LOCUS       NZ_CP080517             3383 bp    DNA     circular CON 26-JAN-2024
DEFINITION  Salmonella enterica subsp. enterica serovar Minnesota strain SA18578
            plasmid pSA18578_4, complete sequence.
ACCESSION   NZ_CP080517
VERSION     NZ_CP080517.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN20518183
            Assembly: GCF_019468645.1
KEYWORDS    RefSeq.
SOURCE      Salmonella enterica subsp. enterica serovar Minnesota
  ORGANISM  Salmonella enterica subsp. enterica serovar Minnesota
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Salmonella.
REFERENCE   1  (bases 1 to 3383)
  AUTHORS   Alzahrani,K.O., Alshdokhi,E.A., Mujallad,M.I., AL-Reshoodi,F.M.,
            Alasiri,A.S., Alswaji,A., Alzayer,M.A., Okdah,L., Alghoribi,M.F. and
            Alajel,S.M.
  TITLE     Complete genome sequence of colistin-susceptible Salmonella enterica
            serovar Minnesota strain harboring mcr-9 on an IncHI2 plasmid
            isolated from chicken meat in Riyadh, Saudi Arabia
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3383)
  AUTHORS   Alzahrani,K.O., Alshdokhi,E.A., Mujallad,M.I., AL-Reshoodi,F.M.,
            Alasiri,A.S., Alswaji,A., Alzayer,M.A., Okdah,L., Alghoribi,M.F. and
            Alajel,S.M.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-AUG-2021) Infectious Diseases Research, King Abdullah
            International Medical Research Center (KAIMRC), KAIMRC, P.O Box
            22490, Riyadh 11426, Saudi Arabia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: EToKi pipeline v. 1.0
            Assembly Name                     :: SA18578
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 40.0x
            Sequencing Technology             :: Illumina MiSeq; Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/24/2024 14:22:26
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,268
            CDSs (total)                      :: 5,151
            Genes (coding)                    :: 4,980
            CDSs (with protein)               :: 4,980
            Genes (RNA)                       :: 117
            rRNAs                             :: 7, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 13
            Pseudo Genes (total)              :: 171
            CDSs (without protein)            :: 171
            Pseudo Genes (ambiguous residues) :: 0 of 171
            Pseudo Genes (frameshifted)       :: 72 of 171
            Pseudo Genes (incomplete)         :: 123 of 171
            Pseudo Genes (internal stop)      :: 26 of 171
            Pseudo Genes (multiple problems)  :: 45 of 171
            Pseudo Genes (short protein)      :: 1 of 171
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP080517.1.
            Bacteria and source DNA available from Sulaiman Alajel.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3383
                     /organism="Salmonella enterica subsp. enterica serovar
                     Minnesota"
                     /mol_type="genomic DNA"
                     /strain="SA18578"
                     /serovar="Minnesota"
                     /isolation_source="food"
                     /sub_species="enterica"
                     /db_xref="taxon:70803"
                     /plasmid="pSA18578_4"
                     /geo_loc_name="Saudi Arabia"
                     /lat_lon="24.774265 N 46.738586 E"
                     /collection_date="2020-07"
                     /collected_by="Sulaiman Alajel"
                     /genotype="ST548"
     gene            complement(join(3318..3383,1..261))
                     /locus_tag="K1T32_RS26105"
                     /old_locus_tag="K1T32_26105"
     CDS             complement(join(3318..3383,1..261))
                     /locus_tag="K1T32_RS26105"
                     /old_locus_tag="K1T32_26105"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_220234462.1"
                     /translation="MIVKFHARGKGGGSGPVDYLLGRERNREGATVLRGNPEEVRELID
                     STPFSKKYTSGVLSFAEKELPPGERERVMTSFERVLMPGLEKVSKKTSTAFSGWNTRIR
                     GGSN"
                     /name="hypothetical protein"
     gene            complement(251..574)
                     /locus_tag="K1T32_RS26110"
                     /old_locus_tag="K1T32_26110"
     CDS             complement(251..574)
                     /locus_tag="K1T32_RS26110"
                     /old_locus_tag="K1T32_26110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_024132211.1"
                     /translation="MLTIRVTDEEHARLLERCEGKRLAEWMRRVCLGEPVARTGKLPTL
                     SPPLLRHLAAIGNNLNQTARKVNSGQWSSIDRVHVVAALMAIEGELRQLRQAVREQGVR
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            638..829
                     /locus_tag="K1T32_RS26115"
                     /old_locus_tag="K1T32_26115"
     CDS             638..829
                     /locus_tag="K1T32_RS26115"
                     /old_locus_tag="K1T32_26115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165979.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNDLDADEQADICESLHDHA
                     DELYRSCLARFGDDGESN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(2145..2378)
                     /locus_tag="K1T32_RS26120"
                     /old_locus_tag="K1T32_26120"
     CDS             complement(2145..2378)
                     /locus_tag="K1T32_RS26120"
                     /old_locus_tag="K1T32_26120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137389.1"
                     /translation="MTELEGHLLNALEHLQQDYMRRLNEWESAFAELQKMHEVTQRNNA
                     ILNERVVILSQQVQRLAGQVDRLSRLFIANNS"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(2385..2945)
                     /locus_tag="K1T32_RS26125"
                     /old_locus_tag="K1T32_26125"
     CDS             complement(2385..2945)
                     /locus_tag="K1T32_RS26125"
                     /old_locus_tag="K1T32_26125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_032329292.1"
                     /translation="MSNLLQMGTDFEKKLKERAASTENMLNSEFRKLEESVDKALSLNR
                     QKIRDAISGHTTSVKQQLDTLSATVSTQFSTTEAELSRQQKKLLWQVIKGRILFPALTA
                     LSVTGGIFLGCWGLMEWQESKIAKNILTIREQENTLAKLEAKTWGVTFVNGENGKFLVL
                     PDGVKGENTWTVGDKNAVRLVRE"
                     /name="MbeB family mobilization protein"
     gene            complement(<2977..3351)
                     /locus_tag="K1T32_RS26130"
                     /old_locus_tag="K1T32_26130"
                     /pseudo=""
     CDS             complement(<2977..3351)
                     /locus_tag="K1T32_RS26130"
                     /old_locus_tag="K1T32_26130"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     Typing          complement(911..991)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="87.805"
                     /coverage="93.0"
                     /evalue="8.68e-18"
     Typing          complement(255..607)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="84.746"
                     /coverage="100.0"
                     /evalue="9.45e-59"
     Typing          complement(2063..3381)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="91.91"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 tttttcgaga ccgggcataa gaacacgctc aaagctcgtc atcactcttt cgcgttcacc
       61 cggcggtaac tccttctccg cgaacgacag aacgcctgac gtgtatttct tcgaaaatgg
      121 cgtggaatca atgagttccc gcacttcttc cgggttaccc cggagcaccg ttgcgccttc
      181 gcggttacgc tcccgcccca gcaggtaatc gaccgggcca cttccaccgc ctttccccct
      241 ggcatgaaat ttaactatca tcccgtaccc cctgttccct gacagcctgc cgcaactggc
      301 gcaattcacc ctcgatggcc atcagtgcgg ccaccacatg aacccggtca atcgaagacc
      361 actggccgct gttcaccttc cgggctgtct ggttcaggtt gttcccgatg gcagccagat
      421 gccgcagcaa tggcggcgat agtgtcggca gttttcctgt gcgtgcaacc ggctccccca
      481 gacagacccg ccgcatccat tccgccagtc gtttaccctc gcagcgttcc agtaaccggg
      541 cgtgctcctc atcggtaacc cgtatcgtga gcatcttctc gcgtttcatc ggtatcattc
      601 cccctaacaa atcccccata caaggagcct taagaccatg accaaacagg aaaaaacagc
      661 ccttaacatg gctcgcttta tcagaagcca gacgctaacc ctgctggaaa aactcaacga
      721 tctggacgcg gacgaacagg cagacatctg cgaatcactt cacgaccacg ccgacgaact
      781 ctaccgcagt tgcctcgcac gcttcggcga tgacggtgaa agcaactgaa acatgcagct
      841 cccggagacg ttcacagctt gcctgtgagc ggatgccggg agcagacaag cccgccaggg
      901 cgcgtcagtg cgggtgtcgg ggcgcagccc tgacccagtt cacagagcga tagcgatgtg
      961 tatactggct tatctcattt tgcatcggtg cagacatgga tgggaagtgc accatgtcag
     1021 gagtaaaaag ccgcacagat gtaaaaacag cgcacccgat aaacagcaca cacgcggaca
     1081 ctggcacagg cgcgtaagtg cacaatgctt gcatctgtga ctcttccgct tcctcgccca
     1141 ctcgctcact acgttcggtc gctggggctg cggcgagcgg tgggatcact ctcgttgtga
     1201 tgcagatccg tttttttttt tgcactggag ttatcaggag ttgcggtgca catgggatcg
     1261 tgcgatatgc cggatggtga aaggttggag ggaacggcaa caactcaccc ctcccagtga
     1321 agggacgggt agtttgccgc tctcgatatg ccggacggag ccaaggccat cgatcacggg
     1381 gcgcgtcagt ggaagaaccc gcgcctgcgt gacgtatgcg tcttcgccgc accatctcac
     1441 ccgtcacgcc accacatacc gggaactgcc gccaaggtct aactcaacca atctagccgc
     1501 gctacatacg gtttttgtgg caactcatca ggtgtccgct gtcgatgaaa ctctctgcgc
     1561 cggtagcgga actctcacgc gtgcggtcgc cgtcgtcaca gctcccccaa ccgcaaacga
     1621 ttctcgcaaa tacgcaagta tctgcaaaaa caggtttttt tcggggttcc tgggagactg
     1681 tgctgttgaa aaattcttga agaatacaga aggctcatgc aaactgaatg aactggtttc
     1741 tgttgtggat ctcaacagtt tccacaggaa cattgaggag gtcagagacc tcaatgaacc
     1801 cgaaacagct cgccttcctg gcgagctgtt ttcgttctac gcccgccgac gaaagatcgc
     1861 aagtgcaata acgagatttt tcacgtcttt acgctcaaaa agtaaaaaaa ccatgttttc
     1921 ccctggctga atccccaaaa acgcgtgaaa tgcgttttca ggatgttgca tagctatgca
     1981 caaatacggc acacgatttg tttcgctcgc cccagccgtc gaactggtca aaatggcaag
     2041 atttcgtcac ttccccggtc acatctccat tgatggccca ctccgaaccc gtgccgcgcg
     2101 ttcctgtacc tcatgtctgt accgggcacg ctcctgttca tcccttagct gttgttcgcg
     2161 ataaataacc gactcaagcg atccacttgc ccggctaatc tctgcacctg ctgactcaat
     2221 atcacgacgc gctcgttcag tatcgcgttg ttccgttgcg taacctcgtg catcttctgc
     2281 aattccgcga aggcgctctc ccactcgttc agccgccgca tatagtcctg ttgcaaatgc
     2341 tctaaggcgt tcagcaaatg cccttccagt tccgtcatac tcgtttactc cctgaccagc
     2401 ctcacggcgt tcttgtcgcc caccgtccag gtgttttccc ctttcacccc gtccggcaac
     2461 accagaaatt tcccgttctc gccattgacg aacgtcaccc cccaggtttt tgcctcgagt
     2521 ttcgccagcg tgttttcctg ctctctgata gtcaggatat ttttcgcaat cttgctttct
     2581 tgccactcca tcagccccca gcaccccagg aaaatgccgc ccgtgacgct gagcgcggtc
     2641 agcgccggaa acagtatccg ccctttgatc acctgccaga gcagtttttt ctgctgccgg
     2701 gacagttcgg cctctgtggt gctgaactgc gtgctcacgg tggcgctcag tgtatccagt
     2761 tgctgtttca cggatgttgt atgtccgctg atagcgtctc tgattttctg cctgtttaag
     2821 ctcagtgcct tgtctacaga ctcttcgagc ttcctgaact cgctgttcag catgttctcg
     2881 gtagaagcgg ctcgctcttt cagtttcttc tcgaagtctg ttcccatttg taaaagattg
     2941 ctcatacagc gcccccttta gcctgagatt ccgcccaccc tccgggtcgg caatactgat
     3001 actgcttctg gttgtcctca ccacctcaaa acctgccgct gtgagcgcct cagtgacatc
     3061 ctgacgcgtt tttattgctc cggcatggta aagcgcctca agaccccgtg taatcgcttc
     3121 tgcggcctcc tgtttcgctt ttggcagatt atttggcgtg acaagcgtcc gtctgttctc
     3181 cggtgcgttc gggtcacgca gcccgtaatg atgattaacc agcgtctgcc aggcatttat
     3241 cctcggcctg tccgcacggt cgtagtacgg ctggaggcgc ttaccgctct gtagctccat
     3301 gttcgggatg acaaaattca gttcgagccg ccccttatcc tggtgttcca cccagagaat
     3361 gctgtactgg tctttttcga gac
//
