LOCUS       NZ_CP080641             4760 bp    DNA     circular CON 31-MAR-2024
DEFINITION  Acinetobacter lwoffii strain EK30A plasmid pALWEK1.14, complete
            sequence.
ACCESSION   NZ_CP080641
VERSION     NZ_CP080641.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN05251105
            Assembly: GCF_019787625.1
KEYWORDS    RefSeq.
SOURCE      Acinetobacter lwoffii
  ORGANISM  Acinetobacter lwoffii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Acinetobacter.
REFERENCE   1  (bases 1 to 4760)
  AUTHORS   Mindlin,S., Beletsky,A., Rakitin,A., Mardanov,A. and Petrova,M.
  TITLE     Acinetobacter Plasmids: Diversity and Development of Classification
            Strategies
  JOURNAL   Front Microbiol 11, 588410 (2020)
   PUBMED   33304332
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4760)
  AUTHORS   Rakitin,A.L., Ermakova,A.Y., Beletsky,A.V., Petrova,M.,
            Mardanov,A.V. and Ravin,N.V.
  TITLE     Genome analysis of Acinetobacter lwoffii strains isolated from
            permafrost soils aged from 15 thousand to 1.8 million years revealed
            their close relationships with present-day clinical isolates
  JOURNAL   Unpublished
REFERENCE   3  (bases 1 to 4760)
  AUTHORS   Rakitin,A.L., Ermakova,A.Y., Beletsky,A.V., Petrova,M.,
            Mardanov,A.V. and Ravin,N.V.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2021) Institute of Bioengineering, Research Center
            of Biotechnology of the Russian Academy of Sciences, Moscow 119071,
            Russia
REFERENCE   4  (bases 1 to 4760)
  AUTHORS   Mindlin,S.Z., Beletsky,A.V., Mardanov,A.V. and Petrova,M.A.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2020) Institute of Bioengineering, Research Center
            of Biotechnology of the Russian Academy of Sciences, Moscow 119071,
            Russia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.8-beta
            Genome Coverage                   :: 158.0x
            Sequencing Technology             :: Illumina; Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_019787625.1-RS_2024_03_31
            Annotation Date                   :: 03/31/2024 06:12:30
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,448
            CDSs (total)                      :: 3,336
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 112
            rRNAs                             :: 7, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 222
            CDSs (without protein)            :: 222
            Pseudo Genes (ambiguous residues) :: 0 of 222
            Pseudo Genes (frameshifted)       :: 80 of 222
            Pseudo Genes (incomplete)         :: 138 of 222
            Pseudo Genes (internal stop)      :: 46 of 222
            Pseudo Genes (multiple problems)  :: 39 of 222
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP080641.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4760
                     /organism="Acinetobacter lwoffii"
                     /mol_type="genomic DNA"
                     /strain="EK30A"
                     /isolation_source="permafrost, Kolyma lowland"
                     /db_xref="taxon:28090"
                     /plasmid="pALWEK1.14"
                     /geo_loc_name="Russia"
     gene            complement(join(4674..4760,1..687))
                     /locus_tag="ABEKA_RS17055"
     CDS             complement(join(4674..4760,1..687))
                     /locus_tag="ABEKA_RS17055"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_202034638.1"
                     /translation="MVNGFQWLRFKELFAFLPFLSICLAGYYKYSFYKELNLLWVLSEV
                     SLSQMLFNSIPLFIDALVGFILGLTICIGYARARSKPFLLGFFVAFVLSSFIGYLITPI
                     SSSFLEIIKQGSITIYIAFLFFLFFIYKSTLSDSVRYSISLILVFLLVLFQPLINFKSS
                     KEIEKLLQGHSSYSRVYFKEKSKEAPKNFIEIPRTIQQKPMKLQLDWRIVEVIGDKVVV
                     ICITKVIFDQDMRTRNGSVKRMVKIVEYKDIEKIF"
                     /name="hypothetical protein"
     gene            complement(742..1695)
                     /locus_tag="ABEKA_RS17060"
                     /old_locus_tag="ABEKA_3578"
     CDS             complement(742..1695)
                     /locus_tag="ABEKA_RS17060"
                     /old_locus_tag="ABEKA_3578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005146417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_202034639.1"
                     /translation="MQQLTIGLEQFTNRLPNKPYCSDDLYLGLQVRPKHLALLKRYIQP
                     NHPYYTHFFVFDLDSPSAYVDYFYSMVGVPTPNLIIENPENGHAHFIYQLATPIYNTDA
                     SKPKPIQYANAVYMALRDALEADKSYSGLITKNAVHEHWRTNILREQPYTLDQLSERLD
                     LTAKQINKEIKIDEAVGLGRNCCVFHKVRHWAYVEVRQYRSKSFNQWLDAVIAQCCSVN
                     LQFTVPMQYNEVKGIAKSIARWVWKRDGYAYQEFIDRQKRKGQLGGLKGGVVRSAKYED
                     KRKQAIELSKKGLTQVQIATTLGVSTRTLRNWKSGK"
                     /name="replication initiation protein"
     gene            complement(1910..2368)
                     /locus_tag="ABEKA_RS17065"
                     /old_locus_tag="ABEKA_3579"
     CDS             complement(1910..2368)
                     /locus_tag="ABEKA_RS17065"
                     /old_locus_tag="ABEKA_3579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004884388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid replication DNA-binding protein"
                     /protein_id="WP_228747363.1"
                     /translation="MFNKSEQELATMSKLSINQASKLFKVSRNTIYARIKKGDITKDSE
                     GLVSAQDMMRLFGNKIDKKATEKAVIELLNSREQTVQKSEQDIEQLNGNTEQLLLQQIE
                     QLRLQVEQLEKQLEYVKANEAWLKQQLDQRLIEHKNSEKKGLLSKLFG"
                     /name="plasmid replication DNA-binding protein"
     gene            2536..3114
                     /locus_tag="ABEKA_RS17070"
                     /old_locus_tag="ABEKA_3580"
     CDS             2536..3114
                     /locus_tag="ABEKA_RS17070"
                     /old_locus_tag="ABEKA_3580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018680072.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_202034636.1"
                     /translation="MLIGYARTSTKDQKYSLEAQIEQLTQAGCEKHYEEQVSSVDAKRE
                     QLEIALDFIREGDVLVVTKLDRLARSVADTVDIQKRLEEKKAGLKILDMQIDTTTSTGK
                     FQFNLFAAIAQFEREVMLERQRVGIEKAKTEGKYKGRKPLSTKKKNEIKQLLADNTLTK
                     AAIAEKVKVGIATVYRVKNELSLGSEPLS"
                     /name="recombinase family protein"
     gene            3387..4430
                     /locus_tag="ABEKA_RS17075"
                     /old_locus_tag="ABEKA_3581"
     CDS             3387..4430
                     /locus_tag="ABEKA_RS17075"
                     /old_locus_tag="ABEKA_3581"
                     /inference="COORDINATES: protein motif:HMM:NF023154.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="WP_202034637.1"
                     /translation="MENLPSFSQEEEDLFLASCYALVYEDEQTYKSINSSFDYGQQLIV
                     IEKDLRRLASSNHHSTTNYETLIRLRNSIGEIQRNFPLSSFSPYIDLYFETDKLCRAND
                     KNSNEFLDQLKHALTQTEFKKNIINKRKAVSKNKQSLMRYIEALFEYRARLLVIRVDLG
                     YKRDNSGFINISKSERIDLLDGIKNKEVLEKWSIQVRQHRDDLIKILKKKYKNDLVGYV
                     WKLEYGADKAFHYHTIFFLDGNYHNRDVLIAKEIGELWTTKVTNIQETQGIYWNCNARK
                     NDFRKYDRIATGMIHYKDKRQRENLELMAEYLIKPDYFVRTALPDKARTFGKGERPTKL
                     KSGRPRE"
                     /name="inovirus-type Gp2 protein"
ORIGIN
        1 gatcactttg gtaatacaaa ttactactac cttatcccct ataacttcca caattcgcca
       61 gtctagctgt aacttcattg gcttctgctg aatagttctt ggaatctcta taaaattttt
      121 aggcgcttct ttacttttct ctttaaaata aactctcgaa tatgaagaat ggccctgcaa
      181 gagtttttca atctctttac tacttttaaa attaataaga ggttgaaata agactaaaag
      241 aaatacaaga attaagctaa ttgagtatct gactgaatca gacaaagtac tcttataaat
      301 aaagaataga aagaatagaa aagcgatata aatagttata ctgccttgct tgattatttc
      361 tagaaaagaa gatgagatag gagttattaa gtaaccaata aaggaagata aaacaaaagc
      421 tacaaaaaaa ccaagtaaaa aaggtttaga tctagctcgt gcatagccta tgcaaatagt
      481 taaacccaaa ataaatccta caagagcatc tataaataaa ggaatagaat taaaaagcat
      541 ttggctcaat gagacttcac tcaagaccca taacaaattc aactctttgt aaaatgaata
      601 tttataatac ccagcaagac aaatggataa aaagggtaaa aaagcaaaaa gctctttgaa
      661 gcgtaaccac tgaaatccat taaccataaa tcttcctaaa aagaacaggc aaacccaaaa
      721 aagctaatat ctgatttagg ctcactttcc gcttttccaa tttcttaagg ttcgggtact
      781 cactcctagt gttgtagcta tttgaacttg agttaaacct ttcttgctta attcaattgc
      841 ttgcttacgc ttatcttcat attttgcaga gcgtacaact ccacctttta aaccacctaa
      901 ctgccctttt cttttctgtc tatctataaa ctcctgatag gcatatccat cacgtttcca
      961 tacccaccga gcaattgact tagcgatacc tttcacttcg ttgtactgca taggtacagt
     1021 gaattgtaga ttcactgagc aacattgagc aataacagca tcaagccact gattaaatga
     1081 tttagagcga tattgacgta cttcaacata cgcccaatgt cttactttat ggaatacaca
     1141 gcaatttcga ccaagcccta cagcttcatc tattttgatc tctttattta tctgttttgc
     1201 tgttaaatct aagcgttctg agagctgatc tagcgtataa ggctgttcac gtaggatatt
     1261 agtgcgccaa tgctcatgta cggcgttttt agtaattaaa ccactgtaac tcttatctgc
     1321 ctctaaagcg tctctaagag ccatatatac ggcattagca tactgtatcg gcttaggttt
     1381 acttgcatcg gtgttataaa tcggagtagc tagctgataa ataaaatgag catgtccatt
     1441 ttcaggattt tctataatta aatttggcgt aggaacacca accattgaat aaaaataatc
     1501 aacgtatgcc gatggagaat ccaagtcaaa aacaaagaag tgtgtgtaat agggatgatt
     1561 aggctgaata tagcgtttaa gcaatgctag atgcttggga cgaacttgta gacctaaata
     1621 tagatcatcc gagcaataag gcttgttcgg gagtctgttt gtaaattgtt ctaagcctat
     1681 tgttaattgt tgcattgcat aacttcactg tttgcaaatg ctctaacaac ctgtattatt
     1741 cttctcagat tgttaagcaa atttatgtac tagctgcata agcaagttgt acggtctaag
     1801 gattcaagcc tgtatttcta aactttggcg agcgaggaaa tacgggcttt ttcatgtctg
     1861 gaatatttgt atcataattc catgtagata cgaaatttct aacgcataat tatccaaaca
     1921 acttagaaag taatcccttt ttttcactat ttttgtgttc aataagtctt tgatctaact
     1981 gttgcttaag ccatgcttca ttagctttaa catactccaa ctgcttttct aattgttcaa
     2041 cttgcaatct aagctgttca atctgttgca ataatagctg ttcagtattg ccatttaatt
     2101 gttctatatc ttgctcagat ttttgtactg tttgctctct actgttcaat agctcaatga
     2161 cggctttttc tgtagctttc ttatctattt tatttccgaa taaacgcatc atatcctgag
     2221 ctgatacaag gccttcacta tcttttgtta tgtcaccttt cttgatacga gcataaattg
     2281 tgttacggct aactttaaat aatttacttg cctgattaat tgatagcttg ctcatagttg
     2341 ctaattcttg ttctgattta ttgaacatta atatacaaca aatgacatga tcaaaaataa
     2401 tatcaaaatc ctctatcaat agatcacagt ctattgagag ataaggtttt atgctgctta
     2461 ggctatcaat aggattgttt tttaatttag atagtatatt atctaaatat ctaagaacct
     2521 attgagaaaa attaaatgtt aattggttat gctcgtacaa gtacaaaaga tcaaaaatat
     2581 agcctagaag cccaaataga acagctaaca caggcaggct gtgaaaaaca ctatgaagaa
     2641 caagtatcaa gcgtagacgc gaaaagagag caattagaaa tagctttaga ctttatccgt
     2701 gagggagacg tgcttgtagt aactaagtta gatcgtttag cacgttctgt agcagatacg
     2761 gtagatattc agaaaagatt agaggaaaaa aaggcaggat taaaaatcct agatatgcaa
     2821 atcgacacaa ctacatcaac aggaaaattt caatttaacc tatttgctgc aattgcccaa
     2881 tttgagcgtg aagtgatgtt agagcgtcaa agagtaggaa ttgagaaggc aaagacagaa
     2941 ggaaaatata agggaagaaa acctctttcg acaaaaaaaa agaatgagat taaacaatta
     3001 ttagctgata acactctgac aaaagctgct attgctgaga aagttaaagt aggtattgcg
     3061 acagtgtata gggtaaaaaa tgagctatct ttagggtctg aaccactttc ttaagaataa
     3121 acatttttaa atcaatacaa tcctactaac ttattagctt aaatcaccct aagttatagt
     3181 ctattataga gcgaattccc cctctatcag ggggagccat ttttggctcc ccctgtgtat
     3241 aacggggaat tctgttttag agtttagaag ctgactttag gttcctatgt cattagtact
     3301 aggtagtgca ttagtgctta gtaagggatg tgggtttaac catatgagaa ggctattttg
     3361 tagaaaatta taggtgatag ctttatatgg aaaacctccc aagttttagc caggaagaag
     3421 aagatctatt tctagcgtcc tgttatgcac tagtttatga ggatgaacaa acttacaaat
     3481 ctatcaatag ctcttttgac tacggacagc aattgatagt gattgaaaaa gatttacgcc
     3541 gtttagcctc aagtaatcat cattcaacta caaattatga aacgctaatc cgtttaagaa
     3601 acagtattgg agaaattcag cgtaactttc ccctaagttc attctcacct tacatagact
     3661 tatatttcga gactgacaag ctatgtagag ccaatgacaa aaattcaaat gagtttctag
     3721 atcaactcaa acatgctttg actcaaacag aatttaagaa aaatattatt aataaacgca
     3781 aagctgtcag taaaaataag caaagtttaa tgaggtacat tgaagcactt ttcgaatatc
     3841 gtgcgaggct cttagtcatt cgtgtggatc ttggttataa acgagataat tctggattca
     3901 tcaatatttc aaaaagtgag cgtattgatt tattagatgg aatcaaaaat aaagaagttc
     3961 tagaaaaatg gtcaattcaa gtccgacagc accgtgacga cttaataaaa attttgaaga
     4021 agaaatataa aaatgattta gttggatatg tatggaagct tgaatatggt gcagataagg
     4081 cctttcacta ccacacaata ttcttcctag atggcaatta tcacaatcgt gacgtactta
     4141 tagctaaaga aattggagag ctttggacaa caaaagtaac taatatacaa gagacacaag
     4201 gcatatattg gaactgtaat gctagaaaaa atgattttag aaagtatgac cgtattgcta
     4261 caggaatgat tcactacaaa gataaaagac aaagagaaaa cttagaactt atggctgaat
     4321 acttgatcaa acctgactat tttgtaagaa ctgcacttcc tgataaagct agaacgtttg
     4381 gcaaaggtga aagacctaca aaacttaaat caggaagacc aagagaatag aaaagaattc
     4441 agatatacat catctaagaa aaagtaagtt aatctttgac aaagataaga taagcaataa
     4501 accaacctat ggggcctgaa caaactaaga aaaatagaag ccccaactta aatatatctc
     4561 ctaacatccc ctatcctttt tttgttttta attgaaatac taatattaaa ggaaaagtcc
     4621 ctttaaaaaa gatagtttag ttacctacca agtcaacctt aaagcttttc aaattagaat
     4681 attttttcta tatctttata ttcaacaatt ttaaccattc tttttactga accattcctt
     4741 gttcgcatat cctgatcaaa
//
