LOCUS       NZ_CP080368             4687 bp    DNA     circular CON 10-JAN-2024
DEFINITION  Klebsiella pneumoniae strain K191773 plasmid pCol440I-191773,
            complete sequence.
ACCESSION   NZ_CP080368
VERSION     NZ_CP080368.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN20422820
            Assembly: GCF_019443585.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 4687)
  AUTHORS   Zhang,B. and Zhou,Y.
  TITLE     blaNDM-4-harboring K. pneumoniae in China
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4687)
  AUTHORS   Zhang,B. and Zhou,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2021) foundation medicine, Southwest medical
            university, Longmatan District, Luzhou, Sichuan 646607, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: SOAPdenovo v. 2.04; unicycler v. 0.4.7
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 100.0x
            Sequencing Technology             :: Illumina HiSeq; PacBio RSII
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/10/2024 03:33:31
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,870
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,590
            CDSs (with protein)               :: 5,590
            Genes (RNA)                       :: 131
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 93
            ncRNAs                            :: 13
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 75 of 149
            Pseudo Genes (incomplete)         :: 73 of 149
            Pseudo Genes (internal stop)      :: 13 of 149
            Pseudo Genes (multiple problems)  :: 11 of 149
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP080368.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4687
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="K191773"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pCol440I-191773"
                     /geo_loc_name="China:wuxi"
                     /collection_date="2019-12-03"
     gene            join(3139..4687,1..179)
                     /locus_tag="K0P53_RS29195"
                     /old_locus_tag="K0P53_29195"
     CDS             join(3139..4687,1..179)
                     /locus_tag="K0P53_RS29195"
                     /old_locus_tag="K0P53_29195"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /GO_function="GO:0019239 - deaminase activity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-phage dCTP deaminase"
                     /protein_id="WP_227522207.1"
                     /translation="MSLARKLREKRSNPIHSANEDSSGGDSINQITSRQSMDLVIGFCG
                     AVGSGIKSIVDVTEEELDDLGYDVYRIRVSELMQKLFSEWRDDIVTPFDRYHKLQDFGD
                     ALRNKYYPHILSEAVIHEIKIEKGIDTSSKKNKKAFLVDQLKHQDEIELLRMVYQHNFY
                     LLGVIRSEVERKRNLRDEGLSKSEVDIVIHHDRKSESDYGQQTAKAILDSDYFVKNNQG
                     QRAHLKAKVKRFIGLIHGLNGLTPTTYEKGMYAAHSASLQSACLSRQVGAAILDVNGNL
                     ISVGRNDVPKFNGGLYSADDGVNDHRCVYKGGKCYNEVRKLKIKSKISQILMSESACEL
                     GVKLTEDQAEQLADVIYKKTPVSSIIEYSRSIHAEMDAITSLARLGNGGFSDKVLYTTT
                     FPCHNCARHIVAVGITKVVYIEPYEKSLALELHDDAITEVNEHGKVIFESFEGVSPRRY
                     QKFFFSTDERKDSFGNAEKYSTKYKNHIDIQFIDSYLDFENRVVDIFRTNTNIDSGNHV
                     GRTDSTINLSSQPVPPVPPVPPVPPVPPVPPVPPVPPVPPVPPVPPVPPVPPVPPDSK"
                     /name="anti-phage dCTP deaminase"
     gene            complement(383..496)
                     /locus_tag="K0P53_RS29640"
     CDS             complement(383..496)
                     /locus_tag="K0P53_RS29640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_080882241.1"
                     /translation="MHEPPVQSDCCVLSGNYHLESNPVRHAKAPLAAATGN"
                     /name="replication initiation protein"
     gene            912..1103
                     /locus_tag="K0P53_RS29175"
                     /old_locus_tag="K0P53_29175"
     CDS             912..1103
                     /locus_tag="K0P53_RS29175"
                     /old_locus_tag="K0P53_29175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_023302508.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELDLDVEADLCEKLHDDA
                     EHLFRTLSSRLDELQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            1103..1504
                     /locus_tag="K0P53_RS29180"
                     /old_locus_tag="K0P53_29180"
     CDS             1103..1504
                     /locus_tag="K0P53_RS29180"
                     /old_locus_tag="K0P53_29180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310048.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_009310048.1"
                     /translation="MARVSISEAARLTGKSRTTLHRLIKAGDLSTCSGERNAKMVDTSE
                     LLRVFGPFEQPKGEHHSGQVSEQRDTASADQSEQVIRHLKQEIEHLNTLLLSKDSHIDS
                     LKQAMLLLENHQAKLTESPAPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(2347..2556)
                     /locus_tag="K0P53_RS29185"
                     /old_locus_tag="K0P53_29185"
     CDS             complement(2347..2556)
                     /locus_tag="K0P53_RS29185"
                     /old_locus_tag="K0P53_29185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgH/BhsA/McbA-like domain containing protein"
                     /protein_id="WP_009310047.1"
                     /translation="MKSIKTFVAVAALSMMSFGAFAQSVSATASTLDRAEAKIAAQAAE
                     QGASYKITSAQFNNRVHMTAELTK"
                     /name="YdgH/BhsA/McbA-like domain containing protein"
     gene            2944..3126
                     /locus_tag="K0P53_RS29190"
                     /old_locus_tag="K0P53_29190"
     CDS             2944..3126
                     /locus_tag="K0P53_RS29190"
                     /old_locus_tag="K0P53_29190"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_080882243.1"
                     /translation="MGKELSLNKIYDKNSRYINDSRGHELGKEKKVFDFEVEMIKKRRE
                     DAIRKIIEDSKSLKW"
                     /name="hypothetical protein"
     Typing          complement(384..791)
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.355"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 tcgggagaac tgattcgact attaatttat cttcccagcc cgtaccgccc gtaccgcccg
       61 taccgcccgt accgcccgta ccgcccgtac cgcccgtacc gccagtaccg ccagtaccac
      121 cagtaccacc agtaccacca gtaccaccag taccaccagt accaccagac tcgaaatgaa
      181 gatctgttga ctctgatgtt atacagagct taaccttgag acgagaaaga aaaaaaccgc
      241 ctttgcgggg cggttttttt aaggttctag agtttgcggc tctatgaacc aaggtaactg
      301 gcttagagga gcgcagtcac caaaacttgt tctttcagtt tagccttaac tggcgcataa
      361 cttcaagact aactcctcta aatcagttac cggtggctgc tgccagtggt gcttttgcat
      421 gtcttaccgg gttggactca agatgatagt taccggataa gacgcagcag tcggactgaa
      481 cggggggttc gtgcatacag tccagcttgg agcgaactgc ctacccggaa ctgagtgtca
      541 ggcgtggaat gagacaaacg cggccataac agcggaatga caccggtaaa ccgaatggca
      601 ggaacaggag agcgcacgag ggagccatca gggggaaacg cctggtatct ttatagtcct
      661 gtcgggtttc gccgccactg atttgagcgt cagattccgt gatgctcgtc aggggggcgg
      721 agcctatgga aaaacggccg ctgtgcggcc tttccttttc cccatcacga gaccgctcgg
      781 ttttctcgag cttttacacg catgctcctg ccgctcgccg cagccgaacg gcaggagcgt
      841 agcgactgag tgagcgagga agcggaaaag agactgttct tacactgagc actgacgctc
      901 tgaggcttct tatgacgaaa caacagaaaa cagcgctgaa catggctaaa ttcatccaga
      961 atcagtctct gctcctgctg gagaagctta acgagcttga tctcgatgtt gaggccgacc
     1021 tttgtgagaa actccacgat gatgcggagc atcttttccg tacactgtcc tcgagactgg
     1081 atgaattaca ggatggtaac tgatggccag agtgtctatt agtgaggctg cccgactgac
     1141 gggtaagagt agaacaacac tgcacaggtt gattaaagca ggtgacctgt ccacctgtag
     1201 cggtgaacgc aatgcaaaaa tggtagatac ttcagagctt ttacgtgtct ttggtccctt
     1261 tgagcaaccg aagggtgaac accattctgg acaggtctct gaacagcgtg atacagcctc
     1321 tgctgaccag agtgaacagg tgattcgtca tctgaaacag gagattgagc acctgaatac
     1381 cttacttttg tccaaggatt cgcacattga cagcctgaaa caggccatgc tccttctcga
     1441 aaatcaccag gcaaaattaa ctgaatcccc ggcgccatgg tggaaattct ggaaaaaatc
     1501 ctgaccccgt catgcttcag ttttcctctc cggccatccc ggggagttcc tgaaccttcc
     1561 caaaaatcgt cagatctctg aacgctcctc accactaaaa atacccacac cccacgcaaa
     1621 attaatattt tgcagatttg tatttaatat cagtgggtta ctttcctgaa atttcgtttt
     1681 ttaaatttta tttatataga tccgactcta tgacgactcg tcatagagtc cccggcgagt
     1741 cgtcatagag tcggtaacga ctcaggacta taaaaacaat aagttaataa cgacccgact
     1801 ctagacacga ctccccgttt ggttgttttt tagacgcggc gactctgggt acgactctag
     1861 tgaagagtcg ccggtgaatc gtgtggtgag tcgtccggcg acttgccata acgtgattgc
     1921 cggccgtcga cgaacccgat tcctgaacgt gctgaaaacc ggttttccgg ctgcgccggc
     1981 aacgcttcac ggccacgcaa taaattgcgt ccccgttcac cgttttcgga tggaaagaac
     2041 gccggaaaca ggacttactc tggctgaatg gcgtgaattt tcgcgctatg cacttgcgtg
     2101 catactcatg catgccgtaa aaacagagcc tgcgcgtttc tggcgggttt tcgggtggtt
     2161 tgttgcctgt tttaccggtt ttccgtcaga aacgccctga ggccgtttta gcggtgcgta
     2221 caatcatgcc tttatgttaa ataacagatg caaaaaagcc ccagaagggg cctgaaggtt
     2281 ttcctgctaa gggacgaaac gggccgcctc ccggcggcct gttcggcagc tccctttgcg
     2341 accatcttat ttggtcagtt cagccgtcat gtggacacgg ttattgaact gagcgctggt
     2401 gattttgtat gacgcacctt gttcagcggc ctgtgcggca atttttgctt cagcacgatc
     2461 cagggtggag gcggtagcgc tgacgctctg agcaaaagca ccgaaagaca tcatggatag
     2521 agcggcaact gcaacgaaag ttttgatgga tttcatggtc gtattcctta agtgattttg
     2581 ttctgtatgg ggcgttgttg ccctgatgtg ataaatgata ggacgaccat tagttgatta
     2641 aaatcgaaac aatttgcgga tatcattcaa aaaaaaagaa caagaattga gcgcatcaag
     2701 accagaggtt tatctctgga ttttaggtac caaatgaggt ggtggataga aatgttaaca
     2761 ttgatgattt tattttaaca aattgaaaaa taatggttag tttggcgtaa tctctcttgc
     2821 ttttctcttg ccgaatttcc taatgtttag gataagaaaa aaaaatctct atcgattacg
     2881 tttcttggct attgcctgtt ataaagataa cagtaaaatc catgcgtctt gcaggagggt
     2941 gtgatgggta aagagctatc tctaaataaa atttatgata agaattctag atatattaat
     3001 gattctagag ggcatgagct gggaaaggaa aagaaagttt tcgattttga agttgaaatg
     3061 attaaaaaaa gaagagagga cgctataagg aagataattg aggattcaaa atcattaaaa
     3121 tggtaagtga ggtcttttat gagcctggct agaaagttaa gagaaaaaag aagtaatccg
     3181 attcatagtg ctaacgagga ctcctctgga ggggatagca taaaccaaat aacctccaga
     3241 cagtctatgg atcttgttat aggattttgc ggtgctgttg gctcaggaat aaagtcaatt
     3301 gttgatgtga ctgaagaaga actagatgat ctaggatatg atgtttatcg cataagagtt
     3361 agcgagctta tgcaaaaact tttttcagaa tggcgtgatg atattgttac tccgtttgat
     3421 agatatcata agctgcaaga ttttggtgat gcgttaagaa ataaatatta cccacatata
     3481 ctatctgaag ctgtaatcca tgaaataaaa atagaaaaag gcattgacac ctcaagtaaa
     3541 aaaaacaaaa aggctttttt ggtcgatcag ttgaagcatc aggatgaaat tgaactttta
     3601 aggatggtct accaacataa tttttattta ctaggtgtaa ttagaagtga agtagaaaga
     3661 aagcgtaatc ttagagatga agggctgagt aagtcagaag tcgatatagt tattcatcac
     3721 gatagaaaat cagagagcga ttatggtcag cagactgcga aagctatact tgactctgat
     3781 tattttgtta agaataatca aggacaaagg gcgcacctta aggctaaggt taagcgtttt
     3841 attggcttga tacatgggct aaatggttta actccaacca cgtatgaaaa aggtatgtat
     3901 gcagctcatt cagcatctct gcaatctgca tgcctttctc gtcaagttgg tgctgctata
     3961 ttggatgtta atgggaatct gatttccgtt gggcgtaatg atgtacctaa attcaatggt
     4021 gggctttata gtgcggatga tggtgtcaat gatcatcgtt gtgtatacaa aggtggtaaa
     4081 tgttataatg aagttagaaa actaaagatt aaaagcaaaa tttcacaaat acttatgtcg
     4141 gaaagcgctt gtgaacttgg tgttaaatta acagaagatc aagcagagca actggctgat
     4201 gtaatatata aaaaaacacc agtatcttct attatagagt actctcgctc aattcatgca
     4261 gagatggatg ctattacatc attagctaga ctaggtaatg gaggtttttc tgataaagtt
     4321 ttatatacaa ccacatttcc ttgtcataac tgcgcgagac atattgtcgc tgttggaata
     4381 acaaaagttg tttatataga gccatatgaa aaaagcctcg ctttagaatt gcatgatgat
     4441 gctataaccg aggtgaatga gcatgggaag gttatatttg aatcctttga aggtgtatca
     4501 cctcgtaggt atcaaaaatt cttcttttct acagatgaaa ggaaagatag tttcggtaac
     4561 gcagaaaagt actccactaa atataaaaat cacattgata tacagtttat tgatagctat
     4621 ttagatttcg aaaatcgagt cgttgatatt tttagaacta acacaaatat tgattctggc
     4681 aatcatg
//
