LOCUS       NZ_CP080356             5207 bp    DNA     linear   CON 04-JUN-2024
DEFINITION  Klebsiella pneumoniae strain K191663 plasmid unnamed3, complete
            sequence.
ACCESSION   NZ_CP080356
VERSION     NZ_CP080356.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN20422817
            Assembly: GCF_019443545.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 5207)
  AUTHORS   Zhang,B. and Zhou,Y.
  TITLE     ST16 K. pneumoniae in China
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5207)
  AUTHORS   Zhang,B. and Zhou,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2021) foundation medicine, Southwest medical
            university, Longmatan District, Luzhou, Sichuan 646607, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: SOAPdenovo v. 2.04; unicycler v. 0.4.7
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 100.0x
            Sequencing Technology             :: Illumina HiSeq; PacBio RSII
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_019443545.1-RS_2024_06_04
            Annotation Date                   :: 06/04/2024 06:14:40
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,635
            Genes (coding)                    :: 5,506
            CDSs (with protein)               :: 5,506
            Genes (RNA)                       :: 133
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 93
            ncRNAs                            :: 15
            Pseudo Genes (total)              :: 129
            CDSs (without protein)            :: 129
            Pseudo Genes (ambiguous residues) :: 0 of 129
            Pseudo Genes (frameshifted)       :: 59 of 129
            Pseudo Genes (incomplete)         :: 69 of 129
            Pseudo Genes (internal stop)      :: 12 of 129
            Pseudo Genes (multiple problems)  :: 11 of 129
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP080356.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5207
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="K191663"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="unnamed3"
                     /geo_loc_name="China:wuxi"
                     /collection_date="2019-11-07"
     gene            complement(<1..135)
                     /locus_tag="K0P45_RS28895"
     CDS             complement(<1..135)
                     /locus_tag="K0P45_RS28895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_228751052.1"
                     /translation="MSSLLTASRDFEEKSKAQQQLTEERLKAAFSKHENVVKSELNASA
                     "
                     /name="MbeB family mobilization protein"
     gene            complement(<176..874)
                     /locus_tag="K0P45_RS29155"
                     /old_locus_tag="K0P45_26505"
                     /pseudo=""
     CDS             complement(<176..874)
                     /locus_tag="K0P45_RS29155"
                     /old_locus_tag="K0P45_26505"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(813..1154)
                     /locus_tag="K0P45_RS26525"
                     /old_locus_tag="K0P45_26510"
     CDS             complement(813..1154)
                     /locus_tag="K0P45_RS26525"
                     /old_locus_tag="K0P45_26510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006122514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_071599364.1"
                     /translation="MADKRNKMLTMWVTEDEHRRLLERCDGKQLAAWMRQTCLDEKPTR
                     AGRLPSISPALLRQLAGMGNNLNQIARKVNAGGAGHDRVQIVAALMAIDAGLERLRHAV
                     LEKGPDDDC"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            1768..1899
                     /locus_tag="K0P45_RS28900"
     CDS             1768..1899
                     /locus_tag="K0P45_RS28900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_072007956.1"
                     /translation="MHEPPVQSDCCALSGNYRLESNPKRHAKAPLAAATGNRIHGAE"
                     /name="replication initiation protein"
     gene            complement(2119..2928)
                     /locus_tag="K0P45_RS26530"
                     /old_locus_tag="K0P45_26515"
     CDS             complement(2119..2928)
                     /locus_tag="K0P45_RS26530"
                     /old_locus_tag="K0P45_26515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010222407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032446875.1"
                     /translation="MSKDKKERSKNRYHQLVEKIFFDKYVDGDLEVLFERLDINSAAKD
                     LGIELPSNVGDVIYSIRFRTPMPESILLTQPEGKEWIIELAGRSKYRFRLVTISRILPN
                     PNMVAIKIPDNTPEIITGYSLDDEQALLAKVRYNRLIDIFLGITTYSLQNHLRTTVKGI
                     GQIEIDELYVGIDKYGCHYIIPVQAKGGTDQISMVQTSQDTAWCEQKYPNVRCRAISAQ
                     FVSSDLIALFELKIDEGQVKLIEERHYKLVPVKELDSSDISNYRMSV"
                     /name="hypothetical protein"
     gene            complement(2921..4171)
                     /locus_tag="K0P45_RS26535"
                     /old_locus_tag="K0P45_26520"
     CDS             complement(2921..4171)
                     /locus_tag="K0P45_RS26535"
                     /old_locus_tag="K0P45_26520"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015160339.1"
                     /GO_function="GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO:0008168 - methyltransferase activity [Evidence IEA];
                     GO:1904047 - S-adenosyl-L-methionine binding [Evidence
                     IEA]"
                     /GO_process="GO:0006304 - DNA modification [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="WP_001584442.1"
                     /translation="MTRPIAVDLFAGAGGLSLGFEQAGFDIMASVEIDPIHCSAHKYNF
                     PMCKTICRSVVDVTGEYVREVSGIGEKEIDVVIGGAPCQGFSLIGKRALDDDRNKLVYH
                     YVRLVLELKPKYFVFENVKGLTVGKHKQFLVEIMDAFKDGGYDVVSDYKVLNAADYGVP
                     QDRRRLFLMGGRKGIALPSYPEPLSIRTTVGDAIGDIPDAEKYPELWGRDWVYAKFGEQ
                     SLYSSYLHGVGKDPSDFSYPRIFNKDKLTCSLLTDHSDISRGRFVETLPDTVEPISRFK
                     KLSMNGLCNTLRAGTASDRGAFTSPRPIHPIYPRVITVREAARLHSYPDWFRFHVTKWH
                     GFRQVGNSVPPMLARSVGLQIMKVLGVTPKKPTKKIKLGDESLVAISMSDAASMFGVSS
                     NVIAKRIKNSNKEVVCE"
                     /name="DNA cytosine methyltransferase"
     gene            complement(4623..4838)
                     /locus_tag="K0P45_RS26540"
                     /old_locus_tag="K0P45_26525"
     CDS             complement(4623..4838)
                     /locus_tag="K0P45_RS26540"
                     /old_locus_tag="K0P45_26525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015328239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_015328239.1"
                     /translation="MTELEKQLLSALEQLQQDYSRRLDEWESAFEEWRKMSGLMQRENA
                     ALSERVSGLSRQVQSLSEQLRRLSTG"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(4845..>5207)
                     /locus_tag="K0P45_RS26545"
                     /old_locus_tag="K0P45_26530"
     CDS             complement(4845..>5207)
                     /locus_tag="K0P45_RS26545"
                     /old_locus_tag="K0P45_26530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein"
                     /protein_id="WP_168800344.1"
                     /translation="KRINDAISAHEQGMNAAMQSNRLSVLRMVGRTWLTITMVSGLLFA
                     SLSGVLWYQGNRIAANLAEIRQQRDTLSKLHMQTWGVTYLENRNGRFLVLPEGMKAETG
                     RTVDNKTRNAVKLVRE"
                     /name="mobilization protein"
     Typing          complement(1239..1414)
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="90.449"
                     /coverage="81.0"
                     /evalue="5.74e-59"
     Typing          1361..1877
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="90.076"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          complement(3..823)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          complement(820..1133)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="92.453"
                     /coverage="99.0"
                     /evalue="1.04e-58"
     Typing          complement(4508..5205)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.571"
                     /coverage="99.0"
                     /evalue="9.98e-142"
ORIGIN
        1 cgcgcttgcg ttcagttccg atttgacaac gttctcgtgc ttgctgaatg cggctttcag
       61 cctctcttcg gtaagctgct gctgtgcttt cgatttctcc tcgaagtccc tcgaggccgt
      121 taaaagactg ctcataaatg gctcccctca gtcggatatt tcgccccccg tccgggtcgg
      181 caatgctgat actgtttttt gtggttctga ccacctcaaa accggccgct gtgagcgccc
      241 ctgtgacgtc atcacgcgtt ttcagctctc ctgatgaggc taacatcagc aaaccccgtg
      301 taatcgtctc tgcggcttcc tgcttcgttt ctggcagtcc tgaaggcgtg accagcgccc
      361 gccggttctc cgggtcgttc ggatcatgta gccccagcct gccgttgacc actgtctgcc
      421 acgcatcgat gcgcggccgg tcggcacgat cgtaatacgg ctggaggcgt ttccccgtca
      481 gtaattcggt attggggatc agaaaattca gctcaagacg acccttgtct gcgtgttcca
      541 cccacagaat gctgtactgg tctttatcga gtccgggcat cagaacccgc tcaaagctgg
      601 ccatcagttt ttcacgctga cccggcggca ggtccgcctc cgcgaatgac agcaccccgg
      661 aggtatattt tttcacatac ggcgacgcat caatcagctc ccggacttct tccggcttcc
      721 cctgcaggac gctggcacct tcacgctgcc ggtctttacc cagaagataa tccacaggac
      781 cggcgccccc gccgcgaccc cggggatgga atttaacaat catcgtccgg ccctttttcc
      841 agtacggcat gccgcaaccg ttccagaccg gcatcgatcg ccatcagcgc tgccacgatc
      901 tgcacccggt catgaccggc accaccggcg ttcactttcc gggcgatctg gttcaggtta
      961 ttgcccatgc cggcaagctg acgcagcagc gcgggcgaaa tcgagggaag cctgccggcg
     1021 cgtgtcggtt tctcatcaag gcaggtctgg cgcatccacg ccgccagttg ttttccgtcg
     1081 caccgctcaa gcaaccgccg gtgttcgtct tccgtcaccc acatcgtgag catcttgttg
     1141 cgtttatctg ccagaatcgc ctcctgataa cgccgtgacg gacgggaaag cctttcccgg
     1201 tcggcgcggg gttggacaag ccgcaggcgc gtcagtgggg tttcggggcg cagccctgaa
     1261 ccagtcacgt agcgctagcg gagtgtatac tggcttagta tgttacggcg agatggagat
     1321 tcggggaagt gcttcatgtg gcaaggggga aaatacggct gcggggcgta agtgcaaaat
     1381 gcaggtaacg atgaaatgac gcctcctcgc tcactcggtc gctgcgctcc tgccgtgaga
     1441 ctgcggcggg cgttaccagc tcacgaagga cgagataacg agatcgcgct caagtcagga
     1501 aagtcaggaa aaaggatgcg gcgttgccgt ttttccatag gctccgcccc cctgacaagc
     1561 atcacaaaat ctgacgctca aatcagtggt ggcgaaaccc gacaggacta taaagatccc
     1621 aggcgtttcc ccctggtagc tccctcgtgc gctctcctgt tcctgccttt cggtttaccg
     1681 gtgtcattcc gctgttatgg ccgcgtttgt ctcattccac gcctgacact cagttccggg
     1741 taggcagttc gctccaagct ggactgtatg cacgaacccc ccgttcagtc cgactgctgc
     1801 gccttgtccg gtaactatcg tcttgagtcc aacccgaaaa gacatgcaaa agcgccactg
     1861 gcagcagcca ctggtaatcg gattcacgga gcggagtaga gttatctaca gagttcttga
     1921 agtggtggcc tgactacggc tacactgaaa ggacagtttt ggtatctgcg ctccgtgaaa
     1981 gccagttacc ttcggaagaa agagttggta gctctaaatt ccggcaaacg aaccaccgtt
     2041 ggtagcggtg gtttttttgt ttacggctta ggagattacg gctagaacga aaggatctca
     2101 agaagatcct ctcagaaatt atacagacat gcggtagttt gagatgtcag agctatctaa
     2161 ctcttttacc ggaaccagtt tgtaatgcct ttcctcaatc aatttcactt gtccttcgtc
     2221 tatctttagc tcaaacagtg caatcaaatc gctcgaaaca aactgagcgg aaatagctct
     2281 acatctaaca tttgggtatt tttgttcaca ccacgctgta tcttgcgatg tttgcaccat
     2341 tgatatttga tctgtcccac cttttgcttg aactggtatt atataatggc aaccgtattt
     2401 atctatacct acataaagtt catcgatctc tatttgacca attcccttca ccgtagttct
     2461 taaatgattc tgtaaagaat atgtggttat acctagaaat atatctatta gccggttata
     2521 tctcaccttc gccagcaatg cctgctcgtc gtctaaagaa taacctgtaa ttatctccgg
     2581 ggtattgtct ggaattttta ttgccaccat attaggattt ggcaaaatcc tactaatagt
     2641 tactaatcta aatcgatact ttgacctacc agccagctca ataatccact ccttaccttc
     2701 tggctgagtc aacagaatac tttctggcat aggcgttcta aatctgattg aatatataac
     2761 atcaccaaca ttagatggga gttctatacc taaatctttt gcagcactgt ttatatctaa
     2821 cctttcaaac aaaacctcta agtcaccatc aacatattta tcgaaaaata tcttttctac
     2881 caactgatgg tatctatttt tggacctttc ttttttatcc ttactcacaa acaacctcct
     2941 tgttactatt ttttattctc ttagcaatta catttgatga cacaccaaac atagatgcag
     3001 catcggacat agaaatagca accaaacttt catcaccgag tttgattttc ttcgttggtt
     3061 tttttggcgt aacaccaaga accttcataa tctgcaagcc aacacttcta gctagcatag
     3121 gaggaacact attaccgacc tgtctaaatc catgccactt agtcacatga aatctgaacc
     3181 aatcaggata tgaatgcagt cttgctgcct ctctgaccgt gatgacccta ggatagatcg
     3241 gatgtatagg acgaggagat gtaaaagcac ctctgtcact tgctgttcct gcccttaaag
     3301 tattacatag cccattcatc gagagctttt taaacctgct gattggttct accgtgtcag
     3361 gcaaagtttc aacaaaccgc cctctagaaa tgtcagagtg atctgtcagt aaagaacacg
     3421 ttaatttgtc tttattaaaa atccttgggt agctaaaatc actaggatct tttcctacgc
     3481 catgcaaata ggatgagtat agtgattgct caccaaattt agcataaacc caatctctac
     3541 cccatagctc aggatatttt tccgcatcag gaatatcccc aatagcgtcc ccaaccgttg
     3601 ttctaatgga taatggctca gggtatgatg gcagagcaat tcctttcctt ccccccatta
     3661 agaaaagacg acggcgatct tgaggaactc cataatcagc tgcgtttagc actttataat
     3721 cgcttacaac gtcgtacccg ccatctttaa atgcatccat tatctctact aaaaactgct
     3781 tatgcttacc aaccgtcaac ccttttacat tttcaaaaac aaagtattta ggctttagct
     3841 caagaaccag ccgtacatag tgatagacca gtttgtttct atcatcatca agcgcgcgtt
     3901 ttcctattaa tgaaaagcct tggcagggag cgcctccgat cactacatca atctctttct
     3961 caccaatacc tgaaacttcc ctaacgtatt ctccggtaac atcaacaaca cttctacata
     4021 tcgttttgca cataggaaag ttatatttgt gagcagagca atgtattgga tctatttcaa
     4081 ctgatgccat tatatcaaag ccagcttgct cgaaaccgag actcagccca cccgctccag
     4141 caaataaatc aacagcaatc ggtctagtca ttctttacac ccacgttata taaaatcaac
     4201 atgatgctat cagaaaaacg atcgctttac cgatcgtttt tctgatttcg attgaacata
     4261 acatctctta cccgcaccgc gccggaggca ctcagggcgt ttctgatggc tgacgggcaa
     4321 aaggtggtgg caagtacacc gaaaaccacc agaaacgcac ctgatttatt ttacgcatat
     4381 gcatcactta ttcacatcac tgcatagccg gtgatttcgg ttaaaaaaga ctctctgtgt
     4441 gctgaaatcg cagaaaatgg ccatgatccc cgtcacgctc aggccacctg agacgggcgt
     4501 ttatcagtct ccatactccc tgtcaccatg agataatgtg cgttgccgtt ccggtaccag
     4561 atctgctgtc ttctctggtt ccttttccgt tgtttgcccg tatcgctgtt gctcaatctc
     4621 attcagcctg tcgataaccg gcgcagctgc tcgctcaaac tctgcacctg cctgctcaag
     4681 cccgacacgc gctcgctcag cgccgcgttc tcccgttgca taagaccaga catcttccgc
     4741 cattcctcga aggcgctctc ccactcgtcc agccttctcg agtagtcctg ctgtagctgc
     4801 tctaacgcgc tcaacaattg tttttccagt tccgtcattc tgctctactc ccgtaccagc
     4861 ttcaccgcgt tccgcgtctt gttgtccacc gtccggcccg tttcggcttt catcccctcc
     4921 ggcagcacca gaaaccgtcc gttgcggttc tccagatagg tcacgcccca cgtctgcatg
     4981 tgcagtttcg acagcgtatc tctctgctgc ctgatttccg ccaggttcgc cgctatccgg
     5041 ttcccctggt accacagcac cccgctcagg ctggcgaaca gcagccctga caccatggtg
     5101 atggtcagcc acgtccgccc gaccattcgc agcacgctca gccggttcga ctgcatcgcc
     5161 gcgttcatcc cctgttcgtg ggcgctgatg gcgtcgttga ttctctt
//
