LOCUS       NZ_CP080643             2621 bp    DNA     circular CON 31-MAR-2024
DEFINITION  Acinetobacter lwoffii strain EK30A plasmid pALWEK1.16, complete
            sequence.
ACCESSION   NZ_CP080643
VERSION     NZ_CP080643.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN05251105
            Assembly: GCF_019787625.1
KEYWORDS    RefSeq.
SOURCE      Acinetobacter lwoffii
  ORGANISM  Acinetobacter lwoffii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Acinetobacter.
REFERENCE   1  (bases 1 to 2621)
  AUTHORS   Mindlin,S., Beletsky,A., Rakitin,A., Mardanov,A. and Petrova,M.
  TITLE     Acinetobacter Plasmids: Diversity and Development of Classification
            Strategies
  JOURNAL   Front Microbiol 11, 588410 (2020)
   PUBMED   33304332
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2621)
  AUTHORS   Rakitin,A.L., Ermakova,A.Y., Beletsky,A.V., Petrova,M.,
            Mardanov,A.V. and Ravin,N.V.
  TITLE     Genome analysis of Acinetobacter lwoffii strains isolated from
            permafrost soils aged from 15 thousand to 1.8 million years revealed
            their close relationships with present-day clinical isolates
  JOURNAL   Unpublished
REFERENCE   3  (bases 1 to 2621)
  AUTHORS   Rakitin,A.L., Ermakova,A.Y., Beletsky,A.V., Petrova,M.,
            Mardanov,A.V. and Ravin,N.V.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2021) Institute of Bioengineering, Research Center
            of Biotechnology of the Russian Academy of Sciences, Moscow 119071,
            Russia
REFERENCE   4  (bases 1 to 2621)
  AUTHORS   Mindlin,S.Z., Beletsky,A.V., Mardanov,A.V. and Petrova,M.A.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2020) Institute of Bioengineering, Research Center
            of Biotechnology of the Russian Academy of Sciences, Moscow 119071,
            Russia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.8-beta
            Genome Coverage                   :: 158.0x
            Sequencing Technology             :: Illumina; Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_019787625.1-RS_2024_03_31
            Annotation Date                   :: 03/31/2024 06:12:30
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,448
            CDSs (total)                      :: 3,336
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 112
            rRNAs                             :: 7, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 222
            CDSs (without protein)            :: 222
            Pseudo Genes (ambiguous residues) :: 0 of 222
            Pseudo Genes (frameshifted)       :: 80 of 222
            Pseudo Genes (incomplete)         :: 138 of 222
            Pseudo Genes (internal stop)      :: 46 of 222
            Pseudo Genes (multiple problems)  :: 39 of 222
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP080643.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2621
                     /organism="Acinetobacter lwoffii"
                     /mol_type="genomic DNA"
                     /strain="EK30A"
                     /isolation_source="permafrost, Kolyma lowland"
                     /db_xref="taxon:28090"
                     /plasmid="pALWEK1.16"
                     /geo_loc_name="Russia"
     gene            complement(25..360)
                     /locus_tag="ABEKA_RS17110"
                     /old_locus_tag="ABEKA_3588"
     CDS             complement(25..360)
                     /locus_tag="ABEKA_RS17110"
                     /old_locus_tag="ABEKA_3588"
                     /inference="COORDINATES: protein motif:HMM:NF018862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1413 domain-containing protein"
                     /protein_id="WP_121980414.1"
                     /translation="MNVSLTLPESIVDWANTQEDIQKAILDVLEKHVLSLNPATINAIK
                     LLKKNSVILPKGMEFQIQQIIGTDGWNALNRSERLSLGRKVKSSPNTYGLEFARTNTAN
                     HALYIRM"
                     /name="DUF1413 domain-containing protein"
     gene            587..883
                     /locus_tag="ABEKA_RS17115"
                     /old_locus_tag="ABEKA_3589"
     CDS             587..883
                     /locus_tag="ABEKA_RS17115"
                     /old_locus_tag="ABEKA_3589"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_121980413.1"
                     /translation="MTNPFTDLLNIATHLLTKDLREKQKREEEEMAVLALKENLINFSL
                     TCRCSGTAIPIGDTVNRYICLECGTRFVHAKHQFPSYYLSNELRNEIIILTKK"
                     /name="hypothetical protein"
     gene            1569..2543
                     /gene="repM"
                     /locus_tag="ABEKA_RS17120"
                     /old_locus_tag="ABEKA_3590"
     CDS             1569..2543
                     /gene="repM"
                     /locus_tag="ABEKA_RS17120"
                     /old_locus_tag="ABEKA_3590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004846161.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein RepM"
                     /protein_id="WP_127262086.1"
                     /translation="MSDLIVKDNALMNASYNLDLVEQRLILLAILEARESGKGINANDP
                     LTVHAESYINQFGVARQTAYQALKDACKDLFARQFSYQEKRERGRANITSRWVSQIAYI
                     DETATVEVIFAPAVVPLITRLEEQFTQYDIEQISGLSSAYAVRLYELLICWRSTGKTPV
                     IELAEFRKRLGVLNDEYTRSDNFKKWIIENPIKQINEHTDITASYEQHKKGRTITGFSF
                     KFKQKKKTEPETPKNSDSSQRIEKPSQIPANIVKQPENANLSDLQHRASKITGLIMSNR
                     LSDRFKQGDESIMQMMARIQSEITTDSIAEQWQSKLEEFGVVF"
                     /name="repM"
ORIGIN
        1 aagtgacgct ttggcatctt aaaactacat gcgtatgtaa agagcatgat ttgctgtgtt
       61 tgttcgggca aactccaagc cgtatgtatt cggtgagctc ttaacttttc tgcctaaact
      121 taaccgttct gaacggttta atgcattcca gccatcagta cctattatct gctgaatttg
      181 aaattccatt ccctttggaa gtatgactga attttttttc agtaatttta ttgcattgat
      241 tgttgctggg tttaggctaa gcacatgctt ttcaagtaca tccaatattg ctttttgaat
      301 atcttcttga gtattagccc aatccacaat cgactcaggt agtgttaaag acacattcat
      361 atttatttaa cccaaaataa ttattaattt aataaaaaat ataatataca ccaaatatta
      421 tttcaatata aatatttgtg ctcggctaaa ttttgttgtt ttctgattct tcaatccaag
      481 actataaaat ttacatgatt aaagaaaatc cttaaaaatt agtgtggttt attgtacttt
      541 tatttaattt ttgatctaaa attcttattt taattagaat attgtgatga ctaatccatt
      601 tacagattta ctaaacatag caacccatct tcttactaaa gatttaagag agaaacaaaa
      661 gcgagaagaa gaagaaatgg ctgtattagc actaaaagaa aatttaataa attttagttt
      721 aacgtgcaga tgttcaggta ctgctattcc tattggcgat acagtaaaca gatatatttg
      781 tttagaatgt ggaactagat ttgttcatgc aaagcatcaa tttccatcat attatttaag
      841 taatgagtta agaaacgaaa ttattattct tacaaaaaaa taagcgtcac tactttaagt
      901 gacacttttt tgtgtttcaa aaaattaatt agcgagtgtc tacgagcgac tcaatgaaag
      961 ttcgattatc tttctccctg aaaaactgct ttaaaaaaat ggaaccgtga tggtttttac
     1021 tatcgtgagc ttttactttt caaaaaatac gagcgaagcg agttcctagt cgcttttgat
     1081 ttgccctaac taacggccca tttttaatag gccgttagtt agggcagttg ttggggtttc
     1141 ggggctcgcc ctgaacaagg tcacagagtg ggaatttatc acggcagtga aaaagtaacc
     1201 tttgtgtatc cttgctctat gctttttaaa atctgttttt tctgcctttt ctccgtcatt
     1261 tgctaaaaat tgcccctgac gtaacgaagt gaagtcaaaa aagtttgagc tttagcgaaa
     1321 accaagggca attttcataa tgtaaatggg cttttaaagt ttttaaatgc ttttaaatgc
     1381 ttttagacat gctgaaatgc ccacacagca aggcatacag aggacatact cctaggttta
     1441 cctaccaata ctcctaggtt tacctaccaa tactcctagg tttacctacc aatactccta
     1501 ggtttacctt gcataatcct agaaaaaata atacaaagga attactagat ttaatattaa
     1561 taagtaaaat gagtgattta atagtaaaag ataacgcctt aatgaacgct agttataacc
     1621 tagacttagt tgagcagcgt ttaattctct tggctatcct tgaggctaga gaatcaggca
     1681 aaggaatcaa tgctaatgat cctctaaccg ttcatgctga aagctatatc aatcaatttg
     1741 gtgtagctag acagactgca tatcaagcat taaaagatgc ttgtaaggac ttatttgcac
     1801 gccaatttag ttaccaagaa aaacgtgagc gtggccgagc taatattaca agtcgttggg
     1861 tatcgcaaat tgcttacatt gatgaaacgg caacagttga ggttattttt gcccctgccg
     1921 ttgttccact gattacacga ttagaggaac aattcaccca atacgatatt gaacaaatta
     1981 gcggtttatc gagtgcctat gccgtccgtc tatatgaatt attgatttgt tggcgtagta
     2041 cagggaaaac gcctgttatt gaactagcag agtttagaaa gcgtttaggt gttttaaatg
     2101 atgaatatac tagatcggat aatttcaaaa aatggattat tgaaaatcct attaaacaaa
     2161 taaacgagca taccgacatc acagcaagct acgaacagca caaaaaaggg cggacaatca
     2221 cagggttttc attcaagttt aaacagaaga aaaaaacaga acctgaaacg ccaaaaaata
     2281 gcgattctag ccaacgtatc gaaaagccta gtcaaatccc tgccaacatc gtaaaacaac
     2341 ctgaaaacgc aaatttgagc gatttacagc atcgagcaag caagattaca gggttaatca
     2401 tgtctaaccg tctatcagat cggtttaaac aaggtgatga atcaatcatg cagatgatgg
     2461 cacgtattca aagtgagatt acaaccgatt caatagccga gcaatggcaa tcaaaattag
     2521 aagaatttgg tgtggttttc tagtctgtca cgtggcaatc acgtagaaaa ttatgtttga
     2581 aaggcttact gttattacat ttcaaaaagc gtcactactt t
//
