LOCUS       NZ_CP080637             8910 bp    DNA     circular CON 31-MAR-2024
DEFINITION  Acinetobacter lwoffii strain EK30A plasmid pALWEK1.13, complete
            sequence.
ACCESSION   NZ_CP080637
VERSION     NZ_CP080637.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN05251105
            Assembly: GCF_019787625.1
KEYWORDS    RefSeq.
SOURCE      Acinetobacter lwoffii
  ORGANISM  Acinetobacter lwoffii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Acinetobacter.
REFERENCE   1  (bases 1 to 8910)
  AUTHORS   Mindlin,S., Beletsky,A., Rakitin,A., Mardanov,A. and Petrova,M.
  TITLE     Acinetobacter Plasmids: Diversity and Development of Classification
            Strategies
  JOURNAL   Front Microbiol 11, 588410 (2020)
   PUBMED   33304332
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8910)
  AUTHORS   Rakitin,A.L., Ermakova,A.Y., Beletsky,A.V., Petrova,M.,
            Mardanov,A.V. and Ravin,N.V.
  TITLE     Genome analysis of Acinetobacter lwoffii strains isolated from
            permafrost soils aged from 15 thousand to 1.8 million years revealed
            their close relationships with present-day clinical isolates
  JOURNAL   Unpublished
REFERENCE   3  (bases 1 to 8910)
  AUTHORS   Rakitin,A.L., Ermakova,A.Y., Beletsky,A.V., Petrova,M.,
            Mardanov,A.V. and Ravin,N.V.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2021) Institute of Bioengineering, Research Center
            of Biotechnology of the Russian Academy of Sciences, Moscow 119071,
            Russia
REFERENCE   4  (bases 1 to 8910)
  AUTHORS   Mindlin,S.Z., Beletsky,A.V., Mardanov,A.V. and Petrova,M.A.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2020) Institute of Bioengineering, Research Center
            of Biotechnology of the Russian Academy of Sciences, Moscow 119071,
            Russia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.8-beta
            Genome Coverage                   :: 158.0x
            Sequencing Technology             :: Illumina; Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_019787625.1-RS_2024_03_31
            Annotation Date                   :: 03/31/2024 06:12:30
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,448
            CDSs (total)                      :: 3,336
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 112
            rRNAs                             :: 7, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 222
            CDSs (without protein)            :: 222
            Pseudo Genes (ambiguous residues) :: 0 of 222
            Pseudo Genes (frameshifted)       :: 80 of 222
            Pseudo Genes (incomplete)         :: 138 of 222
            Pseudo Genes (internal stop)      :: 46 of 222
            Pseudo Genes (multiple problems)  :: 39 of 222
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP080637.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..8910
                     /organism="Acinetobacter lwoffii"
                     /mol_type="genomic DNA"
                     /strain="EK30A"
                     /isolation_source="permafrost, Kolyma lowland"
                     /db_xref="taxon:28090"
                     /plasmid="pALWEK1.13"
                     /geo_loc_name="Russia"
     gene            join(8878..8910,1..435)
                     /locus_tag="ABEKA_RS16860"
     CDS             join(8878..8910,1..435)
                     /locus_tag="ABEKA_RS16860"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_202034684.1"
                     /translation="MQLSIENPSLDLIEKVCVDAKNNNFSTISEYLTYLVEQASETNEK
                     PNEDEIYLIINNLIDLALRNRRDLADFTMQELYTFCIKKEEEFDYPKWSNLDRSVRIDL
                     GKKFKAAILKHADKIEEGEYYIKQDGTNINNSALYRVVRDRVSLSRPLHRL"
                     /name="hypothetical protein"
     gene            complement(580..756)
                     /locus_tag="ABEKA_RS16865"
                     /old_locus_tag="ABEKA_3302"
     CDS             complement(580..756)
                     /locus_tag="ABEKA_RS16865"
                     /old_locus_tag="ABEKA_3302"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_202034685.1"
                     /translation="MNVRHSIDRRETGVKVVGHQFKIDENRMNETLRSPRFQAPQGNLT
                     VDSLVEFLKNRKK"
                     /name="hypothetical protein"
     gene            1260..1700
                     /locus_tag="ABEKA_RS16870"
                     /old_locus_tag="ABEKA_3304"
     CDS             1260..1700
                     /locus_tag="ABEKA_RS16870"
                     /old_locus_tag="ABEKA_3304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019767310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_202034687.1"
                     /translation="MSENTDEPPKWSIILNEKFGEEFLNYPRDQQEAVVDFMNMVQTHG
                     LENFDAFKGKGKIKPSWAPPAYLHSDNYEYAKTNNLWHYHLGVPFYEKGRGEFLTSDWL
                     LHFQWNQSHHHDKYNIYLADCSSHYRSNGIFYLPSQPYLIKK"
                     /name="hypothetical protein"
     gene            complement(2033..2257)
                     /locus_tag="ABEKA_RS16875"
                     /old_locus_tag="ABEKA_3305"
     CDS             complement(2033..2257)
                     /locus_tag="ABEKA_RS16875"
                     /old_locus_tag="ABEKA_3305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004676219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004758944.1"
                     /translation="MPYTEFQRLVGKAGLSIKEFAALLDMKPNSITNYSKQGVVPTHIA
                     VIVALISTMKDEGLDFYPIFEKVKSYSQE"
                     /name="hypothetical protein"
     gene            2411..2656
                     /locus_tag="ABEKA_RS16880"
                     /old_locus_tag="ABEKA_3306"
     CDS             2411..2656
                     /locus_tag="ABEKA_RS16880"
                     /old_locus_tag="ABEKA_3306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005249014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004282220.1"
                     /translation="MYSYPDSNTEKKIALMIINDFFIQKAHELWIFLQLDQCFNDYEAT
                     LIWTRRYLEKHPECEYSDIQKAFVSCFPEHFFSFDC"
                     /name="hypothetical protein"
     gene            3202..3963
                     /locus_tag="ABEKA_RS16885"
                     /old_locus_tag="ABEKA_3308"
     CDS             3202..3963
                     /locus_tag="ABEKA_RS16885"
                     /old_locus_tag="ABEKA_3308"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_104221582.1"
                     /translation="MQNSDFKKRGRPALLLNQLELKAYVDYVRKYKIWDQVTQNAVREI
                     QASQFEMQNLSPTALVIVKQAIKPFRELQKVELLYQQLCKTTSHDFFQKKFVELYQQYQ
                     STREDQTLNVLKTMATQYLRFKDNKVDENSLKLYLSQLEKKENKAKRNADNKKKFELGG
                     ALLALLKTKNIDVTKLTAAQMIRALFSQDMHFNLANCNTIIFQEMLNVGLSETQAKTLF
                     PLVLDQLTDYRDEDGLPIYLKQIIKTMAENG"
                     /name="hypothetical protein"
     gene            3956..5008
                     /locus_tag="ABEKA_RS16890"
                     /old_locus_tag="ABEKA_3309"
     CDS             3956..5008
                     /locus_tag="ABEKA_RS16890"
                     /old_locus_tag="ABEKA_3309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006582645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="WP_104221583.1"
                     /translation="MASYHLQLRDKPKGYALEHYFYIARLEKYQHVRVKSQEVLEHIEP
                     GGCMPSWAKDPADFWKAADTHERANAKVYMEYELALPNELTPEQRKTLVEAFLNEHVAS
                     HQYPHSYAIHNVKSRIGGIDQPHCHLMLSLKANDGLDRTPETYFKRFNPKNPEKGGAKK
                     RQLDEGFDNYRSFLLYIRKEWENYMNDALRQYAPTLTYQIDGQEINIPNRVSSSSYKQY
                     NLDHGTFYIAEPKLGVGNQNNTPEYLEQIKQIREHNQQEFEREQLQKHQELNLSAKPFY
                     SVNDQPYSSLEVTALLKSLNPDTHSVEITAIQQQQALVDPSFKNQELNLTPENKPTPTI
                     KKDTGFNFSM"
                     /name="MobA/MobL family protein"
     gene            5546..6490
                     /gene="repM"
                     /locus_tag="ABEKA_RS16895"
                     /old_locus_tag="ABEKA_3310"
     CDS             5546..6490
                     /gene="repM"
                     /locus_tag="ABEKA_RS16895"
                     /old_locus_tag="ABEKA_3310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798284.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein RepM"
                     /protein_id="WP_104221584.1"
                     /translation="MKNDLVVKDNALINASYNLEVTEQRLILLSIIRARETGQGISSDS
                     KLEIHASDYASRFDVTKEAAYNALKNAVNNLFERKFSFKEIHKDTNKEIVVKSRWVSRI
                     AYVDDLATLEITFAPDVVPLITRLEKHFTSYQLKQVAQLTSKYAIRLYEFLIAWRDVGK
                     TPVISLSEFRERLGLEMNEYQKMVNFKSRVLEPAIKQINELTDIHVKYEQYKTGRSISG
                     LSFIFKHKKIDSIPSERDPNTVDLFTQMTDAQRHMFANKLSELPEMGRYSQGTESYPQF
                     AIRIAEMLHDPEKIKELYPYLKKVGYMPLNKRI"
                     /name="repM"
     gene            6494..7048
                     /locus_tag="ABEKA_RS16900"
                     /old_locus_tag="ABEKA_3311"
     CDS             6494..7048
                     /locus_tag="ABEKA_RS16900"
                     /old_locus_tag="ABEKA_3311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004282116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid replication DNA-binding protein"
                     /protein_id="WP_104221585.1"
                     /translation="MAKLSLSEVSNRFNVSRSTLYRAIKEGRISRSSDGLFDIAEVIRC
                     FGEPIKKHEQKQEVDQSKDDADLRQLVDFMKKEIESYKDREKRYLDQIDRFQLLLGHKE
                     SEEKISHDAPVTHSNSAPCDNNSKISETIDNNRDSEIKTSHETLGTRPNETPKHVPNTH
                     YETKKKRGLFGRVLNAVFDND"
                     /name="plasmid replication DNA-binding protein"
     gene            7061..7204
                     /locus_tag="ABEKA_RS16905"
                     /old_locus_tag="ABEKA_3312"
     CDS             7061..7204
                     /locus_tag="ABEKA_RS16905"
                     /old_locus_tag="ABEKA_3312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004786602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001125247.1"
                     /translation="MPKLKDIALGIIVAPLLIPIMLIASYQDKKALKKELDERQKEKDQ
                     AS"
                     /name="hypothetical protein"
     gene            7494..8387
                     /locus_tag="ABEKA_RS16910"
                     /old_locus_tag="ABEKA_3314"
     CDS             7494..8387
                     /locus_tag="ABEKA_RS16910"
                     /old_locus_tag="ABEKA_3314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004282254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine exporter LysO family protein"
                     /protein_id="WP_063454738.1"
                     /translation="MINNLIFSLSPILLSLLLGWIVGKTSREALRFRLVSLITILVWLL
                     LISIGFQFGSVLFDRKLGSSILIEAFFYATILSLITFILLFRKNLISNDKKKSLEQIFK
                     PILECLIAIFMVVIGMSLYSILPQNINSELISSILLYILIFLVGVDLSSIKIQFLTLDH
                     IKVPIFTILALFISAYITTFFLEKSFIELVVLGSGFGWFSLSGPLVGKTLGVEQGTFAL
                     LVDLVREFYAIGLLYLLGRAYPNPIIGICGATAMDSTLPFIKNNCSQLNVQIAIFSGFI
                     LTLIAPFFIIIFSNFV"
                     /name="lysine exporter LysO family protein"
     Typing          5547..6501
                     /name="rep_cluster_1154"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.984"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 ttaattgaaa aagtttgtgt tgatgctaaa aataataatt tttccacaat ttctgaatat
       61 ttaacttatt tagttgaaca agcttctgaa accaatgaaa aacctaacga agatgaaata
      121 tacctaatta ttaataattt aattgatctt gcattaagaa atagaagaga tttagctgat
      181 ttcacaatgc aagagctata tacgttttgc ataaaaaaag aagaagagtt tgattatcct
      241 aaatggtcaa atttagatag aagcgtacga attgacttag gcaaaaaatt caaagctgct
      301 attttaaaac atgcagataa aattgaagaa ggtgagtatt acatcaaaca agatgggact
      361 aatatcaata actctgctct ttatagagtt gtaagagatc gagtttcttt aagtcgcccc
      421 cttcatcgtt tgtaaaagat tcacgacacc ctaatggtgt ctttttaaat ctgttttaaa
      481 aatatgattt attgaatcta gtggttattg tgaaaattta atcgtaaaaa aagcatggaa
      541 aattccatgc ttttttgtag gcagaaaagt gctttctact tatttcttac gattctttaa
      601 aaactcaact aagctatcaa ctgtcaagtt cccttgtggt gcttgaaaac gtgggcttct
      661 tagagtttca ttcatacgat tttcatcaat cttaaattga tgaccgacaa ctttaacccc
      721 agtctctctg cgatcaatgc tatggcgtac attcatatta gatatgtcct atacagactc
      781 ttacaagtga atttctttta tagctttaga taactatatg gaggcagtat atagttttaa
      841 aatacttaat aaatattttt tcgataaaaa gcaacaaaaa aaacttgaca aaaaattttt
      901 tttaaatata cttatttatt acaataatat ttaattttat actagtaatt gtttttaatc
      961 tgatttagct ttttttaaaa atcccaatca aattaaatgg ttaatatatg ttcaagtgta
     1021 ataatccctt ggcgttggtt gatgctttgt cttattcatt tccgaatgca gtcaaagaag
     1081 agccaacctt aggtaaagtc gtatttagaa ttgataaagt catatgcaat atctttctaa
     1141 ctacaggaac cgtgaacttc caaggtaagt ttgatggtcc aaataaagca actattgagt
     1201 ctatttcatt aataattgac tcaattaatt cggaatcata actatgtaaa ggagtaaaaa
     1261 tgtctgaaaa tactgatgaa ccaccaaagt ggtctattat tttaaatgaa aaattcgggg
     1321 aagaatttct gaattatcct cgtgatcaac aagaagccgt tgttgatttt atgaatatgg
     1381 tacaaaccca tggtttggaa aatttcgatg cctttaaagg caaagggaaa attaagccct
     1441 catgggcacc accagcttat ctccatagtg ataactatga atatgccaag actaataact
     1501 tatggcatta tcatctaggt gttccatttt atgagaaagg ccgtggtgaa tttttaacat
     1561 cagattggtt attacatttc caatggaatc agtctcacca tcatgataaa tataatatat
     1621 atttggcaga ctgttcatcc cactatagaa gtaacggaat tttctatctt ccatcccaac
     1681 cctatttaat aaaaaaataa ttttgaacag atgttaatgg ataatctatc gattaccatt
     1741 aacataacgt taatatccaa attctgattt cataaattta tttaacataa aatctcttat
     1801 gcgaaattca tctgtaagcc caaaatagaa atgtccggtt taccgaatgc tcgacctaag
     1861 atctcagaac ataagaattt gatgatcccc ctggttctca aaactcgata ctttattgaa
     1921 aagttctatc gaggcttata agtccctgaa acaaaaaaat gcctgaagtt caggcatttt
     1981 ttacatcatc aatatctact aaaaagctta caacaatcaa aaaacgagag ttttattctt
     2041 gtgaatacga cttaactttt tcaaaaattg gatagaaatc taatccctca tctttcatcg
     2101 tagaaatcaa cgcaacgatt acagcaatat gtgtaggaac cacaccttgt ttactgtaat
     2161 tcgtaatcga attaggtttc atatccaata aggcagcaaa ttccttaatt gaaagccctg
     2221 ctttaccgac aagacgttga aattcagtat atggcattta attaaattta tagaaatggt
     2281 gttttgagat tatagcatag ggttatattt acataaaaac acataaaatt atgtggtaaa
     2341 cacatatttt tatgtgttat aaatagctta tcaactaacc tatttatcct tactatcgga
     2401 gaaatgtaat atgtactcat acccggattc aaacactgaa aagaaaattg ctctcatgat
     2461 catcaatgac tttttcattc aaaaagcaca tgaactgtgg atttttttac aactcgatca
     2521 gtgctttaat gattatgaag ccacactgat ttggactcga cgttatttag agaagcatcc
     2581 tgagtgtgag tacagcgata ttcaaaaagc ctttgtttct tgtttcccag agcacttctt
     2641 cagctttgac tgttaattta tgagaggcac ggtaaaaaat cgcaattggt aagtaaatcc
     2701 caaatagaaa gagccagttc atacaactgg ctttttattg tcacgtataa atctaagact
     2761 tgttttaatc gttgctaaca cgatcaagca aagtgtaata acgaaactaa aagtgcagca
     2821 acagactttt tttttgcaga aataaaactg aaaatgcggt aacagaaacg tttttgtaga
     2881 aacgaaactg aaaatgcagc aacggacatc ttttttgccg gaaacaagcg caatttaagc
     2941 gaaaagcaca gcattgtttt gattttaaaa tttaagcata ttgcttaaat atcagcagct
     3001 taggttcaat acctaagctg ctgatgccca ggggttgagg ggaaggattc cctcacggac
     3061 tgtagtttct cgaagagaaa ctgttaagtg cgaattaata aaaaataaaa tttattttat
     3121 tttttattaa ttaaatccat tcgcccaata agttatgcta caaagcataa tgaaattgaa
     3181 gcgaatggat ttcttgttaa aatgcaaaac tctgatttta aaaaacgtgg acgaccagca
     3241 cttttgctca atcaactaga gttaaaggcc tatgtcgact atgtgagaaa gtataaaatt
     3301 tgggatcaag tcactcaaaa tgcagttcgt gaaattcaag catctcaatt tgaaatgcag
     3361 aacttgtcac caactgcatt agtcatcgta aaacaagcca ttaaaccatt tagagagctt
     3421 caaaaagttg aactgcttta tcaacaactt tgtaaaacta catcgcatga tttctttcaa
     3481 aaaaagttcg tcgagttata tcaacaatac caaagcactc gtgaagatca gacactgaat
     3541 gtgcttaaaa caatggcaac tcaatatctt cgttttaaag acaacaaagt cgatgaaaat
     3601 agcttaaaac tgtatcttag tcagttagag aaaaaagaga ataaagcaaa acggaatgct
     3661 gataacaaga aaaaatttga attaggagga gcactattag ccttgttaaa aacaaaaaat
     3721 attgatgtca ccaaactgac cgcagcacaa atgattcgtg ctctttttag tcaagatatg
     3781 cactttaatt tagccaattg caatacgatc atttttcaag aaatgctcaa tgttggcctc
     3841 agtgaaactc aagccaagac cttgtttcca ctggttttag atcaactcac agactatcgc
     3901 gacgaagatg gtttgccaat ttatttaaag caaattatta aaacaatggc agaaaatggc
     3961 tagctatcac ctgcaactaa gagacaaacc caaaggctat gcactcgaac attatttcta
     4021 tattgctcga ttagaaaaat atcagcatgt gcgtgtcaaa agccaagaag ttttagaaca
     4081 tattgaaccg ggtggttgta tgccatcttg ggcgaaagat cctgctgact tctggaaagc
     4141 agcagatacc catgagcgag ccaatgccaa agtctacatg gaatatgaat tggctctccc
     4201 caatgaactt acaccagaac agcgtaaaac actggtagag gcatttttaa atgaacacgt
     4261 agccagtcac caatatccgc attcttacgc catacacaac gttaaatcga gaattggcgg
     4321 cattgatcaa ccgcattgtc atctcatgtt gtctttgaaa gccaatgatg gccttgaccg
     4381 cacgccagaa acctacttca aacgctttaa tccgaaaaac ccggagaaag gcggggcgaa
     4441 aaaaagacaa cttgatgaag gctttgacaa ctaccgttca ttcttactgt atatccgcaa
     4501 ggaatgggaa aactatatga atgatgcctt acgccaatat gccccgacct tgacttacca
     4561 aatcgatgga caagagatca acatccccaa tcgagtatcg tcttcaagtt ataaacaata
     4621 taatttagat cacggcacct tttacattgc cgaacccaag cttggagttg gcaatcaaaa
     4681 caatacacct gaatatttag agcagatcaa acaaatccgc gagcataacc aacaagaatt
     4741 tgaacgagaa caactccaga aacaccagga actcaacctc agtgctaaac cgttttacag
     4801 tgtgaatgac cagccgtata gcagtttaga agtaacagcc ttattgaaga gtttaaatcc
     4861 agatacccac tcagtagaaa taaccgcgat tcaacagcaa caagctttag ttgatccatc
     4921 ctttaaaaat caagaactca atctcactcc tgaaaataaa ccaacaccga ctattaaaaa
     4981 agacactggc tttaactttt cgatgtaaaa accaagatga gcaaagcgag tgtctacgag
     5041 cgacatcagc aaaattcgcg cctagactct ctgaaaaaca gctttttagg ctgtgaagcc
     5101 taaataagcg taaatcacat gtaaaaaatt gagcctagac cgagcgtagc gagtaaaaag
     5161 gctttatgag cgaagcgaat tccgagttgc ttttgatctt gcttctgctt ttcctcaaat
     5221 tcacggaaac attaacctaa aaattgcccc gacgaatcga gcgatagcga gattcaatag
     5281 agtttgagcg tagcgaaaac caagggcaat ttttcattcc ccaggctttt aattattttt
     5341 aaggttttta aatgctttta aacacgctga aagccttttt ttgcaaggct ttcaaggtgg
     5401 ataagactac aatttctttg tcataagact acaatttctt tgtcataaga ctacaatttc
     5461 tttgtcataa gactacaatt tctttgcatt cgattacatc gaatgataat gtagtcttaa
     5521 taacacttac aacctatgca gtcttatgaa aaatgactta gtagtaaaag ataatgcctt
     5581 aattaatgcc agttataatc tcgaagtaac cgagcaacgg cttatccttt tatcaataat
     5641 aagagcaaga gaaacaggcc aagggatctc ttccgacagt aaattagaaa tccatgcttc
     5701 tgattatgct tctcgctttg atgtcaccaa agaagctgcg tataacgctc taaaaaatgc
     5761 agttaataat ctttttgaaa gaaagttctc ttttaaggaa attcataaag atacaaacaa
     5821 agaaattgta gtcaaatccc gctgggttag ccgaattgca tatgttgatg accttgctac
     5881 attagaaatt acctttgcac cagatgttgt tcctcttatt acaagattgg aaaagcactt
     5941 tacgagctat caacttaaac aggttgcgca actaactagc aaatatgcta ttcgtctata
     6001 tgagtttcta attgcttgga gagacgtagg aaaaacacct gttatttctt tatctgagtt
     6061 cagagaaaga ttaggacttg agatgaatga atatcaaaaa atggttaatt ttaaaagtcg
     6121 tgttttagaa ccagccataa agcaaatcaa tgagctgact gatatacatg taaaatacga
     6181 acagtacaaa acaggaagat ctatctcagg cttatcattc atttttaagc ataaaaaaat
     6241 tgattcgata ccttcagaaa gagatccaaa cacggtagat ctatttaccc aaatgacgga
     6301 tgcacaacgc cacatgtttg ccaataaact ttcagagcta ccggaaatgg gtcggtattc
     6361 ccagggaact gaaagctatc cacaatttgc tattcgtatt gctgagatgc ttcacgatcc
     6421 tgaaaaaatc aaagaactat atccatacct aaaaaaagtg ggatatatgc cattaaataa
     6481 aaggatttag taaatggcta aattatcact aagtgaagta tctaaccgct ttaacgtcag
     6541 tcgttctacg ctatataggg ccataaaaga agggcgtata tctcgaagct ctgatggtct
     6601 ttttgatatt gcagaagtca tccgatgctt tggagagccg atcaaaaagc atgaacaaaa
     6661 acaagaagtg gatcagtcta aagatgatgc tgatttacgt cagcttgttg attttatgaa
     6721 aaaggaaatt gagtcataca aagatcgtga gaaacgctat ttagatcaaa tagaccgctt
     6781 tcaattgcta ttagggcata aagagtctga agaaaaaata tctcatgatg caccagtgac
     6841 acattccaat agtgcaccat gcgacaacaa tagtaaaata agtgaaacta ttgataataa
     6901 tagagatagt gaaattaaaa catcccatga gacattgggg acacgcccta atgaaacacc
     6961 aaaacacgta ccaaatacgc attatgagac aaagaaaaaa cgtggtttat ttggccgtgt
     7021 actaaatgct gtttttgata atgactaagg gagagagatc atgccgaaac tgaaagacat
     7081 tgccctggga attattgtgg ccccgctgtt gatcccgatc atgctgattg catcgtacca
     7141 ggataaaaag gcactcaaaa aagagctgga tgaacgccaa aaagaaaaag accaggcatc
     7201 ctgattattc aactgagcta attaaaagtg tggaacattg cttaaataat agacgtggaa
     7261 cgtgaaaaat cataaaaaaa gcccaccttg gggaaggtgg gctataaact agaaactaca
     7321 gcattgattt ctaaggaaaa ttataacaca tttcgtataa ggtgtattat gttaatttta
     7381 ggaaaactta acccagaata ctattgcgaa ggtttaactg gaaaacctat aaatatttta
     7441 tttttttcat atatttgtga gaaaattact aaaaatttcc aataaatttt cctatgatca
     7501 ataatctaat attttcacta tcacctatcc ttctttcttt attattggga tggatcgttg
     7561 gtaagacatc tagagaagct cttagattcc gattggtaag tcttattact atcttagtat
     7621 ggcttcttct gatctcaatt ggattccagt ttggaagtgt actatttgat agaaaattag
     7681 gttctagtat cctgatagaa gcattttttt acgctactat tctttcatta attaccttta
     7741 ttcttctttt tagaaaaaat cttatttcta atgataagaa aaaatcttta gaacaaattt
     7801 ttaagccaat tttagaatgc ctaattgcta tatttatggt tgttattggg atgtcgttat
     7861 actcaatttt gcctcaaaat atcaatagtg aactaataag cagtatctta ttatatattc
     7921 taattttttt agtaggagtc gatctttctt ctataaaaat acagttctta actcttgatc
     7981 atatcaaagt accaattttc actattcttg cattatttat atcagcatat atcacaacat
     8041 tttttttaga aaaatctttt attgaattag tagttttggg aagtggattt ggttggttct
     8101 ctttatcagg cccattagtt ggaaagactt taggggtgga acaaggtacc tttgctttac
     8161 ttgtggactt agttcgagag ttttatgcaa ttggtctgtt atatctgtta ggtcgagcat
     8221 atccaaatcc aataattggg atatgtggcg caactgcgat ggattctaca ttgcctttta
     8281 tcaaaaacaa ttgttctcaa ttaaatgttc aaatagctat ttttagtggc tttatcttaa
     8341 ctttgattgc accatttttt attatcatat ttagtaattt tgtttaacat aatggcggtt
     8401 atacgaaatt agtagttttt acgcggaatt cctaacgtat tcatcttttg tcttatatct
     8461 ttatttttac caataggtaa aatatattca taacctacat cgaaaagtag gtaattaaga
     8521 aaaaaattac agcgttttat aagccctata gtaacgggag agactatagg gctttttatt
     8581 tatcttaata ttcatatatc tatgatgaag ccatgatact ctgcgatata aaaaaccggc
     8641 ttaagaatag accggtcttt tatgatattt gctaaaatac tcttttgaaa tataaaaaac
     8701 taagcgtgta tagcactggg tctaggtgtg agagcaacaa ctctcgtttc gcataattcg
     8761 tattatgtta attttgataa aactaatcaa aattagattt tttaatattt tacatattga
     8821 ctcatatgca ttacatatgc tttcatattg aaaataaata tcaagagagt acttcctatg
     8881 caacttagta ttgaaaatcc tagtctcgac
//
