LOCUS       NZ_CP080639             8227 bp    DNA     circular CON 31-MAR-2024
DEFINITION  Acinetobacter lwoffii strain EK30A plasmid pALWEK1.5, complete
            sequence.
ACCESSION   NZ_CP080639
VERSION     NZ_CP080639.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN05251105
            Assembly: GCF_019787625.1
KEYWORDS    RefSeq.
SOURCE      Acinetobacter lwoffii
  ORGANISM  Acinetobacter lwoffii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Acinetobacter.
REFERENCE   1  (bases 1 to 8227)
  AUTHORS   Mindlin,S., Petrenko,A., Kurakov,A., Beletsky,A., Mardanov,A. and
            Petrova,M.
  TITLE     Resistance of Permafrost and Modern Acinetobacter lwoffii Strains to
            Heavy Metals and Arsenic Revealed by Genome Analysis
  JOURNAL   Biomed Res Int 2016, 3970831 (2016)
   PUBMED   27795957
REFERENCE   2  (bases 1 to 8227)
  AUTHORS   Rakitin,A.L., Ermakova,A.Y., Beletsky,A.V., Petrova,M.,
            Mardanov,A.V. and Ravin,N.V.
  TITLE     Genome analysis of Acinetobacter lwoffii strains isolated from
            permafrost soils aged from 15 thousand to 1.8 million years revealed
            their close relationships with present-day clinical isolates
  JOURNAL   Unpublished
REFERENCE   3  (bases 1 to 8227)
  AUTHORS   Rakitin,A.L., Ermakova,A.Y., Beletsky,A.V., Petrova,M.,
            Mardanov,A.V. and Ravin,N.V.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2021) Institute of Bioengineering, Research Center
            of Biotechnology of the Russian Academy of Sciences, Moscow 119071,
            Russia
REFERENCE   4  (bases 1 to 8227)
  AUTHORS   Beletsky,A., Kurakov,A., Petrova,M. and Mardanov,A.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JUN-2016) Lab of Molecular Cloning, Institute of
            Bioengineering, Research Center of Biotechnology of RAS, Prosp.
            60-Let Oktyabrya, Bld 7-1, Moscow 117312, Russia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.8-beta
            Genome Coverage                   :: 158.0x
            Sequencing Technology             :: Illumina; Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_019787625.1-RS_2024_03_31
            Annotation Date                   :: 03/31/2024 06:12:30
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,448
            CDSs (total)                      :: 3,336
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 112
            rRNAs                             :: 7, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 222
            CDSs (without protein)            :: 222
            Pseudo Genes (ambiguous residues) :: 0 of 222
            Pseudo Genes (frameshifted)       :: 80 of 222
            Pseudo Genes (incomplete)         :: 138 of 222
            Pseudo Genes (internal stop)      :: 46 of 222
            Pseudo Genes (multiple problems)  :: 39 of 222
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP080639.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..8227
                     /organism="Acinetobacter lwoffii"
                     /mol_type="genomic DNA"
                     /strain="EK30A"
                     /isolation_source="permafrost, Kolyma lowland"
                     /db_xref="taxon:28090"
                     /plasmid="pALWEK1.5"
                     /geo_loc_name="Russia"
     gene            191..541
                     /locus_tag="ABEKA_RS16930"
                     /old_locus_tag="ABEKA_3517"
     CDS             191..541
                     /locus_tag="ABEKA_RS16930"
                     /old_locus_tag="ABEKA_3517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004644745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3147 family protein"
                     /protein_id="WP_004644745.1"
                     /translation="MVWLIAKYALTAGMVILISEVAKRSDRLGGLIAALPIVTILVLIW
                     MKLENQDPQKISNHAWYTFWYVVPTLPMFLAFPILYQRYGFWLSLAICYVMTILLFLAW
                     AAILHHFGIDLL"
                     /name="DUF3147 family protein"
     gene            complement(748..891)
                     /locus_tag="ABEKA_RS16935"
                     /old_locus_tag="ABEKA_3518"
     CDS             complement(748..891)
                     /locus_tag="ABEKA_RS16935"
                     /old_locus_tag="ABEKA_3518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004786602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_005166864.1"
                     /translation="MPKLKDIVLGIVVAPVLIPIMLIASYQDKKALKKDLDKRQKEKDQ
                     AS"
                     /name="hypothetical protein"
     gene            complement(911..1465)
                     /locus_tag="ABEKA_RS16940"
                     /old_locus_tag="ABEKA_3519"
     CDS             complement(911..1465)
                     /locus_tag="ABEKA_RS16940"
                     /old_locus_tag="ABEKA_3519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004282116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid replication DNA-binding protein"
                     /protein_id="WP_171264942.1"
                     /translation="MAKLSLSEVSNRFNVSRSTLYRAIKEGRISRSADGLFDIAEVIRC
                     FGEPIKKHEKKQEVDQSKDDADLRQLVDFMKKEIESYKDREKRYLDQIDRFQLLLGHKE
                     SEEKKSHDAPMKQSNDAPCGNSNETNEAIDNNKDIEINKSQETLEIHPNETPRHIPNTH
                     YETKKKRSLFGRVLNAVFDND"
                     /name="plasmid replication DNA-binding protein"
     gene            complement(1458..2414)
                     /gene="repM"
                     /locus_tag="ABEKA_RS16945"
                     /old_locus_tag="ABEKA_3520"
     CDS             complement(1458..2414)
                     /gene="repM"
                     /locus_tag="ABEKA_RS16945"
                     /old_locus_tag="ABEKA_3520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004282117.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein RepM"
                     /protein_id="WP_005166868.1"
                     /translation="MKNDLVVKDNALINASYNLEVTEQRLILLSIIRARETGQGISSDS
                     KLEIHASDYASRFDVTKEAAYNALKNAVNNLFERKFSFKEIHKDTNKEIVVKSRWVSRI
                     AYIDDLATLEVTFAPDVVPLITRLEKHFTSYQLKQVAQLTSKYAIRLYEFLIAWRNVGK
                     TPVISLSDFREKLGLEINEYQKMVNFKSRVLEPAIKQINELTDIHVKYEQYKTGRSISA
                     LSFTFKHKKIDSIPSERDPNTVDFFTQMTDAQRHMFANKLSELPEMGRYSQGTESYPQF
                     AIRIAEMLQNPNRIKELYPYLKKVGYMPSSKKDVVNG"
                     /name="repM"
     gene            complement(3731..4273)
                     /locus_tag="ABEKA_RS16950"
                     /old_locus_tag="ABEKA_3522"
     CDS             complement(3731..4273)
                     /locus_tag="ABEKA_RS16950"
                     /old_locus_tag="ABEKA_3522"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014505.4"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0003723 - RNA binding [Evidence IEA]; GO:0004521 - RNA
                     endonuclease activity [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="WP_171264944.1"
                     /translation="MSRLQQTLNTLKSKIERNFDSKRIDLLADWLHSWGYYIDQESSFD
                     STKRRNYAAGDIVSICLGFNIGSEQGGNRPALVIGRSSRKSKTILVVPLGSLDQTQTEK
                     DLRHFECFLGELKDFNLLAGKPAGTNSKAIINQIRTISKQRIIKPTSNSEACVNIGPEK
                     LKLVEEALIKSIKTIDI"
                     /name="type II toxin-antitoxin system PemK/MazF family
                     toxin"
     gene            4381..4977
                     /locus_tag="ABEKA_RS16955"
                     /old_locus_tag="ABEKA_3523"
     CDS             4381..4977
                     /locus_tag="ABEKA_RS16955"
                     /old_locus_tag="ABEKA_3523"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_171264945.1"
                     /translation="MLNVPAVCSCGLVFPSGIVLGGNVINLTLKGNKSRCPRCGNFADI
                     PDAVLNSYDGAVEVLQSSTYSESQITKFFSILQKTYSDLQDNKPVSLSDTVESLEDNIP
                     FLAALLPSLSNKAELYTFIGLLLQCLSMLIGNEPQTVNNFNGPVIINNYTSPELQVDRN
                     CIPSKPKQNISGMCICGSGLLSSLCCSNPTAKNYF"
                     /name="hypothetical protein"
     gene            complement(5001..5696)
                     /locus_tag="ABEKA_RS16960"
                     /old_locus_tag="ABEKA_3524"
     CDS             complement(5001..5696)
                     /locus_tag="ABEKA_RS16960"
                     /old_locus_tag="ABEKA_3524"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_222110756.1"
                     /translation="MKVDELLKTRQNRNLRGYKCMDNFIKMSGPCIDMAEKISQKKYEY
                     ATDFLMERSIIITYVSAVEVFYKDYLNLILHICKKDFVQKNLKELHQNKYDINDLIQIY
                     KHEINPYELVSSSISFQNIESIDKVFSKFLNKKGFWTSLDGMKIRFNKDDNKQAHFQPE
                     YINSLKNIFQLRHELVHDPAKTNFLNENIIKDLDNSIFCIIFSNIILNNIINDNLETNR
                     DSLNHINLA"
                     /name="hypothetical protein"
     gene            complement(5955..7304)
                     /gene="chrA"
                     /locus_tag="ABEKA_RS16965"
                     /old_locus_tag="ABEKA_3525"
     CDS             complement(5955..7304)
                     /gene="chrA"
                     /locus_tag="ABEKA_RS16965"
                     /old_locus_tag="ABEKA_3525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001060713.1"
                     /GO_function="GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_process="GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate efflux transporter"
                     /protein_id="WP_005098231.1"
                     /translation="MNNKSSLIDETEIESISFWQAFKFWLKLGFISFGGPAGQIAVMHQ
                     ELVEEKKWISEQRFMHALNYCMLLPGPEAQQLATYIGWLMHKTKGGIVAGVLFVLPSLF
                     ILIGLSWVYVKYGDVPIIAGLFYGIKPAVAAIVFHATYRIGSRSLKNKFLWAIAVIAFL
                     AIFIFNIPFPLIVLCAAISGYLVGKKYPDLFKLNAHQSSQKSYGRAIIDDDTPTPEHAV
                     FKWSHLIKITVISALLWAIPLISLTAVLGWSHSYTQLAWFFTKAALLTFGGAYAVLPYV
                     YQGAVGFYHWLTPTQMIDGLALGESTPGPLIMVVAFVGFLAAYNHSLLGAEHQFIAGAL
                     GAIIVTWFTFIFSFFFILAGAPVIESTHNQIKFTAPLTAITAAVVGVILNLALFLCYHV
                     LWPQGFEGNFDYISAIIAILAVVALFKFNQSVLRVIIGSALVGLTIKILL"
                     /name="chrA"
     gene            complement(7301..8227)
                     /locus_tag="ABEKA_RS16970"
                     /old_locus_tag="ABEKA_3526"
     CDS             complement(7301..8227)
                     /locus_tag="ABEKA_RS16970"
                     /old_locus_tag="ABEKA_3526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004649056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate resistance protein ChrB
                     domain-containing protein"
                     /protein_id="WP_005098232.1"
                     /translation="MKISLLISSLPSQSTTARMRIWRSLKSNGAATLRDGVYLLPEAHS
                     EKFESVVHDHLNEHGHAYIFQTEPPAHLDLAQLFDRTGEYEELRTEISQIQNTLEENKK
                     KEHLKQIRKLRKNLNSLIEIDFFPNTHQSKTLNELTRLEQVIARLGEYDEPNNFEQQLK
                     VLCKQDFQNKIWATRKRPWVDRLASAWIIQKFVDPQAKFIWLEHPNDCPKDTLGFDFDD
                     AQFTHINHLVTFEVLMHSFELQTPALNKIAEIVHFLDVGGNEPAEALGIEKILQGLRST
                     ITDDDQLLHLSNHIFDGLYADFQRNLT"
                     /name="chromate resistance protein ChrB domain-containing
                     protein"
     Typing          complement(1459..2414)
                     /name="rep_cluster_1154"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 gttataaaat caacaaattt tgtttatctt ataacaattt taaaggatta aaaatagttt
       61 tcctttaata agaatatatg taaaagtcat aaaagatttt tctatccttt aatagtaaat
      121 tcaacagatg cttataactt agcagtttgt aaaatagaac tttataaatc tactcatcta
      181 ggaaatgcac atggtctggc ttattgctaa gtacgctcta actgctggaa tggtcatact
      241 tatttcagaa gttgctaaac gtagtgaccg tctaggcgga ttgattgcag ccttacctat
      301 cgtaacgata cttgtgctga tatggatgaa acttgagaat caagacccgc agaaaatttc
      361 aaatcatgct tggtatacct tttggtatgt agtaccgaca ttacctatgt ttttagcatt
      421 cccaatactt tatcagcgtt atggattttg gctatctcta gccatttgct acgtaatgac
      481 cattcttctt tttcttgcat gggcagctat attgcaccat ttcggtattg atttgctgta
      541 aatgaactag gggcatgaat gttagttttt ctaaaattaa cataatacac cttatacgaa
      601 atgctctttt tattccttta aaaatcaatt tcttatatac tattaaaaag cccattcccc
      661 ctcgaatggg ctttttttat gattattcat gttccacgct tacttattga gcaaagttcc
      721 acggctttga taagcccagg tgaataatca ggaagcctgg tctttttctt tttggcgttt
      781 atctaggtct ttctttagcg cctttttatc ctggtaagac gcaatcagca tgatcgggat
      841 cagtacagga gcaaccacaa tacccagaac aatatctttc aattttggca tgatccttct
      901 cccttatttc tcagtcatta tcaaatacgg cattcagcac ccggccaaat aaactacgtt
      961 ttttctttgt ctcataatgt gtatttggta tgtgtcttgg tgtttcatta ggatgtattt
     1021 ctaatgtttc ttgagattta ttgatttcaa tatctttatt attatcaata gcttcatttg
     1081 tttcattact attgccgcat ggtgcatcat tggattgttt cattggtgca tcatgagatt
     1141 ttttttcttc agactcttta tgccctaata acaattgaaa gcggtctatt tggtctaaat
     1201 agcgtttctc acgatcttta tatgactcaa tctccttttt cataaaatca acaagctgac
     1261 gtaaatctgc atcatcctta gattgatcaa cttcctgttt tttttcatgc tttttgattg
     1321 gctctccaaa gcatcggatg acttctgcaa tatcaaaaag accatcagcg cttcgagata
     1381 tacgcccttc ttttatggct ctatataacg tagagcgact gacgttaaag cgattggata
     1441 cttcacttag tgataactta gccatttact acgtcctttt tacttgatgg catatatccc
     1501 acttttttta ggtacgggta tagttctttt attcggttag ggttttgcag catctcagca
     1561 atacgaatag caaattgcgg atagctttca gttccctggg aataccgccc catttcgggt
     1621 agctctgaaa gcttatttgc aaacatgtga cgttgtgcat cagtcatttg ggtaaagaaa
     1681 tctaccgtgt ttggatctct ttctgaaggt atcgaatcaa tttttttatg cttaaaagtg
     1741 aatgataagg ctgagataga tcttcctgtt ttgtactgtt catattttac atgtatatca
     1801 gtgagctcat tgatttgctt tatggctggt tctaaaacac gacttttaaa attaaccatt
     1861 ttttgatatt cattaatctc aagtcctaat ttttctctga agtcagataa agaaattaca
     1921 ggggtttttc ctacatttct ccaagcaatt agaaactcat ataaacgaat agcatatttg
     1981 ctagttaact gggcaacctg tttaagttga tagctcgtaa agtgcttttc caatcttgtg
     2041 ataagaggaa caacatctgg tgcaaaggta acttctaatg tagcaaggtc atcaatatat
     2101 gcaattcggc taacccagcg ggatttgact acaatttctt tgtttgtatc tttatgaatt
     2161 tccttaaaag agaactttct ttcaaaaaga ttattaactg cattttttag agcgttatac
     2221 gctgcttctt tggtgacatc aaagcgagaa gcataatcag aagcatgaat ctctaattta
     2281 ctgtcagatg agatcccttg gcctgtttct cttgctctta ttattgataa aaggataagc
     2341 cgttgctcgg ttacttcgag attataactg gcattaatta aggcattatc ttttactact
     2401 aagtcatttt tcataagact gcataggtta taagtgttat taagactaca ttagcattcg
     2461 atgtaatcaa atgcaaagaa attgtagtct tatgacaaag aaattgtagt cttatgacaa
     2521 agaaattgta gtcttatgac aaagaaattg tagtcttata agccttgaaa gccttgcaag
     2581 aaagggcttt cagcatctct aaaagcattt aaaaacttta aaataattaa aagcacaggg
     2641 aatgaaaaat tgcccttggt tttcgctacg ctcaaactct attgaatctc gcttacgctc
     2701 gattcgttgg ggcaattttt ggctcaatcc tggaaatgat ttaaatatta gtaagagcaa
     2761 agtcaaaacg ataagaacaa atggattcgc ttcgctcata gagctttttt gctcgcttcg
     2821 ctcgatccag gatcaaattt tcatatgtaa ttaaaaagtt tttagattct aatatttaaa
     2881 agtctgtttt tcagagagtc taagcacgaa tttcagcgat ttcactcgta gacactcgtt
     2941 ctgtaccttt cactgtgaat ttgagctcaa atcgcaaatg agtgcattta gaagctaagt
     3001 tttataggga ttagtaaagt ttttaccttt tgtatggggg ccatatatgc ctgttcatgc
     3061 attttcagga tcttttcttt tgattgaaga acgtatcttt taacttggtg acgtccagcg
     3121 atgatttgtt tctacttcat catagatttt taataagtag gattatctaa gattttttag
     3181 gaatatttga gaatttttag gaatttctag gaatatttga gaatttctaa gaatttttaa
     3241 gaatacgtgg aatttagggt tttgggcctg tttgaatagt cgatttttag gaatttttaa
     3301 gaatttttaa gaatttttaa taaagtactt aagggtaaag taggcaagat gtgtaatgtt
     3361 gaccaacatg caagcatgat tggcccacca ttacgcccac ttattcgctt tcagctcaac
     3421 gtggcatctt gtcctccgga gcttttggca tagccaaaat tgatacgcca aaagatcatc
     3481 aaaagaggtg taatttaaaa aagtgtaagg tgtgtgacct tgtatagtgg cgttacaccg
     3541 ccacttcgta taagagattt tatgttaaat agaacttatt agatttataa ggcaaaaaaa
     3601 acgccactgt tagatggcgt tttcttcttt cttaactaaa gctaagaaat gtgtcttttt
     3661 tggattagtt ctaagactaa tcaaggtgat aataatatta tactattaga agtttttgta
     3721 aagttaaatt ttatatgtct atagttttta tagactttat taacgcttct tctaccaatt
     3781 ttaatttctc tggacctatg ttcacacatg cttcagagtt gcttgttggt ttgataattc
     3841 gttgtttact aatagtccta atttgattaa tgattgcctt agaattcgtg ccagcaggct
     3901 tgcctgccaa taagttaaaa tccttcaatt cacccaaaaa acactcaaaa tgtcgcaaat
     3961 ctttttcagt ttgtgtttga tctaaagatc ctaaaggtac tactaaaatg gtcttacttt
     4021 ttctagaact tcgcccaatc actagagctg gtctatttcc accctgttca gatccaatgt
     4081 taaaaccaag gcaaatagat acgatatctc ctgcagcata atttcgtctt ttagttgaat
     4141 cgaaagagct ttcttgatca atataatatc cccaagagtg taaccaatca gccaatagat
     4201 ctattctttt agagtcaaaa ttcctttcaa ttttactttt caatgtattt aaggtttgtt
     4261 gtagacgcga catattagaa agattctttt ggtacaagac atagttaaat attctacaga
     4321 agaattgatt aatttattaa cgtaagtaat tatattcttt tatattagtt gattgtaaac
     4381 atgctaaatg ttcctgcggt atgttcttgt ggtctagttt ttccttcagg cattgttttg
     4441 ggtggaaatg ttataaatct tacccttaaa ggaaacaagt caagatgccc taggtgtgga
     4501 aattttgcag atattcctga tgctgtattg aattcatatg atggtgcagt agaagtatta
     4561 caatctagta cttattctga gtctcaaata actaaatttt ttagcatatt gcaaaaaaca
     4621 tattctgatt tacaagataa taagcccgta tctttaagtg atactgttga aagtttagaa
     4681 gataatattc cttttctagc cgctctatta ccctcattaa gcaataaagc cgaactttac
     4741 acatttattg ggctgttatt acagtgctta agtatgttga ttggtaatga gcctcaaact
     4801 gttaataact ttaatggtcc tgttattatc aataattata ctagtccaga gcttcaggtt
     4861 gataggaact gtattccatc aaaacctaag cagaatattt ctggtatgtg tatatgtgga
     4921 tctggcttac ttagttcttt atgttgttca aatcctacag ctaaaaatta tttttaataa
     4981 aaccaagcgt aagttaattc ttacgctaaa ttgatatgat ttagtgaatc tctatttgtc
     5041 tcaaggttat catttatgat gttatttaaa atgatgtttg agaaaattat gcaaaaaatt
     5101 gagttatcta agtctttgat tatattttca tttaaaaaat ttgttttagc tggatcatgt
     5161 actaactcat gtcttaactg aaaaatattt ttaagactat ttatgtattc aggctgaaag
     5221 tgagcttgtt tattatcatc tttattaaac ctaattttca tgccatcaag actggtccaa
     5281 aaaccttttt tatttaaaaa tttagaaaaa actttatcta ttgattctat attctgaaat
     5341 gaaatactgc tggatactaa ttcatatgga ttgatctcat gtttatagat ttgaattaaa
     5401 tcatttatgt catatttatt ttggtgtaat tccttcaagt ttttttgaac aaaatccttt
     5461 ttgcagatat gtaaaattaa attgagatag tctttataaa aaacttcaac agcacttaca
     5521 tatgtaatta taatggatct ttccataaga aaatcagtag catattcata ttttttttga
     5581 gatatttttt ctgccatatc aatacatggg cctgacattt ttatgaaatt atccatgcac
     5641 ttatagccac gtaggtttct attttgccgt gttttaagca gttcatctac tttcatatat
     5701 ttaactttct ctaaaattaa cataatacac gttatgcgaa attagaaaaa ttagtttatt
     5761 gcactgatat ttatctgttt tttaaatttt agacctgggt tggtgcccag gtttttttat
     5821 tgcgtataac agccattatg ttaaatgggg agtgatttag tggaatattc tttaattttt
     5881 tatatgaaag aagatacctt gaatcgtgtc aaaagaagca tagatttttt atccatacct
     5941 ctagtgttca ttctttatag taaaatttta atcgttaaac ccactaaagc agaaccgatg
     6001 atgactctga gcacactttg attaaattta aataatgcga ctacagctaa aattgcaata
     6061 atggcagaga tataatcaaa attcccttca aaaccctgtg gccaaagcac gtggtaacat
     6121 aaaaagagtg ccaaatttaa aattacgccc accacggcag cggtaatggc ggttaaaggg
     6181 gcagtaaatt taatttgatt gtgtgtggac tctataacag gtgctccggc taagataaag
     6241 aaaaatgaga aaataaaagt aaaccacgtg acgatgattg cacctaatgc acctgcaata
     6301 aattgatgtt cagcacccaa cagcgaatgg ttataagctg ctaagaagcc gacaaaagcc
     6361 accaccatga tcaatgggcc gggagtactt tcacctaggg ctaaaccatc gatcatttga
     6421 gttggggtga gccagtgata aaaaccgaca gcgccttgat aaacgtaagg cagaacagca
     6481 taagcaccac caaatgtcag aagtgcagct ttagtaaaga accaagccag ttgagtataa
     6541 gaatgactcc atcccaaaac tgctgttaaa gagatgagtg gaattgccca taaaagtgct
     6601 gaaattacag ttattttgat cagatgagac catttaaaaa cagcatgttc aggtgtaggg
     6661 gtatcatcat caataattgc acgtccatag gatttttgac tggactgatg tgcatttagc
     6721 ttaaataaat caggatactt tttaccgacc aaataacccg atatcgctgc acataaaacg
     6781 attaaaggga aaggaatatt aaaaataaat attgcaagaa aagcgataac tgcaatcgcc
     6841 catagaaatt tattctttaa tgaacgactg ccaatacgat acgtggcatg aaatacaatt
     6901 gcggcaactg cgggcttaat gccataaaac aaaccggcaa tgatcgggac gtcgccatat
     6961 ttcacataga cccaagacag cccaatgagg ataaaaagag aaggtagaac aaataaaacc
     7021 cctgcaacga tgccaccttt ggttttatgc attaaccagc caatataagt tgcaagttgt
     7081 tgagcttcgg ggccgggtaa gagcatgcaa taattcaatg cgtgcataaa acgctgttct
     7141 gaaatccatt ttttttcttc aactaactct tggtgcataa cagcgatttg cccggcaggg
     7201 ccaccaaagc taatgaatcc cagttttaac caaaatttga atgcttgcca aaagctgata
     7261 gattcaattt ctgtttcatc tattaaagat gacttattat tcatgttaaa ttcctttgaa
     7321 agtctgcata taagccatca aaaatatggt ttgaaagatg gagtaattga tcatcatctg
     7381 tgatcgtgct tcttaagcct tgcaagattt tttcaatgcc taaggcttcg gcaggctcat
     7441 ttcccccaac atctaaaaaa tgaacaattt cagcgatttt atttaaagca ggggtttgta
     7501 attcaaagct atgcattaac acttcaaaag tgactagatg attgatatgg gtaaattggg
     7561 catcatcaaa atcaaaacct aaggtatctt tggggcaatc attgggatgc tctaaccaaa
     7621 taaattttgc ctgagggtct acaaatttct gaatgatcca tgccgatgcc aaacggtcaa
     7681 cccaaggacg ctttctggtt gcccagattt tattttgaaa atcttgcttg cataggactt
     7741 ttaattgctg ttcaaaatta ttgggttcat cgtattcacc taaccgggca ataacttgtt
     7801 ctaatcgggt gagttcattc agtgttttgc tttgatgagt atttgggaaa aaatctattt
     7861 ctatcagtga attgaggttt tttcttaatt ttcgaatctg tttgagatgt tcttttttct
     7921 tattttcctc aagcgtattt tgtatttgtg aaatttcagt tctcagttct tcatattctc
     7981 cagtcctgtc gaaaagttga gcaaggtcta aatgtgcagg aggttcagtc tgaaagatat
     8041 aggcatgccc atgttcatta agatgatcat gaacaacaga ctcgaatttt tcagaatgtg
     8101 cttcgggtaa gaggtaaaca ccatctctta aagttgctgc accatttgac tttaatgaac
     8161 gccaaattcg cattcttgcg gttgtgcttt gactgggaag tgatgagatc agtaatgata
     8221 ttttcat
//
