typing          complement(2000..2236)
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="3.49e-121"
     Typing          2504..3028
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.085"
                     /coverage="95.0"
                     /evalue="0.0"
     Typing          complement(1671..1990)
                     /name="MOBF"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.065"
                     /coverage="100.0"
                     /evalue="2.23e-62"
ORIGIN
        1 gttagagaat ctgccaggat taactaacct tcgaaaaacc acctgccagg gtggtttttt
       61 cgtttttaca atagtgagat tacgacgaga ctgaatgaat ctcaagattt catttagcct
      121 gccgctcgat cattcaaagt tttgaggtag cttcattggg tcatggtatc caagctctaa
      181 aagctttata tggttcggtg tcagccgctg gaaatgaata acccagtcag aaaccttgta
      241 tttcccatga aacgtatctt taaaaaccgg atcgcctaaa tgagcatgcc ataatttgtt
      301 agctctagca tacttctcaa tagcttcttt gttttcgtaa tgatcaggca ctttccacga
      361 tggcgatact ttgccagtcc aagctggata tccttttaaa ggatcgccaa agagattgta
      421 tttttcaaaa tgctcaatga aatcatcgat tttatcgagt gtttttgcag aatatctgga
      481 tgtgtaataa aggttgaagg tctcagagaa tgagacaata ttcaattgtg gattttgcgc
      541 gcctgctccg ctgcccatgc tcttctctgc tcccgactta gaccagatgg cattgtaaca
      601 gacttctcat tcgctctgga aatcaacaga gacaaggata cgccaaaaac ctcaatatct
      661 ttaacaagat catgacgttg ttcggttttc atatcgctaa ctccgtatct aattcaggca
      721 ggaaaaaatc ttttcctcgt tttcagagta gtcttttttg gttgcacaat aatggcgcca
      781 gtgtcaccct attcatatga atataccata taatatatac tttaaatatt ttggggctta
      841 ggaaaatcaa tgacttactc cgttaagaat aaatggaaag ctggcggttt gtctcttggc
      901 tggacagcag tgcctaccgt attgtttttt atacaaaacg agaaaaagct aacttcagtg
      961 gcctttaaca cactacttaa cctgatcgtt cactggtgga gtttgcaaga gtggccgcat
     1021 ccgtcaatgg aaagtctagc catacgtatg ggggtttctg tacggactgt tcagagagct
     1081 atcaatgatc ttgaaaaagc taatctcttg gacaagaaac caacatcgaa gagtgatcgc
     1141 cgatatggtg gtcgcaatat ttatgatcta acaaagctag ttgactacct agatacaatg
     1201 ggtccatcag tagctgaaca agttaagaaa ccacgccata aaaaaccggt atataccgta
     1261 agaaaaacga cagcttaaaa aagcacaatg gaactgagcg cgagcgatgc acgatagcaa
     1321 aacgatatat tacttattgc attggtcttg gtagccctgc ttctggctaa accactgtca
     1381 ggagtaattg gctttgcctg taatgggttc atcgcttcac agttaaagtt atcgcatagc
     1441 tcatatattt accataatcc cttaattgta cgcaccactg aaacgcgctg aacgcgattc
     1501 tgacgcagac accgcaaaaa gagatgtttt accccggaaa aacaggaagg atgcgtcagg
     1561 atcgttttca gctcgctgca tagctatgca tgaaagcgaa tggtgatcac tttgggagct
     1621 tacggtgttc ataccgtcag ttttcgacag ttttctctcc gggaagctaa tctgccataa
     1681 gcctggataa cagggcacgg tgatactccg taatagcgat cagcacctcc gcatactccc
     1741 tgtccccgac acgcttgccg tcgataaaga gttttttctg taacgccccc agtctcataa
     1801 cttctttcag tacccgatca tcagtcagtg agttaacttt cttaccgaga gctgccgccc
     1861 gtaaaaaacc ggaaacggtc ttgccgcatt cagcagccct ttttctgatt tcctggtctt
     1921 cttctgccgt cagacgaaca gggcgactga tagcccgcct gcgcgtatta cttccacttc
     1981 gttttgtcat aaaacatggc tccgtatctg acatgggtgt cggggcaaag ccctgaccag
     2041 ggcaattgta atagcgtgca tgtatgcgcg gtataacaat tgcacatcct gtcctgtcag
     2101 caagctcgaa ttaccgacaa aggcacggca gttttttggc agtgacagtg ttgtctgaca
     2161 gaaatgcccg tcaaaaagcc aagcggcaga atagcggcag caaaacagtc gaaaagtggt
     2221 acgcgatata atcacacaac gcagatgaca gacagaacgg agaaaacgaa aagatgaata
     2281 agcagcagca agccgtactc aacatggcgg gattcataaa aagtcagagc ctgacgctgc
     2341 tcgaaaaact ggacgcactc aatgccgacg aacaggccgc catgtgtgag aaactgcacg
     2401 aactcgcaga agaactccag aacagcatac agacacgctt tgaagcggaa aatcttaccg
     2461 ggatataacg catccggaca ggatgcagaa cggcatagag gagcgtaagg agaaaatgtc
     2521 acataaggcg ctcttccgct tcctcgctca ctgactcgct acgctcggtc gttcgactgc
     2581 ggcgagcggt gtcagctcac tcaaaggcgg taatacggtt atccacagaa tcgggggata
     2641 aaaccggaaa gaacatgtga gcaaaagaca aagacccaga agaagggcgc gcgggaggcg
     2701 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
     2761 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
     2821 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
     2881 agcgtggcgc tttctcatag ctcacgctgt tggtatctcg gttcggtgta ggtcgttcgc
     2941 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
     3001 aactatcgtc ttgagtccaa cccggtaaga cacgacttaa cgccactggc aacggccact
     3061 ggtaagcagg atgtgcgaga gatgtaacga gagttcttga agtggcggcc taactgaggc
     3121 tacactgaaa ggacagtatt tggtgacatg tctcgcacaa gactgttacc acg
//
