LOCUS       NZ_CP080349             4822 bp    DNA     circular CON 10-JAN-2024
DEFINITION  Klebsiella pneumoniae strain K191659 plasmid pCol440I-191659,
            complete sequence.
ACCESSION   NZ_CP080349
VERSION     NZ_CP080349.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN20422816
            Assembly: GCF_019443565.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 4822)
  AUTHORS   Zhang,B. and Zhou,Y.
  TITLE     ST656 K. pneumoniae in China
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4822)
  AUTHORS   Zhang,B. and Zhou,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2021) foundation medicine, Southwest medical
            university, Longmatan District, Luzhou, Sichuan 646607, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: SOAPdenovo v. 2.04; unicycler v. 0.4.7
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 100.0x
            Sequencing Technology             :: Illumina HiSeq; PacBio RSII
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/10/2024 03:22:35
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,715
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,446
            CDSs (with protein)               :: 5,446
            Genes (RNA)                       :: 123
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 146
            CDSs (without protein)            :: 146
            Pseudo Genes (ambiguous residues) :: 0 of 146
            Pseudo Genes (frameshifted)       :: 70 of 146
            Pseudo Genes (incomplete)         :: 78 of 146
            Pseudo Genes (internal stop)      :: 23 of 146
            Pseudo Genes (multiple problems)  :: 22 of 146
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP080349.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4822
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="K191659"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pCol440I-191659"
                     /geo_loc_name="China:wuxi"
                     /collection_date="2019-10-24"
     gene            complement(join(4677..4822,1..256))
                     /locus_tag="K0P51_RS25650"
                     /old_locus_tag="K0P51_25650"
     CDS             complement(join(4677..4822,1..256))
                     /locus_tag="K0P51_RS25650"
                     /old_locus_tag="K0P51_25650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310048.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_064175096.1"
                     /translation="MARVSISEAARLTGKSRTTLHRLIKTGDLSTCSGERNAKMVDTSE
                     LLRVFGPFEQPKGEHHSGQVSEQRDTASADQSEQVIRHLKQEIEHLNTLLLSKDSHIDS
                     LKQAMLLLENHQAKLTESPTPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(256..447)
                     /locus_tag="K0P51_RS25655"
                     /old_locus_tag="K0P51_25655"
     CDS             complement(256..447)
                     /locus_tag="K0P51_RS25655"
                     /old_locus_tag="K0P51_25655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_004187666.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELDLDVEADLCEKLHDDA
                     EHLFRTLSSRLDALQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            858..971
                     /locus_tag="K0P51_RS28780"
                     /old_locus_tag="K0P51_25660"
     CDS             858..971
                     /locus_tag="K0P51_RS28780"
                     /old_locus_tag="K0P51_25660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032634168.1"
                     /translation="MHEPPVQSDCCALSGNYRLESNPERHAKAPLAAATGN"
                     /name="hypothetical protein"
     gene            1580..1873
                     /locus_tag="K0P51_RS25665"
                     /old_locus_tag="K0P51_25665"
     CDS             1580..1873
                     /locus_tag="K0P51_RS25665"
                     /old_locus_tag="K0P51_25665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012535809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_048290307.1"
                     /translation="MTIVISKEIEEKLTTKHKVTKKEVHESLANLNGNLLIDNREQHKT
                     NPPTNWFIAETNQRRELKVCFMIIDGDIHIKTAFEPNEIERKIYATHGKKTT"
                     /name="hypothetical protein"
     gene            2070..2438
                     /locus_tag="K0P51_RS25670"
                     /old_locus_tag="K0P51_25670"
     CDS             2070..2438
                     /locus_tag="K0P51_RS25670"
                     /old_locus_tag="K0P51_25670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014778268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_048290306.1"
                     /translation="MKTTSEFARDAQKWDDRELGASENHVEVASMADMSAFNDALSLQA
                     ISIRLQKNLVSDLKSIAESYGIGYQPMVRDLLQRFVIAERKQALRRELDKLNEREEIHQ
                     QEDTVPVDEFIESIRKQA"
                     /name="hypothetical protein"
     gene            2494..2622
                     /locus_tag="K0P51_RS28695"
     CDS             2494..2622
                     /locus_tag="K0P51_RS28695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012587681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_072201952.1"
                     /translation="MSLKPGPKRIADSTGQPDRRQRDNKKTPGNTDKLKPSKSSSK"
                     /name="hypothetical protein"
     gene            2692..2955
                     /locus_tag="K0P51_RS25675"
                     /old_locus_tag="K0P51_25675"
     CDS             2692..2955
                     /locus_tag="K0P51_RS25675"
                     /old_locus_tag="K0P51_25675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein L"
                     /protein_id="WP_072072086.1"
                     /translation="MAAHTSETVKFLQSVSSDQTWWKSRYNPGDSVPYSGIYRCTVCGK
                     EITSNGSDPFPPQNHHQHSQQQAIKWQLIVRTDTKGDRFGIK"
                     /name="protein L"
     gene            complement(3686..3949)
                     /locus_tag="K0P51_RS25680"
                     /old_locus_tag="K0P51_25680"
     CDS             complement(3686..3949)
                     /locus_tag="K0P51_RS25680"
                     /old_locus_tag="K0P51_25680"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_048290305.1"
                     /translation="MVNLNDICRSKKAAWIGGFFIFLARQNGIFDTNSGWLLLRVQFLS
                     RTVLRAVLLYLQLLRYSMGMYEADRGFQTFFITPFFYQQTWI"
                     /name="hypothetical protein"
     Typing          496..971
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="90.852"
                     /coverage="92.0"
                     /evalue="2.7e-180"
ORIGIN
        1 gtttcagatg acgaatcacc tgttcactct ggtcagcaga ggctgtatca cgctgttcag
       61 agacctgtcc agaatggtgt tcacccttcg gttgctcaaa gggaccaaag acacgtaaaa
      121 gctctgaagt atctaccatt tttgcattgc gctcaccact acaggtggac aggtcacctg
      181 ttttaattaa cctgtgcagt gttgtcctgc tcttacccgt cagtcgggca gcctcactaa
      241 tagacactct ggccatcagt taccatcctg taatgcatcc agtctcgagg acagtgtacg
      301 gaaaagatgc tccgcatcat cgtggagttt ctcacaaagg tcggcctcga catcgagatc
      361 aagctcgtta agcttctcca gcaggagcag agactgattc tggatgaatt tagccatgtt
      421 cagggcagtt ttctgttgtt tcgtcataag gggcctcagg gcgtcagtgc tcagtgtagg
      481 gatgttctct tttccgcttc ctcgctcact ccgtcgctac gctcctgccg ttcggctgcg
      541 gcgagcggac gggacacaca gaagagtcat cgcgataacc aggaagaaaa gagcctgtgg
      601 aaaaggccac ggcgttgccg tttttccata ggctccgccc ccctgacaag catcacagaa
      661 acggacgctc aaatcagcgg tggcgaaacc cgacaggact taaagatacc aggcgtttcc
      721 ccctggtggc tccctcgtgc gctctcctgt tcctgccttt cggtttaccg gtgtcattcc
      781 gctgttatgg ccgcgtttat ctcattccac gcctgacact cagttccggg aaggcagttc
      841 gctccaagct ggactgtatg cacgaacccc ccgttcagtc cgactgctgc gccttatccg
      901 gtaactatcg tcttgagtcc aacccggaaa gacatgcaaa agcgccactg gcagcagcca
      961 ctggtaactg atttagagga gatagtcttg aacttatgcg ccggttaagg ctaaactaaa
     1021 cgaacagttt tggtgactgt gctcctccaa gccagttacc tcggttcaaa gagttggtag
     1081 ctcagagaac cttcgaaaaa accgccttgc agggcggttt tttcgttttc acagcagtga
     1141 gattacgacg actaacacat gatctcaaga agatcctcag cctaagattg tgcctttttc
     1201 tttttgctat ctgcagcacc tttaacacga caaactctac accacgtctg aggagctacg
     1261 ttgccgcgta gatttctaaa accaaataga gcgtcaacct cttgataggt caaagctacg
     1321 actgggttca atcgataatc atagtcagaa caatgacatg aatgaggcaa tgctattgta
     1381 ttgctcataa cttcttctat atctatcttt cataaccaat atcaacggac caaaggctgc
     1441 gaagaagcaa tcgaaacagc ctatgtatat acacatagaa aatgaaaaca ccttaacaat
     1501 caagccatgg attgacaaat gtaaattttg tatatacaat gaattcaatt tcacaacaaa
     1561 ggccgaggca tcatgaaata tgacaattgt aatcagtaaa gaaattgaag aaaaactaac
     1621 cacaaaacat aaagtaacta aaaaagaagt acatgaaagc cttgcaaacc ttaacggtaa
     1681 cctgttgata gataacagag aacaacacaa aaccaatcct ccaaccaact ggtttatcgc
     1741 tgaaactaac cagcgtagag agttgaaagt gtgtttcatg attatcgatg gcgacattca
     1801 tatcaaaaca gccttcgaac cgaacgaaat agaacgtaaa atctatgcta cgcatggaaa
     1861 aaagacgaca taacagcgct aaataagtaa ttgttttgca aggccttacc atgtaagacc
     1921 ttgcaaaaat cttacatcag cgaaagaact aaaatgaaaa tgaagctgaa cctgatttct
     1981 caacatagaa gttcctttcg gaactagata aagcataaat attgcagtta atcctgatct
     2041 gcaggaaaca gcacctagag gtctatacaa tgaaaactac cagtgaattc gcacgtgatg
     2101 cacaaaaatg ggatgatcga gagctcggcg cctctgagaa tcacgtagaa gtagcatcga
     2161 tggctgacat gtcagctttc aacgatgcac tatcactgca agccatctca ataagattac
     2221 agaaaaatct tgtaagcgat ttaaaatcga tagctgaaag ctatggtata ggttatcaac
     2281 caatggttag agaccttctg caacgatttg ttattgcaga aagaaaacaa gcattacgta
     2341 gagagttgga caagctcaat gaacgagaag aaatacatca gcaggaggac acagttccag
     2401 ttgatgaatt cattgaaagc ataagaaaac aagcatgaaa gagcttacca tctagataga
     2461 aaaaatttca tcgcgtttga aaggaggatt atcatgagtc ttaaacctgg tccaaaaaga
     2521 atcgcagatt caacagggca acctgataga cgccaacgtg acaataaaaa aacaccggga
     2581 aatactgata aattaaaacc cagtaaatcc tcaagtaaat gaatcagtta aaagccgcct
     2641 tttaggcggc ttttcattgt cagtcctata aattcagaaa aggaaagcta aatggctgca
     2701 cataccagcg aaacagtgaa gtttttacaa agcgtcagta gcgatcaaac atggtggaag
     2761 tcacgatata atccaggaga cagcgttccc tattctggaa tttatcgatg tacagtttgc
     2821 ggaaaagaga tcaccagtaa cggcagcgac ccgttcccac cacaaaacca ccatcagcac
     2881 agtcagcaac aagcaataaa atggcaattg attgtcagaa cggacactaa aggtgaccgt
     2941 ttcggaatca aatgattgag agccctccgg ggcttttttt tgcacctgaa gctcgaagat
     3001 atttaccata acatcccagc cccgcacttc ttgtatgcta acaatcgtta cgctgcatga
     3061 gcgcacgaac gagatgcatg agtctttctc gccctccaaa aaagttaaac aaaaaaaaca
     3121 aatcagatta agtcattgta tttaatgcat taattaaaat ttacagcctt tagataatag
     3181 aaaaataaat taaaaatcaa tcacttaaaa aaatacacaa tttactttac ttgtttagtt
     3241 tgtcagttat tataaatgaa tattagttac tgatattaat ggtggttttt catggcggtt
     3301 gttgttatct atcgggctaa atgcatggct tgtcggcatg aatggactag taggagtggg
     3361 tttggaacac cgaactactg ccctaaatgt cgttcaaaat taattagttg cagtgcaatt
     3421 aagtaggtca aaatgacatc aaaacacaaa gggttatgtc ctttttgtaa agctgaagtt
     3481 atacctcgtg taattcaaga aaaccatgta cgacgagata agtgcgaatg ccctgagtgc
     3541 aaagaagcaa tttacatatg tcgcgctccg gggtgtgaga attacgctaa gggtggtcgt
     3601 ttgtatgacg atgagttgtg tccgtcatgt acatcaggta ttggcggcgg agtcggcaca
     3661 ttaggcatga tcgccttggg tgcagtcata tccacgtttg ttggtaaaaa aatggagtga
     3721 tgaagaatgt ctggaaacct cggtctgcct catacatgcc catgctgtaa cgtaacaact
     3781 gcaaatacag caggacagct ctcagcactg ttcggcttaa gaactgtacc ctcaggagca
     3841 accaaccaga gttggtgtcg aaaatgccgt tctgccgcgc caaaaaaatg aaaaagccgc
     3901 ctatccaggc ggcttttttg cttctgcaga tatcatttag atttaccata acgtcttctt
     3961 gtacgcaccg caaaaacggc ctcaaggcgt ttctgacggg aaaccggtaa aacaggcaac
     4021 aaaccacccg aaaacccgcc agaaacgcgc aggctctgtt tttacggcat gcatgagtat
     4081 gcacgcaagt gcatagcgcg aaaattcacg ccattcagcc agggtaagtc ctgtttccgg
     4141 cgttcttcct atccgaaaac ggtgaacggg gacgcaattt attgcgtggc cgtgaagcgt
     4201 tgccggcgca gccggaaaac cggttttcag cacgttcagg aatcgggttc gtcgacggcc
     4261 ggcaatcacg ttatggcaag tcgccggacg actcaccaca cgattcaccg gcgattcgaa
     4321 tcgtgaattg aatcaagagt caccatgaac aaaaaaaacc cagagtgatg aattgaatca
     4381 agagtcgatt catcataaca atatgataat aaagcgtgat tcacatatga atcgtgggca
     4441 attcgtcagc gattcgccat agagttgtcc acgacttgga tctgcacttt gtaacacatc
     4501 accgcacatt tatttttcat taacccattg ttgtaaataa ggaatctgca aaattttaat
     4561 tttgcgtggg gtgtgggtat ttttagtggt gaggagcgtt cagagatctg acgatttttg
     4621 ggaaggttca ggaactcccc gggatggccg gagaggaaaa ctgaagcatg acggggtcag
     4681 gattttttcc agaatttcca ccatggcgtc ggggattcag ttaattttgc ctggtgattt
     4741 tcaagaagga gcatggcctg tttcaggctg tcaatgtgcg aatccttgga caaaagtaag
     4801 gtgttcaggt gttcaatctc ct
//
