LOCUS       NZ_CP080638             4181 bp    DNA     circular CON 31-MAR-2024
DEFINITION  Acinetobacter lwoffii strain EK30A plasmid pALWEK1.17, complete
            sequence.
ACCESSION   NZ_CP080638
VERSION     NZ_CP080638.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN05251105
            Assembly: GCF_019787625.1
KEYWORDS    RefSeq.
SOURCE      Acinetobacter lwoffii
  ORGANISM  Acinetobacter lwoffii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Acinetobacter.
REFERENCE   1  (bases 1 to 4181)
  AUTHORS   Rakitin,A.L., Ermakova,A.Y., Beletsky,A.V., Petrova,M.,
            Mardanov,A.V. and Ravin,N.V.
  TITLE     Genome analysis of Acinetobacter lwoffii strains isolated from
            permafrost soils aged from 15 thousand to 1.8 million years revealed
            their close relationships with present-day clinical isolates
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4181)
  AUTHORS   Rakitin,A.L., Ermakova,A.Y., Beletsky,A.V., Petrova,M.,
            Mardanov,A.V. and Ravin,N.V.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2021) Institute of Bioengineering, Research Center
            of Biotechnology of the Russian Academy of Sciences, Moscow 119071,
            Russia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.8-beta
            Genome Coverage                   :: 158.0x
            Sequencing Technology             :: Illumina; Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_019787625.1-RS_2024_03_31
            Annotation Date                   :: 03/31/2024 06:12:30
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,448
            CDSs (total)                      :: 3,336
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 112
            rRNAs                             :: 7, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 222
            CDSs (without protein)            :: 222
            Pseudo Genes (ambiguous residues) :: 0 of 222
            Pseudo Genes (frameshifted)       :: 80 of 222
            Pseudo Genes (incomplete)         :: 138 of 222
            Pseudo Genes (internal stop)      :: 46 of 222
            Pseudo Genes (multiple problems)  :: 39 of 222
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP080638.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4181
                     /organism="Acinetobacter lwoffii"
                     /mol_type="genomic DNA"
                     /strain="EK30A"
                     /isolation_source="permafrost, Kolyma lowland"
                     /db_xref="taxon:28090"
                     /plasmid="pALWEK1.17"
                     /geo_loc_name="Russia"
     gene            join(2761..4181,1..559)
                     /locus_tag="ABEKA_RS16915"
     CDS             join(2761..4181,1..559)
                     /locus_tag="ABEKA_RS16915"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="WP_222110754.1"
                     /translation="MIVQFFRYGNGLSKGPLDYLLGKDRDRDYAKVLQGDVSEVSGLID
                     TSPYAKKYTSGCLSFYEHDLSDQDKQKIMQDFEQALFPGMDQSQYRVLWIEHQDKLNEE
                     TGERRLELNFLIPNVEIHTGQRLQPYYDKADRPRIDLFKKITNYEYQLHDADDPLYRQA
                     VTTAKNLPKTVNEIKETLDIEATRAVESGLITDRQSMKKWLLDLGLEVTRETKKSLSIK
                     NPNDDEKARPIRLTGAIYEQDFRLTETSQQLTIAASERYRREAEQRNQSDIQRYAEYSA
                     KRSTELEQQYRQHQNGYTAETERSNERDIAADRPDYSSSQEAAATRHDSPIARTTTADQ
                     TATSELAAVERLEPRNSPSSQIKENPYYIEYSLDFTGMYNAYQQYLSGIRQQEQIQRYT
                     SDAEYSRLSKITGGKPDDNNMRWQAVRPNRPELYEQFIRNTRYETTANDREGGALNESR
                     SAVIEHYRRTAESIESRSAVIEHYRRTAESIEATTARARESTATHSTAIRDYRAVKELH
                     EHFKQQASGSSEDSAEISAVRARTVRANYLSNFFTRCTTELESTVADTFKRFSAELADR
                     QSSQKSGIEDYSASSRIGDQQSIETTSRTNHRENEFSRAVSAEISSINPTSVIKALDVL
                     DQRKELQREQERQRSRDYDSPSPF"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            857..2278
                     /locus_tag="ABEKA_RS16920"
                     /old_locus_tag="ABEKA_3323"
     CDS             857..2278
                     /locus_tag="ABEKA_RS16920"
                     /old_locus_tag="ABEKA_3323"
                     /inference="COORDINATES: protein motif:HMM:NF015076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_222110755.1"
                     /translation="MLKNVTAFNLRKKALIFEEQQTKKSDLDASLSVFRLSIPDNCPLW
                     TSKIALAGEKPTWFHKDEANFADFAYLKTATDFKVGTLIIDIDKPIADHIEALIYGSLN
                     APIPNLIIKNRANKHVQLVYCLKNWVCIDVRNTKGNYSAKAHSLYKALKRHLNGIFGGD
                     DAYHNFIAKNPFCEQWDVIGMRSTSYEMYELARISELEVKSTTFIERLQSKQTDKALKQ
                     ASDRATVGSGERNDFLFNTVRVRAYSLYNKHSKDWTRQQFENCINDFAQELNQTKCSPA
                     LEISEVATICHSIVSFCYGSFGEHQKYTKSEISEIASKCGRKGGKAKGYKYAQQRQQAR
                     KLAKQGKKAKDIAVITGLSIRAVYNAINEEKPLKKSVQKPTEKNIKYHVFNPLFCANSY
                     QCSVLLVKELRSNSHLVCERSEPKLFFKPLSLKISNTPTVKHPNIHIPFVLLPQITQHQ
                     IPQSQIAFVSCKQHS"
                     /name="replication initiation protein"
     gene            2420..2764
                     /gene="mobC"
                     /locus_tag="ABEKA_RS16925"
                     /old_locus_tag="ABEKA_3324"
     CDS             2420..2764
                     /gene="mobC"
                     /locus_tag="ABEKA_RS16925"
                     /old_locus_tag="ABEKA_3324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004993722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="WP_111810824.1"
                     /translation="MTETKRNKVYKVRFSDAEVQKIKDANILNIAKYLRDTSLAQIDAK
                     QGNVKKKNQEHHYTKLDREFLLELSRIGGNINQIAKAINTDIASSRPLDAARLLHLLIG
                     IDQTLKELKK"
                     /name="mobC"
     Typing          2981..3303
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="88.889"
                     /coverage="100.0"
                     /evalue="2.1700000000000007e-59"
ORIGIN
        1 tagaagcgca gttattgaac attatcgaag aacagcagaa tcaattgaag caacaacagc
       61 aagagcaaga gaaagcactg caactcattc aacagcaatc agggactatc gagcagttaa
      121 agagctacac gaacacttta aacagcaagc atcaggaagc agtgaagata gtgcagaaat
      181 atcagcagtt cgtgcaagaa ctgtcagagc aaactatctc agcaactttt tcacaagatg
      241 tacaacagaa cttgaaagta cagttgcaga cacgtttaaa cgatttagtg cagaacttgc
      301 agatcgacaa tcaagtcaaa aatcaggtat cgaagattat tccgcaagca gtcgaattgg
      361 agatcaacag tcaattgaaa ccactagcag aacaaatcac cgagagaatg agttcagtag
      421 agcagtttca gcagaaatta gctcaattaa tccaacaagt gtcatcaaag ctttagacgt
      481 gcttgatcaa cgtaaagagc tacagagaga gcaagagcgt caacgcagtc gagattatga
      541 ttcaccaagc ccattttgac ccattttttt tgcggagcat tgcaggtttt gatgtggtaa
      601 cagatttatt tttgcgaatt ttcaacgaat gagaaaaaaa atcttgcaat agttgtagag
      661 taacttcaaa ttgagagcaa ttagatacat tttcttgaat tagaaaaagt atcgaccaaa
      721 aaaaagccct cttatcttgg cggacaatgg cttttttttg gcttcatcag tcgacttgtt
      781 agtaactgaa tccacaatgt atagcaatat tatgcggtgg atacagtgaa ttgacaagtc
      841 ttacagcaga cttgttatgc taaaaaatgt gacagcgttt aatttacgca aaaaagccct
      901 gatttttgaa gaacaacaga ctaagaagtc tgatcttgac gcatctctta gcgtttttcg
      961 tctttcaatc cccgataatt gtccactttg gacttcaaaa atagctcttg caggtgaaaa
     1021 accgacttgg tttcacaaag atgaggctaa ttttgcggat ttcgcatatt taaagaccgc
     1081 tacagatttt aaagtcggca cattgatcat agatatagac aaacccattg cagaccatat
     1141 tgaagccctc atttatggct ctttaaatgc cccaattcca aatttaatca ttaaaaatcg
     1201 agctaataag catgttcagc tcgtctattg tttgaaaaac tgggtctgta ttgatgtgcg
     1261 gaatacgaaa ggcaactata gcgcaaaagc gcacagcctc tataaagccc ttaaacgcca
     1321 tttaaacggc atttttggcg gtgatgatgc ttatcataac tttatcgcta aaaatccatt
     1381 ctgtgagcaa tgggacgtaa tagggatgcg ttcaacaagc tatgagatgt atgaacttgc
     1441 tcgaatttca gaactagaag tcaaaagcac aacattcatt gagcgattac agtcgaagca
     1501 aacagataaa gccctgaaac aagcgtcaga tcgtgcaact gttggaagcg gtgaacgtaa
     1561 tgacttctta ttcaatactg tgcgtgtacg tgcttattca ctctataaca agcattcaaa
     1621 agattggaca cgtcaacagt ttgagaattg cattaatgat tttgctcaag aactaaatca
     1681 aacgaaatgc agtccagcgc ttgaaattag tgaagttgct acgatttgcc atagcatagt
     1741 gagcttttgt tacggctcgt ttggtgaaca tcagaaatat acgaaatcag aaattagtga
     1801 gatagcgtca aaatgtggac gaaagggtgg taaagcaaaa ggctataagt acgcacaaca
     1861 acgccaacaa gctcgtaaac tagctaaaca gggcaagaaa gctaaagata tagctgtgat
     1921 tactggttta tcaattcgtg ctgtctacaa tgcaattaac gaagaaaaac cgctaaaaaa
     1981 atcagtgcaa aaaccgactg aaaaaaatat taaatatcat gtatttaacc ccctattttg
     2041 tgcaaatagc tatcagtgta gtgtgctcct ggtaaaagaa ttacgtagta attcacatct
     2101 tgtatgcgag cgaagcgagc caaagctttt tttcaagcct ttaagcctta aaatttcaaa
     2161 tactccaact gttaaacatc cgaatattca tattcctttt gttctgctcc ctcaaataac
     2221 tcaacatcaa ataccgcaat cacagatagc ctttgtttcg tgtaaacaac attcttgatg
     2281 aatgattgtt taacacaaaa caagatgcta ttgtgattta acgcaaggat acatagagac
     2341 ccctccactt cgttgcaggc ttctatgacc ttgttcagga ggagacctcc cgaaaccctc
     2401 caaaagcaaa ggcaaaagca tgacagagac caaacgtaat aaagtttata aagtccgatt
     2461 ttctgatgct gaagtacaga aaatcaaaga tgccaatatc ctaaatattg cgaaatatct
     2521 aagagatact tcgcttgccc aaattgatgc aaagcaaggc aacgtcaaaa aaaagaatca
     2581 ggaacatcac tacaccaaat tagatcgtga atttttactt gagctatctc gaattggggg
     2641 caacatcaat cagattgcaa aggcaataaa taccgatatt gcaagcagtc gtccccttga
     2701 tgctgcaagg cttttgcacc tgttaatagg gattgatcag accttaaagg aattgaaaaa
     2761 atgattgttc agttctttag atacggcaat ggtttgagca aaggtccact ggattattta
     2821 cttggtaaag accgtgatcg agattatgca aaagtcctac agggcgatgt ttccgaagta
     2881 tcaggattaa ttgatacgtc accttatgca aagaaatata catccggatg cctaagtttt
     2941 tatgagcatg atttatctga tcaggataaa caaaaaatca tgcaggattt tgagcaagcc
     3001 ttattccctg gcatggatca gagccagtac cgtgtcttgt ggattgagca tcaagacaaa
     3061 ttaaacgaag aaactgggga gcgaagatta gagcttaatt ttttaatccc aaatgttgaa
     3121 attcatacag ggcaacgctt acagccttac tatgataaag ctgatcgtcc tagaattgat
     3181 ctgtttaaga agattacaaa ctatgagtat cagctgcatg atgcagatga ccccctctat
     3241 cgccaagccg ttacaacggc aaaaaacttg cctaaaactg ttaatgagat caaggaaacg
     3301 cttgatattg aagcaacgag agccgttgaa agtggactaa ttacagatcg gcagagcatg
     3361 aaaaaatggc tattggatct tggtcttgaa gtcaccagag aaaccaagaa aagcctatcc
     3421 attaaaaatc cgaatgatga cgaaaaagcc cgcccgataa ggcttacagg tgcaatctat
     3481 gagcaagatt ttagacttac agaaacgagc caacaactta ctatcgcagc atcagaacga
     3541 tatagacgag aagctgaaca gcgaaatcag tcagatattc aaagatatgc ggaatacagt
     3601 gcaaagcgaa gcacagagct tgagcagcag tatcgacagc accaaaacgg atatacagca
     3661 gaaacagagc gaagcaacga acgagatatt gcagcagatc gaccagacta cagctcaagc
     3721 caagaagcag cagcaacaag gcatgacagc cctattgcaa gaacaacaac agcagatcaa
     3781 acagctacaa gcgaacttgc agcagttgaa cggcttgagc caagaaacag cccaagcagc
     3841 caaattaaag aaaaccctta ttacatcgaa tatagccttg atttcactgg catgtataac
     3901 gcttatcagc agtatttgtc tgggatacgt cagcaagagc aaatacaacg atatacaagc
     3961 gatgcagaat acagtcgatt atctaaaatc acagggggga agcctgatga taacaacatg
     4021 cgatggcaag ctgtgcgtcc aaatagaccc gaattatatg aacaattcat acgtaacacg
     4081 agatacgaaa caaccgctaa tgatcgtgaa ggaggtgcat taaatgaatc tagaagcgca
     4141 gttattgaac attatcgaag aacagcagaa tcaattgaat c
//
