LOCUS       NZ_CP080642             4677 bp    DNA     circular CON 31-MAR-2024
DEFINITION  Acinetobacter lwoffii strain EK30A plasmid pALWEK1.15, complete
            sequence.
ACCESSION   NZ_CP080642
VERSION     NZ_CP080642.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN05251105
            Assembly: GCF_019787625.1
KEYWORDS    RefSeq.
SOURCE      Acinetobacter lwoffii
  ORGANISM  Acinetobacter lwoffii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Acinetobacter.
REFERENCE   1  (bases 1 to 4677)
  AUTHORS   Mindlin,S., Beletsky,A., Rakitin,A., Mardanov,A. and Petrova,M.
  TITLE     Acinetobacter Plasmids: Diversity and Development of Classification
            Strategies
  JOURNAL   Front Microbiol 11, 588410 (2020)
   PUBMED   33304332
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4677)
  AUTHORS   Rakitin,A.L., Ermakova,A.Y., Beletsky,A.V., Petrova,M.,
            Mardanov,A.V. and Ravin,N.V.
  TITLE     Genome analysis of Acinetobacter lwoffii strains isolated from
            permafrost soils aged from 15 thousand to 1.8 million years revealed
            their close relationships with present-day clinical isolates
  JOURNAL   Unpublished
REFERENCE   3  (bases 1 to 4677)
  AUTHORS   Rakitin,A.L., Ermakova,A.Y., Beletsky,A.V., Petrova,M.,
            Mardanov,A.V. and Ravin,N.V.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2021) Institute of Bioengineering, Research Center
            of Biotechnology of the Russian Academy of Sciences, Moscow 119071,
            Russia
REFERENCE   4  (bases 1 to 4677)
  AUTHORS   Mindlin,S.Z., Beletsky,A.V., Mardanov,A.V. and Petrova,M.A.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2020) Institute of Bioengineering, Research Center
            of Biotechnology of the Russian Academy of Sciences, Moscow 119071,
            Russia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.8-beta
            Genome Coverage                   :: 158.0x
            Sequencing Technology             :: Illumina; Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_019787625.1-RS_2024_03_31
            Annotation Date                   :: 03/31/2024 06:12:30
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,448
            CDSs (total)                      :: 3,336
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 112
            rRNAs                             :: 7, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 222
            CDSs (without protein)            :: 222
            Pseudo Genes (ambiguous residues) :: 0 of 222
            Pseudo Genes (frameshifted)       :: 80 of 222
            Pseudo Genes (incomplete)         :: 138 of 222
            Pseudo Genes (internal stop)      :: 46 of 222
            Pseudo Genes (multiple problems)  :: 39 of 222
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP080642.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4677
                     /organism="Acinetobacter lwoffii"
                     /mol_type="genomic DNA"
                     /strain="EK30A"
                     /isolation_source="permafrost, Kolyma lowland"
                     /db_xref="taxon:28090"
                     /plasmid="pALWEK1.15"
                     /geo_loc_name="Russia"
     gene            join(4369..4677,1..621)
                     /locus_tag="ABEKA_RS17080"
     CDS             join(4369..4677,1..621)
                     /locus_tag="ABEKA_RS17080"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="WP_004282296.1"
                     /translation="MAIARLSVKVGKAGKAAPHAEYIDRDEEKKLKQEQAETDLEHSAY
                     GNMPKWAEHNPITFWQAADLYERKNGSTYREYEIALPREMNAEQRLELVEDFIQSEIGS
                     KYPYQFAIHNPKAMDGKDQPHVHLMFNERLQDGIERDPEQYFKRYNAKNPERGGAKKDN
                     TGKGYQERKTDIKDLRQRWADLCNSHLEKHQIDSRIDMRSYKEQGIEKEPEKKLLPSQA
                     KDPEIREALQQSRIAYKELEQLDLGDPKKDLKDLKNSPISDKEIKQGIESFKADFDSFK
                     QLALEQYKQQQKLEREQQKTMKFRGMSR"
                     /name="MobA/MobL family protein"
     gene            618..1118
                     /locus_tag="ABEKA_RS17085"
                     /old_locus_tag="ABEKA_3582"
     CDS             618..1118
                     /locus_tag="ABEKA_RS17085"
                     /old_locus_tag="ABEKA_3582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018680098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_005253924.1"
                     /translation="MTPDEKKLYALMAVAEQQQNLVNQAVKELQITKEHIQSTIAGQTH
                     HTISKAVNEGLQDGTAELIKTAKALGRLKNSIESASDQFSWKLITTFLGVFSVFILTMT
                     WLFASYIKPLENIPKIESLKQQGIQFDIQSCAVGEESKPCVRVMKEQCGYGKHGDLCVI
                     DPK"
                     /name="hypothetical protein"
     gene            1200..2015
                     /locus_tag="ABEKA_RS17090"
                     /old_locus_tag="ABEKA_3583"
     CDS             1200..2015
                     /locus_tag="ABEKA_RS17090"
                     /old_locus_tag="ABEKA_3583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005253922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_005268593.1"
                     /translation="MLKQSSLERTEFKEEELAPIKKLDLNDLKVPMPKNLDQLLIWTSE
                     LAQYNYAQFLNQSEQLKLVDEDNLEEQAEISQKLRFYRGNALIENQIKDFYLKKLLDQG
                     EATVKGYIKSPTKKNTRYAVISYHDYDFVSFATPEILNKFPADLINFVQKETPKTLADI
                     YPEEAVFLSAIKKFIKPFKEQYKKLADKNREIKEHNTVAHKIYIKIKNKEATLQKPTES
                     KPKESNPKTTENSAVPVEMKQGTIQKNMAPQEKPLTVIKKRKFALNKNK"
                     /name="hypothetical protein"
     gene            complement(2802..3446)
                     /locus_tag="ABEKA_RS17095"
                     /old_locus_tag="ABEKA_3585"
     CDS             complement(2802..3446)
                     /locus_tag="ABEKA_RS17095"
                     /old_locus_tag="ABEKA_3585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005253921.1"
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_process="GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="WP_005253921.1"
                     /translation="MIYFNEFLLLAIIHFLAVVAPGPDFAVVVQQSISYGRKIGAITAL
                     GIGAGISVHVIYTILGFGLLIQTNAIVFNTVKIISALYLIYLGINLIKGNLKKEKSNVN
                     DSEITINNNDLTSKKSAFLKGFITNATNPKATIFFLGIFTTVVNQNTPMQVQFLYGIWM
                     CMVNAGWFILVAYLFTTKNIRNVFLKKKGLFEIFMGIVLIGLALRLLLTFN"
                     /name="LysE family transporter"
     gene            complement(3511..3933)
                     /locus_tag="ABEKA_RS17100"
                     /old_locus_tag="ABEKA_3586"
     CDS             complement(3511..3933)
                     /locus_tag="ABEKA_RS17100"
                     /old_locus_tag="ABEKA_3586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005253919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraD"
                     /protein_id="WP_005253919.1"
                     /translation="MNNDWLSEKITYISALKNPSDAQKLLLELAKIQYRTSDQEKKINA
                     LIKAEKAIDRANKQKVAVRKLLNAEKEAERKARTRHLIQLGALFEIAKLDQRDPAELLG
                     VLIKTAEIDPNDMKWQIWKDLGQEMLNKRSNENKKS"
                     /name="conjugal transfer protein TraD"
     gene            4214..4369
                     /locus_tag="ABEKA_RS17105"
                     /old_locus_tag="ABEKA_3587"
     CDS             4214..4369
                     /locus_tag="ABEKA_RS17105"
                     /old_locus_tag="ABEKA_3587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005098410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004812002.1"
                     /translation="MQLKQVLGKIKDITFGVIAAPLIIIIMMWASILDQRAFKKERNRK
                     KEQDKT"
                     /name="hypothetical protein"
     Typing          4370..4677
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="96.117"
                     /coverage="100.0"
                     /evalue="1.89e-56"
ORIGIN
        1 tcaaaatatc cgtatcaatt tgcgattcat aaccctaaag caatggacgg caaggatcag
       61 cctcacgttc acctcatgtt taatgaacgt ctgcaagatg gaatcgaacg agatccagag
      121 caatatttca aacgctataa cgccaagaat cctgaacgtg gcggagctaa aaaagacaat
      181 acgggcaagg gttaccagga acgaaaaact gatattaaag acctacgcca aagatgggct
      241 gatttatgca acagccattt agaaaaacac caaattgaca gtcgtattga tatgcgaagc
      301 tacaaagagc aaggcataga gaaagaaccc gaaaagaaac tattaccaag ccaagctaaa
      361 gaccctgaaa tcagggaagc cttgcaacag tcacgtatag cctataaaga gcttgagcaa
      421 ctagatttag gcgaccctaa aaaagacctg aaagacctca aaaacagccc aatttcagat
      481 aaagaaatta aacagggtat tgagagcttt aaggctgatt ttgacagctt taaacagctt
      541 gctttggaac agtacaaaca gcagcagaaa ttagaaagag agcaacagaa aacaatgaag
      601 tttagaggga tgagccgatg acacctgatg aaaaaaagct atatgcactg atggcagttg
      661 cagaacaaca gcagaaccta gtcaatcagg ccgttaaaga gcttcaaatc acaaaagaac
      721 acattcaaag cactattgct gggcaaaccc accacactat atcaaaagcg gtaaatgagg
      781 gcttacagga cggtacagca gaacttatta agaccgctaa agcgcttgga cgcttgaaaa
      841 actcgattga atcagccagt gatcaatttt catggaagtt aatcaccact ttcttaggtg
      901 ttttttcagt tttcatactg actatgacat ggttatttgc atcttatatt aagcctttag
      961 agaatattcc aaagatcgag agtttaaagc agcaaggtat tcagtttgat attcaaagtt
     1021 gtgccgttgg agaagagtcc aagccttgcg tccgagtgat gaaagagcag tgtggctatg
     1081 ggaagcatgg ggatttatgc gttattgacc ccaaatagct gaatctgaaa atgaaggatc
     1141 taagcactca tttataattt ttattatgtt taaaattagt ggaagttaag gaaattatta
     1201 tgttaaagca atcaagttta gaaagaacag agtttaagga agaagaactt gcgcccatta
     1261 aaaagcttga tttgaatgat ttgaaagttc ctatgcctaa aaaccttgat cagttactga
     1321 tttggacatc cgaactggct caatataatt atgctcaatt ccttaaccaa tccgaacagc
     1381 taaaacttgt tgatgaagac aacctggaag aacaggcgga aatcagtcag aagcttaggt
     1441 tctatagagg taatgcgctt atagaaaacc aaatcaaaga tttctatttg aaaaagttac
     1501 ttgatcaagg agaggcaaca gtcaaaggtt atattaaaag tccgactaag aaaaataccc
     1561 gatatgctgt aatttcttat catgactatg attttgtaag ctttgctact ccagagattc
     1621 ttaataaatt tccagccgat ctcatcaatt tcgtgcaaaa ggaaacacct aagacattag
     1681 cggacatata tcctgaagaa gccgtgttct tatcagctat caaaaaattt ataaaacctt
     1741 ttaaggaaca gtataaaaaa ttggctgata agaacaggga aattaaggag cacaatacag
     1801 ttgctcataa aatttatata aaaattaaaa ataaggaagc aactctgcaa aagccaacag
     1861 agtcaaagcc taaagaatct aatccaaaga ctactgaaaa tagtgctgtc ccagtagaga
     1921 tgaagcaggg aaccattcag aaaaatatgg ctccacaaga aaagccactt actgtgatta
     1981 agaagcgaaa atttgcttta aacaagaata agtaacttgt aatgtaaaat tctaaacaaa
     2041 aacccctcta agtcttgcaa cttagagggg ttttcaagta aattcagcgg tacgcagtta
     2101 tttgatggaa ctaacatcaa gtaacgaaga tcataaggtt tggcgaccgt tatgatttca
     2161 ccaaaaggct tgtacctgaa ctctattttc acaataaaca gtgtttatat caagcctcaa
     2221 aatgataagt cttcgttaat tcgtgtttta aaccatcaaa taagcatcct agatcgagct
     2281 ttgcccgctt ctgcgtgtcc gttttacggt cggcatgcag tacgtgtggt aggaataagt
     2341 gtcactggcg tattgtagct atagtcgaaa ggctaaaccg cttacagggg aacttcacat
     2401 aggcggagcg ttaggagtag ttacatagga cttgttaagt tgtacgttgg ggaaaccctc
     2461 gcagagatac aggcaatgta acgacaggga gcgcgtggag cgaggtgcaa gactttgagt
     2521 ccttgtctgc atagtcagca aagctatgtg cggatacgac cgatatacgt ggctccgaag
     2581 ccaaatatat acaggtaaat cggcaatact taggggatat gtcatttttt caaatggctg
     2641 cccttagggt gagtattgcc gaaactctct caaccgtttc caaagccaaa gcacgaagtg
     2701 cgacgctttg gaggcgagtt gagaagttga gagcacttgc ttttagcttt tattgtgttt
     2761 agtagtcgag gtagttgttt ttacttttct gcttaggcta ttcagttgaa tgttaagaga
     2821 agtcgtaacg ctaagcctat taacacgata cccataaaaa tttcgaataa tccttttttc
     2881 tttaagaata catttctaat atttttagta gtaaataaat acgctactaa aataaaccat
     2941 ccagcattta ccatgcacat ccaaattcca tataaaaatt gaacttgcat aggggtattt
     3001 tgatttacaa ctgttgtaaa aattcctaag aagaaaattg ttgcttttgg atttgtagca
     3061 tttgttataa atccttttaa gaatgctgat ttttttgaag ttaagtcatt attattaatt
     3121 gttatttctg aatcatttac gttagatttc tcttttttaa gattaccttt aattaagtta
     3181 attcctagat atattagata taaggcagat attattttaa ctgtattaaa tacaatagca
     3241 tttgtttgaa tcagtaatcc aaaccctaaa attgtataaa ttacatggac tgaaattcct
     3301 gctcctatac ctaaggctgt tattgctcct atttttctac cgtaagaaat actttgttga
     3361 acaactacag cgaagtctgg tcccggtgct acaactgcta aaaaatgaat aattgctaaa
     3421 agtaggaact cattaaaata aatcattgaa gtcttttgtc atatgaattg aacgcaatca
     3481 taaattaata tggtaaacat caactaaact ttaagatttt ttattttcat tacttctttt
     3541 atttaacatt tcttgtccta aatctttcca aatttgccac ttcatatcat ttggatcaat
     3601 ttcagcagtt tttataagta ctccaagcaa ttcagcagga tcacgctgat ctagcttagc
     3661 aatctcaaaa agtgcaccta attggataag gtggcgtgta cgagctttac gttcagcttc
     3721 cttttctgca ttcaataatt ttctaacagc aactttttgc ttatttgctc gatctatggc
     3781 tttttcagct ttgatcaagg cattaatttt tttctcttgg tcagatgttc tgtactgaat
     3841 tttggctaat tcaagtaaaa gtttttgtgc atcactaggg ttctttaaag cagaaatata
     3901 ggtgattttt tcagacagcc aatcattatt catattcaat tccattaatt cagataccgc
     3961 aaaagtacag gcttaattct caaagaacga taacaaaata attacgtcaa atgtacgcaa
     4021 ttttgttaaa gtcgttctag agattgttgc ctgcacttcc atcaaaaatt tgatggcgcg
     4081 cttagacgtt tctcaatttc attgagaaac ctaagcacgt tagggacttc atccctaaaa
     4141 ccctgaaact tatttttttc gttcaattga aaattgaatg aaaaaaataa gtaccaaagc
     4201 taggagcaac agcatgcaac taaaacaggt attgggaaaa atcaaagata ttacttttgg
     4261 cgtgatcgca gcaccgctaa tcatcatcat tatgatgtgg gcttcgatac ttgatcaaag
     4321 agcgtttaag aaagagcgca accgaaaaaa ggaacaggat aaaacctaat ggcgattgct
     4381 cgtttaagtg tcaaagtcgg taaggcaggt aaagcagcac cgcatgccga atatattgat
     4441 agggatgaag aaaagaagct gaaacaggaa caggcagaaa ctgatcttga acatagtgct
     4501 tatggaaaca tgccgaaatg ggctgaacat aatccgatta ccttttggca ggcagccgac
     4561 ctttacgagc gtaaaaatgg cagtacctat cgagaatatg aaattgccct gcctagagag
     4621 atgaatgccg agcaacgcct agaactggtt gaggacttca ttcagtctga gatcggc
//
