LOCUS       NZ_CP080475             4935 bp    DNA     circular CON 15-APR-2024
DEFINITION  Enterobacter hormaechei strain ECC59 plasmid pECC59-5, complete
            sequence.
ACCESSION   NZ_CP080475
VERSION     NZ_CP080475.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN20500401
            Assembly: GCF_019458525.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter hormaechei
  ORGANISM  Enterobacter hormaechei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 4935)
  AUTHORS   Zhou,K.
  TITLE     Carbapenem- and colistin-resistant Enterobacter hormaechei
            co-producing NDM-1 and MCR-9
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4935)
  AUTHORS   Zhou,K.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JUL-2021) Institute of Respiratory Diseases, Shenzhen
            People's Hospital, Dongmenbeilu, Shenzhen 518020, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 110.0x
            Sequencing Technology             :: Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_019458525.1-RS_2024_04_14
            Annotation Date                   :: 04/14/2024 21:40:12
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,080
            CDSs (total)                      :: 4,966
            Genes (coding)                    :: 4,809
            CDSs (with protein)               :: 4,809
            Genes (RNA)                       :: 114
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 57 of 157
            Pseudo Genes (incomplete)         :: 90 of 157
            Pseudo Genes (internal stop)      :: 30 of 157
            Pseudo Genes (multiple problems)  :: 18 of 157
            Pseudo Genes (short protein)      :: 1 of 157
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP080475.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4935
                     /organism="Enterobacter hormaechei"
                     /mol_type="genomic DNA"
                     /strain="ECC59"
                     /isolation_source="Broncho-alveolar lavage"
                     /host="Homo sapiens"
                     /db_xref="taxon:158836"
                     /plasmid="pECC59-5"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="22.38 N 114.05 E"
                     /collection_date="2017"
                     /collected_by="Shenzhen People's Hospital"
     gene            join(3992..4935,1..361)
                     /locus_tag="K0823_RS25265"
                     /old_locus_tag="K0823_25265"
     CDS             join(3992..4935,1..361)
                     /locus_tag="K0823_RS25265"
                     /old_locus_tag="K0823_25265"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /GO_process="GO:0006935 - chemotaxis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing methyl-accepting chemotaxis
                     protein"
                     /protein_id="WP_033488194.1"
                     /translation="MFSLKYMFSLNLQRSAISYDAICQNKPVIEFSPEGVINKASPLFL
                     STMGYRADEIIGHHHRIFCPPSLVSSPEYSQFWQRLARGESFSGKYLRLAKGNRSVWLE
                     ASYIPVSDRRGRVIRVIKIAADISERVHSALEQEAVVNAINRSMAIITFNPEGIVLEAN
                     ENFVNATGYKRDEIIGKHHRLFCAETLYKSDEYRHFWERLNQGEFFSGLFPRLNRQGDP
                     LWFRATYNPVFNSDGQLYKIVKFATDVTADVLRNQREQEAAVHAWDMAVQTRESAQNGA
                     NVIENSILMIDRIAQGMGAVSTDISRLNNQSESIDDMVETIRKFAMQTRLIALNAAIEA
                     ARAGASGRSFAVVAAEVRNLAASVSSATEEIEQVVASNSQLAKDVLCGIENSLMNTRKG
                     VTLMREAGEVMTSIQRNAAGVETAVKDVAISVKAG"
                     /name="PAS domain-containing methyl-accepting chemotaxis
                     protein"
     gene            1368..1691
                     /locus_tag="K0823_RS25270"
                     /old_locus_tag="K0823_25270"
     CDS             1368..1691
                     /locus_tag="K0823_RS25270"
                     /old_locus_tag="K0823_25270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_176490892.1"
                     /translation="MLTMWVTEDEHRRLLERCDGRPLAAWMRQTCLDEKSARTGKLPSI
                     SPALLRQLAGMGNNLNQIARRVNAGGGTGHDRVQIVAALMAIDAGLERLRHAVLEKGTD
                     DDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            1900..>2334
                     /locus_tag="K0823_RS25540"
                     /pseudo=""
     CDS             1900..>2334
                     /locus_tag="K0823_RS25540"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            2369..2860
                     /locus_tag="K0823_RS25280"
                     /old_locus_tag="K0823_25280"
     CDS             2369..2860
                     /locus_tag="K0823_RS25280"
                     /old_locus_tag="K0823_25280"
                     /inference="COORDINATES: protein motif:HMM:NF016710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_047720453.1"
                     /translation="MSSLLTLAKDLEQQSKAQQKSTGEMLKAAFSEHEQSVRAELNASA
                     RRISDAISAHEQSMSEAMEKNRRSVLLTAGRTWLTILMVSALLIATSGSILWWQGQQIT
                     DNYTHLRQQEDTLAKMTARTWGVRYQESSDGRRFLILPPGMQAEAIPYDGTTWIRLKQE
                     "
                     /name="MbeB family mobilization protein"
     gene            2867..3082
                     /locus_tag="K0823_RS25285"
                     /old_locus_tag="K0823_25285"
     CDS             2867..3082
                     /locus_tag="K0823_RS25285"
                     /old_locus_tag="K0823_25285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137422.1"
                     /translation="MTELEKQLLSALEQLQQDYSQRLDEWESAFAEWRSMCGLMQRENA
                     VLNERVSDLSTQVLSLSEQLRRLSGN"
                     /name="MbeD family mobilization/exclusion protein"
     Typing          1083..1255
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="98.276"
                     /coverage="100.0"
                     /evalue="9.21e-81"
     Typing          complement(612..1155)
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.698"
                     /coverage="90.0"
                     /evalue="0.0"
     Typing          1682..3180
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="90.297"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 tgacgatatg gtggaaacca tccggaagtt tgccatgcag acaagactta ttgcactgaa
       61 tgccgcgatt gaagcagcaa gggctggcgc atccggaaga agttttgcgg ttgttgccgc
      121 agaagtgcgt aatcttgcgg cgagcgtcag tagcgcgact gaagaaattg agcaagtcgt
      181 ggccagcaat agtcaactgg ccaaggatgt tctctgtggc attgaaaaca gcctgatgaa
      241 cactcggaaa ggggttacac tcatgcgcga agcgggtgaa gttatgacca gcattcagag
      301 gaatgctgca ggggttgaga ctgcggttaa ggatgtcgcc atttcagtta aagccggata
      361 ggccatggtg catatcccca tatctgtcga acatcacaga tgatcttctt gagatccttt
      421 ccgttgcgtc gtaatctctt gctctgaaaa cgaaaaaacc acccggcaag gtggcttttt
      481 cgaaggttcg caagagttga cgctcttttg aaccgcggta actggcttgg aggagcacag
      541 tcaccaaaac tgtcctttca gtgtagcctt atcgcacccg aaacttcaag actctcctcc
      601 tctaaacctg ttaccagtgg ctgctgccag tggcgctttg tcgtgttttt ccggattgga
      661 ctcaagttga tagttaccgg ataaggcgca gcggtcggac tgaacggggg gttcgtgcat
      721 acagtccagc ttggagcgaa ctgccttccc ggaactgagt gtcaggcgtg gaatgagaca
      781 aacgcggcca taacagcgga atgacaccgg taaaccgaaa ggcaggaaca ggagagcgca
      841 cgagggagcc accaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
      901 actgatttga gcgtcagatt tcgtgatgct tgtcaggggg gcggagccta tggaaaaacg
      961 gcttctccgt ggccttctcg cttccctgtt aagcttcttt ctggcatctc ccaggaaatt
     1021 tccgccccgc ctgtaagccg gtaccgctcg ccgcagccga acgaccgagc gcagcgagtc
     1081 agtgagcgag gaagcggaat atatcctgta tcacatattc tgctgacgct ccgctgctgc
     1141 atttttctcc tgccagatga agcacttcac tgatattcgc atccgtgcca atatagtaag
     1201 ccagtataca ctccgctagc gctacgtgac tggttcaggg ctgcgccccg aaacccgcca
     1261 aaagcctgac gcgcctgcgg cttgtccaac cccgcgccga ccgggaaagg ctttcccgcc
     1321 cgtcccggcg ttatcaggag gccggtttgt cagagaaacg gaacaagatg ctcaccatgt
     1381 gggtgacgga ggacgagcac cggcggctgc ttgagcgctg tgacggcagg ccgctggcgg
     1441 cgtggatgcg gcagacctgc ctggacgaaa agtctgcgcg caccggaaag ctcccctcaa
     1501 tctcgccggc gctgctgcgc cagcttgccg gcatggggaa taacctgaac cagatagccc
     1561 gccgtgttaa cgccggcggc ggcaccggcc atgacagggt gcagattgtg gcggcgctga
     1621 tggccatcga tgccgggctt gagcggctgc ggcacgccgt tcttgagaaa ggaacggacg
     1681 atgatcgtta aatttcatcc acgtggccgc ggcggtggcg ccgggccggt ggactatctg
     1741 ctgggtaaag accggcagcg tgaaggcgcc agcgtcctgc aggggaagcc ggaagaagtc
     1801 cgggagctga ttgacgcgtc gccttacgtg aagaaataca cctccggcgt cctgtcgttt
     1861 gcggaggccg atctgcctcc cggccagcgg gaaaaactga tggccagctt cgagcgggtt
     1921 ctgatgcccg gacttgataa agatcagtac agcattctgt gggtggagca cgaggacaag
     1981 gggcgtctgg agctgaactt tctgatcccc aataccgagc tgctgaccgg caggcggctc
     2041 cagccgtact acgaccgcgc cgaccggccg cgtatcgatg cctggcagac catcgtgaac
     2101 ggcagactgg ggctgcacga tccgaacgcg ccggaaaacc gccgggcgct ggtcacgccg
     2161 gccggactgc cgaaaacgaa gcaggaagcc gcagaagcca tcacccgtgg actgctgagc
     2221 ttagcctcgt ccggggagct gaaaagccgt caggacgtca ctgaggctct tgaaggtgca
     2281 ggttttgagg tggtgcgtac aacgaaaagc agtatcagca ttgccgaccc tgacgggggg
     2341 cgaaacatcc gactgaaggg agccatctat gagcagtctt ttaacgctgg cgaaggactt
     2401 agagcagcaa tcgaaagcgc agcagaagag taccggcgag atgctgaaag ccgcattcag
     2461 cgagcacgag cagtctgtca gagcggaact gaacgcaagc gcgaggagaa tcagcgacgc
     2521 catcagcgcc cacgagcaga gtatgagcga ggcgatggag aaaaaccggc ggagcgtgtt
     2581 gctgacggcc gggaggacat ggctcaccat cctgatggtg tcagcgctgc tgatcgccac
     2641 gagcgggagc attctgtggt ggcaggggca gcagataacc gacaattaca cgcatcttcg
     2701 tcagcaggaa gataccctgg cgaaaatgac cgcccggacg tggggagtgc gctatcagga
     2761 gagcagcgac ggccggaggt ttcttatcct gccgccgggg atgcaagccg aagccatccc
     2821 ctacgacggg acgacatgga tccgcctgaa acaggagtag cggaaaatga cagagctgga
     2881 aaagcagttg ctgagcgcat tagagcagct acagcaggac tactcgcaaa ggctggacga
     2941 atgggagagc gccttcgcgg aatggcggag catgtgtggt cttatgcaac gggagaacgc
     3001 ggtgctgaac gagcgcgtgt cggacttgag cacgcaggtg ctgagtttaa gcgagcagct
     3061 gcgccgcttg tccgggaact gaatgccatc gaggagaagg ctgcactgga gtatcagcgt
     3121 cagaacgagc tggagctgac gctccgacag aagcagaaga gtttcgatgg tccgaagatg
     3181 aggtgacagc ccgcacgaag aagggctcat cgcgttcctg ctaaataggc tgactaccgg
     3241 aatggtagtg acttatcccg gaggaagtga tgaaaattct caacgttatt gaagccctgc
     3301 tgaactgttt ttacctggtg atggagctgt gtgagttgcc ggagaggcat ttgccggttt
     3361 ttaaggcgtt cgcgctgatt ttagtgtgac tttacgctgc atgcatctgg ctgcatgact
     3421 atgcatcagt gtaatatcca gcggctgcgt ttttggtgtt ttttcgtcat gtctgtcgcc
     3481 actttctcgc gtctggcacc ggaaacgcag ccaaccctgt ttctgcggtg cgggcaaggg
     3541 gtgttatgtt aaaagccgag gtctggcgga tacggaaaaa attatgccgg ccgggcttat
     3601 gcccgcggaa atccggccga tacttaaatt atccttcaca aaggatatcc tgcgatccag
     3661 tcagttgagt gttttccgtc tcccggatgg gagccatttt tttgccttct gatgcgtgtt
     3721 aaggcttgcg gagctgcgct ggttcttcca agattgagag acgccagctt cctgctggag
     3781 ccggcggatt tgccgtaaat atgaacattt tcttattgtt cagcccgcta aagatggcgg
     3841 gttttttacg cgtgaaatcc ggagccggac atcatgccgg catggctgaa gagcgggctt
     3901 tttcccgccc tgtcgcagta atatataaag ctacataagg agtagccgat aaccatggca
     3961 gtacttacat tatccgggca atctgattca tatgttttca cttaaatata tgttctccct
     4021 gaacctccag cgatcagcca tttcgtatga cgcaatttgt cagaataagc ctgtcattga
     4081 gttcagtccg gagggggtga taaataaagc cagcccgctt tttctctcca ctatggggta
     4141 cagggctgat gaaataatcg gtcaccacca tcgtatattc tgcccgccct cgctggtaag
     4201 ttcaccggaa tacagccagt tctggcagcg ccttgccagg ggagaaagct ttagtggcaa
     4261 gtacctgcgg ctggcaaagg gaaatcgttc cgtatggctt gaagccagtt atatccccgt
     4321 ttctgacagg cgcggccgtg ttatcagagt cattaaaatt gctgccgata tttctgagcg
     4381 cgtgcattct gctcttgagc aggaagcagt cgtaaatgcc ataaaccgtt ctatggccat
     4441 catcaccttc aatcctgaag ggatcgtgct tgaagcaaat gaaaatttcg tcaatgccac
     4501 tggctataag cgggatgaaa tcataggtaa gcatcatcgt ctgttctgtg ccgagacgct
     4561 ttacaaaagt gatgaatacc gacatttctg ggagagactg aatcaaggtg agtttttttc
     4621 tggtctgttt ccacgcctta accgtcaggg agaccctctg tggttccgtg caacttataa
     4681 ccctgtcttt aacagtgatg ggcagcttta taaaattgtg aaattcgcta cagatgttac
     4741 ggctgatgtc ctgcgaaacc agagagagca agaggctgct gtgcatgcat gggatatggc
     4801 cgtgcagacc cgagaaagtg cgcagaacgg tgccaatgtt attgaaaata gcatccttat
     4861 gattgacaga attgcgcagg ggatgggggc tgtctccacc gatatctcac ggctcaacaa
     4921 tcagtctgaa agtat
//
