LOCUS       NZ_CP080640            11382 bp    DNA     circular CON 31-MAR-2024
DEFINITION  Acinetobacter lwoffii strain EK30A plasmid pALWEK1.12, complete
            sequence.
ACCESSION   NZ_CP080640
VERSION     NZ_CP080640.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN05251105
            Assembly: GCF_019787625.1
KEYWORDS    RefSeq.
SOURCE      Acinetobacter lwoffii
  ORGANISM  Acinetobacter lwoffii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Acinetobacter.
REFERENCE   1  (bases 1 to 11382)
  AUTHORS   Mindlin,S., Beletsky,A., Rakitin,A., Mardanov,A. and Petrova,M.
  TITLE     Acinetobacter Plasmids: Diversity and Development of Classification
            Strategies
  JOURNAL   Front Microbiol 11, 588410 (2020)
   PUBMED   33304332
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11382)
  AUTHORS   Rakitin,A.L., Ermakova,A.Y., Beletsky,A.V., Petrova,M.,
            Mardanov,A.V. and Ravin,N.V.
  TITLE     Genome analysis of Acinetobacter lwoffii strains isolated from
            permafrost soils aged from 15 thousand to 1.8 million years revealed
            their close relationships with present-day clinical isolates
  JOURNAL   Unpublished
REFERENCE   3  (bases 1 to 11382)
  AUTHORS   Rakitin,A.L., Ermakova,A.Y., Beletsky,A.V., Petrova,M.,
            Mardanov,A.V. and Ravin,N.V.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2021) Institute of Bioengineering, Research Center
            of Biotechnology of the Russian Academy of Sciences, Moscow 119071,
            Russia
REFERENCE   4  (bases 1 to 11382)
  AUTHORS   Mindlin,S.Z., Beletsky,A.V., Mardanov,A.V. and Petrova,M.A.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2020) Institute of Bioengineering, Research Center
            of Biotechnology of the Russian Academy of Sciences, Moscow 119071,
            Russia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.8-beta
            Genome Coverage                   :: 158.0x
            Sequencing Technology             :: Illumina; Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_019787625.1-RS_2024_03_31
            Annotation Date                   :: 03/31/2024 06:12:30
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,448
            CDSs (total)                      :: 3,336
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 112
            rRNAs                             :: 7, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 222
            CDSs (without protein)            :: 222
            Pseudo Genes (ambiguous residues) :: 0 of 222
            Pseudo Genes (frameshifted)       :: 80 of 222
            Pseudo Genes (incomplete)         :: 138 of 222
            Pseudo Genes (internal stop)      :: 46 of 222
            Pseudo Genes (multiple problems)  :: 39 of 222
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP080640.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..11382
                     /organism="Acinetobacter lwoffii"
                     /mol_type="genomic DNA"
                     /strain="EK30A"
                     /isolation_source="permafrost, Kolyma lowland"
                     /db_xref="taxon:28090"
                     /plasmid="pALWEK1.12"
                     /geo_loc_name="Russia"
     gene            1..951
                     /gene="repM"
                     /locus_tag="ABEKA_RS16975"
                     /old_locus_tag="ABEKA_3562"
     CDS             1..951
                     /gene="repM"
                     /locus_tag="ABEKA_RS16975"
                     /old_locus_tag="ABEKA_3562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004786597.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein RepM"
                     /protein_id="WP_202034713.1"
                     /translation="MRELVVKDNALINASYNLDLVEQRLILLAIVEARESGKGINANDP
                     LEVHAEGYINQFGVHRNTAYQALKDACNDLFARQFSYQKINERGNIENYRSRWVSEIGY
                     VDNEAVVKLIFAPAIVPLITRLEEHFTKYELQQVSNLSSAYAVRLYELLIAWRSTGSTP
                     VIELSDFRQRIGVLDTEYKRMERFKTSVLELAIKQINEHTDITVKYEQHKKGRSISGFS
                     FTFKQKKKDNPSIERDPDTLDLFSKMTDAQRHMFANKLSELPEMGRYSQGTESYPQFAV
                     RIAEMLQDSEKIKELAPYLKKVGYMPSNKKDTVNG"
                     /name="repM"
     gene            944..1519
                     /locus_tag="ABEKA_RS16980"
                     /old_locus_tag="ABEKA_3563"
     CDS             944..1519
                     /locus_tag="ABEKA_RS16980"
                     /old_locus_tag="ABEKA_3563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005102333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid replication DNA-binding protein"
                     /protein_id="WP_202034714.1"
                     /translation="MAKLSLSEVSKKFHIDRSTIYRAVRNGRLSRSSDGQFDLSEVIRC
                     FGEPEQTSQQIESSTSDSDESTKKLIFHLENEVKKYQEREERLMQQIDRMQTLIELKTV
                     APATAAPQQDATACDTDMPQHATTQQDNDNKKNNELNIVENVAVPQQETTAHHSQTLQH
                     ATLQNVAVPQPKKRSLFGRVLSAVFNED"
                     /name="plasmid replication DNA-binding protein"
     gene            1541..1684
                     /locus_tag="ABEKA_RS16985"
                     /old_locus_tag="ABEKA_3564"
     CDS             1541..1684
                     /locus_tag="ABEKA_RS16985"
                     /old_locus_tag="ABEKA_3564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004676226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_202034715.1"
                     /translation="MKKLKEILQGIAFLFIGIPIMIIASIMDQRAFQKELKERQKEKQL
                     QE"
                     /name="hypothetical protein"
     gene            complement(1874..>2074)
                     /locus_tag="ABEKA_RS16990"
                     /pseudo=""
     CDS             complement(1874..>2074)
                     /locus_tag="ABEKA_RS16990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004986464.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /name="IS30 family transposase"
     gene            complement(2168..3196)
                     /locus_tag="ABEKA_RS16995"
                     /old_locus_tag="ABEKA_3566"
     CDS             complement(2168..3196)
                     /locus_tag="ABEKA_RS16995"
                     /old_locus_tag="ABEKA_3566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="WP_079255106.1"
                     /translation="MIRRIKHASTATCTLPIYMGFLMTEPNSISCTQLAETYNISHDSV
                     NRFLEREDYTPHDLYQESIQHIDNNKLIVSIDDTVLDKPYSQHMDLVSYFWSGKHHRSV
                     KGINLITLYATDQNGQNIPINFRIYDKSESKTKNDYFMDMLSEVLSWGAKIQFITGDSW
                     YSSTGNLKTIRKYGIRFMFGIDCNRKVSPEKGQWFQLRLLPDFHQGQVVWLKDFGFVQL
                     FKTQLKEQQRFYIVHQDEDDLLSFEGFHELHSSHWKIEQYHRVIKQVCHIEKFQVRRSK
                     LILNHIFSALMAYVEIQKNQFERIFENVYRWQKKLFRPVIKNFIDDFILDKNHLLPQRI
                     YK"
                     /name="transposase"
     gene            3260..3769
                     /locus_tag="ABEKA_RS17000"
                     /old_locus_tag="ABEKA_3567"
     CDS             3260..3769
                     /locus_tag="ABEKA_RS17000"
                     /old_locus_tag="ABEKA_3567"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_202034716.1"
                     /translation="MNKAFIFVMTGFVLSGCIATGNKIIDLKARPEITVLENGYKQIST
                     TPFSITQEGGGIDSVHAAVGASWRNDRNDYLTINVRSFHKSSLEWMRLIIDEKVYQYSP
                     ISPCVNSKFTCYEIPVDVAKEMLNAVSVRIEYKTESGSIHKGFLKHGDIILPGGKTVLN
                     VIRAIP"
                     /name="hypothetical protein"
     gene            complement(3816..>4748)
                     /locus_tag="ABEKA_RS17005"
                     /old_locus_tag="ABEKA_3568"
                     /pseudo=""
     CDS             complement(3816..>4748)
                     /locus_tag="ABEKA_RS17005"
                     /old_locus_tag="ABEKA_3568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004729329.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /name="IS4 family transposase"
     gene            complement(4804..5568)
                     /locus_tag="ABEKA_RS17010"
                     /old_locus_tag="ABEKA_3569"
     CDS             complement(4804..5568)
                     /locus_tag="ABEKA_RS17010"
                     /old_locus_tag="ABEKA_3569"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_202034717.1"
                     /translation="MNTIYFLKNYSSLSERFDEGPFRFERVYLDLYSRDEDDDTEDENI
                     EFDDETHCCFSLNMANKIYEFVSINTNKSYLVCERCLVLDFISNEENIKKVKKIKNDQD
                     FLEENNLKIGIVVQLKSKEGPLMTVESVIDHKILCCWFVGTKLFRGEFSPLVLVSIKKN
                     SPLEINNLEEIAKNMKNKLIKDRYLEQESAAIWLEQIYGEAAVYINQYGNLAINKSVLK
                     AFEEITSTNVVWDKVNKLWRLRESGDSNPRGQ"
                     /name="hypothetical protein"
     gene            5611..5907
                     /locus_tag="ABEKA_RS17015"
                     /old_locus_tag="ABEKA_3570"
     CDS             5611..5907
                     /locus_tag="ABEKA_RS17015"
                     /old_locus_tag="ABEKA_3570"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_202034718.1"
                     /translation="MLIKQGSIVQLNSGSPNLVVESISIYMTEGNGKYYSISQLPAHIN
                     NTELKKYLAEKIDIKIDDLPIEAIQISLDCIWFQGKEPVSDSFDFRLLNLISS"
                     /name="hypothetical protein"
     gene            complement(6373..6696)
                     /locus_tag="ABEKA_RS17020"
                     /old_locus_tag="ABEKA_3571"
     CDS             complement(6373..6696)
                     /locus_tag="ABEKA_RS17020"
                     /old_locus_tag="ABEKA_3571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000681328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000681328.1"
                     /translation="MKHLGFVGKSTEYIGYTHVEIINSSLRLSFNVPLTNNVEETFKKI
                     VDNNYDLLMSTSAEMSFELENETLEILEKVIREQGIETEEFIQNTLIKFINQNGLDTTK
                     SNL"
                     /name="hypothetical protein"
     gene            complement(6750..7064)
                     /locus_tag="ABEKA_RS17025"
                     /old_locus_tag="ABEKA_3572"
     CDS             complement(6750..7064)
                     /locus_tag="ABEKA_RS17025"
                     /old_locus_tag="ABEKA_3572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004999435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_000197509.1"
                     /translation="MTRLMTEKELQAFEATRDIEAEILQGIDDMLANNAAKRTVITETD
                     VALARRKAQLTQDQFAKILGVSRRTLESWEQGARRPSGAATSLIRLFIVDPKFVKESLA
                     "
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(7045..7368)
                     /locus_tag="ABEKA_RS17030"
                     /old_locus_tag="ABEKA_3573"
     CDS             complement(7045..7368)
                     /locus_tag="ABEKA_RS17030"
                     /old_locus_tag="ABEKA_3573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000288001.1"
                     /translation="MYTICETPLFTKYCLVYWTQEEYEEFKTFLALNPEAGDVEPNSGG
                     IRKIRWTSGGRGKSGGVRVIYFNRLTNGEIWLLTLYSKKQTVQLSKKTLQALVEKLNDS
                     FND"
                     /name="hypothetical protein"
     gene            7512..7817
                     /locus_tag="ABEKA_RS17035"
                     /old_locus_tag="ABEKA_3574"
     CDS             7512..7817
                     /locus_tag="ABEKA_RS17035"
                     /old_locus_tag="ABEKA_3574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010326913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000734383.1"
                     /translation="MKKLKSQYQQSVKTYPHDYFSKRYPNIRSVYFKKFSTQQERSFYF
                     MHHIEYKNYPLKFRVARGTGLPHVWDDFPTYVYKIAKSWKHNSKRAHQYYKEHEQK"
                     /name="hypothetical protein"
     gene            7944..8597
                     /locus_tag="ABEKA_RS17040"
                     /old_locus_tag="ABEKA_3575"
     CDS             7944..8597
                     /locus_tag="ABEKA_RS17040"
                     /old_locus_tag="ABEKA_3575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000282938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000282938.1"
                     /translation="MYNVHPSEKLIQAFRQKPYQGCCPTQAEFLFIGLDANYAPNVEEQ
                     KIFSKIIEYHEDAISFWQKYNLHHPFLLDGYKGDGRKYHKEFSKIRLSCKDASRISFIE
                     LLHLPTTGRNDLKSSDLDKNHLQYIHKAIFSEHTKAVFISDAVFKLMKKTSIFSWMNDA
                     KQIEGHTLKVFHEKKPLIYKHLHFSTYGKFQAQKEEEIKAIHNIILGSNISDLT"
                     /name="hypothetical protein"
     gene            complement(8631..8891)
                     /locus_tag="ABEKA_RS17045"
                     /old_locus_tag="ABEKA_3576"
     CDS             complement(8631..8891)
                     /locus_tag="ABEKA_RS17045"
                     /old_locus_tag="ABEKA_3576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005000600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_031974998.1"
                     /translation="MSVETLEQKIAKQEEVLKQLKAQKQAVIAREKKKQSEQKRKDETR
                     RKILLGSLMLKKMEDEANKEKILADLNEYLTEDRDRKLFNL"
                     /name="hypothetical protein"
     gene            9103..10758
                     /gene="mobQ"
                     /locus_tag="ABEKA_RS17050"
                     /old_locus_tag="ABEKA_3577"
     CDS             9103..10758
                     /gene="mobQ"
                     /locus_tag="ABEKA_RS17050"
                     /old_locus_tag="ABEKA_3577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005199240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobQ family relaxase"
                     /protein_id="WP_202034712.1"
                     /translation="MAIYHFSVKTISRGNGRSAVACAAYRSGEKLVCDFYGKEQDYTKK
                     TGVEFTEIYAPKNTNTELLNRQTLWNQVEKAERRKDALLAREFEIAFPSELNAEQRKNM
                     LNELCQDLVKKYGVIVDAAIHAPHTGNGSDERNYHAHIMFTTRSINEHGDFSAKKYRDF
                     SRDNGTETVSHWRELFAELCNHHLEKNGFDERVDHRSYEDQESDLEATQHEGPQATYLR
                     RRGIFTEISLKNDEIKLRNLEIKKGIALDENIKVSEDLVQQVRSELQFQYSKAEYLEKI
                     SQKLSEFQDEELSKLTTELNSMNSEISELHKKEPLFFKTKWSNQINDLIDQHNIQLDIK
                     KHISSMSEIEKKDRYSNQLNKWTEENQLLQPKKSFEKFDETSITVKQRKLNDEILNIDH
                     QLAEISRREGEYQTYTRTQLIELRNSYIFEEDDYGNKYFSPQNGEKQKRLYAKEMEELK
                     IQKMHADFIKPIEVEAEQEFAKKRAIQLENDRKDRESREQQLRKEREQQEQRYEQERRE
                     QDHMAFLRRQELEKQQKNEPKKPEQDNDNDYTPW"
                     /name="mobQ"
     Typing          2..951
                     /name="rep_cluster_1172"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="92.219"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          9104..10755
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="94.737"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 atgagagaat tagtggtaaa agacaatgcc ttaatcaatg caagctataa cttggattta
       61 gtagaacaac gtttaatttt attggctatt gttgaagcaa gggaaagtgg gaaagggatt
      121 aatgctaatg atccccttga agtacatgcc gaaggctata tcaatcaatt tggtgtacat
      181 cgcaatactg cctatcaagc attaaaagat gcctgtaatg atttatttgc tagacaattt
      241 agttatcaaa aaataaatga aagagggaat attgagaact atagatcccg ttgggttagt
      301 gaaattggat atgtagataa tgaagcagtg gttaagctta tctttgcccc cgccatagtc
      361 ccattaatta cacgtttaga agagcatttt actaaatacg aattacagca agttagtaat
      421 ctcagtagtg cttatgctgt ccgcttatat gaactattaa ttgcttggag aagtaccggt
      481 tctactccag ttatagagct aagtgatttc cgtcaaagaa ttggtgtact cgatacagag
      541 tacaagcgta tggagcgctt taaaactagt gtacttgagc ttgctattaa acaaataaat
      601 gagcatacgg atataactgt aaaatatgaa cagcataaaa aaggtcgatc aatttcaggt
      661 ttttctttta cgtttaaaca gaagaaaaag gataatccat caatagaaag agatccggat
      721 accttagacc ttttttcaaa gatgaccgat gctcaacggc atatgtttgc aaacaaactt
      781 tcagaactcc ctgaaatggg tcgctattca caaggaactg aaagctatcc tcaatttgct
      841 gttcgtattg ctgagatgct acaagactct gaaaaaatca aagaactagc cccataccta
      901 aaaaaagtgg gatacatgcc atcaaataaa aaggacacag taaatggcta agttatcgct
      961 aagtgaagta tctaaaaaat ttcatattga taggtcaacc atttacagag ctgtacgtaa
     1021 tgggcgttta tcacgctcta gtgatggtca atttgacctt tcggaagtga ttagatgctt
     1081 tggggagcct gagcaaacat ctcaacaaat tgaatcttct acatcagata gcgatgaatc
     1141 tacaaaaaaa ctcatttttc atttagaaaa tgaagttaaa aaataccaag aacgtgaaga
     1201 gcgtttaatg cagcaaatcg accgtatgca gacgcttatt gagttaaaaa ctgttgcacc
     1261 cgccacggca gcgccacaac aagatgctac ggcatgcgac accgatatgc cacagcatgc
     1321 gacaacacaa caagacaatg ataataaaaa gaataatgaa ttaaatattg tagaaaacgt
     1381 ggcagtgcca cagcaagaaa ctacagcaca ccacagccaa acgctacagc atgccacatt
     1441 gcaaaatgtg gcagtgccac aacctaaaaa acgtagctta tttggccgag tactgagtgc
     1501 ggtattcaat gaggactaag ctctccaagg agcattacgt atgaaaaaac tgaaagagat
     1561 cctacaaggc attgcatttc tatttattgg tattcccatc atgatcattg ccagtattat
     1621 ggatcaacga gcctttcaga aagaactaaa agaacgccaa aaagaaaagc aattacagga
     1681 atgactattt atctgatcta atcaaaaccg tggaacattg ctcaagaatt aagcgtggaa
     1741 cgtgaaaaat cataaaaaaa gcccaccttg gggaagatgg gctataaaca ggaaactata
     1801 gcattgattt gtatagataa ttataacgca tttcgtataa ggtgtattat gttaatttta
     1861 gggaaaggcg gattcaagcg gcaagtgcaa cagtataaaa accagccata acttcactcg
     1921 gtgtatacca acctagtgtt tttctaggac gatggttgag tgcaaattct atctgctcta
     1981 tttgttcgtt tgacacgcca tcaaacgatg atgattttgg cagatattga cggattaaac
     2041 cattcgtatt ttcatttcta gctcgctgaa tagactagta aaggctcatc gaaaggtggg
     2101 cttttttatt aggggagtga tagactagac atttaaactt tatacaaaaa ataggactta
     2161 cgcactatta tttatagatt ctctgtggta gcagatgatt tttatcgaga ataaagtcat
     2221 caatgaagtt tttgataact ggtctaaata atttcttctg ccaacgatat acattttcaa
     2281 agatccgctc aaactggttc ttttgtatct caacgtaggc catcaaggct gaaaaaatat
     2341 gattcaaaat cagtttagat cgtcttactt gaaacttttc aatatgacaa acctgtttaa
     2401 tcacccggtg atattgctct attttccaat gacttgaatg taattcatga aaaccctcaa
     2461 aggacaataa atcatcttca tcttgatgca caatataaaa cctctgctgt tcttttaact
     2521 gagtcttaaa taattgtaca aagccaaaat ctttgagcca gaccacttga ccctgatgga
     2581 aatctggcaa taaacgcagt tgaaaccatt gtcctttttc tggggaaacc ttacggttac
     2641 agtcgatacc aaacataaat cgaataccat attttcttat ggtttttaga tttccagtcg
     2701 atgaatacca actatcacct gtaataaatt gaatctttgc accccaactg agtacttcac
     2761 ttaacatatc cataaagtaa tcattcttgg ttttactttc agatttgtca taaattcgga
     2821 aattaattgg aatattttga ccattttgat ctgtcgcata caaggtaatg agattaatac
     2881 ccttgacgga tcggtggtgt ttgcctgacc aaaaatagct aaccaagtcc atatgttgac
     2941 tatatggttt atctaaaaca gtatcatcaa tactgactat aagtttatta ttatcaatat
     3001 gttgaattga ttcttgatat aggtcgtgag gtgtgtagtc ttcacgctct agaaagcgat
     3061 ttacactatc atgcgagata ttataagtct cggcaagttg tgtgcagcta atagagttcg
     3121 gttctgtcat gagaaagccc atataaatgg gtaatgtaca agttgctgta gaagcatgtt
     3181 taattcgtct aatcacagat actttataaa gtattttaat acttttttga atccgtcaat
     3241 gcgtaagtcc taaaaaaata tgaataaggc atttatattt gtgatgactg gctttgtgct
     3301 ttcaggttgt atagctacgg gcaataagat aatagatctt aaagcacggc ctgagatcac
     3361 agtactagag aacggttaca aacaaataag cactacaccc ttttctatta ctcaggaagg
     3421 tggtggtatt gactctgttc atgctgcggt aggtgctagc tggagaaatg atagaaatga
     3481 ctatctaact attaatgtgc gatcatttca taagtcaagt ttggagtgga tgaggttaat
     3541 cattgatgaa aaagtatatc aatactcacc aatctcccca tgtgtgaatt ctaaatttac
     3601 ttgttatgaa attcctgtag atgttgccaa agaaatgctt aatgctgttt cagttagaat
     3661 tgaatataaa accgaatctg gaagcataca taaaggattt cttaaacatg gtgatattat
     3721 tcttcctggt ggtaaaactg ttttgaatgt tattagggca attccataat tctttaatca
     3781 attaaaagct cgccaaatgg tgtaatgcca gtcagttaag aaattgactg gcattttctt
     3841 gggtatttct gcggttttcc tttcacaact cgtgggcaac ttctaaccct tctctcaggc
     3901 aaaacatacc tcctcgattt ctccagtaaa ctttctagat gtttaggtaa attccctgcg
     3961 gagtctaaag aatcaaatct taatatattc aggatgctga tagaggcaat atgaaagctg
     4021 attctcaaag gactgacttt tgcacgttga gccatatgtt tcatttgtct tcttagaata
     4081 ttataggcaa tgaagacccc ccataattct tgataaatta aatcaggttg cttgctcctc
     4141 aaatgcttac cttcctgtaa atcacttttg atttctcggt aacacatttc tatttcccag
     4201 cgctgagcat aaagctttgc taaggctagc agtggatata tctttgaatc tattaatgaa
     4261 gtgatatagc gtctaatttt tcctgcctgc tcaacttcaa tcaaacgcgc ttcccaataa
     4321 tcccctaatg ttgaattcag cttcttggct cttgctgata ttggcatcct gatatgaaag
     4381 tcatgctggg aattacgctt aatgatctca tagcgtaaat tatcttttgc gcgcataagc
     4441 caatggcttg cctctgcacg tgtttgccat cctattagaa aatcagcaga aaaataggct
     4501 cgatcaaaca gagtaatact atgcgatgga ggacataatc gatttgccag tgtaagttca
     4561 ccttgatcca tgctacctat ttgggcatca atgatttcat gggtgctggt attcaccaga
     4621 caggttgctc tcacttgcgg ataaggtgct accgcagttt tacccttaga tgagccagag
     4681 tgtgcaaaat tctcatctgt ataaggcata gaccaaacca ccccgtcaac agcacataca
     4741 ctaaggcctt aagaaatcaa tgagtccgct tcccataata tgtattatgt tactttaatc
     4801 tctttactgt cctcttggat tagaatcacc actttctctt aatcgccata acttattcac
     4861 tttatcccaa actacatttg ttgaagttat ttcttcaaat gcctttaaaa cacttttgtt
     4921 tatagccaaa ttaccgtact gattaatata taccgcagct tcaccgtata tttgctctaa
     4981 ccaaatagca gcactttctt gttctaaata tctatcttta attagcttat ttttcatatt
     5041 cttagctatt tcctcaaggt tatttatctc taaagggctg ttttttttta tgctaaccaa
     5101 gactaaagga ctaaactccc ctctaaataa ttttgttcct acaaaccaac aacatagaat
     5161 cttgtggtct atgactgatt ccacagtcat aagaggacct tcttttgatt ttaactgtac
     5221 aactatgcct atttttaaat tattttcttc taagaagtcc tgatcattct taattttttt
     5281 tacttttttt atgttttctt cattactaat aaaatctaaa actaaacacc tttcacaaac
     5341 taaataactt ttatttgtat taattgagac aaactcatat atcttattag ccatattcag
     5401 actgaaacaa caatgagttt catcatcgaa ttctatattt tcatcttcag tatcatcatc
     5461 ttcatctcga ctataaagat caagatatac tctttcgaag cgaaatggtc cttcatcaaa
     5521 tcgttctgaa agagatgaat aattttttaa aaaataaatt gtattcatgt atctgtttac
     5581 tatccaatca ataagtaggt gggctaaaaa atgttaatta aacaaggttc tatagtacaa
     5641 cttaattcag gtagtcctaa tttagtagta gaatcgattt ctatttatat gacagaagga
     5701 aatggtaaat attattcaat tagtcaacta cctgctcata ttaataatac tgaactaaaa
     5761 aaatatttag cagaaaaaat agatattaaa attgatgatt tacctatcga ggctatccaa
     5821 atttccttag attgtatttg gtttcaaggt aaagaaccag tttcggattc ttttgatttt
     5881 cgactgttaa atctaatttc ttcttaaaat tcttttttat ttaacataat gggcgttata
     5941 cgaaacgcct ttttaaatag tattatttat caaatagtta actcaataaa tccaaaaccc
     6001 aaaagatcaa aaaacagacg ttttaacaac tcgtgtgttt tttaagcaga tttgtaatct
     6061 ttcgccaata aaatcgtcta aaaaatgatc aattttggca ttttcccaat gttctcagct
     6121 cttccagggc gaaaactatc cccaattact gtggatgaaa tttaggctat tttgtaagcc
     6181 ctggctcata ccaatctcgg ccctttgctg ctatccagga taggtgaatt ttcctatgat
     6241 gaactcatcg ggaacaggaa gttccataca agaataacaa gagataagca caaggactgt
     6301 aaggtacgac aggagttata ccgagcgaac caatacgaaa aaccccggca ggatgccggg
     6361 gttttttatt gtctaaagat tagatttagt agtatctaat ccattttgat tgatgaattt
     6421 aatcaacgta ttctgtataa attcttccgt ctcgatcccc tgttctctaa ttactttctc
     6481 aagaatttca agcgtttcat tttctagttc aaatgacatt tctgcggatg tcgacatcaa
     6541 taaatcatag ttgttgtcta caattttttt aaaggtttct tcaacattat ttgttaatgg
     6601 cacgttgaaa gacaggcgta gagaactatt gataatttct acatgggtat agccgatata
     6661 ttctgtactt tttcctacaa atcctaagtg tttcataaaa aaccttaaat aatatatagt
     6721 gaatagctct ctagtgagag ctagatgaat taagccagac tttcttttac aaattttgga
     6781 tcaacaataa ataatcggat aagcgatgtt gctgctcctg atggtcttct cgcaccttgt
     6841 tcccatgatt caagagtacg tcttgatacg ccaagaatct tggcgaactg atcttgagtt
     6901 agttgagcct ttctacgagc taatgccaca tctgtttcag tgatcactgt tcttttcgct
     6961 gcattattag ccaacatatc atcaatgcct tgtaatatct cagcttcaat atctctagtt
     7021 gcttcaaaag cttgtaattc tttttcagtc attaaacgag tcatttaatt tctccaccaa
     7081 tgcttgcaga gttttcttac taagctgtac agtttgcttt ttactgtaca aggttaataa
     7141 ccaaatctct ccatttgtta aacgattaaa gtagatgact ctgacaccac cacttttgcc
     7201 cctaccgcca ctagtccatc taatcttacg aataccgcct gaattgggtt ctacatcccc
     7261 agcttcagga tttaatgcta agaatgtttt aaattcctca tattcttcct gagtccaata
     7321 aacaagacag tatttagtaa ataaaggggt ttcacatatt gtatacattg cttgtgttta
     7381 tactaccttg tggtagttaa ttttatacta ctttgtggta cttttaaata ttaattttta
     7441 attcatagga ctaaagacaa tgtactactt tgcagaggat cccttctagg taatgtcacc
     7501 taggaggaaa aatgaaaaaa ctgaaatctc agtatcaaca atctgtaaaa acataccctc
     7561 atgattattt ttccaaacgc tacccgaaca taaggagtgt ctattttaaa aaatttagca
     7621 ctcaacaaga gagaagcttt tatttcatgc atcatattga atataaaaac tatcctctta
     7681 aatttcgtgt agcgcgtggt actggtctac ctcatgtttg ggacgatttt ccaacttatg
     7741 tatacaaaat tgctaaaagt tggaagcata attctaagag agcacatcag tactacaaag
     7801 aacatgagca aaaataaaaa atgggagcta taggctccca tttttgtgtt agaacccatt
     7861 taaaatgtct atattctcac caataaaaat gtccactttt agaataaact aatattcttt
     7921 gcattacgtc tactgataaa aatatgtata acgttcatcc aagtgaaaaa ctaattcaag
     7981 ctttccgcca aaaaccttat cagggatgtt gtcctactca agcagagttt ttatttattg
     8041 gcttagatgc caattacgct cccaatgttg aagaacaaaa aatattttct aaaatcattg
     8101 aatatcatga ggatgctatt tcattttggc aaaaatacaa tttacatcat ccttttttat
     8161 tagatggata taaaggagat gggcgaaaat atcataaaga attttcaaaa attagacttt
     8221 cttgcaaaga tgcttctcgt atttcattta tcgagcttct tcatcttcca acaactggta
     8281 gaaatgattt gaaaagctct gatcttgata aaaatcatct acaatacatt cataaagcca
     8341 ttttttcaga acacactaaa gctgtattta tctctgatgc tgtttttaaa ttaatgaaaa
     8401 aaacatctat attttcatgg atgaatgatg caaaacaaat agaaggacat actcttaaag
     8461 tttttcatga aaaaaaacct ttaatctata agcatttaca tttttcaact tatggaaagt
     8521 ttcaagctca aaaagaggaa gagataaaag caattcataa tattattcta ggatcaaaca
     8581 tcagtgattt aacataaaaa ctcttatacg aaatttgcat gctagaaaca ttaaagatta
     8641 aataattttc tatctctatc ttctgttaaa tactcgttta aatcagcgag tattttttct
     8701 ttattggctt catcttccat tttttttaac atcaaagaac cgagtaaaat tttacgtcta
     8761 gtttcatctt ttcgtttctg ttcagattgt ttcttttttt cacgggcgat aacagcttgc
     8821 ttctgtgctt ttagctgttt taaaacctct tcctgctttg caattttttg ttctaaagtt
     8881 tcaacactca taccagttac cgattcagga ttcttttcga tcatatgcgc atttgaactc
     8941 acgttcaaga tgctatacga tactcaaaga atccccgaag ggcgcactta gccattgagt
     9001 aaaactcaat ggtgcgacaa ggggtaaatc ccctttgtaa tcccctgacc aacatattca
     9061 tgttggtaat ttcgataaat cgaaataaag agcaaaagca aaatggcgat atatcatttt
     9121 tcagttaaaa cgattagtag aggtaatgga cgatcagccg ttgcttgtgc tgcatatcgt
     9181 tcaggtgaaa aattggtatg cgatttctat ggaaaagaac aagactacac aaaaaaaact
     9241 ggtgtcgaat ttacagaaat ctatgcaccc aaaaatacaa atactgagtt actcaaccgt
     9301 cagacacttt ggaaccaagt agaaaaagcc gaacgaagaa aagatgcatt acttgcaaga
     9361 gaatttgaga ttgcatttcc aagtgagtta aacgctgaac agcgtaaaaa catgcttaat
     9421 gaactttgcc aagacctcgt taaaaaatac ggtgtcatcg ttgacgctgc tattcatgca
     9481 cctcatacag gtaacggaag tgatgaacgc aattaccatg cacacatcat gttcaccacc
     9541 cgaagtatta atgagcatgg tgacttttca gcaaaaaaat atagagactt cagtcgtgac
     9601 aatggcacag aaacggtaag ccactggcga gaattatttg cagaattatg caatcaccac
     9661 ctggaaaaaa atggatttga tgaacgtgtc gatcacagaa gttatgaaga ccaggaaagt
     9721 gacctagaag ccactcagca cgaaggtcct caagcgacat acctgcgaag acgagggata
     9781 tttacagaaa ttagtctgaa aaatgatgaa attaagctac gtaatttaga aatcaaaaag
     9841 ggaatcgcac ttgatgaaaa catcaaagta tctgaggatc ttgttcaaca agtacgaagt
     9901 gaacttcaat tccaatactc taaagctgag tatttagaaa aaatttctca gaaactttct
     9961 gaattccaag acgaagaatt atcaaaatta acaacagaat taaactcgat gaattctgaa
    10021 atatcagaac tacacaaaaa agagccttta ttttttaaaa caaaatggag caatcaaatc
    10081 aatgacctaa tcgaccaaca caatattcaa ctagatatca aaaagcatat ctctagcatg
    10141 agtgagatag agaaaaaaga tagatactct aatcagttaa ataaatggac agaagaaaat
    10201 cagctgctcc aacctaaaaa gtcttttgaa aaatttgacg aaacaagcat tacagttaaa
    10261 caacgcaaac taaatgatga aattttaaat atagatcatc aacttgcaga aatatcacgt
    10321 agagaaggtg aatatcaaac atatactcga actcaactca tagagctcag aaatagttat
    10381 atatttgagg aagatgatta tggaaataaa tattttagtc ctcaaaatgg tgaaaaacaa
    10441 aagcgactat acgcaaaaga aatggaagag ctaaaaatcc aaaaaatgca tgcagatttt
    10501 ataaaaccaa ttgaggtaga agcagagcaa gaatttgcca aaaaaagagc aatacagctc
    10561 gaaaatgacc gaaaagatcg tgaatcgagg gaacagcagt tgcgtaaaga acgagaacaa
    10621 caagaacaac gctatgagca agaaagacga gaacaagacc atatggcatt tttaagacgc
    10681 caagaattgg aaaaacagca gaaaaatgag cctaaaaagc cagaacaaga caatgataac
    10741 gattacacac cttggtaaac cctgtttaaa cgcaaaaaac ggggttttta gcgatgtttt
    10801 ggtggagtat atggtttcaa tagccaaatt taaaaagtct cttaaatcga aaatgagagc
    10861 atttagcgag tgtctacgag cgacacaaag aaaattcgcc tatccccccc tctgaaaaac
    10921 agctttttag gctgtgaagt ctaaataagc gtaaatcaca tgcagaaaat tgagcctaga
    10981 ccgagcgtag cgagtaaaaa agctatatga gcgaagcgaa ttccgagttg cttttgctgt
    11041 tgcttttact ttttctaaat tcaggggaac attgaccaaa aaattgcccc gacgaatcaa
    11101 gcgaaagcga gattcaatag agtttgagcg tagcgaaaac caagggcaat ttttcactcc
    11161 ttgggctttt aattatttta aagcttttaa atgcttttag acatgctgaa agcctttgtt
    11221 cacaatgctt tcaagggtta tatgacaccg tttaccttgc aatatgacac cgtttacctt
    11281 gcaatataac accgtttacc ttgcaatatg acaccgttta ccttgcttat agcactaaaa
    11341 ttaattattg tgtcatagca taataattaa tactggtgct tc
//
