LOCUS       NZ_CP080315             5596 bp    DNA     circular CON 10-JAN-2024
DEFINITION  Klebsiella pneumoniae strain KP-426 plasmid unnamed2, complete
            sequence.
ACCESSION   NZ_CP080315
VERSION     NZ_CP080315.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN20373013
            Assembly: GCF_019434255.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 5596)
  AUTHORS   Chen,J. and Cai,J.
  TITLE     In vivo emergence of colistin and tigecycline resistance in
            carbapenem- resistant hypervirulent Klebsiella pneumoniae during
            antibiotics treatment
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5596)
  AUTHORS   Chen,J. and Cai,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUL-2021) Clinical Microbiology Laboratory, The Second
            Affiliated Hospital of Zhejiang University School of Medicine, 88
            Jiefang Rd, Hangzhou, Zhejiang Province 310009, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v.0.4.3
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 200.0x
            Sequencing Technology :: Illumina NovaSeq 6000; Nanopore PromethION
            48
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/10/2024 03:27:14
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,971
            CDSs (total)                      :: 5,850
            Genes (coding)                    :: 5,664
            CDSs (with protein)               :: 5,664
            Genes (RNA)                       :: 121
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 67 of 186
            Pseudo Genes (incomplete)         :: 124 of 186
            Pseudo Genes (internal stop)      :: 28 of 186
            Pseudo Genes (multiple problems)  :: 33 of 186
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP080315.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5596
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="KP-426"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="unnamed2"
                     /geo_loc_name="China:Hangzhou"
                     /collection_date="2020"
     gene            join(5477..5596,1..147)
                     /locus_tag="KZ481_RS29600"
                     /old_locus_tag="KZ481_29605"
     CDS             join(5477..5596,1..147)
                     /locus_tag="KZ481_RS29600"
                     /old_locus_tag="KZ481_29605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262326.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_004197762.1"
                     /translation="MVWTINYSDRALKSLRKMDKQNARRIVDFMSLRIAVAADPRQSGK
                     PLKGELGEFWRYRVGDYRVLCEIRDDELVILAATIGHRREVYD"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(217..567)
                     /locus_tag="KZ481_RS29605"
                     /old_locus_tag="KZ481_29610"
     CDS             complement(217..567)
                     /locus_tag="KZ481_RS29605"
                     /old_locus_tag="KZ481_29610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004197756.1"
                     /translation="MYWLIAFLAFIFPGRSFADGYTAYYRCNGTQEASLTVKDNKLSMV
                     VGLSKFNRQLDTYRFKEKGIEVVMQRAASDDNSEAIMSFIMPDVNNMTLLVKNNGYIIV
                     KTTCWLTKTDKQ"
                     /name="hypothetical protein"
     gene            complement(633..1178)
                     /locus_tag="KZ481_RS29610"
                     /old_locus_tag="KZ481_29615"
     CDS             complement(633..1178)
                     /locus_tag="KZ481_RS29610"
                     /old_locus_tag="KZ481_29615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_023317533.1"
                     /translation="MFFKRYLTEKEIYKAALPIVRLQGRVNTSLRFLSKYSDVNKNGLI
                     ALHLNLLHFISYNIHGVESLKITQAGINKAVQQSPHADEIKNIAQAIGRKGPEYIEGLI
                     RNPWGKKNDNPLIFIMSIEESIKETLNSGYMLYRVAPITEGEAAAFMDVLKQLLLFEDN
                     FRLFGRDLVYKIEKLKIR"
                     /name="hypothetical protein"
     gene            complement(1204..1530)
                     /locus_tag="KZ481_RS29615"
                     /old_locus_tag="KZ481_29620"
     CDS             complement(1204..1530)
                     /locus_tag="KZ481_RS29615"
                     /old_locus_tag="KZ481_29620"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_050516389.1"
                     /translation="MRERTNIMLDIGLKQSLQRLSNKTKMSLSDLINQRLSGSLSVEER
                     IGEKPEWMRNLDPYLANHFSNIELPLPSPLKEYQEKMDEIDSIINDMMVLKEYFRAKFY
                     DEIK"
                     /name="hypothetical protein"
     gene            complement(1888..2046)
                     /locus_tag="KZ481_RS30155"
     CDS             complement(1888..2046)
                     /locus_tag="KZ481_RS30155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071596352.1"
                     /translation="MHEPPVQPDCCALSGNYHLESNPDGHAKTPLAAAIGNWIYTREIL
                     RGLEVGA"
                     /name="replication initiation protein"
     gene            2686..2862
                     /locus_tag="KZ481_RS29620"
                     /old_locus_tag="KZ481_29625"
     CDS             2686..2862
                     /locus_tag="KZ481_RS29620"
                     /old_locus_tag="KZ481_29625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004197757.1"
                     /translation="MQKPKKKPLVVKKRSWHPAVMANLMRQAQEEDYRREQMAELERSA
                     RSHFADIGGEDGG"
                     /name="hypothetical protein"
     gene            2852..3196
                     /locus_tag="KZ481_RS29625"
                     /old_locus_tag="KZ481_29630"
     CDS             2852..3196
                     /locus_tag="KZ481_RS29625"
                     /old_locus_tag="KZ481_29630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006122514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_062955149.1"
                     /translation="MAVKRTRFLGIRVTDGEYQQLLERCNGRQLAVWMRETCLDTRPAR
                     SLRLPSIDPVLLRQLAGMGNNLNQIARKINGGQWSGADRVQVVAALMAIDAELERLRHA
                     VLEKGADDDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            3405..>3839
                     /locus_tag="KZ481_RS30030"
                     /pseudo=""
     CDS             3405..>3839
                     /locus_tag="KZ481_RS30030"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            3874..4383
                     /locus_tag="KZ481_RS29635"
                     /old_locus_tag="KZ481_29640"
     CDS             3874..4383
                     /locus_tag="KZ481_RS29635"
                     /old_locus_tag="KZ481_29640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_032433972.1"
                     /translation="MNSLLTLAKDLEQKSKAQQQSTGEMLKAAFSEHEKSVRAELSESG
                     KRISAAIRAHEQGMSAAMQSNRLSVMRMVGRTWLTITMVSGLLFASLSGVLWYQGSLIA
                     SNLAEIDRQNAALSKLNAKTWGVTYLEDSNGRFLVLPKGTAVDRTQSWTVGNGRSKQNA
                     LRLVKE"
                     /name="MbeB family mobilization protein"
     gene            4390..4602
                     /locus_tag="KZ481_RS29640"
                     /old_locus_tag="KZ481_29645"
     CDS             4390..4602
                     /locus_tag="KZ481_RS29640"
                     /old_locus_tag="KZ481_29645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_021527181.1"
                     /translation="MTELEKQLLSALEQLQQDYSKRLDEWESAFGEWRKMSGLMQRENA
                     ALSERVTRLSQQVERLSGQLQRLSR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            5269..5493
                     /locus_tag="KZ481_RS29645"
                     /old_locus_tag="KZ481_29650"
     CDS             5269..5493
                     /locus_tag="KZ481_RS29645"
                     /old_locus_tag="KZ481_29650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6290 family protein"
                     /protein_id="WP_000879771.1"
                     /translation="MLAIRLSDEIESRLDSLAKQTGRTKTFYAREAILAHLEDLEDYYL
                     SAETAARVRRGDEAVHSSEDVRKSLGLDD"
                     /name="DUF6290 family protein"
     Typing          2521..2600
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="90.361"
                     /coverage="97.0"
                     /evalue="1.1100000000000001e-21"
     Typing          complement(1938..2335)
                     /name="rep_cluster_2370"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="89.526"
                     /coverage="91.0"
                     /evalue="3.03e-139"
     Typing          3187..4718
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.804"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 cgccagtcag ggaagccgct caaaggtgag ctgggcgagt tctggcgcta tcgggtggga
       61 gattatcgcg ttctgtgtga gatccgagat gacgagcttg ttatccttgc cgccacgatt
      121 ggacatcgcc gcgaagttta cgactgaagc ccgctatgcg ggcttttttg atgccgacaa
      181 caggtattat ggtaaatact cgactaacta cgtgttctac tgtttgtcgg ttttagttaa
      241 ccagcaggtt gtcttaacga taatgtagcc gttattttta actaataagg tcatattgtt
      301 aacgtcaggc ataataaaag acattatggc ctcagagtta tcatcagaag cagctctctg
      361 cattactact tcgattccct tttctttgaa tctataagta tccagttgcc tattaaactt
      421 agaaagacct acaaccatac ttagtttgtt atctttaaca gtaagagatg cttcctgagt
      481 accattacat cggtagtaag cggtgtaacc gtcagcaaaa gatcgaccgg ggaatataaa
      541 tgctaggaac gcaattaacc aatacatagt tgagaatctt ggtattacac atttcactat
      601 cctctctttc atttttcgtt caccatactt tgttatctga tttttaattt ttctatttta
      661 taaaccaagt ctctaccaaa aaggcgaaaa ttatcctcaa ataataataa ttgtttaaga
      721 acatccatga aagcagctgc ttcaccctca gttatgggag ctactctata taacatataa
      781 cctgagttaa gggtttcttt tatactttct tcaatgctca ttataaatat tagtggatta
      841 tcatttttct tcccccatgg gtttctaatt aatccttcga tgtactcagg tcctttgcgt
      901 cctattgcct gcgcaatatt ttttatttca tctgcgtgag ggctttgttg tacagctttg
      961 tttatgcctg cttgagtgat tttcagagac tctacaccgt gtatgttgta tgagataaaa
     1021 tgtaataaat taagatgcaa tgcaattaat ccatttttgt ttacatctga gtatttggat
     1081 aaaaaacgta atgaagtgtt aactctccct tgaagcctaa caattgggag tgctgccttg
     1141 tatatttcct tctccgttaa ataccgttta aaaaacataa ttctccgcct ccgtatagcg
     1201 tgcctatttt atttcgtcgt aaaacttagc tctgaaatat tcctttagaa ccatcatgtc
     1261 attaataatt gaatctatct catccatttt ttcttggtat tcttttaatg gtgaagggag
     1321 cggtaactca atatttgaaa agtgattggc gagataggga tcaaggtttc tcatccattc
     1381 aggcttttcg ccaatgcgtt cttcaactga aagtgaacct gataatctct ggttaataag
     1441 atcacttaga ctcattttgg ttttattcga aagccgttgt aaggattgct ttaatcctat
     1501 atctagcatg atatttgttc tttctctcat taccccccct tgctattgcg ttttttgttg
     1561 cgcacaaagc taccattgcg cattaagaaa cgcaataaaa aaggagggag ggagtggttt
     1621 gtacatgatg tgcagtgcgc tgagggcatc agtggcgggt gtttttccgt gggatggatg
     1681 gagctccggg gataacagaa ctgacgatca tttaaaggat cttcttgaga tccttttttt
     1741 ctgcgcgtaa tctcttgcac tgtaaacgaa aaaaccgcct ggggaggcgg tttgatcgaa
     1801 ggttaagtca gttggggaac tgcttaaccg ggtaactggg tatacgagac cgcagatacc
     1861 aaaactgttc tttcagtgta gccgcagtta ggcacccact tcaagacctc tcaatatctc
     1921 tctcgtatat atccagttac caatggctgc tgccagtggc gtttttgcgt gtccgtccgg
     1981 gttggactca agatgatagt taccggaaag ggcgcagcag tcgggctgaa cggggggttc
     2041 gtgcatacag cccagcttgg agcgaacgac ctaaaccgag ccgagatacc agcagcgtga
     2101 gctatgagaa agcgccacgc ttcccgaaag ggaaaaaggc ggaacaggta tccggtaaac
     2161 ggcagggtcg gaacaggaga gcgcacgagg gagccgccgg agagaaatca ccggcatctt
     2221 taagtcctgt cgggtttcgt cctcctctga cttgagcgtc catttctgtg atgctcgtca
     2281 ggggaggcgg agcctatgga aaaacggcaa cgccgcatcc ttttctccgg ccctttctga
     2341 tttgagcgcc tttgctttat cccgttatcc gtgagtcggt cacgcccgcc gcagccgaac
     2401 ggcaggagcg cagctaccga gtgagcgagg aggcgtcatt tcatctgtcc gtgcatggta
     2461 ttgcggacgc tcctgagcca catatcacac ctgccacatg aggcactttc atgaatcgtg
     2521 ctctcatgcc aacatagtaa gccagtatac acagcgtaaa cgctgtgact ggttcagggc
     2581 tgcgccccga aacccgctaa caccactgac gcgctggcgc ttgtccaacg ccggagcgac
     2641 ccggaaaact tttccgtgcc gtccggcgtt tgcgaaggag ggataatgca gaagccgaag
     2701 aagaagccgc tcgtcgtaaa aaaaagatcc tggcatccgg cagtgatggc aaatctgatg
     2761 cgtcaggcac aggaggaaga ttaccgccgg gagcagatgg cagagcttga gcgttcagcc
     2821 cggtcacatt ttgcggatat cggaggagag gatggcggtt aagcgtaccc gttttttagg
     2881 tatccgggtg accgatgggg agtaccagca gctgctggag cgctgcaatg gaaggcaact
     2941 ggcggtgtgg atgcgggaaa cctgtctcga tacgcgcccg gcgcggtcgt tgcggctgcc
     3001 gtccatcgat ccggttttgc tgcgccagct tgccgggatg gggaacaacc tcaaccagat
     3061 cgcccggaaa attaacggcg gccagtggtc aggcgctgac cgggttcagg tggtggccgc
     3121 gctgatggcc attgatgccg aactcgagcg gctgcggcat gccgtgctgg aaaagggggc
     3181 agacgatgat cgttaagttt catccgcgag ggcgaggcgg cggtgccggg ccggtggatt
     3241 atctgctggg gaaagaccgc cagcgcgacg gggccatcgt cctgcaggga aagccggagg
     3301 aggtccggga gcttatcgac gcctcgccct acgccaaaaa gtacacctcc ggcgtcctgt
     3361 cttttgctga acaggattta ccgcccggcc agcgtgaaaa gctgatggcg agcttcgagc
     3421 gggttctgat gcccggactc gataaagacc agtacagcgt gctgtgggtt gaacaccggg
     3481 acaaggggcg gctggaactg aattttctgg taccgaacac ggaactgctg accggcaggc
     3541 gtctccagcc gtactacgac cgggcagacc gtccgcgcat cgatgcgtgg cagaccgtgg
     3601 tgaacgggcg actggggctg cacgacccga acgcaccgga gaaccggcgc gcgctggtga
     3661 cgccatcttc gttacccaaa acgaagcagg aagccgcaga ggctattacg cggggcttac
     3721 tggccctcgc ctcgtcaggg gagcttaaaa cgcgtcagga cgtcactgag gcgctggaaa
     3781 gcgcaggttt tgaggtcgtg cgcaccacaa agagcagcat cagcattgcc gacccggacg
     3841 gagggcgaaa catccgactt aagggagcca tctatgaaca gtcttttaac gctggcgaag
     3901 gacttagagc agaaatcgaa agcgcagcag cagagtaccg gcgaaatgct gaaagccgca
     3961 ttcagcgagc acgagaagtc tgtcagagcg gaactgagcg aaagcgggaa gagaatcagc
     4021 gccgccatcc gcgcccacga gcagggtatg agcgcggcca tgcagtcgaa ccgcctgagc
     4081 gtgatgcgta tggtcgggcg gacatggctg accatcacga tggtgtcagg actgctgttc
     4141 gccagcctga gcggggtgtt gtggtatcag gggagcttga tagcgtcaaa tctggccgaa
     4201 atcgaccggc agaacgcagc gctgtcgaag ctgaacgcga agacctgggg cgtgacgtac
     4261 ctggaagaca gcaacggacg ttttctggtg ctgccgaagg ggacggccgt agacaggacg
     4321 cagagctgga cggtcgggaa cgggcggtcg aagcagaacg cgctgagact ggtgaaggag
     4381 taagtgccga tgaccgagct ggaaaaacag ttgctgagcg cattagagca gctacagcag
     4441 gactactcga aaaggctgga cgagtgggag agcgccttcg gggaatggcg gaaaatgtca
     4501 gggcttatgc aacgggagaa cgcggcgctg agcgagcgcg tgacgcgctt gagtcagcag
     4561 gtggaacgct tgagcgggca actgcagcgt ttgagccggt agtgcagcgt catgagatgg
     4621 ccgtggccgc agaacgggcg cacgagcaac ggcagcacga aaaggaactg acggaagcgc
     4681 gttcccggca gcgaagctat gacggtccgt cgctgggctg aaatgcagga aaacagggtt
     4741 ctcatcgggc atttacgctg aaaccggatg tcattcacct ggctgcatgg ctatgcagcc
     4801 aggtaaaatt tatctgtcgc gaacggggcg gtttatcggg tggtttgttg ccggttttac
     4861 ctgactgcca ccccgttcgc ggcgaacccg tccggggcgg tgcgggcaac gggtgttatg
     4921 tttaatcagc gtgaacggca gatacaaaaa agccccggag ggcttttaac tactgactta
     4981 tttttttgaa gatttgctgg gtttcaattt gtcagtattt cccggcgttt ttttattgtc
     5041 acgctggcgt ttatcaggtt cccctgttga atcggcaatt cgtttcggac ccggcttaag
     5101 acccataatc gttatccttt caatagttaa gaggaaagcc ctcaagctag atatattgac
     5161 cgcgttacgg attatcaaga ggggctaaca agattatttt ttgttctttt aggttgattg
     5221 tggtactgtt gatatacatg tatagcaaat ggtgttcggg aggtttatat gcttgctatc
     5281 cggttatctg atgagattga gtcccgtctg gactcgctgg cgaagcaaac cggcagaaca
     5341 aagacgtttt atgcgcggga agcaatactg gcgcatctgg aagacctgga ggattattat
     5401 ctttcagcag aaactgctgc acgcgttcgc cgtggtgatg aagcagtgca ttcgtctgaa
     5461 gacgtgagga agtcacttgg tctggacgat taactattcc gatcgggcgc tcaaatcgtt
     5521 acgcaagatg gacaaacaga acgcacgacg gattgtggat tttatgagtt tacgcattgc
     5581 agttgctgcc gatcct
//
